Restriction map for
pF1KE0334
(Circular) (Six-base) MAPSORT of: pF1KE0334.seq Check: 4454 from: 1 to: 5738
pF1KE0334 5738 bp
With 182 enzymes: SgfI *
April 24, 2009 13:13 ..
AccI GT'mk_AC
Cuts at: 2776 2776
Size: 5738
Acc65I G'GTAC_C
Cuts at: 2754 2754
Size: 5738
AclI AA'CG_TT
Cuts at: 2732 5578 2732
Size: 2846 2892
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 752 1682 1773 3563 3995 4742 5010 752
Size: 930 91 1790 432 747 268 1480
Fragments arranged by size:
1790 1480 930 747 432 268 91
AfeI AGC'GCT
Cuts at: 812 812
Size: 5738
AflIII A'CryG_T
Cuts at: 801 1334 2609 3090 4194 5171 801
Size: 533 1275 481 1104 977 1368
Fragments arranged by size:
1368 1275 1104 977 533 481
AgeI A'CCGG_T
Cuts at: 4126 4126
Size: 5738
AleI CACnn'nnGTG
Cuts at: 2081 2081
Size: 5738
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 2690 2690
Size: 5738
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 841 873 841
Size: 32 5706
AlwNI CAG_nnn'CTG
Cuts at: 1104 1581 2175 3043 4610 1104
Size: 477 594 868 1567 2232
Fragments arranged by size:
2232 1567 868 594 477
ApaLI G'TGCA_C
Cuts at: 4508 4508
Size: 5738
ApoI r'AATT_y
Cuts at: 421 620 2006 2308 2461 2742 5091 5347 421
Size: 199 1386 302 153 281 2349 256 812
Fragments arranged by size:
2349 1386 812 302 281 256 199 153
AseI AT'TA_AT
Cuts at: 20 2702 20
Size: 2682 3056
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 5738
AvaI C'yCGr_G
Cuts at: 2758 2758
Size: 5738
BamHI G'GATC_C
Cuts at: 849 1613 2763 849
Size: 764 1150 3824
Fragments arranged by size:
3824 1150 764
BanI G'GyrC_C
Cuts at: 1749 2754 3168 3416 3451 1749
Size: 1005 414 248 35 4036
Fragments arranged by size:
4036 1005 414 248 35
BanII G_rGCy'C
Cuts at: 824 2752 3782 824
Size: 1928 1030 2780
Fragments arranged by size:
2780 1928 1030
BbeI G_GCGC'C
Cuts at: 3172 3420 3172
Size: 248 5490
BbsI GAAGACnn'nnnn_
Cuts at: 689 689
Size: 5738
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 349 383 349
Size: 34 5704
BciVI GTATCCnnnnn_n'
Cuts at: 778 3636 4397 5738 778
Size: 2858 761 1341 778
Fragments arranged by size:
2858 1341 778 761
BclI T'GATC_A
Cuts at: 531 2469 3258 531
Size: 1938 789 3011
Fragments arranged by size:
3011 1938 789
BfrBI ATG'CAT
Cuts at: 274 841 274
Size: 567 5171
BglII A'GATC_T
Cuts at: 3253 5290 3253
Size: 2037 3701
BlpI GC'TnA_GC
Cuts at: 1058 2850 1058
Size: 1792 3946
Bme1580I G_kGCm'C
Cuts at: 3363 3456 4512 3363
Size: 93 1056 4589
Fragments arranged by size:
4589 1056 93
BmrI ACTGGGnnnn_n'
Cuts at: 228 2950 3031 3366 5510 228
Size: 2722 81 335 2144 456
Fragments arranged by size:
2722 2144 456 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4105 4105
Size: 5738
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1102 2050 1102
Size: 948 4790
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 540 2914 3793 4285 4583 540
Size: 2374 879 492 298 1695
Fragments arranged by size:
2374 1695 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 5442 5442
Size: 5738
BsaAI yAC'GTr
Cuts at: 2610 3091 2610
Size: 481 5257
BsaBI GATnn'nnATC
