Restriction map for
pF1KE0344
(Circular) (Six-base) MAPSORT of: pF1KE0344.seq Check: 5024 from: 1 to: 5234
pF1KE0344 5234 bp
With 182 enzymes: SgfI *
March 2, 2010 14:31 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 773 870 773
Size: 97 5137
AccI GT'mk_AC
Cuts at: 1076 2272 1076
Size: 1196 4038
Acc65I G'GTAC_C
Cuts at: 2250 2250
Size: 5234
AclI AA'CG_TT
Cuts at: 5074 5074
Size: 5234
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1210 3059 3491 4238 4506 1210
Size: 1849 432 747 268 1938
Fragments arranged by size:
1938 1849 747 432 268
AfeI AGC'GCT
Cuts at: 1789 1789
Size: 5234
AflIII A'CryG_T
Cuts at: 2586 3690 4667 2586
Size: 1104 977 3153
Fragments arranged by size:
3153 1104 977
AgeI A'CCGG_T
Cuts at: 3622 3622
Size: 5234
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 998 998
Size: 5234
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 1537 1569 1537
Size: 32 5202
AlwNI CAG_nnn'CTG
Cuts at: 276 339 677 732 885 897 978 1188 2070
Size: 63 338 55 153 12 81 210 882
Cuts at: 2070 2539 4106 276
Size: 469 1567 1404
Fragments arranged by size:
1567 1404 882 469 338 210 153 81
63 55 12
ApaI G_GGCC'C
Cuts at: 588 1416 1651 588
Size: 828 235 4171
Fragments arranged by size:
4171 828 235
ApaLI G'TGCA_C
Cuts at: 362 1102 4004 362
Size: 740 2902 1592
Fragments arranged by size:
2902 1592 740
ApoI r'AATT_y
Cuts at: 2238 4587 4843 2238
Size: 2349 256 2629
Fragments arranged by size:
2629 2349 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 5234
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 5234
AvaI C'yCGr_G
Cuts at: 214 1384 2254 214
Size: 1170 870 3194
Fragments arranged by size:
3194 1170 870
BamHI G'GATC_C
Cuts at: 2259 2259
Size: 5234
BanI G'GyrC_C
Cuts at: 1362 1397 1429 1599 1838 2106 2250 2664 2912
Size: 35 32 170 239 268 144 414 248
Cuts at: 2912 2947 1362
Size: 35 3649
Fragments arranged by size:
3649 414 268 248 239 170 144 35
35 32
BanII G_rGCy'C
Cuts at: 118 384 397 588 698 1385 1416 1651 1785
Size: 266 13 191 110 687 31 235 134
Cuts at: 1785 2033 2248 3278 118
Size: 248 215 1030 2074
Fragments arranged by size:
2074 1030 687 266 248 235 215 191
134 110 31 13
BbeI G_GCGC'C
Cuts at: 1401 1842 2668 2916 1401
Size: 441 826 248 3719
Fragments arranged by size:
3719 826 441 248
BbsI GAAGACnn'nnnn_
Cuts at: 153 605 1018 1198 153
Size: 452 413 180 4189
Fragments arranged by size:
4189 452 413 180
BbvCI CC'TCA_GC
Cuts at: 535 535
Size: 5234
BciVI GTATCCnnnnn_n'
Cuts at: 1757 3132 3893 5234 1757
Size: 1375 761 1341 1757
Fragments arranged by size:
1757 1375 1341 761
BclI T'GATC_A
Cuts at: 2754 2754
Size: 5234
BglI GCCn_nnn'nGGC
Cuts at: 209 218 458 774 1042 2047 209
Size: 9 240 316 268 1005 3396
Fragments arranged by size:
3396 1005 316 268 240 9
BglII A'GATC_T
Cuts at: 2749 4786 2749
Size: 2037 3197
BlpI GC'TnA_GC
Cuts at: 349 391 787 986 2346 349
Size: 42 396 199 1360 3237
Fragments arranged by size:
3237 1360 396 199 42
Bme1580I G_kGCm'C
