Restriction map for
pF1KE0333
(Circular) (Six-base) MAPSORT of: pF1KE0333.seq Check: 1928 from: 1 to: 5012
pF1KE0333 5012 bp
With 182 enzymes: SgfI *
April 24, 2009 13:13 ..
AccI GT'mk_AC
Cuts at: 488 2050 488
Size: 1562 3450
Acc65I G'GTAC_C
Cuts at: 2028 2028
Size: 5012
AclI AA'CG_TT
Cuts at: 4852 4852
Size: 5012
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 688 1717 2837 3269 4016 4284 688
Size: 1029 1120 432 747 268 1416
Fragments arranged by size:
1416 1120 1029 747 432 268
AflIII A'CryG_T
Cuts at: 2364 3468 4445 2364
Size: 1104 977 2931
Fragments arranged by size:
2931 1104 977
AgeI A'CCGG_T
Cuts at: 3400 3400
Size: 5012
AlwNI CAG_nnn'CTG
Cuts at: 522 970 1161 2317 3884 522
Size: 448 191 1156 1567 1650
Fragments arranged by size:
1650 1567 1156 448 191
ApaLI G'TGCA_C
Cuts at: 3782 3782
Size: 5012
ApoI r'AATT_y
Cuts at: 886 1240 2016 4365 4621 886
Size: 354 776 2349 256 1277
Fragments arranged by size:
2349 1277 776 354 256
AseI AT'TA_AT
Cuts at: 20 1948 20
Size: 1928 3084
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 5012
AvaI C'yCGr_G
Cuts at: 2032 2032
Size: 5012
BamHI G'GATC_C
Cuts at: 879 2037 879
Size: 1158 3854
BanI G'GyrC_C
Cuts at: 2028 2442 2690 2725 2028
Size: 414 248 35 4315
Fragments arranged by size:
4315 414 248 35
BanII G_rGCy'C
Cuts at: 313 551 1346 1978 2026 3056 313
Size: 238 795 632 48 1030 2269
Fragments arranged by size:
2269 1030 795 632 238 48
BbeI G_GCGC'C
Cuts at: 2446 2694 2446
Size: 248 4764
BbsI GAAGACnn'nnnn_
Cuts at: 1476 1476
Size: 5012
BciVI GTATCCnnnnn_n'
Cuts at: 2910 3671 5012 2910
Size: 761 1341 2910
Fragments arranged by size:
2910 1341 761
BclI T'GATC_A
Cuts at: 2532 2532
Size: 5012
BglII A'GATC_T
Cuts at: 2527 4564 2527
Size: 2037 2975
BlpI GC'TnA_GC
Cuts at: 2124 2124
Size: 5012
Bme1580I G_kGCm'C
Cuts at: 359 1403 2637 2730 3786 359
Size: 1044 1234 93 1056 1585
Fragments arranged by size:
1585 1234 1056 1044 93
BmgBI CAC'GTC
Cuts at: 1170 1170
Size: 5012
BmrI ACTGGGnnnn_n'
Cuts at: 980 2224 2305 2640 4784 980
Size: 1244 81 335 2144 1208
Fragments arranged by size:
2144 1244 1208 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3379 3379
Size: 5012
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 544 682 727 1060 1138 1415 1519 544
Size: 138 45 333 78 277 104 4037
Fragments arranged by size:
4037 333 277 138 104 78 45
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1646 2188 3067 3559 3857 1646
Size: 542 879 492 298 2801
Fragments arranged by size:
2801 879 542 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 4716 4716
Size: 5012
BsaAI yAC'GTr
Cuts at: 2365 2365
Size: 5012
BsaBI GATnn'nnATC
Cuts at: 6 1232 2526 4563 6
Size: 1226 1294 2037 455
Fragments arranged by size:
2037 1294 1226 455
BsaHI Gr'CG_yC
Cuts at: 2443 2691 3393 4889 2443
Size: 248 702 1496 2566
Fragments arranged by size:
2566 1496 702 248
BsaWI w'CCGG_w
Cuts at: 2201 2722 3400 3674 3821 4232 2201
Size: 521 678 274 147 411 2981
