Restriction map for
pF1KE0284
(Circular) (Six-base) MAPSORT of: pF1KE0284.seq Check: 8904 from: 1 to: 4124
pF1KE0284 4124 bp
With 182 enzymes: SgfI *
April 24, 2009 13:12 ..
AccI GT'mk_AC
Cuts at: 652 1162 652
Size: 510 3614
Acc65I G'GTAC_C
Cuts at: 1140 1140
Size: 4124
AclI AA'CG_TT
Cuts at: 3964 3964
Size: 4124
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 257 1949 2381 3128 3396 257
Size: 1692 432 747 268 985
Fragments arranged by size:
1692 985 747 432 268
AflIII A'CryG_T
Cuts at: 662 1476 2580 3557 662
Size: 814 1104 977 1229
Fragments arranged by size:
1229 1104 977 814
AgeI A'CCGG_T
Cuts at: 2512 2512
Size: 4124
AlwNI CAG_nnn'CTG
Cuts at: 1429 2996 1429
Size: 1567 2557
ApaI G_GGCC'C
Cuts at: 220 220
Size: 4124
ApaLI G'TGCA_C
Cuts at: 2894 2894
Size: 4124
ApoI r'AATT_y
Cuts at: 1128 3477 3733 1128
Size: 2349 256 1519
Fragments arranged by size:
2349 1519 256
AseI AT'TA_AT
Cuts at: 20 926 20
Size: 906 3218
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4124
AvaI C'yCGr_G
Cuts at: 219 238 1144 219
Size: 19 906 3199
Fragments arranged by size:
3199 906 19
BamHI G'GATC_C
Cuts at: 1149 1149
Size: 4124
BanI G'GyrC_C
Cuts at: 1140 1554 1802 1837 1140
Size: 414 248 35 3427
Fragments arranged by size:
3427 414 248 35
BanII G_rGCy'C
Cuts at: 220 418 716 1138 2168 220
Size: 198 298 422 1030 2176
Fragments arranged by size:
2176 1030 422 298 198
BbeI G_GCGC'C
Cuts at: 1558 1806 1558
Size: 248 3876
BbsI GAAGACnn'nnnn_
Cuts at: 1075 1075
Size: 4124
BbvCI CC'TCA_GC
Cuts at: 1000 1000
Size: 4124
BciVI GTATCCnnnnn_n'
Cuts at: 2022 2783 4124 2022
Size: 761 1341 2022
Fragments arranged by size:
2022 1341 761
BclI T'GATC_A
Cuts at: 1644 1644
Size: 4124
BglII A'GATC_T
Cuts at: 1639 3676 1639
Size: 2037 2087
BlpI GC'TnA_GC
Cuts at: 1236 1236
Size: 4124
Bme1580I G_kGCm'C
Cuts at: 220 1749 1842 2898 220
Size: 1529 93 1056 1446
Fragments arranged by size:
1529 1446 1056 93
BmrI ACTGGGnnnn_n'
Cuts at: 858 1336 1417 1752 3896 858
Size: 478 81 335 2144 1086
Fragments arranged by size:
2144 1086 478 335 81
BmtI G_CTAG'C
Cuts at: 349 349
Size: 4124
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 471 2491 471
Size: 2020 2104
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 383 790 383
Size: 407 3717
Bpu10I CC'TnA_GC
Cuts at: 1000 1000
Size: 4124
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1300 2179 2671 2969 1300
Size: 879 492 298 2455
Fragments arranged by size:
2455 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 174 3828 174
Size: 3654 470
BsaAI yAC'GTr
Cuts at: 569 1477 569
Size: 908 3216
BsaBI GATnn'nnATC
Cuts at: 6 1638 3675 6
Size: 1632 2037 455
Fragments arranged by size:
2037 1632 455
BsaHI Gr'CG_yC
Cuts at: 1555 1803 2505 4001 1555
Size: 248 702 1496 1678
Fragments arranged by size:
1678 1496 702 248
BsaWI w'CCGG_w
Cuts at: 444 1313 1834 2512 2786 2933 3344 444
Size: 869 521 678 274 147 411 1224
Fragments arranged by size:
1224 869 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 105 135 152 182 634 664 105
Size: 30 17 30 452 30 3565
Fragments arranged by size:
3565 452 30 30 30 17
BseYI C'CCAG_C
Cuts at: 282 411 1549 2307 2884 3380 282
Size: 129 1138 758 577 496 1026
Fragments arranged by size:
1138 1026 758 577 496 129
BsiEI CG_ry'CG
Cuts at: 104 1666 1712 2920 104
Size: 1562 46 1208 1308
Fragments arranged by size:
1562 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 716 1138 1916 2106 2898 716
Size: 422 778 190 792 1942
Fragments arranged by size:
1942 792 778 422 190
Bsp1286I G_dGCh'C
Cuts at: 220 418 716 1138 1749 1842 1916 2106 2168
Size: 198 298 422 611 93 74 190 62
Cuts at: 2168 2898 220
Size: 730 1446
Fragments arranged by size:
1446 730 611 422 298 198 190 93
74 62
BspEI T'CCGG_A
Cuts at: 444 3344 444
Size: 2900 1224
BspHI T'CATG_A
Cuts at: 1064 3300 1064
Size: 2236 1888
BspMI ACCTGCnnnn'nnnn_
Cuts at: 1174 1388 1690 2071 1174
Size: 214 302 381 3227
Fragments arranged by size:
3227 381 302 214
BsrBI CCG'CTC
Cuts at: 50 2416 2470 3954 4119 50
Size: 2366 54 1484 165 55
Fragments arranged by size:
2366 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 515 1052 2036 515
Size: 537 984 2603
Fragments arranged by size:
2603 984 537
BsrFI r'CCGG_y
Cuts at: 289 1019 2122 2303 2512 289
Size: 730 1103 181 209 1901
Fragments arranged by size:
1901 1103 730 209 181
BsrGI T'GTAC_A
Cuts at: 706 706
Size: 4124
BssSI C'ACGA_G
Cuts at: 2395 2753 2395
Size: 358 3766
BstAPI GCAn_nnn'nTGC
Cuts at: 765 1501 765
Size: 736 3388
BstBI TT'CG_AA
Cuts at: 305 2485 305
Size: 2180 1944
BstXI CCAn_nnnn'nTGG
Cuts at: 474 490 474
Size: 16 4108
BstYI r'GATC_y
Cuts at: 1 810 1149 1616 1639 1974 2220 3232 3318
Size: 809 339 467 23 335 246 1012 86
Cuts at: 3318 3330 3676 1
Size: 12 346 449
Fragments arranged by size:
1012 809 467 449 346 339 335 246
86 23 12
Bsu36I CC'TnA_GG
Cuts at: 593 593
Size: 4124
BtgI C'CryG_G
Cuts at: 107 483 500 638 734 953 107
Size: 376 17 138 96 219 3278
Fragments arranged by size:
3278 376 219 138 96 17
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 2074 2255 2418 2074
Size: 181 163 3780
Fragments arranged by size:
3780 181 163
BtsI GCAGTG_nn'
Cuts at: 925 1367 925
Size: 442 3682
DraI TTT'AAA
Cuts at: 1123 1123
Size: 4124
DrdI GACnn_nn'nnGTC
Cuts at: 859 1830 2688 859
Size: 971 858 2295
Fragments arranged by size:
2295 971 858
EaeI y'GGCC_r
Cuts at: 630 1709 1883 2274 2301 3646 630
Size: 1079 174 391 27 1345 1108
Fragments arranged by size:
1345 1108 1079 391 174 27
EagI C'GGCC_G
Cuts at: 1709 1709
Size: 4124
EarI CTCTTCn'nnn_
Cuts at: 2147 2357 2147
Size: 210 3914
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 919 2332 2642 2788 3934 919
Size: 1413 310 146 1146 1109
Fragments arranged by size:
1413 1146 1109 310 146
EcoICRI GAG'CTC
Cuts at: 714 1136 714
Size: 422 3702
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 257 383 790 1949 2381 3128 3396 257
Size: 126 407 1159 432 747 268 985
Fragments arranged by size:
1159 985 747 432 407 268 126
EcoO109I rG'GnC_Cy
Cuts at: 996 1263 996
Size: 267 3857
EcoRI G'AATT_C
Cuts at: 1128 1128
Size: 4124
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2143 2143
Size: 4124
FseI GG_CCGG'CC
Cuts at: 1023 1023
Size: 4124
FspI TGC'GCA
Cuts at: 1905 1905
Size: 4124
HaeII r_GCGC'y
Cuts at: 1558 1806 2828 3807 1558
Size: 248 1022 979 1875
Fragments arranged by size:
1875 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 105 137 152 184 576 608 634 666 1076
Size: 32 15 32 392 32 26 32 410
Cuts at: 1076 1108 1966 1996 1998 2028 105
Size: 32 858 30 2 30 2201
Fragments arranged by size:
2201 858 410 392 32 32 32 32
32 30 30 26 15 2
HincII GTy'rAC
Cuts at: 1163 1163
Size: 4124
KasI G'GCGC_C
Cuts at: 1554 1802 1554
Size: 248 3876
KpnI G_GTAC'C
Cuts at: 1144 1144
Size: 4124
MluI A'CGCG_T
Cuts at: 3557 3557
Size: 4124
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 2795 2979 2795
Size: 184 3940
MscI TGG'CCA
Cuts at: 1885 1885
Size: 4124
MslI CAynn'nnrTG
Cuts at: 665 2113 2240 665
Size: 1448 127 2549
Fragments arranged by size:
2549 1448 127
MspA1I CmG'CkG
Cuts at: 127 286 1031 1235 1549 1909 2922 3167 127
Size: 159 745 204 314 360 1013 245 1084
Fragments arranged by size:
1084 1013 745 360 314 245 204 159
NaeI GCC'GGC
Cuts at: 1021 2305 1021
Size: 1284 2840
NarI GG'CG_CC
Cuts at: 1555 1803 1555
Size: 248 3876
NcoI C'CATG_G
Cuts at: 107 500 638 734 953 107