Cuts at: 366 588 1106 1257 1330 1416 1604 1651 2859
Size: 222 518 151 73 86 188 47 1208
Cuts at: 2859 2952 4008 366
Size: 93 1056 1592
Fragments arranged by size:
1592 1208 1056 518 222 188 151 93
86 73 47
BmrI ACTGGGnnnn_n'
Cuts at: 325 720 1493 2446 2527 2862 5006 325
Size: 395 773 953 81 335 2144 553
Fragments arranged by size:
2144 953 773 553 395 335 81
BmtI G_CTAG'C
Cuts at: 2077 2077
Size: 5234
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3601 3601
Size: 5234
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 127 361 1134 1138 1534 1912 2026 2181 127
Size: 234 773 4 396 378 114 155 3180
Fragments arranged by size:
3180 773 396 378 234 155 114 4
Bpu10I CC'TnA_GC
Cuts at: 535 1576 1903 535
Size: 1041 327 3866
Fragments arranged by size:
3866 1041 327
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2410 3289 3781 4079 2410
Size: 879 492 298 3565
Fragments arranged by size:
3565 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 277 715 4938 277
Size: 438 4223 573
Fragments arranged by size:
4223 573 438
BsaAI yAC'GTr
Cuts at: 1377 2587 1377
Size: 1210 4024
BsaBI GATnn'nnATC
Cuts at: 6 904 2748 4785 6
Size: 898 1844 2037 455
Fragments arranged by size:
2037 1844 898 455
BsaHI Gr'CG_yC
Cuts at: 852 1398 1839 2665 2913 3615 5111 852
Size: 546 441 826 248 702 1496 975
Fragments arranged by size:
1496 975 826 702 546 441 248
BsaWI w'CCGG_w
Cuts at: 2090 2152 2423 2944 3622 3896 4043 4454 2090
Size: 62 271 521 678 274 147 411 2870
Fragments arranged by size:
2870 678 521 411 274 271 147 62
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1537 1567 1789 1819 2179 2209 1537
Size: 30 222 30 360 30 4562
Fragments arranged by size:
4562 360 222 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1867 2023 1867
Size: 156 5078
BseYI C'CCAG_C
Cuts at: 357 1218 1784 1808 2659 3417 3994 4490 357
Size: 861 566 24 851 758 577 496 1101
Fragments arranged by size:
1101 861 851 758 577 566 496 24
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 763 2083 763
Size: 1320 3914
BsiEI CG_ry'CG
Cuts at: 104 158 1337 1585 2776 2822 4030 104
Size: 54 1179 248 1191 46 1208 1308
Fragments arranged by size:
1308 1208 1191 1179 248 54 46
BsiHKAI G_wGCw'C
Cuts at: 366 1106 1442 2248 3026 3216 4008 366
Size: 740 336 806 778 190 792 1592
Fragments arranged by size:
1592 806 792 778 740 336 190
BsmBI CGTCTCn'nnnn_
Cuts at: 1888 1888
Size: 5234
Bsp1286I G_dGCh'C
Cuts at: 118 366 384 397 588 698 1106 1257 1330
Size: 248 18 13 191 110 408 151 73
Cuts at: 1330 1385 1416 1442 1604 1651 1785 2033 2248
Size: 55 31 26 162 47 134 248 215
Cuts at: 2248 2859 2952 3026 3216 3278 4008 118
Size: 611 93 74 190 62 730 1344
Fragments arranged by size:
1344 730 611 408 248 248 215 191
190 162 151 134 110 93 74 73
62 55 47 31 26 18 13
BspEI T'CCGG_A
Cuts at: 2152 4454 2152
Size: 2302 2932
BspHI T'CATG_A
Cuts at: 4410 4410
Size: 5234
BspMI ACCTGCnnnn'nnnn_
Cuts at: 773 870 882 2284 2498 2800 3181 773
