Restriction map for
pF1KE0289
(Circular) (Six-base) MAPSORT of: pF1KE0289.seq Check: 7080 from: 1 to: 4049
pF1KE0289 4049 bp
With 182 enzymes: SgfI *
April 24, 2009 13:12 ..
AccI GT'mk_AC
Cuts at: 1087 1087
Size: 4049
Acc65I G'GTAC_C
Cuts at: 865 1065 865
Size: 200 3849
AclI AA'CG_TT
Cuts at: 3889 3889
Size: 4049
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1874 2306 3053 3321 1874
Size: 432 747 268 2602
Fragments arranged by size:
2602 747 432 268
AflIII A'CryG_T
Cuts at: 1401 2505 3482 1401
Size: 1104 977 1968
Fragments arranged by size:
1968 1104 977
AgeI A'CCGG_T
Cuts at: 2437 2437
Size: 4049
AlwNI CAG_nnn'CTG
Cuts at: 1354 2921 1354
Size: 1567 2482
ApaLI G'TGCA_C
Cuts at: 2819 2819
Size: 4049
ApoI r'AATT_y
Cuts at: 1053 3402 3658 1053
Size: 2349 256 1444
Fragments arranged by size:
2349 1444 256
AseI AT'TA_AT
Cuts at: 20 577 20
Size: 557 3492
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4049
AvaI C'yCGr_G
Cuts at: 1069 1069
Size: 4049
BamHI G'GATC_C
Cuts at: 788 1074 788
Size: 286 3763
BanI G'GyrC_C
Cuts at: 865 1065 1479 1727 1762 865
Size: 200 414 248 35 3152
Fragments arranged by size:
3152 414 248 200 35
BanII G_rGCy'C
Cuts at: 1063 2093 1063
Size: 1030 3019
BbeI G_GCGC'C
Cuts at: 1483 1731 1483
Size: 248 3801
BciVI GTATCCnnnnn_n'
Cuts at: 1947 2708 4049 1947
Size: 761 1341 1947
Fragments arranged by size:
1947 1341 761
BclI T'GATC_A
Cuts at: 749 1569 749
Size: 820 3229
BglII A'GATC_T
Cuts at: 1564 3601 1564
Size: 2037 2012
BlpI GC'TnA_GC
Cuts at: 1161 1161
Size: 4049
Bme1580I G_kGCm'C
Cuts at: 1674 1767 2823 1674
Size: 93 1056 2900
Fragments arranged by size:
2900 1056 93
BmrI ACTGGGnnnn_n'
Cuts at: 894 1261 1342 1677 3821 894
Size: 367 81 335 2144 1122
Fragments arranged by size:
2144 1122 367 335 81
BmtI G_CTAG'C
Cuts at: 782 782
Size: 4049
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2416 2416
Size: 4049
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 615 1225 2104 2596 2894 615
Size: 610 879 492 298 1770
Fragments arranged by size:
1770 879 610 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 3753 3753
Size: 4049
BsaAI yAC'GTr
Cuts at: 1402 1402
Size: 4049
BsaBI GATnn'nnATC
Cuts at: 6 1563 3600 6
Size: 1557 2037 455
Fragments arranged by size:
2037 1557 455
BsaHI Gr'CG_yC
Cuts at: 1480 1728 2430 3926 1480
Size: 248 702 1496 1603
Fragments arranged by size:
1603 1496 702 248
BsaWI w'CCGG_w
Cuts at: 715 1238 1759 2437 2711 2858 3269 715
Size: 523 521 678 274 147 411 1495
Fragments arranged by size:
1495 678 523 521 411 274 147
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 612 612
Size: 4049
BseYI C'CCAG_C
Cuts at: 1474 2232 2809 3305 1474
Size: 758 577 496 2218
Fragments arranged by size:
2218 758 577 496
BsiEI CG_ry'CG
Cuts at: 104 1591 1637 2845 104
Size: 1487 46 1208 1308
Fragments arranged by size:
1487 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 1063 1841 2031 2823 1063
Size: 778 190 792 2289
Fragments arranged by size:
2289 792 778 190
Bsp1286I G_dGCh'C
Cuts at: 1063 1674 1767 1841 2031 2093 2823 1063
Size: 611 93 74 190 62 730 2289
Fragments arranged by size:
2289 730 611 190 93 74 62
BspEI T'CCGG_A
Cuts at: 715 3269 715
Size: 2554 1495
BspHI T'CATG_A
Cuts at: 3225 3225
Size: 4049
BspMI ACCTGCnnnn'nnnn_
