Restriction map for
pF1KE0323
(Circular) (Six-base) MAPSORT of: pF1KE0323.seq Check: 9343 from: 1 to: 5645
pF1KE0323 5645 bp
With 182 enzymes: SgfI *
April 24, 2009 13:13 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2446 2446
Size: 5645
AccI GT'mk_AC
Cuts at: 1214 2683 1214
Size: 1469 4176
Acc65I G'GTAC_C
Cuts at: 2661 2661
Size: 5645
AclI AA'CG_TT
Cuts at: 5485 5485
Size: 5645
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 259 430 700 1428 2435 3470 3902 4649 4917
Size: 171 270 728 1007 1035 432 747 268
Cuts at: 4917 259
Size: 987
Fragments arranged by size:
1035 1007 987 747 728 432 270 268
171
AfeI AGC'GCT
Cuts at: 805 805
Size: 5645
AflIII A'CryG_T
Cuts at: 2997 4101 5078 2997
Size: 1104 977 3564
Fragments arranged by size:
3564 1104 977
AgeI A'CCGG_T
Cuts at: 4033 4033
Size: 5645
AhdI GACnn_n'nnGTC
Cuts at: 1221 2007 1221
Size: 786 4859
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 741 2083 741
Size: 1342 4303
AlwNI CAG_nnn'CTG
Cuts at: 736 2458 2950 4517 736
Size: 1722 492 1567 1864
Fragments arranged by size:
1864 1722 1567 492
ApaI G_GGCC'C
Cuts at: 1419 1419
Size: 5645
ApaLI G'TGCA_C
Cuts at: 4415 4415
Size: 5645
ApoI r'AATT_y
Cuts at: 2649 4998 5254 2649
Size: 2349 256 3040
Fragments arranged by size:
3040 2349 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 5645
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 5645
AvaI C'yCGr_G
Cuts at: 1527 1589 1727 2665 1527
Size: 62 138 938 4507
Fragments arranged by size:
4507 938 138 62
AvrII C'CTAG_G
Cuts at: 2353 2478 2353
Size: 125 5520
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 244 277 244
Size: 33 5612
BamHI G'GATC_C
Cuts at: 2090 2670 2090
Size: 580 5065
BanI G'GyrC_C
Cuts at: 572 662 1786 2661 3075 3323 3358 572
Size: 90 1124 875 414 248 35 2859
Fragments arranged by size:
2859 1124 875 414 248 90 35
BanII G_rGCy'C
Cuts at: 611 1157 1307 1351 1419 1465 1735 2659 3689
Size: 546 150 44 68 46 270 924 1030
Cuts at: 3689 611
Size: 2567
Fragments arranged by size:
2567 1030 924 546 270 150 68 46
44
BbeI G_GCGC'C
Cuts at: 3079 3327 3079
Size: 248 5397
BbsI GAAGACnn'nnnn_
Cuts at: 145 831 1055 1523 145
Size: 686 224 468 4267
Fragments arranged by size:
4267 686 468 224
BbvCI CC'TCA_GC
Cuts at: 737 1363 1831 737
Size: 626 468 4551
Fragments arranged by size:
4551 626 468
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1708 1742 1708
Size: 34 5611
BciVI GTATCCnnnnn_n'
Cuts at: 3543 4304 5645 3543
Size: 761 1341 3543
Fragments arranged by size:
3543 1341 761
BclI T'GATC_A
Cuts at: 3165 3165
Size: 5645
BfrBI ATG'CAT
Cuts at: 2025 2025
Size: 5645
BglII A'GATC_T
Cuts at: 1540 3160 5197 1540
Size: 1620 2037 1988
Fragments arranged by size:
2037 1988 1620
BlpI GC'TnA_GC
Cuts at: 1243 1867 1978 2757 1243
Size: 624 111 779 4131
Fragments arranged by size:
4131 779 624 111
Bme1580I G_kGCm'C
Cuts at: 885 1419 1882 2353 3270 3363 4419 885
