Restriction map for
pF1KE0332
(Circular) (Six-base) MAPSORT of: pF1KE0332.seq Check: 7015 from: 1 to: 4991
pF1KE0332 4991 bp
With 182 enzymes: SgfI *
April 24, 2009 13:13 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 138 1047 138
Size: 909 4082
AccI GT'mk_AC
Cuts at: 2029 2029
Size: 4991
Acc65I G'GTAC_C
Cuts at: 2007 2007
Size: 4991
AclI AA'CG_TT
Cuts at: 4831 4831
Size: 4991
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 2816 3248 3995 4263 2816
Size: 432 747 268 3544
Fragments arranged by size:
3544 747 432 268
AflIII A'CryG_T
Cuts at: 199 1252 2343 3447 4424 199
Size: 1053 1091 1104 977 766
Fragments arranged by size:
1104 1091 1053 977 766
AgeI A'CCGG_T
Cuts at: 317 3379 317
Size: 3062 1929
AlwNI CAG_nnn'CTG
Cuts at: 153 1549 2296 3863 153
Size: 1396 747 1567 1281
Fragments arranged by size:
1567 1396 1281 747
ApaLI G'TGCA_C
Cuts at: 3761 3761
Size: 4991
ApoI r'AATT_y
Cuts at: 1575 1995 4344 4600 1575
Size: 420 2349 256 1966
Fragments arranged by size:
2349 1966 420 256
AseI AT'TA_AT
Cuts at: 20 937 20
Size: 917 4074
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4991
AvaI C'yCGr_G
Cuts at: 2011 2011
Size: 4991
AvrII C'CTAG_G
Cuts at: 483 483
Size: 4991
BamHI G'GATC_C
Cuts at: 179 2016 179
Size: 1837 3154
BanI G'GyrC_C
Cuts at: 2007 2421 2669 2704 2007
Size: 414 248 35 4294
Fragments arranged by size:
4294 414 248 35
BanII G_rGCy'C
Cuts at: 2005 3035 2005
Size: 1030 3961
BbeI G_GCGC'C
Cuts at: 2425 2673 2425
Size: 248 4743
BbsI GAAGACnn'nnnn_
Cuts at: 145 645 998 145
Size: 500 353 4138
Fragments arranged by size:
4138 500 353
BciVI GTATCCnnnnn_n'
Cuts at: 1386 2889 3650 4991 1386
Size: 1503 761 1341 1386
Fragments arranged by size:
1503 1386 1341 761
BclI T'GATC_A
Cuts at: 2511 2511
Size: 4991
BglI GCCn_nnn'nGGC
Cuts at: 112 112
Size: 4991
BglII A'GATC_T
Cuts at: 2506 4543 2506
Size: 2037 2954
BlpI GC'TnA_GC
Cuts at: 2103 2103
Size: 4991
Bme1580I G_kGCm'C
Cuts at: 2616 2709 3765 2616
Size: 93 1056 3842
Fragments arranged by size:
3842 1056 93
BmgBI CAC'GTC
Cuts at: 135 227 468 660 135
Size: 92 241 192 4466
Fragments arranged by size:
4466 241 192 92
BmrI ACTGGGnnnn_n'
Cuts at: 1111 2203 2284 2619 4763 1111
Size: 1092 81 335 2144 1339
Fragments arranged by size:
2144 1339 1092 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 1669 3358 1669
Size: 1689 3302
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 315 1403 1432 1664 1743 1833 315
Size: 1088 29 232 79 90 3473
Fragments arranged by size:
3473 1088 232 90 79 29
Bpu10I CC'TnA_GC
Cuts at: 1187 1187
Size: 4991
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 715 1322 2167 3046 3538 3836 715
Size: 607 845 879 492 298 1870
Fragments arranged by size:
1870 879 845 607 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 4695 4695
Size: 4991
BsaAI yAC'GTr
Cuts at: 200 1255 2344 200
Size: 1055 1089 2847
Fragments arranged by size:
2847 1089 1055
BsaBI GATnn'nnATC
