Restriction map for
pF1KE0308
(Circular) (Six-base) MAPSORT of: pF1KE0308.seq Check: 8203 from: 1 to: 4670
pF1KE0308 4670 bp
With 182 enzymes: SgfI *
April 24, 2009 13:12 ..
AccI GT'mk_AC
Cuts at: 1563 1708 1563
Size: 145 4525
Acc65I G'GTAC_C
Cuts at: 370 1686 370
Size: 1316 3354
AclI AA'CG_TT
Cuts at: 4510 4510
Size: 4670
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 541 1046 1501 2495 2927 3674 3942 541
Size: 505 455 994 432 747 268 1269
Fragments arranged by size:
1269 994 747 505 455 432 268
AflIII A'CryG_T
Cuts at: 2022 3126 4103 2022
Size: 1104 977 2589
Fragments arranged by size:
2589 1104 977
AgeI A'CCGG_T
Cuts at: 3058 3058
Size: 4670
AlwNI CAG_nnn'CTG
Cuts at: 1122 1471 1480 1524 1975 3542 1122
Size: 349 9 44 451 1567 2250
Fragments arranged by size:
2250 1567 451 349 44 9
ApaI G_GGCC'C
Cuts at: 685 685
Size: 4670
ApaLI G'TGCA_C
Cuts at: 3440 3440
Size: 4670
ApoI r'AATT_y
Cuts at: 1610 1674 4023 4279 1610
Size: 64 2349 256 2001
Fragments arranged by size:
2349 2001 256 64
AseI AT'TA_AT
Cuts at: 20 20
Size: 4670
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4670
AvaI C'yCGr_G
Cuts at: 1690 1690
Size: 4670
BamHI G'GATC_C
Cuts at: 959 1695 959
Size: 736 3934
BanI G'GyrC_C
Cuts at: 370 1686 2100 2348 2383 370
Size: 1316 414 248 35 2657
Fragments arranged by size:
2657 1316 414 248 35
BanII G_rGCy'C
Cuts at: 685 812 840 971 1684 2714 685
Size: 127 28 131 713 1030 2641
Fragments arranged by size:
2641 1030 713 131 127 28
BbeI G_GCGC'C
Cuts at: 2104 2352 2104
Size: 248 4422
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 497 531 497
Size: 34 4636
BciVI GTATCCnnnnn_n'
Cuts at: 2568 3329 4670 2568
Size: 761 1341 2568
Fragments arranged by size:
2568 1341 761
BclI T'GATC_A
Cuts at: 2190 2190
Size: 4670
BfrBI ATG'CAT
Cuts at: 1051 1051
Size: 4670
BglII A'GATC_T
Cuts at: 2185 4222 2185
Size: 2037 2633
BlpI GC'TnA_GC
Cuts at: 754 1782 754
Size: 1028 3642
Bme1580I G_kGCm'C
Cuts at: 232 685 2295 2388 3444 232
Size: 453 1610 93 1056 1458
Fragments arranged by size:
1610 1458 1056 453 93
BmrI ACTGGGnnnn_n'
Cuts at: 1882 1963 2298 4442 1882
Size: 81 335 2144 2110
Fragments arranged by size:
2144 2110 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 299 3037 299
Size: 2738 1932
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 207 271 368 666 690 874 207
Size: 64 97 298 24 184 4003
Fragments arranged by size:
4003 298 184 97 64 24
Bpu10I CC'TnA_GC
Cuts at: 608 608
Size: 4670
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 895 1846 2725 3217 3515 895
Size: 951 879 492 298 2050
Fragments arranged by size:
2050 951 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 4374 4374
Size: 4670
BsaAI yAC'GTr
Cuts at: 2023 2023
Size: 4670
BsaBI GATnn'nnATC
Cuts at: 6 2184 4221 6
Size: 2178 2037 455
Fragments arranged by size:
2178 2037 455
BsaHI Gr'CG_yC
Cuts at: 198 2101 2349 3051 4547 198
Size: 1903 248 702 1496 321
