Restriction map for
pF1KE0302
(Circular) (Six-base) MAPSORT of: pF1KE0302.seq Check: 1059 from: 1 to: 4541
pF1KE0302 4541 bp
With 182 enzymes: SgfI *
April 24, 2009 13:12 ..
AccI GT'mk_AC
Cuts at: 1579 1579
Size: 4541
Acc65I G'GTAC_C
Cuts at: 1037 1557 1037
Size: 520 4021
AclI AA'CG_TT
Cuts at: 4381 4381
Size: 4541
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 2366 2798 3545 3813 2366
Size: 432 747 268 3094
Fragments arranged by size:
3094 747 432 268
AfeI AGC'GCT
Cuts at: 226 226
Size: 4541
AflIII A'CryG_T
Cuts at: 528 1893 2997 3974 528
Size: 1365 1104 977 1095
Fragments arranged by size:
1365 1104 1095 977
AgeI A'CCGG_T
Cuts at: 2929 2929
Size: 4541
AleI CACnn'nnGTG
Cuts at: 160 160
Size: 4541
AlwNI CAG_nnn'CTG
Cuts at: 491 1846 3413 491
Size: 1355 1567 1619
Fragments arranged by size:
1619 1567 1355
ApaLI G'TGCA_C
Cuts at: 382 3311 382
Size: 2929 1612
ApoI r'AATT_y
Cuts at: 1545 3894 4150 1545
Size: 2349 256 1936
Fragments arranged by size:
2349 1936 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 4541
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4541
AvaI C'yCGr_G
Cuts at: 210 1199 1207 1561 210
Size: 989 8 354 3190
Fragments arranged by size:
3190 989 354 8
BamHI G'GATC_C
Cuts at: 833 957 1566 833
Size: 124 609 3808
Fragments arranged by size:
3808 609 124
BanI G'GyrC_C
Cuts at: 1037 1557 1971 2219 2254 1037
Size: 520 414 248 35 3324
Fragments arranged by size:
3324 520 414 248 35
BanII G_rGCy'C
Cuts at: 169 181 217 756 1321 1399 1555 2585 169
Size: 12 36 539 565 78 156 1030 2125
Fragments arranged by size:
2125 1030 565 539 156 78 36 12
BbeI G_GCGC'C
Cuts at: 1975 2223 1975
Size: 248 4293
BbsI GAAGACnn'nnnn_
Cuts at: 140 531 880 140
Size: 391 349 3801
Fragments arranged by size:
3801 391 349
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 106 140 981 1015 106
Size: 34 841 34 3632
Fragments arranged by size:
3632 841 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 406 2439 3200 4541 406
Size: 2033 761 1341 406
Fragments arranged by size:
2033 1341 761 406
BclI T'GATC_A
Cuts at: 362 1424 2061 362
Size: 1062 637 2842
Fragments arranged by size:
2842 1062 637
BfrBI ATG'CAT
Cuts at: 1268 1268
Size: 4541
BglII A'GATC_T
Cuts at: 2056 4093 2056
Size: 2037 2504
BlpI GC'TnA_GC
Cuts at: 1653 1653
Size: 4541
Bme1580I G_kGCm'C
Cuts at: 386 2166 2259 3315 386
Size: 1780 93 1056 1612
Fragments arranged by size:
1780 1612 1056 93
BmgBI CAC'GTC
Cuts at: 162 529 162
Size: 367 4174
BmrI ACTGGGnnnn_n'
Cuts at: 528 1753 1834 2169 4313 528
Size: 1225 81 335 2144 756
Fragments arranged by size:
2144 1225 756 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2908 2908
Size: 4541
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1138 1384 1138
Size: 246 4295
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1717 2596 3088 3386 1717
Size: 879 492 298 2872
Fragments arranged by size:
2872 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 4245 4245
