Restriction map for
pF1KE0295
(Circular) (Six-base) MAPSORT of: pF1KE0295.seq Check: 1716 from: 1 to: 3821
pF1KE0295 3821 bp
With 182 enzymes: SgfI *
April 24, 2009 13:12 ..
AccI GT'mk_AC
Cuts at: 859 859
Size: 3821
Acc65I G'GTAC_C
Cuts at: 837 837
Size: 3821
AclI AA'CG_TT
Cuts at: 3661 3661
Size: 3821
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1646 2078 2825 3093 1646
Size: 432 747 268 2374
Fragments arranged by size:
2374 747 432 268
AflIII A'CryG_T
Cuts at: 1173 2277 3254 1173
Size: 1104 977 1740
Fragments arranged by size:
1740 1104 977
AgeI A'CCGG_T
Cuts at: 2209 2209
Size: 3821
AlwNI CAG_nnn'CTG
Cuts at: 1126 2693 1126
Size: 1567 2254
ApaI G_GGCC'C
Cuts at: 440 600 768 440
Size: 160 168 3493
Fragments arranged by size:
3493 168 160
ApaLI G'TGCA_C
Cuts at: 688 2591 688
Size: 1903 1918
ApoI r'AATT_y
Cuts at: 825 3174 3430 825
Size: 2349 256 1216
Fragments arranged by size:
2349 1216 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 3821
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 3821
AvaI C'yCGr_G
Cuts at: 122 181 269 524 593 599 841 122
Size: 59 88 255 69 6 242 3102
Fragments arranged by size:
3102 255 242 88 69 59 6
BamHI G'GATC_C
Cuts at: 846 846
Size: 3821
BanI G'GyrC_C
Cuts at: 117 253 391 503 511 589 837 1251 1499
Size: 136 138 112 8 78 248 414 248
Cuts at: 1499 1534 117
Size: 35 2404
Fragments arranged by size:
2404 414 248 248 138 136 112 78
35 8
BanII G_rGCy'C
Cuts at: 167 182 440 600 768 779 835 1865 167
Size: 15 258 160 168 11 56 1030 2123
Fragments arranged by size:
2123 1030 258 168 160 56 15 11
BbeI G_GCGC'C
Cuts at: 395 507 1255 1503 395
Size: 112 748 248 2713
Fragments arranged by size:
2713 748 248 112
BbsI GAAGACnn'nnnn_
Cuts at: 132 132
Size: 3821
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 235 269 235
Size: 34 3787
BciVI GTATCCnnnnn_n'
Cuts at: 1719 2480 3821 1719
Size: 761 1341 1719
Fragments arranged by size:
1719 1341 761
BclI T'GATC_A
Cuts at: 1341 1341
Size: 3821
BglII A'GATC_T
Cuts at: 1336 3373 1336
Size: 2037 1784
BlpI GC'TnA_GC
Cuts at: 933 933
Size: 3821
Bme1580I G_kGCm'C
Cuts at: 258 304 440 516 600 692 768 1446 1539
Size: 46 136 76 84 92 76 678 93
Cuts at: 1539 2595 258
Size: 1056 1484
Fragments arranged by size:
1484 1056 678 136 93 92 84 76
76 46
BmgBI CAC'GTC
Cuts at: 687 687
Size: 3821
BmrI ACTGGGnnnn_n'
Cuts at: 164 170 1033 1114 1449 3593 164
Size: 6 863 81 335 2144 392
Fragments arranged by size:
2144 863 392 335 81 6
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2188 2188
Size: 3821
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 247 829 247
Size: 582 3239
Bpu10I CC'TnA_GC
Cuts at: 346 421 346
Size: 75 3746
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 997 1876 2368 2666 997
Size: 879 492 298 2152
Fragments arranged by size:
2152 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 124 3525 124
Size: 3401 420
BsaAI yAC'GTr
Cuts at: 1174 1174
Size: 3821
BsaBI GATnn'nnATC
Cuts at: 6 1335 3372 6
Size: 1329 2037 455
Fragments arranged by size:
2037 1329 455
BsaHI Gr'CG_yC
Cuts at: 392 504 1252 1500 2202 3698 392
Size: 112 748 248 702 1496 515
Fragments arranged by size:
1496 748 702 515 248 112
BsaWI w'CCGG_w
Cuts at: 1010 1531 2209 2483 2630 3041 1010
Size: 521 678 274 147 411 1790
Fragments arranged by size:
1790 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 205 235 205
Size: 30 3791
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 210 210
Size: 3821
BseYI C'CCAG_C
Cuts at: 802 1246 2004 2581 3077 802
Size: 444 758 577 496 1546
Fragments arranged by size:
1546 758 577 496 444
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 697 697
Size: 3821
BsiEI CG_ry'CG
Cuts at: 104 1363 1409 2617 104
Size: 1259 46 1208 1308