Size: 97 12 1402 214 302 381 2826
Fragments arranged by size:
2826 1402 381 302 214 97 12
BsrBI CCG'CTC
Cuts at: 50 3526 3580 5064 5229 50
Size: 3476 54 1484 165 55
Fragments arranged by size:
3476 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 3146 3146
Size: 5234
BsrFI r'CCGG_y
Cuts at: 768 910 1580 3232 3413 3622 768
Size: 142 670 1652 181 209 2380
Fragments arranged by size:
2380 1652 670 209 181 142
BsrGI T'GTAC_A
Cuts at: 1559 1559
Size: 5234
BssSI C'ACGA_G
Cuts at: 3505 3863 3505
Size: 358 4876
BstAPI GCAn_nnn'nTGC
Cuts at: 885 2070 2611 885
Size: 1185 541 3508
Fragments arranged by size:
3508 1185 541
BstBI TT'CG_AA
Cuts at: 3595 3595
Size: 5234
BstYI r'GATC_y
Cuts at: 1 2259 2726 2749 3084 3330 4342 4428 4440
Size: 2258 467 23 335 246 1012 86 12
Cuts at: 4440 4786 1
Size: 346 449
Fragments arranged by size:
2258 1012 467 449 346 335 246 86
23 12
Bsu36I CC'TnA_GG
Cuts at: 251 651 1502 251
Size: 400 851 3983
Fragments arranged by size:
3983 851 400
BtgI C'CryG_G
Cuts at: 107 127 996 1337 1392 1433 1666 2042 107
Size: 20 869 341 55 41 233 376 3299
Fragments arranged by size:
3299 869 376 341 233 55 41 20
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 636 3184 3365 3528 636
Size: 2548 181 163 2342
Fragments arranged by size:
2548 2342 181 163
BtsI GCAGTG_nn'
Cuts at: 397 698 953 1328 2477 397
Size: 301 255 375 1149 3154
Fragments arranged by size:
3154 1149 375 301 255
DraI TTT'AAA
Cuts at: 2233 2233
Size: 5234
DraIII CAC_nnn'GTG
Cuts at: 1214 1214
Size: 5234
DrdI GACnn_nn'nnGTC
Cuts at: 2940 3798 2940
Size: 858 4376
EaeI y'GGCC_r
Cuts at: 155 515 797 993 1016 1043 1451 1530 1582
Size: 360 282 196 23 27 408 79 52
Cuts at: 1582 1739 2039 2819 2993 3384 3411 4756 155
Size: 157 300 780 174 391 27 1345 633
Fragments arranged by size:
1345 780 633 408 391 360 300 282
196 174 157 79 52 27 27 23
EagI C'GGCC_G
Cuts at: 155 1582 2819 155
Size: 1427 1237 2570
Fragments arranged by size:
2570 1427 1237
EarI CTCTTCn'nnn_
Cuts at: 142 1474 3257 3467 142
Size: 1332 1783 210 1909
Fragments arranged by size:
1909 1783 1332 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 625 1462 1708 3442 3752 3898 5044 625
Size: 837 246 1734 310 146 1146 815
Fragments arranged by size:
1734 1146 837 815 310 246 146
EcoICRI GAG'CTC
Cuts at: 2246 2246
Size: 5234
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 127 361 1134 1138 1210 1534 1912 2026 2181
Size: 234 773 4 72 324 378 114 155
Cuts at: 2181 3059 3491 4238 4506 127
Size: 878 432 747 268 855
Fragments arranged by size:
878 855 773 747 432 378 324 268
234 155 114 72 4
EcoNI CCTnn'n_nnAGG
Cuts at: 1696 1966 1696
Size: 270 4964
EcoO109I rG'GnC_Cy
Cuts at: 336 561 584 585 894 1035 1388 1412 1881
Size: 225 23 1 309 141 353 24 469
Cuts at: 1881 1971 2010 2067 2373 336
Size: 90 39 57 306 3197
Fragments arranged by size:
3197 469 353 309 306 225 141 90
57 39 24 23 1
EcoRI G'AATT_C
Cuts at: 2238 2238
Size: 5234