Cuts at: 1099 1313 1615 1996 1099
Size: 214 302 381 3152
Fragments arranged by size:
3152 381 302 214
BsrBI CCG'CTC
Cuts at: 50 2341 2395 3879 4044 50
Size: 2291 54 1484 165 55
Fragments arranged by size:
2291 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 722 767 1961 722
Size: 45 1194 2810
Fragments arranged by size:
2810 1194 45
BsrFI r'CCGG_y
Cuts at: 2047 2228 2437 2047
Size: 181 209 3659
Fragments arranged by size:
3659 209 181
BssSI C'ACGA_G
Cuts at: 2320 2678 2320
Size: 358 3691
BstAPI GCAn_nnn'nTGC
Cuts at: 1426 1426
Size: 4049
BstBI TT'CG_AA
Cuts at: 2410 2410
Size: 4049
BstXI CCAn_nnnn'nTGG
Cuts at: 351 351
Size: 4049
BstYI r'GATC_y
Cuts at: 1 788 1074 1541 1564 1899 2145 3157 3243
Size: 787 286 467 23 335 246 1012 86
Cuts at: 3243 3255 3601 1
Size: 12 346 449
Fragments arranged by size:
1012 787 467 449 346 335 286 246
86 23 12
Bsu36I CC'TnA_GG
Cuts at: 784 784
Size: 4049
BtgI C'CryG_G
Cuts at: 107 728 107
Size: 621 3428
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 1999 2180 2343 1999
Size: 181 163 3705
Fragments arranged by size:
3705 181 163
BtsI GCAGTG_nn'
Cuts at: 655 1013 1292 655
Size: 358 279 3412
Fragments arranged by size:
3412 358 279
DraI TTT'AAA
Cuts at: 1048 1048
Size: 4049
DrdI GACnn_nn'nnGTC
Cuts at: 1755 2613 1755
Size: 858 3191
EaeI y'GGCC_r
Cuts at: 479 731 1634 1808 2199 2226 3571 479
Size: 252 903 174 391 27 1345 957
Fragments arranged by size:
1345 957 903 391 252 174 27
EagI C'GGCC_G
Cuts at: 1634 1634
Size: 4049
EarI CTCTTCn'nnn_
Cuts at: 2072 2282 2072
Size: 210 3839
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2257 2567 2713 3859 2257
Size: 310 146 1146 2447
Fragments arranged by size:
2447 1146 310 146
EcoICRI GAG'CTC
Cuts at: 1061 1061
Size: 4049
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1874 2306 3053 3321 1874
Size: 432 747 268 2602
Fragments arranged by size:
2602 747 432 268
EcoNI CCTnn'n_nnAGG
Cuts at: 225 612 225
Size: 387 3662
EcoO109I rG'GnC_Cy
Cuts at: 1188 1188
Size: 4049
EcoRI G'AATT_C
Cuts at: 1053 1053
Size: 4049
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 202 2068 202
Size: 1866 2183
FspI TGC'GCA
Cuts at: 1830 1830
Size: 4049
HaeII r_GCGC'y
Cuts at: 1483 1731 2753 3732 1483
Size: 248 1022 979 1800
Fragments arranged by size:
1800 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 413 445 1891 1921 1923 1953 413
Size: 32 1446 30 2 30 2509
Fragments arranged by size:
2509 1446 32 30 30 2
HincII GTy'rAC
Cuts at: 570 707 1088 570
Size: 137 381 3531
Fragments arranged by size:
3531 381 137
HpaI GTT'AAC
Cuts at: 570 707 570
Size: 137 3912
KasI G'GCGC_C
Cuts at: 1479 1727 1479
Size: 248 3801
KpnI G_GTAC'C
Cuts at: 869 1069 869
Size: 200 3849
MfeI C'AATT_G
Cuts at: 335 335
Size: 4049
MluI A'CGCG_T
Cuts at: 3482 3482
Size: 4049
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 2720 2904 2720
Size: 184 3865
MscI TGG'CCA
Cuts at: 481 733 1810 481
Size: 252 1077 2720
Fragments arranged by size:
2720 1077 252
MslI CAynn'nnrTG
Cuts at: 280 685 727 1024 2038 2165 280
Size: 405 42 297 1014 127 2164
Fragments arranged by size:
2164 1014 405 297 127 42
MspA1I CmG'CkG
Cuts at: 299 1160 1474 1834 2847 3092 299
Size: 861 314 360 1013 245 1256
Fragments arranged by size:
1256 1013 861 360 314 245
NaeI GCC'GGC
Cuts at: 2230 2230