Fragments arranged by size:
1308 1259 1208 46
BsiHKAI G_wGCw'C
Cuts at: 242 692 835 1613 1803 2595 242
Size: 450 143 778 190 792 1468
Fragments arranged by size:
1468 792 778 450 190 143
Bsp1286I G_dGCh'C
Cuts at: 167 182 242 258 304 440 516 600 692
Size: 15 60 16 46 136 76 84 92
Cuts at: 692 768 779 835 1446 1539 1613 1803 1865
Size: 76 11 56 611 93 74 190 62
Cuts at: 1865 2595 167
Size: 730 1393
Fragments arranged by size:
1393 730 611 190 136 93 92 84
76 76 74 62 60 56 46 16
15 11
BspEI T'CCGG_A
Cuts at: 3041 3041
Size: 3821
BspHI T'CATG_A
Cuts at: 2997 2997
Size: 3821
BspMI ACCTGCnnnn'nnnn_
Cuts at: 871 1085 1387 1768 871
Size: 214 302 381 2924
Fragments arranged by size:
2924 381 302 214
BsrBI CCG'CTC
Cuts at: 50 218 2113 2167 3651 3816 50
Size: 168 1895 54 1484 165 55
Fragments arranged by size:
1895 1484 168 165 55 54
BsrDI GCAATG_nn'
Cuts at: 1733 1733
Size: 3821
BsrFI r'CCGG_y
Cuts at: 340 552 1819 2000 2209 340
Size: 212 1267 181 209 1952
Fragments arranged by size:
1952 1267 212 209 181
BssSI C'ACGA_G
Cuts at: 2092 2450 2092
Size: 358 3463
BstAPI GCAn_nnn'nTGC
Cuts at: 696 1198 696
Size: 502 3319
BstBI TT'CG_AA
Cuts at: 2182 2182
Size: 3821
BstYI r'GATC_y
Cuts at: 1 846 1313 1336 1671 1917 2929 3015 3027
Size: 845 467 23 335 246 1012 86 12
Cuts at: 3027 3373 1
Size: 346 449
Fragments arranged by size:
1012 845 467 449 346 335 246 86
23 12
BtgI C'CryG_G
Cuts at: 395 585 756 395
Size: 190 171 3460
Fragments arranged by size:
3460 190 171
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 381 1771 1952 2115 381
Size: 1390 181 163 2087
Fragments arranged by size:
2087 1390 181 163
BtsI GCAGTG_nn'
Cuts at: 317 357 1064 317
Size: 40 707 3074
Fragments arranged by size:
3074 707 40
DraI TTT'AAA
Cuts at: 820 820
Size: 3821
DraIII CAC_nnn'GTG
Cuts at: 293 293
Size: 3821
DrdI GACnn_nn'nnGTC
Cuts at: 1527 2385 1527
Size: 858 2963
EaeI y'GGCC_r
Cuts at: 1406 1580 1971 1998 3343 1406
Size: 174 391 27 1345 1884
Fragments arranged by size:
1884 1345 391 174 27
EagI C'GGCC_G
Cuts at: 1406 1406
Size: 3821
EarI CTCTTCn'nnn_
Cuts at: 637 1844 2054 637
Size: 1207 210 2404
Fragments arranged by size:
2404 1207 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2029 2339 2485 3631 2029
Size: 310 146 1146 2219
Fragments arranged by size:
2219 1146 310 146
EcoICRI GAG'CTC
Cuts at: 833 833
Size: 3821
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 247 829 1646 2078 2825 3093 247
Size: 582 817 432 747 268 975
Fragments arranged by size:
975 817 747 582 432 268
EcoO109I rG'GnC_Cy
Cuts at: 273 357 436 564 960 273
Size: 84 79 128 396 3134
Fragments arranged by size:
3134 396 128 84 79
EcoRI G'AATT_C
Cuts at: 825 825
Size: 3821
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 399 1840 399
Size: 1441 2380
FspI TGC'GCA
Cuts at: 1602 1602
Size: 3821
HaeII r_GCGC'y
Cuts at: 312 395 507 1255 1503 2525 3504 312
Size: 83 112 748 248 1022 979 629
Fragments arranged by size:
1022 979 748 629 248 112 83
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1663 1693 1695 1725 1663
Size: 30 2 30 3759
Fragments arranged by size:
3759 30 30 2
HincII GTy'rAC
Cuts at: 860 860
Size: 3821
KasI G'GCGC_C
Cuts at: 391 503 1251 1499 391
Size: 112 748 248 2713
Fragments arranged by size:
2713 748 248 112
KpnI G_GTAC'C
Cuts at: 841 841
Size: 3821
MluI A'CGCG_T
Cuts at: 3254 3254
Size: 3821
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 2492 2676 2492
Size: 184 3637
MscI TGG'CCA
Cuts at: 1582 1582
Size: 3821
MslI CAynn'nnrTG
Cuts at: 1810 1937 1810
Size: 127 3694
MspA1I CmG'CkG
Cuts at: 932 1246 1606 2619 2864 932
Size: 314 360 1013 245 1889
Fragments arranged by size:
1889 1013 360 314 245
NaeI GCC'GGC
Cuts at: 554 2002 554