Size: 534 463 471 917 93 1056 2111
Fragments arranged by size:
2111 1056 917 534 471 463 93
BmrI ACTGGGnnnn_n'
Cuts at: 466 2857 2938 3273 5417 466
Size: 2391 81 335 2144 694
Fragments arranged by size:
2391 2144 694 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2247 4012 2247
Size: 1765 3880
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 148 329 1308 148
Size: 181 979 4485
Fragments arranged by size:
4485 979 181
Bpu10I CC'TnA_GC
Cuts at: 737 1363 1831 737
Size: 626 468 4551
Fragments arranged by size:
4551 626 468
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2821 3700 4192 4490 2821
Size: 879 492 298 3976
Fragments arranged by size:
3976 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 940 1579 1994 5349 940
Size: 639 415 3355 1236
Fragments arranged by size:
3355 1236 639 415
BsaAI yAC'GTr
Cuts at: 2998 2998
Size: 5645
BsaBI GATnn'nnATC
Cuts at: 6 3159 5196 6
Size: 3153 2037 455
Fragments arranged by size:
3153 2037 455
BsaHI Gr'CG_yC
Cuts at: 1708 3076 3324 4026 5522 1708
Size: 1368 248 702 1496 1831
Fragments arranged by size:
1831 1496 1368 702 248
BsaWI w'CCGG_w
Cuts at: 1904 2834 3355 4033 4307 4454 4865 1904
Size: 930 521 678 274 147 411 2684
Fragments arranged by size:
2684 930 678 521 411 274 147
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1059 1165 1305 1351 1633 2433 2436 1059
Size: 106 140 46 282 800 3 4268
Fragments arranged by size:
4268 800 282 140 106 46 3
BseYI C'CCAG_C
Cuts at: 792 1042 1264 1411 1418 1940 2399 2495 3070
Size: 250 222 147 7 522 459 96 575
Cuts at: 3070 3828 4405 4901 792
Size: 758 577 496 1536
Fragments arranged by size:
1536 758 577 575 522 496 459 250
222 147 96 7
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 2521 2521
Size: 5645
BsiEI CG_ry'CG
Cuts at: 104 3187 3233 4441 104
Size: 3083 46 1208 1308
Fragments arranged by size:
3083 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 611 966 1465 1805 2659 3437 3627 4419 611
Size: 355 499 340 854 778 190 792 1837
Fragments arranged by size:
1837 854 792 778 499 355 340 190
BsiWI C'GTAC_G
Cuts at: 588 588
Size: 5645
BsmI GAATG_Cn'
Cuts at: 730 1513 2388 730
Size: 783 875 3987
Fragments arranged by size:
3987 875 783
Bsp1286I G_dGCh'C
Cuts at: 611 885 966 1157 1307 1351 1419 1465 1735
Size: 274 81 191 150 44 68 46 270
Cuts at: 1735 1805 1882 2353 2659 3270 3363 3437 3627
Size: 70 77 471 306 611 93 74 190
Cuts at: 3627 3689 4419 611
Size: 62 730 1837
Fragments arranged by size:
1837 730 611 471 306 274 270 191
190 150 93 81 77 74 70 68
62 46 44
BspEI T'CCGG_A
Cuts at: 1904 4865 1904
Size: 2961 2684
BspHI T'CATG_A
Cuts at: 812 4821 812
Size: 4009 1636
BspMI ACCTGCnnnn'nnnn_
Cuts at: 2012 2446 2695 2909 3211 3592 2012
Size: 434 249 214 302 381 4065
Fragments arranged by size:
4065 434 381 302 249 214
BsrBI CCG'CTC
Cuts at: 50 3937 3991 5475 5640 50
Size: 3887 54 1484 165 55
Fragments arranged by size:
3887 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 609 3557 609
Size: 2948 2697
BsrFI r'CCGG_y
Cuts at: 1196 3643 3824 4033 1196
Size: 2447 181 209 2808
Fragments arranged by size:
2808 2447 209 181
BssHII G'CGCG_C
Cuts at: 2503 2503
Size: 5645
BssSI C'ACGA_G
Cuts at: 3916 4274 3916
Size: 358 5287
BstAPI GCAn_nnn'nTGC
Cuts at: 599 3022 599
Size: 2423 3222
BstBI TT'CG_AA
Cuts at: 4006 4006
Size: 5645
BstXI CCAn_nnnn'nTGG
Cuts at: 520 520
Size: 5645
BstYI r'GATC_y
Cuts at: 1 618 1540 2090 2670 3137 3160 3495 3741
Size: 617 922 550 580 467 23 335 246
Cuts at: 3741 4753 4839 4851 5197 1
Size: 1012 86 12 346 449
Fragments arranged by size:
1012 922 617 580 550 467 449 346
335 246 86 23 12
BtgI C'CryG_G
Cuts at: 107 450 558 1736 2013 107
Size: 343 108 1178 277 3739
Fragments arranged by size:
3739 1178 343 277 108
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 516 716 2540 3595 3776 3939 516
Size: 200 1824 1055 181 163 2222
Fragments arranged by size:
2222 1824 1055 200 181 163
BtsI GCAGTG_nn'
Cuts at: 840 966 1044 1122 1225 1663 2888 840
Size: 126 78 78 103 438 1225 3597
Fragments arranged by size:
3597 1225 438 126 103 78 78
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 547 582 547
Size: 35 5610
DraI TTT'AAA
Cuts at: 2644 2644
Size: 5645
DrdI GACnn_nn'nnGTC
Cuts at: 3351 4209 3351
Size: 858 4787
EaeI y'GGCC_r
Cuts at: 555 798 924 991 1472 2210 2343 3230 3404
Size: 243 126 67 481 738 133 887 174
Cuts at: 3404 3795 3822 5167 555
Size: 391 27 1345 1033
Fragments arranged by size:
1345 1033 887 738 481 391 243 174
133 126 67 27
EagI C'GGCC_G
Cuts at: 3230 3230
Size: 5645
EarI CTCTTCn'nnn_
Cuts at: 206 275 389 394 1295 1619 1625 2115 2454
Size: 69 114 5 901 324 6 490 339
Cuts at: 2454 2611 3668 3878 206
Size: 157 1057 210 1973
Fragments arranged by size:
1973 1057 901 490 339 324 210 157
114 69 6 5
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 1068 3853 4163 4309 5455 1068
Size: 2785 310 146 1146 1258
Fragments arranged by size:
2785 1258 1146 310 146
EcoICRI GAG'CTC
Cuts at: 609 1463 2657 609
Size: 854 1194 3597
Fragments arranged by size:
3597 1194 854
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 148 259 329 430 700 1308 1428 2435 3470
Size: 111 70 101 270 608 120 1007 1035
Cuts at: 3470 3902 4649 4917 148
Size: 432 747 268 876
Fragments arranged by size:
1035 1007 876 747 608 432 270 268
120 111 101 70
EcoNI CCTnn'n_nnAGG
Cuts at: 388 388
Size: 5645
EcoO109I rG'GnC_Cy
Cuts at: 225 1278 1415 2249 2324 2334 2404 2784 225
Size: 1053 137 834 75 10 70 380 3086
Fragments arranged by size:
3086 1053 834 380 137 75 70 10
EcoRI G'AATT_C
Cuts at: 2649 2649
Size: 5645
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2144 3664 2144
Size: 1520 4125
FspI TGC'GCA
Cuts at: 3426 3426
Size: 5645
HaeII r_GCGC'y
Cuts at: 807 1797 3079 3327 4349 5328 807
Size: 990 1282 248 1022 979 1124
Fragments arranged by size:
1282 1124 1022 990 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 178 210 500 532 1207 1239 1583 1615 1656
Size: 32 290 32 675 32 344 32 41
Cuts at: 1656 1688 1792 1824 1993 2025 2474 2506 3487
Size: 32 104 32 169 32 449 32 981
Cuts at: 3487 3517 3519 3549 178
Size: 30 2 30 2274
Fragments arranged by size:
2274 981 675 449 344 290 169 104
41 32 32 32 32 32 32 32
32 30 30 2
HincII GTy'rAC
Cuts at: 300 2684 300
Size: 2384 3261
HindIII A'AGCT_T
Cuts at: 412 412
Size: 5645
KasI G'GCGC_C
Cuts at: 3075 3323 3075
Size: 248 5397
KpnI G_GTAC'C
Cuts at: 2665 2665
Size: 5645
MluI A'CGCG_T
Cuts at: 5078 5078
Size: 5645
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1642 2178 2216 2345 4316 4500 1642
Size: 536 38 129 1971 184 2787
Fragments arranged by size:
2787 1971 536 184 129 38
MscI TGG'CCA
Cuts at: 557 1474 2212 3406 557
Size: 917 738 1194 2796
Fragments arranged by size:
2796 1194 917 738
MslI CAynn'nnrTG
Cuts at: 359 535 935 3634 3761 359
Size: 176 400 2699 127 2243
Fragments arranged by size:
2699 2243 400 176 127
MspA1I CmG'CkG
Cuts at: 126 345 452 1179 2295 2399 2756 3070 3430
Size: 219 107 727 1116 104 357 314 360
Cuts at: 3430 4443 4688 126
Size: 1013 245 1083
Fragments arranged by size:
1116 1083 1013 727 360 357 314 245
219 107 104
NaeI GCC'GGC
Cuts at: 3826 3826
Size: 5645
NarI GG'CG_CC
Cuts at: 3076 3324 3076
Size: 248 5397
NcoI C'CATG_G
Cuts at: 107 1736 107
Size: 1629 4016
NgoMIV G'CCGG_C
Cuts at: 3824 3824
Size: 5645
NsiI A_TGCA'T
Cuts at: 2027 2027
Size: 5645
NspI r_CATG'y
Cuts at: 940 1242 1489 2698 3198 4105 940
Size: 302 247 1209 500 907 2480
Fragments arranged by size:
2480 1209 907 500 302 247
PciI A'CATG_T
Cuts at: 4101 4101
Size: 5645
PflMI CCAn_nnn'nTGG
Cuts at: 1956 1956
Size: 5645
PfoI T'CCnGG_A
Cuts at: 306 1395 2303 2326 306
Size: 1089 908 23 3625
Fragments arranged by size:
3625 1089 908 23
PmeI GTTT'AAAC
Cuts at: 2644 2644
Size: 5645
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 166 198 4784 4816 166
Size: 32 4586 32 995
Fragments arranged by size:
4586 995 32 32
PpuMI rG'GwC_Cy
Cuts at: 225 1278 2324 2334 2404 225
Size: 1053 1046 10 70 3466
Fragments arranged by size:
3466 1053 1046 70 10
PshAI GACnn'nnGTC
Cuts at: 905 905
Size: 5645
PspOMI G'GGCC_C
Cuts at: 1415 1415
Size: 5645
PstI C_TGCA'G
Cuts at: 1039 1232 1358 2009 2692 3377 1039
Size: 193 126 651 683 685 3307
Fragments arranged by size:
3307 685 683 651 193 126
PvuI CG_AT'CG
Cuts at: 104 104
Size: 5645
PvuII CAG'CTG
Cuts at: 345 1179 3070 3430 345
Size: 834 1891 360 2560
Fragments arranged by size:
2560 1891 834 360
RsrII CG'GwC_CG
Cuts at: 3840 3840
Size: 5645
SacI G_AGCT'C
Cuts at: 611 1465 2659 611
Size: 854 1194 3597
Fragments arranged by size:
3597 1194 854
SacII CC_GC'GG
Cuts at: 453 453
Size: 5645
SalI G'TCGA_C
Cuts at: 2682 2682
Size: 5645
SanDI GG'GwC_CC
Cuts at: 225 1278 2324 2404 225
Size: 1053 1046 80 3466
Fragments arranged by size:
3466 1053 1046 80
SapI GCTCTTCn'nnn_