Size: 4541
BsaAI yAC'GTr
Cuts at: 1894 1894
Size: 4541
BsaBI GATnn'nnATC
Cuts at: 6 1180 1429 2055 4092 6
Size: 1174 249 626 2037 455
Fragments arranged by size:
2037 1174 626 455 249
BsaHI Gr'CG_yC
Cuts at: 673 1418 1972 2220 2922 4418 673
Size: 745 554 248 702 1496 796
Fragments arranged by size:
1496 796 745 702 554 248
BsaWI w'CCGG_w
Cuts at: 503 1730 2251 2929 3203 3350 3761 503
Size: 1227 521 678 274 147 411 1283
Fragments arranged by size:
1283 1227 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 136 166 187 190 196 217 220 688 718
Size: 30 21 3 6 21 3 468 30
Cuts at: 718 136
Size: 3959
Fragments arranged by size:
3959 468 30 30 21 21 6 3
3
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 187 195 187
Size: 8 4533
BseYI C'CCAG_C
Cuts at: 557 561 844 1966 2724 3301 3797 557
Size: 4 283 1122 758 577 496 1301
Fragments arranged by size:
1301 1122 758 577 496 283 4
BsiEI CG_ry'CG
Cuts at: 104 2083 2129 3337 104
Size: 1979 46 1208 1308
Fragments arranged by size:
1979 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 386 414 1214 1555 2333 2523 3315 386
Size: 28 800 341 778 190 792 1612
Fragments arranged by size:
1612 800 792 778 341 190 28
BsmBI CGTCTCn'nnnn_
Cuts at: 652 652
Size: 4541
Bsp1286I G_dGCh'C
Cuts at: 169 181 217 386 414 756 1214 1321 1399
Size: 12 36 169 28 342 458 107 78
Cuts at: 1399 1555 2166 2259 2333 2523 2585 3315 169
Size: 156 611 93 74 190 62 730 1395
Fragments arranged by size:
1395 730 611 458 342 190 169 156
107 93 78 74 62 36 28 12
BspEI T'CCGG_A
Cuts at: 503 3761 503
Size: 3258 1283
BspHI T'CATG_A
Cuts at: 806 3717 806
Size: 2911 1630
BspMI ACCTGCnnnn'nnnn_
Cuts at: 1049 1091 1591 1805 2107 2488 1049
Size: 42 500 214 302 381 3102
Fragments arranged by size:
3102 500 381 302 214 42
BsrBI CCG'CTC
Cuts at: 50 1359 2833 2887 4371 4536 50
Size: 1309 1474 54 1484 165 55
Fragments arranged by size:
1484 1474 1309 165 55 54
BsrDI GCAATG_nn'
Cuts at: 1503 2453 1503
Size: 950 3591
BsrFI r'CCGG_y
Cuts at: 2539 2720 2929 2539
Size: 181 209 4151
Fragments arranged by size:
4151 209 181
BssSI C'ACGA_G
Cuts at: 2812 3170 2812
Size: 358 4183
BstAPI GCAn_nnn'nTGC
Cuts at: 1918 1918
Size: 4541
BstBI TT'CG_AA
Cuts at: 2902 2902
Size: 4541
BstEII G'GTnAC_C
Cuts at: 1368 1368
Size: 4541
BstXI CCAn_nnnn'nTGG
Cuts at: 229 229
Size: 4541
BstYI r'GATC_y
Cuts at: 1 833 957 1133 1566 2033 2056 2391 2637
Size: 832 124 176 433 467 23 335 246
Cuts at: 2637 3649 3735 3747 4093 1
Size: 1012 86 12 346 449
Fragments arranged by size:
1012 832 467 449 433 346 335 246
176 124 86 23 12
Bsu36I CC'TnA_GG
Cuts at: 170 170
Size: 4541
BtgI C'CryG_G
Cuts at: 155 680 155
Size: 525 4016
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 2491 2672 2835 2491
Size: 181 163 4197
Fragments arranged by size:
4197 181 163
BtsI GCAGTG_nn'
Cuts at: 384 1784 384
Size: 1400 3141
DraI TTT'AAA
Cuts at: 606 1540 606
Size: 934 3607
DrdI GACnn_nn'nnGTC