Size: 1448 2373
NarI GG'CG_CC
Cuts at: 392 504 1252 1500 392
Size: 112 748 248 2713
Fragments arranged by size:
2713 748 248 112
NgoMIV G'CCGG_C
Cuts at: 552 2000 552
Size: 1448 2373
NspI r_CATG'y
Cuts at: 300 874 1374 2281 300
Size: 574 500 907 1840
Fragments arranged by size:
1840 907 574 500
PciI A'CATG_T
Cuts at: 2277 2277
Size: 3821
PfoI T'CCnGG_A
Cuts at: 143 275 143
Size: 132 3689
PmeI GTTT'AAAC
Cuts at: 820 820
Size: 3821
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2960 2992 2960
Size: 32 3789
PpuMI rG'GwC_Cy
Cuts at: 273 273
Size: 3821
PshAI GACnn'nnGTC
Cuts at: 136 136
Size: 3821
PspOMI G'GGCC_C
Cuts at: 436 596 764 436
Size: 160 168 3493
Fragments arranged by size:
3493 168 160
PstI C_TGCA'G
Cuts at: 413 868 1553 413
Size: 455 685 2681
Fragments arranged by size:
2681 685 455
PvuI CG_AT'CG
Cuts at: 104 104
Size: 3821
PvuII CAG'CTG
Cuts at: 1246 1606 1246
Size: 360 3461
RsrII CG'GwC_CG
Cuts at: 2016 2016
Size: 3821
SacI G_AGCT'C
Cuts at: 835 835
Size: 3821
SalI G'TCGA_C
Cuts at: 858 858
Size: 3821
SapI GCTCTTCn'nnn_
Cuts at: 1844 2054 1844
Size: 210 3611
SbfI CC_TGCA'GG
Cuts at: 868 868
Size: 3821
ScaI AGT'ACT
Cuts at: 3338 3338
Size: 3821
SfcI C'TryA_G
Cuts at: 33 409 864 1549 2542 2733 33
Size: 376 455 685 993 191 1121
Fragments arranged by size:
1121 993 685 455 376 191
SfoI GGC'GCC
Cuts at: 393 505 1253 1501 393
Size: 112 748 248 2713
Fragments arranged by size:
2713 748 248 112
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 3821
SmaI CCC'GGG
Cuts at: 124 271 526 595 601 843 124
Size: 147 255 69 6 242 3102
Fragments arranged by size:
3102 255 242 147 69 6
SmlI C'TyrA_G
Cuts at: 976 1891 2383 2645 976
Size: 915 492 262 2152
Fragments arranged by size:
2152 915 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 3821
SphI G_CATG'C
Cuts at: 874 1374 874
Size: 500 3321
StuI AGG'CCT
Cuts at: 415 415
Size: 3821
StyI C'CwwG_G
Cuts at: 323 955 1309 323
Size: 632 354 2835
Fragments arranged by size:
2835 632 354
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 499 749 1404 1537 1996 2205 499
Size: 250 655 133 459 209 2115
Fragments arranged by size:
2115 655 459 250 209 133
TatI w'GTAC_w
Cuts at: 782 3336 782
Size: 2554 1267
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 205 237 3338 3370 205
Size: 32 3101 32 656
Fragments arranged by size:
3101 656 32 32
Tth111I GACn'n_nGTC
Cuts at: 532 1618 532
Size: 1086 2735
XbaI T'CTAG_A
Cuts at: 852 852
Size: 3821
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 447 1316 447
Size: 869 2952
XmaI C'CCGG_G
Cuts at: 122 269 524 593 599 841 122
Size: 147 255 69 6 242 3102
Fragments arranged by size:
3102 255 242 147 69 6
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AgeI AlwNI ApaI
ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII
BbeI BbsI BcgI BciVI BclI BglII BlpI Bme1580I
BmgBI BmrI BplI BpmI Bpu10I BpuEI BsaI BsaAI
BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI
BsiHKAI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BssSI BstAPI BstBI BstYI BtgI BtgZI BtsI DraI
DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI
EcoO109I EcoRI FalI FspI HaeII Hin4I HincII KasI
KpnI MluI MmeI MscI MslI MspA1I NaeI NarI
NgoMIV NspI PciI PfoI PmeI PpiI PpuMI PshAI
PspOMI PstI PvuI PvuII RsrII SacI SalI SapI
SbfI ScaI SfcI SfoI SgfI SmaI SmlI SpeI
SphI StuI StyI TaqII TatI TstI Tth111I XbaI
XcmI XmaI
Enzymes that do not cut:
AarI AatII AfeI AflII AhdI AleI AlfI AloI
AscI AvrII BaeI BbvCI BfrBI BglI BmtI BsiWI
BsmI BsmBI BsrGI BssHII BstEII BstXI BstZ17I Bsu36I
ClaI CspCI EcoNI EcoRV FseI FspAI HindIII HpaI
MfeI NcoI NdeI NheI NotI NruI NsiI PacI
PasI PflMI PmlI PsiI PspXI PsrI SacII SanDI
SexAI SfiI SgrAI SnaBI SrfI SspI SwaI XhoI
XmnI ZraI