Size: 393 138 96 219 3278
Fragments arranged by size:
3278 393 219 138 96
NgoMIV G'CCGG_C
Cuts at: 1019 2303 1019
Size: 1284 2840
NheI G'CTAG_C
Cuts at: 345 345
Size: 4124
NspI r_CATG'y
Cuts at: 666 1177 1677 2584 666
Size: 511 500 907 2206
Fragments arranged by size:
2206 907 511 500
PasI CC'CwG_GG
Cuts at: 152 152
Size: 4124
PciI A'CATG_T
Cuts at: 662 2580 662
Size: 1918 2206
PflMI CCAn_nnn'nTGG
Cuts at: 888 888
Size: 4124
PmeI GTTT'AAAC
Cuts at: 1123 1123
Size: 4124
PmlI CAC'GTG
Cuts at: 569 569
Size: 4124
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3263 3295 3263
Size: 32 4092
PspOMI G'GGCC_C
Cuts at: 216 216
Size: 4124
PstI C_TGCA'G
Cuts at: 761 1171 1856 761
Size: 410 685 3029
Fragments arranged by size:
3029 685 410
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4124
PvuII CAG'CTG
Cuts at: 286 1549 1909 286
Size: 1263 360 2501
Fragments arranged by size:
2501 1263 360
RsrII CG'GwC_CG
Cuts at: 2319 2319
Size: 4124
SacI G_AGCT'C
Cuts at: 716 1138 716
Size: 422 3702
SalI G'TCGA_C
Cuts at: 1161 1161
Size: 4124
SapI GCTCTTCn'nnn_
Cuts at: 2147 2357 2147
Size: 210 3914
SbfI CC_TGCA'GG
Cuts at: 1171 1171
Size: 4124
ScaI AGT'ACT
Cuts at: 886 3641 886
Size: 2755 1369
SfcI C'TryA_G
Cuts at: 33 677 757 1167 1852 2845 3036 33
Size: 644 80 410 685 993 191 1121
Fragments arranged by size:
1121 993 685 644 410 191 80
SfoI GGC'GCC
Cuts at: 1556 1804 1556
Size: 248 3876
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4124
SmaI CCC'GGG
Cuts at: 240 1146 240
Size: 906 3218
SmlI C'TyrA_G
Cuts at: 1279 2194 2686 2948 1279
Size: 915 492 262 2455
Fragments arranged by size:
2455 915 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4124
SphI G_CATG'C
Cuts at: 1177 1677 1177
Size: 500 3624
StuI AGG'CCT
Cuts at: 803 803
Size: 4124
StyI C'CwwG_G
Cuts at: 107 500 638 734 953 1258 1612 107
Size: 393 138 96 219 305 354 2619
Fragments arranged by size:
2619 393 354 305 219 138 96
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1707 1840 2299 2508 1707
Size: 133 459 209 3323
Fragments arranged by size:
3323 459 209 133
TatI w'GTAC_w
Cuts at: 706 884 3639 706
Size: 178 2755 1191
Fragments arranged by size:
2755 1191 178
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3641 3673 3641
Size: 32 4092
Tth111I GACn'n_nGTC
Cuts at: 1921 1921
Size: 4124
XbaI T'CTAG_A
Cuts at: 981 1155 981
Size: 174 3950
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 471 507 1619 471
Size: 36 1112 2976
Fragments arranged by size:
2976 1112 36
XmaI C'CCGG_G
Cuts at: 238 1144 238
Size: 906 3218
XmnI GAAnn'nnTTC
Cuts at: 1114 1114
Size: 4124
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AgeI AlwNI ApaI
ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII
BbeI BbsI BbvCI BciVI BclI BglII BlpI Bme1580I
BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI
BsaBI BsaHI BsaWI BsaXI BseYI BsiEI BsiHKAI Bsp1286I
BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssSI
BstAPI BstBI BstXI BstYI Bsu36I BtgI BtgZI BtsI
DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI
EcoO109I EcoRI FalI FseI FspI HaeII Hin4I HincII
KasI KpnI MluI MmeI MscI MslI MspA1I NaeI
NarI NcoI NgoMIV NheI NspI PasI PciI PflMI
PmeI PmlI PpiI PspOMI PstI PvuI PvuII RsrII
SacI SalI SapI SbfI ScaI SfcI SfoI SgfI
SmaI SmlI SpeI SphI StuI StyI TaqII TatI
TstI Tth111I XbaI XcmI XmaI XmnI
Enzymes that do not cut:
AarI AatII AfeI AflII AhdI AleI AlfI AloI
AscI AvrII BaeI BcgI BfrBI BglI BmgBI BseRI
BsgI BsiWI BsmI BsmBI BssHII BstEII BstZ17I ClaI
CspCI DraIII EcoNI EcoRV FspAI HindIII HpaI MfeI
NdeI NotI NruI NsiI PacI PfoI PpuMI PshAI
PsiI PspXI PsrI SacII SanDI SexAI SfiI SgrAI
SnaBI SrfI SspI SwaI XhoI ZraI