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 3253 3253
Size: 5234
FspI TGC'GCA
Cuts at: 3015 3015
Size: 5234
HaeII r_GCGC'y
Cuts at: 267 310 1401 1791 1842 1952 2668 2916 3938
Size: 43 1091 390 51 110 716 248 1022
Cuts at: 3938 4917 267
Size: 979 584
Fragments arranged by size:
1091 1022 979 716 584 390 248 110
51 43
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1974 2006 3076 3106 3108 3138 1974
Size: 32 1070 30 2 30 4070
Fragments arranged by size:
4070 1070 32 30 30 2
HincII GTy'rAC
Cuts at: 1125 2273 1125
Size: 1148 4086
KasI G'GCGC_C
Cuts at: 1397 1838 2664 2912 1397
Size: 441 826 248 3719
Fragments arranged by size:
3719 826 441 248
KpnI G_GTAC'C
Cuts at: 2254 2254
Size: 5234
MluI A'CGCG_T
Cuts at: 4667 4667
Size: 5234
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 944 1335 3905 4089 944
Size: 391 2570 184 2089
Fragments arranged by size:
2570 2089 391 184
MscI TGG'CCA
Cuts at: 799 995 1045 1453 1741 2995 799
Size: 196 50 408 288 1254 3038
Fragments arranged by size:
3038 1254 408 288 196 50
MslI CAynn'nnrTG
Cuts at: 2041 3223 3350 2041
Size: 1182 127 3925
Fragments arranged by size:
3925 1182 127
MspA1I CmG'CkG
Cuts at: 129 276 534 677 1449 1995 2103 2117 2345
Size: 147 258 143 772 546 108 14 228
Cuts at: 2345 2659 3019 4032 4277 129
Size: 314 360 1013 245 1086
Fragments arranged by size:
1086 1013 772 546 360 314 258 245
228 147 143 108 14
NaeI GCC'GGC
Cuts at: 1582 3415 1582
Size: 1833 3401
NarI GG'CG_CC
Cuts at: 1398 1839 2665 2913 1398
Size: 441 826 248 3719
Fragments arranged by size:
3719 826 441 248
NcoI C'CATG_G
Cuts at: 107 107
Size: 5234
NgoMIV G'CCGG_C
Cuts at: 1580 3413 1580
Size: 1833 3401
NheI G'CTAG_C
Cuts at: 2073 2073
Size: 5234
NspI r_CATG'y
Cuts at: 2287 2787 3694 2287
Size: 500 907 3827
Fragments arranged by size:
3827 907 500
PasI CC'CwG_GG
Cuts at: 333 375 567 1644 1875 333
Size: 42 192 1077 231 3692
Fragments arranged by size:
3692 1077 231 192 42
PciI A'CATG_T
Cuts at: 3690 3690
Size: 5234
PflMI CCAn_nnn'nTGG
Cuts at: 150 339 978 1025 1214 1848 150
Size: 189 639 47 189 634 3536
Fragments arranged by size:
3536 639 634 189 189 47
PmeI GTTT'AAAC
Cuts at: 2233 2233
Size: 5234
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 1537 1569 4373 4405 1537
Size: 32 2804 32 2366
Fragments arranged by size:
2804 2366 32 32
PpuMI rG'GwC_Cy
Cuts at: 894 1388 1881 894
Size: 494 493 4247
Fragments arranged by size:
4247 494 493
PspOMI G'GGCC_C
Cuts at: 584 1412 1647 584
Size: 828 235 4171
Fragments arranged by size:
4171 828 235
PstI C_TGCA'G
Cuts at: 893 1700 2281 2966 893
Size: 807 581 685 3161
Fragments arranged by size:
3161 807 685 581
PvuI CG_AT'CG
Cuts at: 104 104
Size: 5234
PvuII CAG'CTG
Cuts at: 276 1449 1995 2659 3019 276
Size: 1173 546 664 360 2491
Fragments arranged by size:
2491 1173 664 546 360
RsrII CG'GwC_CG
Cuts at: 3429 3429
Size: 5234
SacI G_AGCT'C
Cuts at: 2248 2248
Size: 5234
SacII CC_GC'GG
Cuts at: 130 130
Size: 5234
SalI G'TCGA_C