Fragments arranged by size:
1903 1496 702 321 248
BsaWI w'CCGG_w
Cuts at: 1859 2380 3058 3332 3479 3890 1859
Size: 521 678 274 147 411 2639
Fragments arranged by size:
2639 678 521 411 274 147
BseYI C'CCAG_C
Cuts at: 225 700 2095 2853 3430 3926 225
Size: 475 1395 758 577 496 969
Fragments arranged by size:
1395 969 758 577 496 475
BsiEI CG_ry'CG
Cuts at: 104 2212 2258 3466 104
Size: 2108 46 1208 1308
Fragments arranged by size:
2108 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 881 1684 2462 2652 3444 881
Size: 803 778 190 792 2107
Fragments arranged by size:
2107 803 792 778 190
BsmI GAATG_Cn'
Cuts at: 782 782
Size: 4670
Bsp1286I G_dGCh'C
Cuts at: 232 685 812 840 881 971 1684 2295 2388
Size: 453 127 28 41 90 713 611 93
Cuts at: 2388 2462 2652 2714 3444 232
Size: 74 190 62 730 1458
Fragments arranged by size:
1458 730 713 611 453 190 127 93
90 74 62 41 28
BspEI T'CCGG_A
Cuts at: 3890 3890
Size: 4670
BspHI T'CATG_A
Cuts at: 1191 3846 1191
Size: 2655 2015
BspMI ACCTGCnnnn'nnnn_
Cuts at: 1720 1934 2236 2617 1720
Size: 214 302 381 3773
Fragments arranged by size:
3773 381 302 214
BsrBI CCG'CTC
Cuts at: 50 833 2962 3016 4500 4665 50
Size: 783 2129 54 1484 165 55
Fragments arranged by size:
2129 1484 783 165 55 54
BsrDI GCAATG_nn'
Cuts at: 405 2582 405
Size: 2177 2493
BsrFI r'CCGG_y
Cuts at: 194 459 2668 2849 3058 194
Size: 265 2209 181 209 1806
Fragments arranged by size:
2209 1806 265 209 181
BsrGI T'GTAC_A
Cuts at: 428 1293 428
Size: 865 3805
BssSI C'ACGA_G
Cuts at: 2941 3299 2941
Size: 358 4312
BstAPI GCAn_nnn'nTGC
Cuts at: 520 2047 520
Size: 1527 3143
BstBI TT'CG_AA
Cuts at: 3031 3031
Size: 4670
BstXI CCAn_nnnn'nTGG
Cuts at: 249 400 249
Size: 151 4519
BstYI r'GATC_y
Cuts at: 1 725 959 1695 2162 2185 2520 2766 3778
Size: 724 234 736 467 23 335 246 1012
Cuts at: 3778 3864 3876 4222 1
Size: 86 12 346 449
Fragments arranged by size:
1012 736 724 467 449 346 335 246
234 86 23 12
BtgI C'CryG_G
Cuts at: 169 471 169
Size: 302 4368
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 304 2620 2801 2964 304
Size: 2316 181 163 2010
Fragments arranged by size:
2316 2010 181 163
BtsI GCAGTG_nn'
Cuts at: 230 515 806 1913 230
Size: 285 291 1107 2987
Fragments arranged by size:
2987 1107 291 285
ClaI AT'CG_AT
Cuts at: 1586 1586
Size: 4670
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 453 488 453
Size: 35 4635
DraI TTT'AAA
Cuts at: 1669 1669
Size: 4670
DraIII CAC_nnn'GTG
Cuts at: 236 236
Size: 4670
DrdI GACnn_nn'nnGTC
Cuts at: 984 2376 3234 984
Size: 1392 858 2420
Fragments arranged by size:
2420 1392 858
EaeI y'GGCC_r
Cuts at: 461 1020 1146 2255 2429 2820 2847 4192 461
Size: 559 126 1109 174 391 27 1345 939
Fragments arranged by size:
1345 1109 939 559 391 174 126 27
EagI C'GGCC_G
Cuts at: 2255 2255
Size: 4670
EarI CTCTTCn'nnn_
Cuts at: 1483 2693 2903 1483
Size: 1210 210 3250
Fragments arranged by size:
3250 1210 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 805 2878 3188 3334 4480 805
Size: 2073 310 146 1146 995
Fragments arranged by size:
2073 1146 995 310 146
EcoICRI GAG'CTC
Cuts at: 1682 1682
Size: 4670
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 207 271 368 541 666 690 874 1046 1501
Size: 64 97 173 125 24 184 172 455
Cuts at: 1501 2495 2927 3674 3942 207
Size: 994 432 747 268 935
Fragments arranged by size:
994 935 747 455 432 268 184 173
172 125 97 64 24
EcoNI CCTnn'n_nnAGG
Cuts at: 1166 1166
Size: 4670
EcoO109I rG'GnC_Cy
Cuts at: 206 278 681 977 1809 206
Size: 72 403 296 832 3067
Fragments arranged by size:
3067 832 403 296 72
EcoRI G'AATT_C
Cuts at: 1674 1674
Size: 4670
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 936 1422 2689 936
Size: 486 1267 2917
Fragments arranged by size:
2917 1267 486
FspI TGC'GCA
Cuts at: 2451 2451
Size: 4670
HaeII r_GCGC'y
Cuts at: 743 875 2104 2352 3374 4353 743
Size: 132 1229 248 1022 979 1060
Fragments arranged by size:
1229 1060 1022 979 248 132
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1224 1256 2512 2542 2544 2574 1224
Size: 32 1256 30 2 30 3320
Fragments arranged by size:
3320 1256 32 30 30 2
HincII GTy'rAC
Cuts at: 1709 1709
Size: 4670
HindIII A'AGCT_T
Cuts at: 1579 1579
Size: 4670
KasI G'GCGC_C
Cuts at: 2100 2348 2100
Size: 248 4422
KpnI G_GTAC'C
Cuts at: 374 1690 374
Size: 1316 3354
MluI A'CGCG_T
Cuts at: 4103 4103
Size: 4670
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 525 3341 3525 525
Size: 2816 184 1670
Fragments arranged by size:
2816 1670 184
MscI TGG'CCA
Cuts at: 2431 2431
Size: 4670
MslI CAynn'nnrTG
Cuts at: 1190 2659 2786 1190
Size: 1469 127 3074
Fragments arranged by size:
3074 1469 127
MspA1I CmG'CkG
Cuts at: 171 884 1781 2095 2455 3468 3713 171
Size: 713 897 314 360 1013 245 1128
Fragments arranged by size:
1128 1013 897 713 360 314 245
NaeI GCC'GGC
Cuts at: 196 461 2851 196
Size: 265 2390 2015
Fragments arranged by size:
2390 2015 265
NarI GG'CG_CC
Cuts at: 2101 2349 2101
Size: 248 4422
NgoMIV G'CCGG_C
Cuts at: 194 459 2849 194
Size: 265 2390 2015
Fragments arranged by size:
2390 2015 265
NsiI A_TGCA'T
Cuts at: 1053 1053
Size: 4670
NspI r_CATG'y
Cuts at: 508 1723 2223 3130 508
Size: 1215 500 907 2048
Fragments arranged by size:
2048 1215 907 500
PasI CC'CwG_GG
Cuts at: 393 526 393
Size: 133 4537
PciI A'CATG_T
Cuts at: 3126 3126
Size: 4670
PfoI T'CCnGG_A
Cuts at: 663 663
Size: 4670
PmeI GTTT'AAAC
Cuts at: 1669 1669
Size: 4670
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3809 3841 3809
Size: 32 4638
PpuMI rG'GwC_Cy
Cuts at: 206 977 206
Size: 771 3899
PsiI TTA'TAA
Cuts at: 1238 1238
Size: 4670
PspOMI G'GGCC_C
Cuts at: 681 681
Size: 4670
PstI C_TGCA'G
Cuts at: 416 698 1476 1717 2402 416
Size: 282 778 241 685 2684
Fragments arranged by size:
2684 778 685 282 241
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4670
PvuII CAG'CTG
Cuts at: 2095 2455 2095
Size: 360 4310