Cuts at: 6 2072 2354 3252 5289 6
Size: 2066 282 898 2037 455
Fragments arranged by size:
2066 2037 898 455 282
BsaHI Gr'CG_yC
Cuts at: 3169 3417 4119 5615 3169
Size: 248 702 1496 3292
Fragments arranged by size:
3292 1496 702 248
BsaWI w'CCGG_w
Cuts at: 1871 2927 3448 4126 4400 4547 4958 1871
Size: 1056 521 678 274 147 411 2651
Fragments arranged by size:
2651 1056 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1669 1699 1669
Size: 30 5708
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1546 2318 1546
Size: 772 4966
BseYI C'CCAG_C
Cuts at: 124 158 2126 3163 3921 4498 4994 124
Size: 34 1968 1037 758 577 496 868
Fragments arranged by size:
1968 1037 868 758 577 496 34
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 392 1219 1882 2039 392
Size: 827 663 157 4091
Fragments arranged by size:
4091 827 663 157
BsiEI CG_ry'CG
Cuts at: 104 197 3280 3326 4534 104
Size: 93 3083 46 1208 1308
Fragments arranged by size:
3083 1308 1208 93 46
BsiHKAI G_wGCw'C
Cuts at: 556 2752 3530 3720 4512 556
Size: 2196 778 190 792 1782
Fragments arranged by size:
2196 1782 792 778 190
BsmI GAATG_Cn'
Cuts at: 902 902
Size: 5738
Bsp1286I G_dGCh'C
Cuts at: 556 824 2752 3363 3456 3530 3720 3782 4512
Size: 268 1928 611 93 74 190 62 730
Cuts at: 4512 556
Size: 1782
Fragments arranged by size:
1928 1782 730 611 268 190 93 74
62
BspEI T'CCGG_A
Cuts at: 1871 4958 1871
Size: 3087 2651
BspHI T'CATG_A
Cuts at: 534 4914 534
Size: 4380 1358
BspMI ACCTGCnnnn'nnnn_
Cuts at: 2788 3002 3304 3685 2788
Size: 214 302 381 4841
Fragments arranged by size:
4841 381 302 214
BsrBI CCG'CTC
Cuts at: 50 114 1990 4030 4084 5568 5733 50
Size: 64 1876 2040 54 1484 165 55
Fragments arranged by size:
2040 1876 1484 165 64 55 54
BsrDI GCAATG_nn'
Cuts at: 2041 2384 3650 2041
Size: 343 1266 4129
Fragments arranged by size:
4129 1266 343
BsrFI r'CCGG_y
Cuts at: 118 428 3736 3917 4126 118
Size: 310 3308 181 209 1730
Fragments arranged by size:
3308 1730 310 209 181
BsrGI T'GTAC_A
Cuts at: 1465 1828 2288 1465
Size: 363 460 4915
Fragments arranged by size:
4915 460 363
BssSI C'ACGA_G
Cuts at: 368 4009 4367 368
Size: 3641 358 1739
Fragments arranged by size:
3641 1739 358
BstAPI GCAn_nnn'nTGC
Cuts at: 280 3115 280
Size: 2835 2903
BstBI TT'CG_AA
Cuts at: 4099 4099
Size: 5738
BstXI CCAn_nnnn'nTGG
Cuts at: 264 1665 264
Size: 1401 4337
BstYI r'GATC_y
Cuts at: 1 849 1613 2349 2763 3230 3253 3588 3834
Size: 848 764 736 414 467 23 335 246
Cuts at: 3834 4846 4932 4944 5290 1
Size: 1012 86 12 346 449
Fragments arranged by size:
1012 848 764 736 467 449 414 346
335 246 86 23 12
BtgI C'CryG_G
Cuts at: 107 636 1652 2079 107
Size: 529 1016 427 3766
Fragments arranged by size:
3766 1016 529 427
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 3688 3869 4032 3688
Size: 181 163 5394
Fragments arranged by size:
5394 181 163
BtsI GCAGTG_nn'
Cuts at: 1075 1450 1646 2981 1075
Size: 375 196 1335 3832
Fragments arranged by size:
3832 1335 375 196
DraI TTT'AAA
Cuts at: 2737 2737
Size: 5738
DrdI GACnn_nn'nnGTC
Cuts at: 2610 3444 4302 2610
Size: 834 858 4046