Cuts at: 6 2505 4542 6
Size: 2499 2037 455
Fragments arranged by size:
2499 2037 455
BsaHI Gr'CG_yC
Cuts at: 122 2422 2670 3372 4868 122
Size: 2300 248 702 1496 245
Fragments arranged by size:
2300 1496 702 248 245
BsaWI w'CCGG_w
Cuts at: 317 2180 2701 3379 3653 3800 4211 317
Size: 1863 521 678 274 147 411 1097
Fragments arranged by size:
1863 1097 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 238 268 766 796 1631 1661 1817 1847 238
Size: 30 498 30 835 30 156 30 3382
Fragments arranged by size:
3382 835 498 156 30 30 30 30
BseYI C'CCAG_C
Cuts at: 2416 3174 3751 4247 2416
Size: 758 577 496 3160
Fragments arranged by size:
3160 758 577 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 166 260 671 1859 166
Size: 94 411 1188 3298
Fragments arranged by size:
3298 1188 411 94
BsiEI CG_ry'CG
Cuts at: 104 318 1782 2533 2579 3787 104
Size: 214 1464 751 46 1208 1308
Fragments arranged by size:
1464 1308 1208 751 214 46
BsiHKAI G_wGCw'C
Cuts at: 2005 2783 2973 3765 2005
Size: 778 190 792 3231
Fragments arranged by size:
3231 792 778 190
BsmI GAATG_Cn'
Cuts at: 1306 1436 1306
Size: 130 4861
Bsp1286I G_dGCh'C
Cuts at: 2005 2616 2709 2783 2973 3035 3765 2005
Size: 611 93 74 190 62 730 3231
Fragments arranged by size:
3231 730 611 190 93 74 62
BspEI T'CCGG_A
Cuts at: 4211 4211
Size: 4991
BspHI T'CATG_A
Cuts at: 4167 4167
Size: 4991
BspMI ACCTGCnnnn'nnnn_
Cuts at: 138 1047 2041 2255 2557 2938 138
Size: 909 994 214 302 381 2191
Fragments arranged by size:
2191 994 909 381 302 214
BsrBI CCG'CTC
Cuts at: 50 3283 3337 4821 4986 50
Size: 3233 54 1484 165 55
Fragments arranged by size:
3233 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 1265 2903 1265
Size: 1638 3353
BsrFI r'CCGG_y
Cuts at: 317 2989 3170 3379 317
Size: 2672 181 209 1929
Fragments arranged by size:
2672 1929 209 181
BssSI C'ACGA_G
Cuts at: 643 3262 3620 643
Size: 2619 358 2014
Fragments arranged by size:
2619 2014 358
BstAPI GCAn_nnn'nTGC
Cuts at: 153 2368 153
Size: 2215 2776
BstBI TT'CG_AA
Cuts at: 1530 3352 1530
Size: 1822 3169
BstYI r'GATC_y
Cuts at: 1 179 1416 2016 2483 2506 2841 3087 4099
Size: 178 1237 600 467 23 335 246 1012
Cuts at: 4099 4185 4197 4543 1
Size: 86 12 346 449
Fragments arranged by size:
1237 1012 600 467 449 346 335 246
178 86 23 12
Bsu36I CC'TnA_GG
Cuts at: 409 409
Size: 4991
BtgI C'CryG_G
Cuts at: 1608 1608
Size: 4991
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 2941 3122 3285 2941
Size: 181 163 4647
Fragments arranged by size:
4647 181 163
BtsI GCAGTG_nn'
Cuts at: 243 1889 2234 243
Size: 1646 345 3000
Fragments arranged by size:
3000 1646 345
DraI TTT'AAA
Cuts at: 1990 1990
Size: 4991
DraIII CAC_nnn'GTG
Cuts at: 230 230
Size: 4991
DrdI GACnn_nn'nnGTC
Cuts at: 2697 3555 2697
Size: 858 4133
EaeI y'GGCC_r
Cuts at: 113 216 762 1587 1779 2576 2750 3141 3168
Size: 103 546 825 192 797 174 391 27
Cuts at: 3168 4513 113
Size: 1345 591
Fragments arranged by size:
1345 825 797 591 546 391 192 174
103 27
EagI C'GGCC_G
Cuts at: 1779 2576 1779
Size: 797 4194
EarI CTCTTCn'nnn_
Cuts at: 268 3014 3224 268
Size: 2746 210 2035
Fragments arranged by size:
2746 2035 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 1594 3199 3509 3655 4801 1594
Size: 1605 310 146 1146 1784
Fragments arranged by size:
1784 1605 1146 310 146
EcoICRI GAG'CTC
Cuts at: 2003 2003
Size: 4991
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 315 1403 1432 1664 1743 1833 2816 3248 3995
Size: 1088 29 232 79 90 983 432 747
Cuts at: 3995 4263 315
Size: 268 1043
Fragments arranged by size:
1088 1043 983 747 432 268 232 90
79 29
EcoNI CCTnn'n_nnAGG
Cuts at: 487 538 487
Size: 51 4940
EcoO109I rG'GnC_Cy
Cuts at: 479 2130 479
Size: 1651 3340
EcoRI G'AATT_C
Cuts at: 1995 1995
Size: 4991
EcoRV GAT'ATC
Cuts at: 1863 1863
Size: 4991
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 3010 3010
Size: 4991
FspI TGC'GCA
Cuts at: 2772 2772
Size: 4991
HaeII r_GCGC'y
Cuts at: 2425 2673 3695 4674 2425
Size: 248 1022 979 2742
Fragments arranged by size:
2742 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 238 270 565 597 1053 1085 1381 1413 1459
Size: 32 295 32 456 32 296 32 46
Cuts at: 1459 1491 2833 2863 2865 2895 238
Size: 32 1342 30 2 30 2334
Fragments arranged by size:
2334 1342 456 296 295 46 32 32
32 32 32 30 30 2
HincII GTy'rAC
Cuts at: 465 2030 465
Size: 1565 3426
KasI G'GCGC_C
Cuts at: 2421 2669 2421
Size: 248 4743
KpnI G_GTAC'C
Cuts at: 2011 2011
Size: 4991
MfeI C'AATT_G
Cuts at: 926 1443 926
Size: 517 4474
MluI A'CGCG_T
Cuts at: 4424 4424
Size: 4991
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 3662 3846 3662
Size: 184 4807
MscI TGG'CCA
Cuts at: 115 218 764 1589 2752 115
Size: 103 546 825 1163 2354
Fragments arranged by size:
2354 1163 825 546 103
MslI CAynn'nnrTG
Cuts at: 2980 3107 2980
Size: 127 4864
MspA1I CmG'CkG
Cuts at: 244 1843 2102 2416 2776 3789 4034 244
Size: 1599 259 314 360 1013 245 1201
Fragments arranged by size:
1599 1201 1013 360 314 259 245
NaeI GCC'GGC
Cuts at: 3172 3172
Size: 4991
NarI GG'CG_CC
Cuts at: 2422 2670 2422
Size: 248 4743
NdeI CA'TA_TG
Cuts at: 1080 1080
Size: 4991
NgoMIV G'CCGG_C
Cuts at: 3170 3170
Size: 4991
NspI r_CATG'y
Cuts at: 343 509 1489 2044 2544 3451 343
Size: 166 980 555 500 907 1883
Fragments arranged by size:
1883 980 907 555 500 166
PciI A'CATG_T
Cuts at: 3447 3447
Size: 4991
PflMI CCAn_nnn'nTGG
Cuts at: 771 771
Size: 4991
PmeI GTTT'AAAC
Cuts at: 1990 1990
Size: 4991
PmlI CAC'GTG
Cuts at: 200 1255 200
Size: 1055 3936
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 1112 1144 4130 4162 1112
Size: 32 2986 32 1941
Fragments arranged by size:
2986 1941 32 32
PpuMI rG'GwC_Cy
Cuts at: 479 479
Size: 4991
PstI C_TGCA'G
Cuts at: 238 1554 2038 2723 238
Size: 1316 484 685 2506
Fragments arranged by size:
2506 1316 685 484
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4991
PvuII CAG'CTG
Cuts at: 1843 2416 2776 1843