Fragments arranged by size:
2981 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 655 685 655
Size: 30 4982
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1828 1828
Size: 5012
BseYI C'CCAG_C
Cuts at: 1064 1124 2437 3195 3772 4268 1064
Size: 60 1313 758 577 496 1808
Fragments arranged by size:
1808 1313 758 577 496 60
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 340 495 1108 340
Size: 155 613 4244
Fragments arranged by size:
4244 613 155
BsiEI CG_ry'CG
Cuts at: 104 1085 2554 2600 3808 104
Size: 981 1469 46 1208 1308
Fragments arranged by size:
1469 1308 1208 981 46
BsiHKAI G_wGCw'C
Cuts at: 470 505 551 1217 1346 1760 1978 2026 2804
Size: 35 46 666 129 414 218 48 778
Cuts at: 2804 2994 3786 470
Size: 190 792 1696
Fragments arranged by size:
1696 792 778 666 414 218 190 129
48 46 35
BsmI GAATG_Cn'
Cuts at: 717 1827 717
Size: 1110 3902
Bsp1286I G_dGCh'C
Cuts at: 313 359 470 505 551 1217 1346 1403 1760
Size: 46 111 35 46 666 129 57 357
Cuts at: 1760 1978 2026 2637 2730 2804 2994 3056 3786
Size: 218 48 611 93 74 190 62 730
Cuts at: 3786 313
Size: 1539
Fragments arranged by size:
1539 730 666 611 357 218 190 129
111 93 74 62 57 48 46 46
35
BspEI T'CCGG_A
Cuts at: 4232 4232
Size: 5012
BspHI T'CATG_A
Cuts at: 1788 4188 1788
Size: 2400 2612
BspMI ACCTGCnnnn'nnnn_
Cuts at: 2062 2276 2578 2959 2062
Size: 214 302 381 4115
Fragments arranged by size:
4115 381 302 214
BsrBI CCG'CTC
Cuts at: 50 3304 3358 4842 5007 50
Size: 3254 54 1484 165 55
Fragments arranged by size:
3254 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 1196 2924 1196
Size: 1728 3284
BsrFI r'CCGG_y
Cuts at: 818 3010 3191 3400 818
Size: 2192 181 209 2430
Fragments arranged by size:
2430 2192 209 181
BssSI C'ACGA_G
Cuts at: 3283 3641 3283
Size: 358 4654
BstAPI GCAn_nnn'nTGC
Cuts at: 2389 2389
Size: 5012
BstBI TT'CG_AA
Cuts at: 3373 3373
Size: 5012
BstEII G'GTnAC_C
Cuts at: 427 679 427
Size: 252 4760
BstXI CCAn_nnnn'nTGG
Cuts at: 129 129
Size: 5012
BstYI r'GATC_y
Cuts at: 1 449 879 2037 2504 2527 2862 3108 4120
Size: 448 430 1158 467 23 335 246 1012
Cuts at: 4120 4206 4218 4564 1
Size: 86 12 346 449
Fragments arranged by size:
1158 1012 467 449 448 430 346 335
246 86 23 12
Bsu36I CC'TnA_GG
Cuts at: 949 949
Size: 5012
BtgI C'CryG_G
Cuts at: 107 314 107
Size: 207 4805
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 428 2962 3143 3306 428
Size: 2534 181 163 2134
Fragments arranged by size:
2534 2134 181 163
BtsI GCAGTG_nn'
Cuts at: 1001 1034 1055 1580 2255 1001
Size: 33 21 525 675 3758
Fragments arranged by size:
3758 675 525 33 21
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 594 629 594
Size: 35 4977
DraI TTT'AAA
Cuts at: 1622 1842 1986 2011 1622
Size: 220 144 25 4623
Fragments arranged by size:
4623 220 144 25
DrdI GACnn_nn'nnGTC
Cuts at: 2718 3576 2718
Size: 858 4154
EaeI y'GGCC_r
Cuts at: 230 820 1082 2597 2771 3162 3189 4534 230
Size: 590 262 1515 174 391 27 1345 708