Cuts at: 1295 2115 2611 3668 3878 1295
Size: 820 496 1057 210 3062
Fragments arranged by size:
3062 1057 820 496 210
SbfI CC_TGCA'GG
Cuts at: 2692 2692
Size: 5645
ScaI AGT'ACT
Cuts at: 262 5162 262
Size: 4900 745
SfcI C'TryA_G
Cuts at: 33 264 1035 1228 1354 1389 1554 1995 2005
Size: 231 771 193 126 35 165 441 10
Cuts at: 2005 2688 3373 4366 4557 33
Size: 683 685 993 191 1121
Fragments arranged by size:
1121 993 771 685 683 441 231 193
191 165 126 35 10
SfoI GGC'GCC
Cuts at: 3077 3325 3077
Size: 248 5397
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 5645
SmaI CCC'GGG
Cuts at: 1529 2667 1529
Size: 1138 4507
SmlI C'TyrA_G
Cuts at: 2800 3715 4207 4469 2800
Size: 915 492 262 3976
Fragments arranged by size:
3976 915 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 5645
SphI G_CATG'C
Cuts at: 940 2698 3198 940
Size: 1758 500 3387
Fragments arranged by size:
3387 1758 500
SspI AAT'ATT
Cuts at: 868 868
Size: 5645
StuI AGG'CCT
Cuts at: 465 1769 465
Size: 1304 4341
StyI C'CwwG_G
Cuts at: 107 903 1100 1736 2353 2478 2779 3133 107
Size: 796 197 636 617 125 301 354 2619
Fragments arranged by size:
2619 796 636 617 354 301 197 125
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1278 3228 3361 3820 4029 1278
Size: 1950 133 459 209 2894
Fragments arranged by size:
2894 1950 459 209 133
TatI w'GTAC_w
Cuts at: 260 5160 260
Size: 4900 745
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 1838 1870 5162 5194 1838
Size: 32 3292 32 2289
Fragments arranged by size:
3292 2289 32 32
Tth111I GACn'n_nGTC
Cuts at: 2361 3442 2361
Size: 1081 4564
XbaI T'CTAG_A
Cuts at: 2676 2676
Size: 5645
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 520 1743 3140 520
Size: 1223 1397 3025
Fragments arranged by size:
3025 1397 1223
XmaI C'CCGG_G
Cuts at: 1527 2665 1527
Size: 1138 4507
XmnI GAAnn'nnTTC
Cuts at: 415 415
Size: 5645
Enzymes that do cut:
AarI AccI Acc65I AclI AcuI AfeI AflIII AgeI
AhdI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI
AvaI AvrII BaeI BamHI BanI BanII BbeI BbsI
BbvCI BcgI BciVI BclI BfrBI BglII BlpI Bme1580I
BmrI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BseRI BseYI BsgI BsiEI BsiHKAI BsiWI
BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BssHII BssSI BstAPI BstBI BstXI BstYI BtgI BtgZI
BtsI CspCI DraI DrdI EaeI EagI EarI EciI
EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI FspI HaeII
Hin4I HincII HindIII KasI KpnI MluI MmeI MscI
MslI MspA1I NaeI NarI NcoI NgoMIV NsiI NspI
PciI PflMI PfoI PmeI PpiI PpuMI PshAI PspOMI
PstI PvuI PvuII RsrII SacI SacII SalI SanDI
SapI SbfI ScaI SfcI SfoI SgfI SmaI SmlI
SpeI SphI SspI StuI StyI TaqII TatI TstI
Tth111I XbaI XcmI XmaI XmnI
Enzymes that do not cut:
AatII AflII AleI AloI AscI BglI BmgBI BmtI
BsaXI BsmBI BsrGI BstEII BstZ17I Bsu36I ClaI DraIII
EcoRV FseI FspAI HpaI MfeI NdeI NheI NotI
NruI PacI PasI PmlI PsiI PspXI PsrI SexAI
SfiI SgrAI SnaBI SrfI SwaI XhoI ZraI