Cuts at: 2271 2271
Size: 5234
SanDI GG'GwC_CC
Cuts at: 1388 1881 1388
Size: 493 4741
SapI GCTCTTCn'nnn_
Cuts at: 142 3257 3467 142
Size: 3115 210 1909
Fragments arranged by size:
3115 1909 210
SbfI CC_TGCA'GG
Cuts at: 2281 2281
Size: 5234
ScaI AGT'ACT
Cuts at: 1798 4751 1798
Size: 2953 2281
SfcI C'TryA_G
Cuts at: 33 889 1304 1444 1696 1918 2277 2962 3955
Size: 856 415 140 252 222 359 685 993
Cuts at: 3955 4146 33
Size: 191 1121
Fragments arranged by size:
1121 993 856 685 415 359 252 222
191 140
SfiI GGCCn_nnn'nGGCC
Cuts at: 209 1042 209
Size: 833 4401
SfoI GGC'GCC
Cuts at: 1399 1840 2666 2914 1399
Size: 441 826 248 3719
Fragments arranged by size:
3719 826 441 248
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 5234
SmaI CCC'GGG
Cuts at: 1386 2256 1386
Size: 870 4364
SmlI C'TyrA_G
Cuts at: 2389 3304 3796 4058 2389
Size: 915 492 262 3565
Fragments arranged by size:
3565 915 492 262
SpeI A'CTAG_T
Cuts at: 67 366 67
Size: 299 4935
SphI G_CATG'C
Cuts at: 2287 2787 2287
Size: 500 4734
StuI AGG'CCT
Cuts at: 203 203
Size: 5234
StyI C'CwwG_G
Cuts at: 107 1031 2368 2722 107
Size: 924 1337 354 2619
Fragments arranged by size:
2619 1337 924 354
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 349 1210 1836 2817 2950 3409 3618 349
Size: 861 626 981 133 459 209 1965
Fragments arranged by size:
1965 981 861 626 459 209 133
TatI w'GTAC_w
Cuts at: 1559 1796 4749 1559
Size: 237 2953 2044
Fragments arranged by size:
2953 2044 237
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 1284 1316 4751 4783 1284
Size: 32 3435 32 1735
Fragments arranged by size:
3435 1735 32 32
Tth111I GACn'n_nGTC
Cuts at: 3031 3031
Size: 5234
XbaI T'CTAG_A
Cuts at: 2265 2265
Size: 5234
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 960 1038 1215 1423 1619 1959 2729 960
Size: 78 177 208 196 340 770 3465
Fragments arranged by size:
3465 770 340 208 196 177 78
XmaI C'CCGG_G
Cuts at: 1384 2254 1384
Size: 870 4364
Enzymes that do cut:
AarI AccI Acc65I AclI AcuI AfeI AflIII AgeI
AlfI AloI AlwNI ApaI ApaLI ApoI AseI AsiSI
AvaI BamHI BanI BanII BbeI BbsI BbvCI BciVI
BclI BglI BglII BlpI Bme1580I BmrI BmtI BplI
BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmBI Bsp1286I
BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssSI
BstAPI BstBI BstYI Bsu36I BtgI BtgZI BtsI DraI
DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI
EcoNI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII
KasI KpnI MluI MmeI MscI MslI MspA1I NaeI
NarI NcoI NgoMIV NheI NspI PasI PciI PflMI
PmeI PpiI PpuMI PspOMI PstI PvuI PvuII RsrII
SacI SacII SalI SanDI SapI SbfI ScaI SfcI
SfiI SfoI SgfI SmaI SmlI SpeI SphI StuI
StyI TaqII TatI TstI Tth111I XbaI XcmI XmaI
Enzymes that do not cut:
AatII AflII AhdI AleI AscI AvrII BaeI BcgI
BfrBI BmgBI BsiWI BsmI BssHII BstEII BstXI BstZ17I
ClaI CspCI EcoRV FseI FspAI HindIII HpaI MfeI
NdeI NotI NruI NsiI PacI PfoI PmlI PshAI
PsiI PspXI PsrI SexAI SgrAI SnaBI SrfI SspI
SwaI XhoI XmnI ZraI