Fragments arranged by size:
4046 858 834
EaeI y'GGCC_r
Cuts at: 1655 2715 3323 3497 3888 3915 5260 1655
Size: 1060 608 174 391 27 1345 2133
Fragments arranged by size:
2133 1345 1060 608 391 174 27
EagI C'GGCC_G
Cuts at: 3323 3323
Size: 5738
EarI CTCTTCn'nnn_
Cuts at: 929 3761 3971 929
Size: 2832 210 2696
Fragments arranged by size:
2832 2696 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 3946 4256 4402 5548 3946
Size: 310 146 1146 4136
Fragments arranged by size:
4136 1146 310 146
EcoICRI GAG'CTC
Cuts at: 2750 2750
Size: 5738
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 752 1102 1682 1773 2050 3563 3995 4742 5010
Size: 350 580 91 277 1513 432 747 268
Cuts at: 5010 752
Size: 1480
Fragments arranged by size:
1513 1480 747 580 432 350 277 268
91
EcoO109I rG'GnC_Cy
Cuts at: 2598 2670 2877 2598
Size: 72 207 5459
Fragments arranged by size:
5459 207 72
EcoRI G'AATT_C
Cuts at: 2742 2742
Size: 5738
EcoRV GAT'ATC
Cuts at: 647 647
Size: 5738
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 3757 3757
Size: 5738
FseI GG_CCGG'CC
Cuts at: 122 122
Size: 5738
FspI TGC'GCA
Cuts at: 3519 3519
Size: 5738
HaeII r_GCGC'y
Cuts at: 814 3172 3420 4442 5421 814
Size: 2358 248 1022 979 1131
Fragments arranged by size:
2358 1131 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 175 207 251 283 538 570 1669 1701 1714
Size: 32 44 32 255 32 1099 32 13
Cuts at: 1714 1746 3580 3610 3612 3642 175
Size: 32 1834 30 2 30 2271
Fragments arranged by size:
2271 1834 1099 255 44 32 32 32
32 32 30 30 13 2
HincII GTy'rAC
Cuts at: 873 2777 873
Size: 1904 3834
HindIII A'AGCT_T
Cuts at: 1586 1586
Size: 5738
KasI G'GCGC_C
Cuts at: 3168 3416 3168
Size: 248 5490
KpnI G_GTAC'C
Cuts at: 2758 2758
Size: 5738
MluI A'CGCG_T
Cuts at: 5171 5171
Size: 5738
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 4409 4593 4409
Size: 184 5554
MscI TGG'CCA
Cuts at: 1657 3499 1657
Size: 1842 3896
MslI CAynn'nnrTG
Cuts at: 304 596 635 1195 1627 2081 3727 3854 304
Size: 292 39 560 432 454 1646 127 2188
Fragments arranged by size:
2188 1646 560 454 432 292 127 39
MspA1I CmG'CkG
Cuts at: 2849 3163 3523 4536 4781 2849
Size: 314 360 1013 245 3806
Fragments arranged by size:
3806 1013 360 314 245
NaeI GCC'GGC
Cuts at: 120 3919 120
Size: 3799 1939
NarI GG'CG_CC
Cuts at: 3169 3417 3169
Size: 248 5490
NcoI C'CATG_G
Cuts at: 107 636 1652 107
Size: 529 1016 4193
Fragments arranged by size:
4193 1016 529
NdeI CA'TA_TG
Cuts at: 843 843
Size: 5738
NgoMIV G'CCGG_C
Cuts at: 118 3917 118
Size: 3799 1939
NsiI A_TGCA'T
Cuts at: 276 843 276
Size: 567 5171
NspI r_CATG'y
Cuts at: 805 1338 1960 1983 2791 3291 4198 805
Size: 533 622 23 808 500 907 2345
Fragments arranged by size:
2345 907 808 622 533 500 23
PciI A'CATG_T
Cuts at: 801 1334 4194 801
Size: 533 2860 2345
Fragments arranged by size:
2860 2345 533
PflMI CCAn_nnn'nTGG
Cuts at: 2070 2070
Size: 5738
PmeI GTTT'AAAC
Cuts at: 2737 2737
Size: 5738
PmlI CAC'GTG
Cuts at: 2610 2610
Size: 5738
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4877 4909 4877
Size: 32 5706