Fragments arranged by size:
1515 1345 708 590 391 262 174 27
EagI C'GGCC_G
Cuts at: 1082 2597 1082
Size: 1515 3497
EarI CTCTTCn'nnn_
Cuts at: 1298 3035 3245 1298
Size: 1737 210 3065
Fragments arranged by size:
3065 1737 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 3220 3530 3676 4822 3220
Size: 310 146 1146 3410
Fragments arranged by size:
3410 1146 310 146
EcoICRI GAG'CTC
Cuts at: 549 1344 1976 2024 549
Size: 795 632 48 3537
Fragments arranged by size:
3537 795 632 48
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 544 682 688 727 1060 1138 1415 1519 1717
Size: 138 6 39 333 78 277 104 198
Cuts at: 1717 2837 3269 4016 4284 544
Size: 1120 432 747 268 1272
Fragments arranged by size:
1272 1120 747 432 333 277 268 198
138 104 78 39 6
EcoNI CCTnn'n_nnAGG
Cuts at: 579 952 1185 579
Size: 373 233 4406
Fragments arranged by size:
4406 373 233
EcoO109I rG'GnC_Cy
Cuts at: 519 2151 519
Size: 1632 3380
EcoRI G'AATT_C
Cuts at: 1240 2016 1240
Size: 776 4236
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2019 3031 2019
Size: 1012 4000
FspI TGC'GCA
Cuts at: 2793 2793
Size: 5012
HaeII r_GCGC'y
Cuts at: 2446 2694 3716 4695 2446
Size: 248 1022 979 2763
Fragments arranged by size:
2763 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 261 293 847 879 1644 1676 2854 2884 2886
Size: 32 554 32 765 32 1178 30 2
Cuts at: 2886 2916 261
Size: 30 2357
Fragments arranged by size:
2357 1178 765 554 32 32 32 30
30 2
HincII GTy'rAC
Cuts at: 2051 2051
Size: 5012
KasI G'GCGC_C
Cuts at: 2442 2690 2442
Size: 248 4764
KpnI G_GTAC'C
Cuts at: 2032 2032
Size: 5012
MluI A'CGCG_T
Cuts at: 4445 4445
Size: 5012
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1733 3683 3867 1733
Size: 1950 184 2878
Fragments arranged by size:
2878 1950 184
MscI TGG'CCA
Cuts at: 232 2773 232
Size: 2541 2471
MslI CAynn'nnrTG
Cuts at: 343 3001 3128 343
Size: 2658 127 2227
Fragments arranged by size:
2658 2227 127
MspA1I CmG'CkG
Cuts at: 531 1081 1600 2123 2437 2797 3810 4055 531
Size: 550 519 523 314 360 1013 245 1488
Fragments arranged by size:
1488 1013 550 523 519 360 314 245
NaeI GCC'GGC
Cuts at: 3193 3193
Size: 5012
NarI GG'CG_CC
Cuts at: 2443 2691 2443
Size: 248 4764
NcoI C'CATG_G
Cuts at: 107 107
Size: 5012
NgoMIV G'CCGG_C
Cuts at: 3191 3191
Size: 5012
NspI r_CATG'y
Cuts at: 1177 2065 2565 3472 1177
Size: 888 500 907 2717
Fragments arranged by size:
2717 907 888 500
PasI CC'CwG_GG
Cuts at: 767 767
Size: 5012
PciI A'CATG_T
Cuts at: 3468 3468
Size: 5012
PflMI CCAn_nnn'nTGG
Cuts at: 384 522 1119 384
Size: 138 597 4277
Fragments arranged by size:
4277 597 138
PfoI T'CCnGG_A
Cuts at: 445 890 1154 1392 1496 445
Size: 445 264 238 104 3961
Fragments arranged by size:
3961 445 264 238 104
PmeI GTTT'AAAC
Cuts at: 2011 2011
Size: 5012
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4151 4183 4151
Size: 32 4980
PstI C_TGCA'G
Cuts at: 1298 1712 2059 2744 1298
Size: 414 347 685 3566
Fragments arranged by size:
3566 685 414 347
