Restriction map for
pF1KE0303
(Circular) (Six-base) MAPSORT of: pF1KE0303.seq Check: 5464 from: 1 to: 4901
pF1KE0303 4901 bp
With 182 enzymes: SgfI *
April 24, 2009 13:12 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 162 162
Size: 4901
AccI GT'mk_AC
Cuts at: 1939 1939
Size: 4901
Acc65I G'GTAC_C
Cuts at: 1917 1917
Size: 4901
AclI AA'CG_TT
Cuts at: 1895 4741 1895
Size: 2846 2055
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 932 2726 3158 3905 4173 932
Size: 1794 432 747 268 1660
Fragments arranged by size:
1794 1660 747 432 268
AfeI AGC'GCT
Cuts at: 1102 1102
Size: 4901
AflIII A'CryG_T
Cuts at: 2253 3357 4334 2253
Size: 1104 977 2820
Fragments arranged by size:
2820 1104 977
AgeI A'CCGG_T
Cuts at: 3289 3289
Size: 4901
AleI CACnn'nnGTG
Cuts at: 590 661 739 590
Size: 71 78 4752
Fragments arranged by size:
4752 78 71
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1418 1418
Size: 4901
AlwNI CAG_nnn'CTG
Cuts at: 1585 2206 3773 1585
Size: 621 1567 2713
Fragments arranged by size:
2713 1567 621
ApaLI G'TGCA_C
Cuts at: 1356 3671 1356
Size: 2315 2586
ApoI r'AATT_y
Cuts at: 1905 4254 4510 1905
Size: 2349 256 2296
Fragments arranged by size:
2349 2296 256
AseI AT'TA_AT
Cuts at: 20 847 20
Size: 827 4074
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4901
AvaI C'yCGr_G
Cuts at: 1211 1921 1211
Size: 710 4191
BamHI G'GATC_C
Cuts at: 1764 1926 1764
Size: 162 4739
BanI G'GyrC_C
Cuts at: 163 769 1917 2331 2579 2614 163
Size: 606 1148 414 248 35 2450
Fragments arranged by size:
2450 1148 606 414 248 35
BanII G_rGCy'C
Cuts at: 238 1915 2945 238
Size: 1677 1030 2194
Fragments arranged by size:
2194 1677 1030
BbeI G_GCGC'C
Cuts at: 2335 2583 2335
Size: 248 4653
BbvCI CC'TCA_GC
Cuts at: 119 119
Size: 4901
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 426 460 426
Size: 34 4867
BciVI GTATCCnnnnn_n'
Cuts at: 2799 3560 4901 2799
Size: 761 1341 2799
Fragments arranged by size:
2799 1341 761
BclI T'GATC_A
Cuts at: 1221 2421 1221
Size: 1200 3701
BfrBI ATG'CAT
Cuts at: 1883 1883
Size: 4901
BglII A'GATC_T
Cuts at: 2416 4453 2416
Size: 2037 2864
BlpI GC'TnA_GC
Cuts at: 1007 2013 1007
Size: 1006 3895
Bme1580I G_kGCm'C
Cuts at: 166 772 1360 1808 2526 2619 3675 166
Size: 606 588 448 718 93 1056 1392
Fragments arranged by size:
1392 1056 718 606 588 448 93
BmgBI CAC'GTC
Cuts at: 681 681
Size: 4901
BmrI ACTGGGnnnn_n'
Cuts at: 415 2113 2194 2529 4673 415
Size: 1698 81 335 2144 643
Fragments arranged by size:
2144 1698 643 335 81
BmtI G_CTAG'C
Cuts at: 1190 1190
Size: 4901
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 1163 3268 1163
Size: 2105 2796
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1342 1653 1342
Size: 311 4590
Bpu10I CC'TnA_GC
Cuts at: 119 1097 1575 119
Size: 978 478 3445
Fragments arranged by size:
3445 978 478
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2077 2956 3448 3746 2077
Size: 879 492 298 3232
Fragments arranged by size:
3232 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 4605 4605
Size: 4901
BsaAI yAC'GTr