Cuts at: 2247 3105 2247
Size: 858 3683
EaeI y'GGCC_r
Cuts at: 377 623 776 794 1274 2126 2300 2691 2718
Size: 246 153 18 480 852 174 391 27
Cuts at: 2718 4063 377
Size: 1345 855
Fragments arranged by size:
1345 855 852 480 391 246 174 153
27 18
EagI C'GGCC_G
Cuts at: 2126 2126
Size: 4541
EarI CTCTTCn'nnn_
Cuts at: 892 2564 2774 892
Size: 1672 210 2659
Fragments arranged by size:
2659 1672 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2749 3059 3205 4351 2749
Size: 310 146 1146 2939
Fragments arranged by size:
2939 1146 310 146
EcoICRI GAG'CTC
Cuts at: 1553 1553
Size: 4541
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1138 1384 2366 2798 3545 3813 1138
Size: 246 982 432 747 268 1866
Fragments arranged by size:
1866 982 747 432 268 246
EcoO109I rG'GnC_Cy
Cuts at: 346 1460 1680 346
Size: 1114 220 3207
Fragments arranged by size:
3207 1114 220
EcoRI G'AATT_C
Cuts at: 1545 1545
Size: 4541
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2560 2560
Size: 4541
FspI TGC'GCA
Cuts at: 1243 2322 1243
Size: 1079 3462
FspAI rTGC'GCAy
Cuts at: 1243 1243
Size: 4541
HaeII r_GCGC'y
Cuts at: 228 1207 1337 1975 2223 3245 4224 228
Size: 979 130 638 248 1022 979 545
Fragments arranged by size:
1022 979 979 638 545 248 130
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 136 166 168 198 867 899 1043 1075 2383
Size: 30 2 30 669 32 144 32 1308
Cuts at: 2383 2413 2415 2445 136
Size: 30 2 30 2232
Fragments arranged by size:
2232 1308 669 144 32 32 30 30
30 30 2 2
HincII GTy'rAC
Cuts at: 1580 1580
Size: 4541
KasI G'GCGC_C
Cuts at: 1971 2219 1971
Size: 248 4293
KpnI G_GTAC'C
Cuts at: 1041 1561 1041
Size: 520 4021
MluI A'CGCG_T
Cuts at: 3974 3974
Size: 4541
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 164 3212 3396 164
Size: 3048 184 1309
Fragments arranged by size:
3048 1309 184
MscI TGG'CCA
Cuts at: 778 796 1276 2302 778
Size: 18 480 1026 3017
Fragments arranged by size:
3017 1026 480 18
MslI CAynn'nnrTG
Cuts at: 160 340 653 787 925 2530 2657 160
Size: 180 313 134 138 1605 127 2044
Fragments arranged by size:
2044 1605 313 180 138 134 127
MspA1I CmG'CkG
Cuts at: 561 1652 1966 2326 3339 3584 561
Size: 1091 314 360 1013 245 1518
Fragments arranged by size:
1518 1091 1013 360 314 245
NaeI GCC'GGC
Cuts at: 2722 2722
Size: 4541
NarI GG'CG_CC
Cuts at: 1972 2220 1972
Size: 248 4293
NcoI C'CATG_G
Cuts at: 680 680
Size: 4541
NgoMIV G'CCGG_C
Cuts at: 2720 2720
Size: 4541
NruI TCG'CGA
Cuts at: 690 690
Size: 4541
NsiI A_TGCA'T
Cuts at: 1270 1270
Size: 4541
NspI r_CATG'y
Cuts at: 702 1268 1594 2094 3001 702
Size: 566 326 500 907 2242
Fragments arranged by size:
2242 907 566 500 326
PciI A'CATG_T
Cuts at: 2997 2997
Size: 4541
PflMI CCAn_nnn'nTGG
Cuts at: 1094 1094
Size: 4541
PfoI T'CCnGG_A
Cuts at: 1361 1361
Size: 4541
PmeI GTTT'AAAC
Cuts at: 1540 1540
Size: 4541
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3680 3712 3680
Size: 32 4509
PpuMI rG'GwC_Cy