Size: 4049
NarI GG'CG_CC
Cuts at: 1480 1728 1480
Size: 248 3801
NcoI C'CATG_G
Cuts at: 107 728 107
Size: 621 3428
NgoMIV G'CCGG_C
Cuts at: 2228 2228
Size: 4049
NheI G'CTAG_C
Cuts at: 778 778
Size: 4049
NspI r_CATG'y
Cuts at: 1102 1602 2509 1102
Size: 500 907 2642
Fragments arranged by size:
2642 907 500
PciI A'CATG_T
Cuts at: 2505 2505
Size: 4049
PmeI GTTT'AAAC
Cuts at: 1048 1048
Size: 4049
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3188 3220 3188
Size: 32 4017
PstI C_TGCA'G
Cuts at: 1096 1781 1096
Size: 685 3364
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4049
PvuII CAG'CTG
Cuts at: 299 1474 1834 299
Size: 1175 360 2514
Fragments arranged by size:
2514 1175 360
RsrII CG'GwC_CG
Cuts at: 2244 2244
Size: 4049
SacI G_AGCT'C
Cuts at: 1063 1063
Size: 4049
SalI G'TCGA_C
Cuts at: 1086 1086
Size: 4049
SapI GCTCTTCn'nnn_
Cuts at: 2072 2282 2072
Size: 210 3839
SbfI CC_TGCA'GG
Cuts at: 1096 1096
Size: 4049
ScaI AGT'ACT
Cuts at: 3566 3566
Size: 4049
SfcI C'TryA_G
Cuts at: 33 506 1092 1777 2770 2961 33
Size: 473 586 685 993 191 1121
Fragments arranged by size:
1121 993 685 586 473 191
SfoI GGC'GCC
Cuts at: 1481 1729 1481
Size: 248 3801
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4049
SmaI CCC'GGG
Cuts at: 1071 1071
Size: 4049
SmlI C'TyrA_G
Cuts at: 594 1204 2119 2611 2873 594
Size: 610 915 492 262 1770
Fragments arranged by size:
1770 915 610 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4049
SphI G_CATG'C
Cuts at: 1102 1602 1102
Size: 500 3549
StyI C'CwwG_G
Cuts at: 107 728 1183 1537 107
Size: 621 455 354 2619
Fragments arranged by size:
2619 621 455 354
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 294 357 1632 1765 2224 2433 294
Size: 63 1275 133 459 209 1910
Fragments arranged by size:
1910 1275 459 209 133 63
TatI w'GTAC_w
Cuts at: 284 3564 284
Size: 3280 769
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3566 3598 3566
Size: 32 4017
Tth111I GACn'n_nGTC
Cuts at: 1846 1846
Size: 4049
XbaI T'CTAG_A
Cuts at: 1080 1080
Size: 4049
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1544 1544
Size: 4049
XmaI C'CCGG_G
Cuts at: 1069 1069
Size: 4049
XmnI GAAnn'nnTTC
Cuts at: 1033 1033
Size: 4049
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AgeI AlwNI ApaLI
ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI
BciVI BclI BglII BlpI Bme1580I BmrI BmtI BplI
BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BseRI BseYI
BsiEI BsiHKAI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI
BsrFI BssSI BstAPI BstBI BstXI BstYI Bsu36I BtgI
BtgZI BtsI DraI DrdI EaeI EagI EarI EciI
EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI FspI HaeII
Hin4I HincII HpaI KasI KpnI MfeI MluI MmeI
MscI MslI MspA1I NaeI NarI NcoI NgoMIV NheI
NspI PciI PmeI PpiI PstI PvuI PvuII RsrII
SacI SalI SapI SbfI ScaI SfcI SfoI SgfI
SmaI SmlI SpeI SphI StyI TaqII TatI TstI
Tth111I XbaI XcmI XmaI XmnI
Enzymes that do not cut:
AarI AatII AfeI AflII AhdI AleI AlfI AloI
ApaI AscI AvrII BaeI BbsI BbvCI BcgI BfrBI
BglI BmgBI BpmI Bpu10I BsaXI BsgI BsiWI BsmI
BsmBI BsrGI BssHII BstEII BstZ17I ClaI CspCI DraIII
EcoRV FseI FspAI HindIII NdeI NotI NruI NsiI
PacI PasI PflMI PfoI PmlI PpuMI PshAI PsiI
PspOMI PspXI PsrI SacII SanDI SexAI SfiI SgrAI
SnaBI SrfI SspI StuI SwaI XhoI ZraI