PvuI CG_AT'CG
Cuts at: 104 104
Size: 5012
PvuII CAG'CTG
Cuts at: 531 1600 2437 2797 531
Size: 1069 837 360 2746
Fragments arranged by size:
2746 1069 837 360
RsrII CG'GwC_CG
Cuts at: 3207 3207
Size: 5012
SacI G_AGCT'C
Cuts at: 551 1346 1978 2026 551
Size: 795 632 48 3537
Fragments arranged by size:
3537 795 632 48
SalI G'TCGA_C
Cuts at: 2049 2049
Size: 5012
SapI GCTCTTCn'nnn_
Cuts at: 3035 3245 3035
Size: 210 4802
SbfI CC_TGCA'GG
Cuts at: 2059 2059
Size: 5012
ScaI AGT'ACT
Cuts at: 4529 4529
Size: 5012
SfcI C'TryA_G
Cuts at: 33 1107 1294 1708 2055 2740 3733 3924 33
Size: 1074 187 414 347 685 993 191 1121
Fragments arranged by size:
1121 1074 993 685 414 347 191 187
SfoI GGC'GCC
Cuts at: 2444 2692 2444
Size: 248 4764
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 5012
SmaI CCC'GGG
Cuts at: 2034 2034
Size: 5012
SmlI C'TyrA_G
Cuts at: 1661 2167 3082 3574 3836 1661
Size: 506 915 492 262 2837
Fragments arranged by size:
2837 915 506 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 5012
SphI G_CATG'C
Cuts at: 1177 2065 2565 1177
Size: 888 500 3624
Fragments arranged by size:
3624 888 500
SspI AAT'ATT
Cuts at: 852 1928 852
Size: 1076 3936
StuI AGG'CCT
Cuts at: 585 585
Size: 5012
StyI C'CwwG_G
Cuts at: 107 332 580 1895 2146 2500 107
Size: 225 248 1315 251 354 2619
Fragments arranged by size:
2619 1315 354 251 248 225
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 377 2595 2728 3187 3396 377
Size: 2218 133 459 209 1993
Fragments arranged by size:
2218 1993 459 209 133
TatI w'GTAC_w
Cuts at: 4527 4527
Size: 5012
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 4529 4561 4529
Size: 32 4980
Tth111I GACn'n_nGTC
Cuts at: 2809 2809
Size: 5012
XbaI T'CTAG_A
Cuts at: 2043 2043
Size: 5012
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 635 2507 635
Size: 1872 3140
XmaI C'CCGG_G
Cuts at: 2032 2032
Size: 5012
XmnI GAAnn'nnTTC
Cuts at: 805 1252 805
Size: 447 4565
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AgeI AlwNI ApaLI
ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI
BbsI BciVI BclI BglII BlpI Bme1580I BmgBI BmrI
BplI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I
BspEI BspHI BspMI BsrBI BsrDI BsrFI BssSI BstAPI
BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI
CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI
Eco57MI EcoNI EcoO109I EcoRI FalI FspI HaeII Hin4I
HincII KasI KpnI MluI MmeI MscI MslI MspA1I
NaeI NarI NcoI NgoMIV NspI PasI PciI PflMI
PfoI PmeI PpiI PstI PvuI PvuII RsrII SacI
SalI SapI SbfI ScaI SfcI SfoI SgfI SmaI
SmlI SpeI SphI SspI StuI StyI TaqII TatI
TstI Tth111I XbaI XcmI XmaI XmnI
Enzymes that do not cut:
AarI AatII AfeI AflII AhdI AleI AlfI AloI
ApaI AscI AvrII BaeI BbvCI BcgI BfrBI BglI
BmtI Bpu10I BsiWI BsmBI BsrGI BssHII BstZ17I ClaI
DraIII EcoRV FseI FspAI HindIII HpaI MfeI NdeI
NheI NotI NruI NsiI PacI PmlI PpuMI PshAI
PsiI PspOMI PspXI PsrI SacII SanDI SexAI SfiI
SgrAI SnaBI SrfI SwaI XhoI ZraI