Cuts at: 346 1460 346
Size: 1114 3427
PstI C_TGCA'G
Cuts at: 1588 2273 1588
Size: 685 3856
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4541
PvuII CAG'CTG
Cuts at: 561 1966 2326 561
Size: 1405 360 2776
Fragments arranged by size:
2776 1405 360
RsrII CG'GwC_CG
Cuts at: 2736 2736
Size: 4541
SacI G_AGCT'C
Cuts at: 1555 1555
Size: 4541
SalI G'TCGA_C
Cuts at: 1578 1578
Size: 4541
SanDI GG'GwC_CC
Cuts at: 346 346
Size: 4541
SapI GCTCTTCn'nnn_
Cuts at: 2564 2774 2564
Size: 210 4331
SbfI CC_TGCA'GG
Cuts at: 1588 1588
Size: 4541
ScaI AGT'ACT
Cuts at: 4058 4058
Size: 4541
SexAI A'CCwGG_T
Cuts at: 1298 1298
Size: 4541
SfcI C'TryA_G
Cuts at: 33 147 1584 2269 3262 3453 33
Size: 114 1437 685 993 191 1121
Fragments arranged by size:
1437 1121 993 685 191 114
SfoI GGC'GCC
Cuts at: 1973 2221 1973
Size: 248 4293
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4541
SmaI CCC'GGG
Cuts at: 212 1563 212
Size: 1351 3190
SmlI C'TyrA_G
Cuts at: 1696 2611 3103 3365 1696
Size: 915 492 262 2872
Fragments arranged by size:
2872 915 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4541
SphI G_CATG'C
Cuts at: 1594 2094 1594
Size: 500 4041
StuI AGG'CCT
Cuts at: 191 191
Size: 4541
StyI C'CwwG_G
Cuts at: 680 857 868 1062 1675 2029 680
Size: 177 11 194 613 354 3192
Fragments arranged by size:
3192 613 354 194 177 11
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 571 2124 2257 2716 2925 571
Size: 1553 133 459 209 2187
Fragments arranged by size:
2187 1553 459 209 133
TatI w'GTAC_w
Cuts at: 1121 4056 1121
Size: 2935 1606
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 4058 4090 4058
Size: 32 4509
Tth111I GACn'n_nGTC
Cuts at: 1300 2338 1300
Size: 1038 3503
XbaI T'CTAG_A
Cuts at: 1572 1572
Size: 4541
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2036 2036
Size: 4541
XmaI C'CCGG_G
Cuts at: 210 1561 210
Size: 1351 3190
Enzymes that do cut:
AccI Acc65I AclI AcuI AfeI AflIII AgeI AleI
AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI
BanII BbeI BbsI BcgI BciVI BclI BfrBI BglII
BlpI Bme1580I BmgBI BmrI BplI BpmI BpuEI BsaI
BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsiEI
BsiHKAI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI
BsrFI BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I
BtgI BtgZI BtsI DraI DrdI EaeI EagI EarI
EciI EcoICRI Eco57MI EcoO109I EcoRI FalI FspI FspAI
HaeII Hin4I HincII KasI KpnI MluI MmeI MscI
MslI MspA1I NaeI NarI NcoI NgoMIV NruI NsiI
NspI PciI PflMI PfoI PmeI PpiI PpuMI PstI
PvuI PvuII RsrII SacI SalI SanDI SapI SbfI
ScaI SexAI SfcI SfoI SgfI SmaI SmlI SpeI
SphI StuI StyI TaqII TatI TstI Tth111I XbaI
XcmI XmaI
Enzymes that do not cut:
AarI AatII AflII AhdI AlfI AloI ApaI AscI
AvrII BaeI BbvCI BglI BmtI Bpu10I BsgI BsiWI
BsmI BsrGI BssHII BstZ17I ClaI CspCI DraIII EcoNI
EcoRV FseI HindIII HpaI MfeI NdeI NheI NotI
PacI PasI PmlI PshAI PsiI PspOMI PspXI PsrI
SacII SfiI SgrAI SnaBI SrfI SspI SwaI XhoI
XmnI ZraI