RsrII CG'GwC_CG
Cuts at: 2865 2865
Size: 4670
SacI G_AGCT'C
Cuts at: 1684 1684
Size: 4670
SacII CC_GC'GG
Cuts at: 172 172
Size: 4670
SalI G'TCGA_C
Cuts at: 1707 1707
Size: 4670
SapI GCTCTTCn'nnn_
Cuts at: 1483 2693 2903 1483
Size: 1210 210 3250
Fragments arranged by size:
3250 1210 210
SbfI CC_TGCA'GG
Cuts at: 1717 1717
Size: 4670
ScaI AGT'ACT
Cuts at: 4187 4187
Size: 4670
SexAI A'CCwGG_T
Cuts at: 620 620
Size: 4670
SfcI C'TryA_G
Cuts at: 33 412 590 694 729 1472 1713 2398 3391
Size: 379 178 104 35 743 241 685 993
Cuts at: 3391 3582 33
Size: 191 1121
Fragments arranged by size:
1121 993 743 685 379 241 191 178
104 35
SfoI GGC'GCC
Cuts at: 2102 2350 2102
Size: 248 4422
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4670
SmaI CCC'GGG
Cuts at: 1692 1692
Size: 4670
SmlI C'TyrA_G
Cuts at: 910 1825 2740 3232 3494 910
Size: 915 915 492 262 2086
Fragments arranged by size:
2086 915 915 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4670
SphI G_CATG'C
Cuts at: 508 1723 2223 508
Size: 1215 500 2955
Fragments arranged by size:
2955 1215 500
StyI C'CwwG_G
Cuts at: 202 920 1326 1372 1804 2158 202
Size: 718 406 46 432 354 2714
Fragments arranged by size:
2714 718 432 406 354 46
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1093 2253 2386 2845 3054 1093
Size: 1160 133 459 209 2709
Fragments arranged by size:
2709 1160 459 209 133
TatI w'GTAC_w
Cuts at: 428 1174 1293 4185 428
Size: 746 119 2892 913
Fragments arranged by size:
2892 913 746 119
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 1337 1369 4187 4219 1337
Size: 32 2818 32 1788
Fragments arranged by size:
2818 1788 32 32
Tth111I GACn'n_nGTC
Cuts at: 2467 2467
Size: 4670
XbaI T'CTAG_A
Cuts at: 1701 1701
Size: 4670
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2165 2165
Size: 4670
XmaI C'CCGG_G
Cuts at: 1690 1690
Size: 4670
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AgeI AlwNI ApaI
ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII
BbeI BcgI BciVI BclI BfrBI BglII BlpI Bme1580I
BmrI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BseYI BsiEI BsiHKAI BsmI Bsp1286I BspEI
BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssSI BstAPI
BstBI BstXI BstYI BtgI BtgZI BtsI ClaI CspCI
DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI
Eco57MI EcoNI EcoO109I EcoRI FalI FspI HaeII Hin4I
HincII HindIII KasI KpnI MluI MmeI MscI MslI
MspA1I NaeI NarI NgoMIV NsiI NspI PasI PciI
PfoI PmeI PpiI PpuMI PsiI PspOMI PstI PvuI
PvuII RsrII SacI SacII SalI SapI SbfI ScaI
SexAI SfcI SfoI SgfI SmaI SmlI SpeI SphI
StyI TaqII TatI TstI Tth111I XbaI XcmI XmaI
Enzymes that do not cut:
AarI AatII AfeI AflII AhdI AleI AlfI AloI
AscI AvrII BaeI BbsI BbvCI BglI BmgBI BmtI
BsaXI BseRI BsgI BsiWI BsmBI BssHII BstEII BstZ17I
Bsu36I EcoRV FseI FspAI HpaI MfeI NcoI NdeI
NheI NotI NruI PacI PflMI PmlI PshAI PspXI
PsrI SanDI SfiI SgrAI SnaBI SrfI SspI StuI
SwaI XhoI XmnI ZraI