PsiI TTA'TAA
Cuts at: 2323 2323
Size: 5738
PstI C_TGCA'G
Cuts at: 2647 2785 3470 2647
Size: 138 685 4915
Fragments arranged by size:
4915 685 138
PvuI CG_AT'CG
Cuts at: 104 104
Size: 5738
PvuII CAG'CTG
Cuts at: 3163 3523 3163
Size: 360 5378
RsrII CG'GwC_CG
Cuts at: 3933 3933
Size: 5738
SacI G_AGCT'C
Cuts at: 2752 2752
Size: 5738
SalI G'TCGA_C
Cuts at: 2775 2775
Size: 5738
SapI GCTCTTCn'nnn_
Cuts at: 3761 3971 3761
Size: 210 5528
SbfI CC_TGCA'GG
Cuts at: 2785 2785
Size: 5738
ScaI AGT'ACT
Cuts at: 5255 5255
Size: 5738
SfcI C'TryA_G
Cuts at: 33 1726 2273 2643 2781 3466 4459 4650 33
Size: 1693 547 370 138 685 993 191 1121
Fragments arranged by size:
1693 1121 993 685 547 370 191 138
SfoI GGC'GCC
Cuts at: 3170 3418 3170
Size: 248 5490
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 5738
SmaI CCC'GGG
Cuts at: 2760 2760
Size: 5738
SmlI C'TyrA_G
Cuts at: 555 2893 3808 4300 4562 555
Size: 2338 915 492 262 1731
Fragments arranged by size:
2338 1731 915 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 5738
SphI G_CATG'C
Cuts at: 2791 3291 2791
Size: 500 5238
StyI C'CwwG_G
Cuts at: 107 523 636 1652 2872 3226 107
Size: 416 113 1016 1220 354 2619
Fragments arranged by size:
2619 1220 1016 416 354 113
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 183 3321 3454 3913 4122 183
Size: 3138 133 459 209 1799
Fragments arranged by size:
3138 1799 459 209 133
TatI w'GTAC_w
Cuts at: 1465 1828 2288 5253 1465
Size: 363 460 2965 1950
Fragments arranged by size:
2965 1950 460 363
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 1361 1393 1893 1925 5255 5287 1361
Size: 32 500 32 3330 32 1812
Fragments arranged by size:
3330 1812 500 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 193 3535 193
Size: 3342 2396
XbaI T'CTAG_A
Cuts at: 2769 2769
Size: 5738
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 261 1218 3233 261
Size: 957 2015 2766
Fragments arranged by size:
2766 2015 957
XmaI C'CCGG_G
Cuts at: 2758 2758
Size: 5738
XmnI GAAnn'nnTTC
Cuts at: 1577 1577
Size: 5738
Enzymes that do cut:
AccI Acc65I AclI AcuI AfeI AflIII AgeI AleI
AlfI AloI AlwNI ApaLI ApoI AseI AsiSI AvaI
BamHI BanI BanII BbeI BbsI BcgI BciVI BclI
BfrBI BglII BlpI Bme1580I BmrI BplI BpmI BpuEI
BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI
BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI
BsrBI BsrDI BsrFI BsrGI BssSI BstAPI BstBI BstXI
BstYI BtgI BtgZI BtsI DraI DrdI EaeI EagI
EarI EciI EcoICRI Eco57MI EcoO109I EcoRI EcoRV FalI
FseI FspI HaeII Hin4I HincII HindIII KasI KpnI
MluI MmeI MscI MslI MspA1I NaeI NarI NcoI
NdeI NgoMIV NsiI NspI PciI PflMI PmeI PmlI
PpiI PsiI PstI PvuI PvuII RsrII SacI SalI
SapI SbfI ScaI SfcI SfoI SgfI SmaI SmlI
SpeI SphI StyI TaqII TatI TstI Tth111I XbaI
XcmI XmaI XmnI
Enzymes that do not cut:
AarI AatII AflII AhdI ApaI AscI AvrII BaeI
BbvCI BglI BmgBI BmtI Bpu10I BsiWI BsmBI BssHII
BstEII BstZ17I Bsu36I ClaI CspCI DraIII EcoNI FspAI
HpaI MfeI NheI NotI NruI PacI PasI PfoI
PpuMI PshAI PspOMI PspXI PsrI SacII SanDI SexAI
SfiI SgrAI SnaBI SrfI SspI StuI SwaI XhoI
ZraI