Size: 573 360 4058
Fragments arranged by size:
4058 573 360
RsrII CG'GwC_CG
Cuts at: 3186 3186
Size: 4991
SacI G_AGCT'C
Cuts at: 2005 2005
Size: 4991
SalI G'TCGA_C
Cuts at: 2028 2028
Size: 4991
SanDI GG'GwC_CC
Cuts at: 479 479
Size: 4991
SapI GCTCTTCn'nnn_
Cuts at: 3014 3224 3014
Size: 210 4781
SbfI CC_TGCA'GG
Cuts at: 2038 2038
Size: 4991
ScaI AGT'ACT
Cuts at: 4508 4508
Size: 4991
SfcI C'TryA_G
Cuts at: 33 234 830 1550 1806 2034 2719 3712 3903
Size: 201 596 720 256 228 685 993 191
Cuts at: 3903 33
Size: 1121
Fragments arranged by size:
1121 993 720 685 596 256 228 201
191
SfoI GGC'GCC
Cuts at: 2423 2671 2423
Size: 248 4743
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4991
SmaI CCC'GGG
Cuts at: 2013 2013
Size: 4991
SmlI C'TyrA_G
Cuts at: 694 1337 2146 3061 3553 3815 694
Size: 643 809 915 492 262 1870
Fragments arranged by size:
1870 915 809 643 492 262
SpeI A'CTAG_T
Cuts at: 67 1912 67
Size: 1845 3146
SphI G_CATG'C
Cuts at: 1489 2044 2544 1489
Size: 555 500 3936
Fragments arranged by size:
3936 555 500
SspI AAT'ATT
Cuts at: 870 870
Size: 4991
StuI AGG'CCT
Cuts at: 778 778
Size: 4991
StyI C'CwwG_G
Cuts at: 483 1511 1595 2125 2479 483
Size: 1028 84 530 354 2995
Fragments arranged by size:
2995 1028 530 354 84
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 357 367 2574 2707 3166 3375 357
Size: 10 2207 133 459 209 1973
Fragments arranged by size:
2207 1973 459 209 133 10
TatI w'GTAC_w
Cuts at: 1447 4506 1447
Size: 3059 1932
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 766 798 887 919 1899 1931 4508 4540 766
Size: 32 89 32 980 32 2577 32 1217
Fragments arranged by size:
2577 1217 980 89 32 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 2788 2788
Size: 4991
XbaI T'CTAG_A
Cuts at: 2022 2022
Size: 4991
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1734 2486 1734
Size: 752 4239
XmaI C'CCGG_G
Cuts at: 2011 2011
Size: 4991
Enzymes that do cut:
AarI AccI Acc65I AclI AcuI AflIII AgeI AlwNI
ApaLI ApoI AseI AsiSI AvaI AvrII BamHI BanI
BanII BbeI BbsI BciVI BclI BglI BglII BlpI
Bme1580I BmgBI BmrI BplI BpmI Bpu10I BpuEI BsaI
BsaAI BsaBI BsaHI BsaWI BsaXI BseYI BsgI BsiEI
BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI
BsrFI BssSI BstAPI BstBI BstYI Bsu36I BtgI BtgZI
BtsI DraI DraIII DrdI EaeI EagI EarI EciI
EcoICRI Eco57MI EcoNI EcoO109I EcoRI EcoRV FalI FspI
HaeII Hin4I HincII KasI KpnI MfeI MluI MmeI
MscI MslI MspA1I NaeI NarI NdeI NgoMIV NspI
PciI PflMI PmeI PmlI PpiI PpuMI PstI PvuI
PvuII RsrII SacI SalI SanDI SapI SbfI ScaI
SfcI SfoI SgfI SmaI SmlI SpeI SphI SspI
StuI StyI TaqII TatI TstI Tth111I XbaI XcmI
XmaI
Enzymes that do not cut:
AatII AfeI AflII AhdI AleI AlfI AloI ApaI
AscI BaeI BbvCI BcgI BfrBI BmtI BseRI BsiWI
BsmBI BsrGI BssHII BstEII BstXI BstZ17I ClaI CspCI
FseI FspAI HindIII HpaI NcoI NheI NotI NruI
NsiI PacI PasI PfoI PshAI PsiI PspOMI PspXI
PsrI SacII SexAI SfiI SgrAI SnaBI SrfI SwaI
XhoI XmnI ZraI