Cuts at: 2254 2254
Size: 4901
BsaBI GATnn'nnATC
Cuts at: 6 2415 4452 6
Size: 2409 2037 455
Fragments arranged by size:
2409 2037 455
BsaHI Gr'CG_yC
Cuts at: 2332 2580 3282 4778 2332
Size: 248 702 1496 2455
Fragments arranged by size:
2455 1496 702 248
BsaWI w'CCGG_w
Cuts at: 330 1229 2090 2611 3289 3563 3710 4121 330
Size: 899 861 521 678 274 147 411 1110
Fragments arranged by size:
1110 899 861 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 676 706 676
Size: 30 4871
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 157 157
Size: 4901
BseYI C'CCAG_C
Cuts at: 1011 2326 3084 3661 4157 1011
Size: 1315 758 577 496 1755
Fragments arranged by size:
1755 1315 758 577 496
BsiEI CG_ry'CG
Cuts at: 104 342 675 2443 2489 3697 104
Size: 238 333 1768 46 1208 1308
Fragments arranged by size:
1768 1308 1208 333 238 46
BsiHKAI G_wGCw'C
Cuts at: 238 611 1360 1915 2693 2883 3675 238
Size: 373 749 555 778 190 792 1464
Fragments arranged by size:
1464 792 778 749 555 373 190
BsmBI CGTCTCn'nnnn_
Cuts at: 1389 1671 1761 1389
Size: 282 90 4529
Fragments arranged by size:
4529 282 90
Bsp1286I G_dGCh'C
Cuts at: 166 238 611 772 1360 1808 1915 2526 2619
Size: 72 373 161 588 448 107 611 93
Cuts at: 2619 2693 2883 2945 3675 166
Size: 74 190 62 730 1392
Fragments arranged by size:
1392 730 611 588 448 373 190 161
107 93 74 72 62
BspEI T'CCGG_A
Cuts at: 330 1229 4121 330
Size: 899 2892 1110
Fragments arranged by size:
2892 1110 899
BspHI T'CATG_A
Cuts at: 299 476 4077 299
Size: 177 3601 1123
Fragments arranged by size:
3601 1123 177
BspMI ACCTGCnnnn'nnnn_
Cuts at: 162 1951 2165 2467 2848 162
Size: 1789 214 302 381 2215
Fragments arranged by size:
2215 1789 381 302 214
BsrBI CCG'CTC
Cuts at: 50 3193 3247 4731 4896 50
Size: 3143 54 1484 165 55
Fragments arranged by size:
3143 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 524 595 1175 2813 524
Size: 71 580 1638 2612
Fragments arranged by size:
2612 1638 580 71
BsrFI r'CCGG_y
Cuts at: 2899 3080 3289 2899
Size: 181 209 4511
Fragments arranged by size:
4511 209 181
BssSI C'ACGA_G
Cuts at: 231 3172 3530 231
Size: 2941 358 1602
Fragments arranged by size:
2941 1602 358
BstAPI GCAn_nnn'nTGC
Cuts at: 1417 2278 1417
Size: 861 4040
BstBI TT'CG_AA
Cuts at: 1597 3262 1597
Size: 1665 3236
BstXI CCAn_nnnn'nTGG
Cuts at: 741 741
Size: 4901
BstYI r'GATC_y
Cuts at: 1 318 1764 1926 2393 2416 2751 2997 4009
Size: 317 1446 162 467 23 335 246 1012
Cuts at: 4009 4095 4107 4453 1
Size: 86 12 346 449
Fragments arranged by size:
1446 1012 467 449 346 335 317 246
162 86 23 12
BtgI C'CryG_G
Cuts at: 107 1164 1388 1760 107
Size: 1057 224 372 3248
Fragments arranged by size:
3248 1057 372 224
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 2851 3032 3195 2851
Size: 181 163 4557
Fragments arranged by size:
4557 181 163
BtsI GCAGTG_nn'
Cuts at: 609 734 1799 2144 609
Size: 125 1065 345 3366
Fragments arranged by size:
3366 1065 345 125
DraI TTT'AAA
Cuts at: 1900 1900
Size: 4901
DrdI GACnn_nn'nnGTC
Cuts at: 346 2607 3465 346
Size: 2261 858 1782
Fragments arranged by size:
2261 1782 858
EaeI y'GGCC_r
Cuts at: 188 240 672 746 1871 2486 2660 3051 3078
Size: 52 432 74 1125 615 174 391 27
Cuts at: 3078 4423 188
Size: 1345 666
Fragments arranged by size:
1345 1125 666 615 432 391 174 74
52 27
EagI C'GGCC_G
Cuts at: 672 2486 672
Size: 1814 3087
EarI CTCTTCn'nnn_
Cuts at: 2924 3134 2924
Size: 210 4691
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 127 1504 3109 3419 3565 4711 127
Size: 1377 1605 310 146 1146 317
Fragments arranged by size:
1605 1377 1146 317 310 146
EcoICRI GAG'CTC
Cuts at: 236 1913 236
Size: 1677 3224
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 932 1342 1653 2726 3158 3905 4173 932
Size: 410 311 1073 432 747 268 1660
Fragments arranged by size:
1660 1073 747 432 410 311 268
EcoO109I rG'GnC_Cy
Cuts at: 1509 2040 1509
Size: 531 4370
EcoRI G'AATT_C
Cuts at: 1905 1905
Size: 4901
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2920 2920
Size: 4901
FspI TGC'GCA
Cuts at: 1749 2682 1749
Size: 933 3968
HaeII r_GCGC'y
Cuts at: 636 1104 2335 2583 3605 4584 636
Size: 468 1231 248 1022 979 953
Fragments arranged by size:
1231 1022 979 953 468 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 262 294 335 367 475 507 927 959 963
Size: 32 41 32 108 32 420 32 4
Cuts at: 963 995 1369 1401 2743 2773 2775 2805 262
Size: 32 374 32 1342 30 2 30 2358
Fragments arranged by size:
2358 1342 420 374 108 41 32 32
32 32 32 32 30 30 4 2
HincII GTy'rAC
Cuts at: 918 1940 918
Size: 1022 3879
KasI G'GCGC_C
Cuts at: 2331 2579 2331
Size: 248 4653
KpnI G_GTAC'C
Cuts at: 1921 1921
Size: 4901
MluI A'CGCG_T
Cuts at: 4334 4334
Size: 4901
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 3572 3756 3572
Size: 184 4717
MscI TGG'CCA
Cuts at: 190 748 2662 190
Size: 558 1914 2429
Fragments arranged by size:
2429 1914 558
MslI CAynn'nnrTG
Cuts at: 280 590 661 739 1702 2890 3017 280
Size: 310 71 78 963 1188 127 2164
Fragments arranged by size:
2164 1188 963 310 127 78 71
MspA1I CmG'CkG
Cuts at: 1582 2012 2326 2686 3699 3944 1582
Size: 430 314 360 1013 245 2539
Fragments arranged by size:
2539 1013 430 360 314 245
NaeI GCC'GGC
Cuts at: 3082 3082
Size: 4901
NarI GG'CG_CC
Cuts at: 2332 2580 2332
Size: 248 4653
NcoI C'CATG_G
Cuts at: 107 1760 107
Size: 1653 3248
NgoMIV G'CCGG_C
Cuts at: 3080 3080
Size: 4901
NheI G'CTAG_C
Cuts at: 1186 1186
Size: 4901
NsiI A_TGCA'T
Cuts at: 1885 1885
Size: 4901
NspI r_CATG'y
Cuts at: 227 419 805 992 1399 1887 1954 2454 3361
Size: 192 386 187 407 488 67 500 907
Cuts at: 3361 227
Size: 1767
Fragments arranged by size:
1767 907 500 488 407 386 192 187
67
PciI A'CATG_T
Cuts at: 3357 3357
Size: 4901
PfoI T'CCnGG_A
Cuts at: 137 137
Size: 4901
PmeI GTTT'AAAC
Cuts at: 1900 1900
Size: 4901
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4040 4072 4040
Size: 32 4869
PpuMI rG'GwC_Cy
Cuts at: 1509 1509
Size: 4901
PspXI vC'TCGA_Gb
Cuts at: 1211 1211
Size: 4901
PstI C_TGCA'G
Cuts at: 1948 2633 1948
Size: 685 4216
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4901
PvuII CAG'CTG
Cuts at: 1582 2326 2686 1582
Size: 744 360 3797
Fragments arranged by size:
3797 744 360
RsrII CG'GwC_CG
Cuts at: 3096 3096
Size: 4901
SacI G_AGCT'C
Cuts at: 238 1915 238
Size: 1677 3224
SalI G'TCGA_C
Cuts at: 1938 1938
Size: 4901
SapI GCTCTTCn'nnn_
Cuts at: 2924 3134 2924
Size: 210 4691
SbfI CC_TGCA'GG
Cuts at: 1948 1948
Size: 4901
ScaI AGT'ACT
Cuts at: 442 4418 442
Size: 3976 925
SexAI A'CCwGG_T
Cuts at: 1054 1054
Size: 4901
SfcI C'TryA_G
Cuts at: 33 537 1944 2629 3622 3813 33
Size: 504 1407 685 993 191 1121
Fragments arranged by size:
1407 1121 993 685 504 191
SfoI GGC'GCC
Cuts at: 2333 2581 2333
Size: 248 4653
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4901
SmaI CCC'GGG
Cuts at: 1923 1923
Size: 4901
SmlI C'TyrA_G
Cuts at: 1211 2056 2971 3463 3725 1211
Size: 845 915 492 262 2387
Fragments arranged by size:
2387 915 845 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4901
SphI G_CATG'C
Cuts at: 805 992 1399 1954 2454 805
Size: 187 407 555 500 3252
Fragments arranged by size:
3252 555 500 407 187
StuI AGG'CCT
Cuts at: 1241 1241
Size: 4901
StyI C'CwwG_G
Cuts at: 107 1421 1505 1760 2035 2389 107
Size: 1314 84 255 275 354 2619
Fragments arranged by size:
2619 1314 354 275 255 84
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 356 638 2484 2617 3076 3285 356
Size: 282 1846 133 459 209 1972
Fragments arranged by size:
1972 1846 459 282 209 133
TatI w'GTAC_w
Cuts at: 440 4416 440
Size: 3976 925
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 676 708 1547 1579 4418 4450 676
Size: 32 839 32 2839 32 1127
Fragments arranged by size:
2839 1127 839 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1534 2698 1534
Size: 1164 3737
XbaI T'CTAG_A
Cuts at: 1932 1932
Size: 4901
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 741 1644 2396 741
Size: 903 752 3246
Fragments arranged by size:
3246 903 752
XhoI C'TCGA_G
Cuts at: 1211 1211
Size: 4901
XmaI C'CCGG_G
Cuts at: 1921 1921
Size: 4901
XmnI GAAnn'nnTTC
Cuts at: 207 207
Size: 4901
Enzymes that do cut:
AarI AccI Acc65I AclI AcuI AfeI AflIII AgeI
AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI
BamHI BanI BanII BbeI BbvCI BcgI BciVI BclI
BfrBI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI
BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BsaXI BseRI BseYI BsiEI BsiHKAI BsmBI Bsp1286I BspEI
BspHI BspMI BsrBI BsrDI BsrFI BssSI BstAPI BstBI
BstXI BstYI BtgI BtgZI BtsI DraI DrdI EaeI
EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI FalI
FspI HaeII Hin4I HincII KasI KpnI MluI MmeI
MscI MslI MspA1I NaeI NarI NcoI NgoMIV NheI
NsiI NspI PciI PfoI PmeI PpiI PpuMI PspXI
PstI PvuI PvuII RsrII SacI SalI SapI SbfI
ScaI SexAI SfcI SfoI SgfI SmaI SmlI SpeI
SphI StuI StyI TaqII TatI TstI Tth111I XbaI
XcmI XhoI XmaI XmnI
Enzymes that do not cut:
AatII AflII AhdI AloI ApaI AscI AvrII BaeI
BbsI BglI BsgI BsiWI BsmI BsrGI BssHII BstEII
BstZ17I Bsu36I ClaI CspCI DraIII EcoNI EcoRV FseI
FspAI HindIII HpaI MfeI NdeI NotI NruI PacI
PasI PflMI PmlI PshAI PsiI PspOMI PsrI SacII
SanDI SfiI SgrAI SnaBI SrfI SspI SwaI ZraI