Restriction map for
pF1KE0281
(Circular) (Six-base) MAPSORT of: pF1KE0281.seq Check: 2056 from: 1 to: 3815
pF1KE0281 3815 bp
With 182 enzymes: SgfI *
April 24, 2009 13:12 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 262 478 655 262
Size: 216 177 3422
Fragments arranged by size:
3422 216 177
AccI GT'mk_AC
Cuts at: 853 853
Size: 3815
Acc65I G'GTAC_C
Cuts at: 831 831
Size: 3815
AclI AA'CG_TT
Cuts at: 3655 3655
Size: 3815
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 387 1640 2072 2819 3087 387
Size: 1253 432 747 268 1115
Fragments arranged by size:
1253 1115 747 432 268
AflIII A'CryG_T
Cuts at: 1167 2271 3248 1167
Size: 1104 977 1734
Fragments arranged by size:
1734 1104 977
AgeI A'CCGG_T
Cuts at: 2203 2203
Size: 3815
AleI CACnn'nnGTG
Cuts at: 507 507
Size: 3815
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 331 663 331
Size: 332 3483
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 232 264 232
Size: 32 3783
AlwNI CAG_nnn'CTG
Cuts at: 1120 2687 1120
Size: 1567 2248
ApaLI G'TGCA_C
Cuts at: 2585 2585
Size: 3815
ApoI r'AATT_y
Cuts at: 819 3168 3424 819
Size: 2349 256 1210
Fragments arranged by size:
2349 1210 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 3815
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 3815
AvaI C'yCGr_G
Cuts at: 835 835
Size: 3815
BamHI G'GATC_C
Cuts at: 840 840
Size: 3815
BanI G'GyrC_C
Cuts at: 114 740 831 1245 1493 1528 114
Size: 626 91 414 248 35 2401
Fragments arranged by size:
2401 626 414 248 91 35
BanII G_rGCy'C
Cuts at: 553 712 829 1859 553
Size: 159 117 1030 2509
Fragments arranged by size:
2509 1030 159 117
BbeI G_GCGC'C
Cuts at: 118 744 1249 1497 118
Size: 626 505 248 2436
Fragments arranged by size:
2436 626 505 248
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 90 124 90
Size: 34 3781
BciVI GTATCCnnnnn_n'
Cuts at: 1713 2474 3815 1713
Size: 761 1341 1713
Fragments arranged by size:
1713 1341 761
BclI T'GATC_A
Cuts at: 1335 1335
Size: 3815
BglI GCCn_nnn'nGGC
Cuts at: 112 203 290 305 479 797 112
Size: 91 87 15 174 318 3130
Fragments arranged by size:
3130 318 174 91 87 15
BglII A'GATC_T
Cuts at: 1330 3367 1330
Size: 2037 1778
BlpI GC'TnA_GC
Cuts at: 927 927
Size: 3815
Bme1580I G_kGCm'C
Cuts at: 193 484 1440 1533 2589 193
Size: 291 956 93 1056 1419
Fragments arranged by size:
1419 1056 956 291 93
BmgBI CAC'GTC
Cuts at: 780 780
Size: 3815
BmrI ACTGGGnnnn_n'
Cuts at: 538 1027 1108 1443 3587 538
Size: 489 81 335 2144 766
Fragments arranged by size:
2144 766 489 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2182 2182
Size: 3815
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 567 616 567
Size: 49 3766
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 991 1870 2362 2660 991
Size: 879 492 298 2146
Fragments arranged by size:
2146 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 3519 3519
Size: 3815
BsaAI yAC'GTr
Cuts at: 1168 1168
Size: 3815
BsaBI GATnn'nnATC
Cuts at: 6 1329 3366 6
Size: 1323 2037 455
Fragments arranged by size:
2037 1323 455
BsaHI Gr'CG_yC
Cuts at: 115 741 1246 1494 2196 3692 115
Size: 626 505 248 702 1496 238
Fragments arranged by size:
1496 702 626 505 248 238
BsaWI w'CCGG_w
Cuts at: 422 1004 1525 2203 2477 2624 3035 422
Size: 582 521 678 274 147 411 1202
Fragments arranged by size:
1202 678 582 521 411 274 147
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 250 649 720 250
Size: 399 71 3345
Fragments arranged by size:
3345 399 71
BseYI C'CCAG_C
Cuts at: 625 1240 1998 2575 3071 625
Size: 615 758 577 496 1369
Fragments arranged by size:
1369 758 615 577 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 195 301 540 195
Size: 106 239 3470
Fragments arranged by size:
3470 239 106
BsiEI CG_ry'CG
Cuts at: 104 1357 1403 2611 104
Size: 1253 46 1208 1308
Fragments arranged by size:
1308 1253 1208 46
BsiHKAI G_wGCw'C
Cuts at: 829 1607 1797 2589 829
Size: 778 190 792 2055
Fragments arranged by size:
2055 792 778 190
Bsp1286I G_dGCh'C
Cuts at: 193 484 553 712 829 1440 1533 1607 1797
Size: 291 69 159 117 611 93 74 190
Cuts at: 1797 1859 2589 193
Size: 62 730 1419
Fragments arranged by size:
1419 730 611 291 190 159 117 93
74 69 62
BspEI T'CCGG_A
Cuts at: 3035 3035
Size: 3815
BspHI T'CATG_A
Cuts at: 2991 2991
Size: 3815
BspMI ACCTGCnnnn'nnnn_
Cuts at: 262 478 583 655 865 1079 1381 1762 262
Size: 216 105 72 210 214 302 381 2315
Fragments arranged by size:
2315 381 302 216 214 210 105 72
BsrBI CCG'CTC
Cuts at: 50 2107 2161 3645 3810 50
Size: 2057 54 1484 165 55
Fragments arranged by size:
2057 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 1727 1727
Size: 3815
BsrFI r'CCGG_y
Cuts at: 737 1813 1994 2203 737
Size: 1076 181 209 2349
Fragments arranged by size:
2349 1076 209 181
BsrGI T'GTAC_A
Cuts at: 446 446
Size: 3815
BssSI C'ACGA_G
Cuts at: 2086 2444 2086
Size: 358 3457
BstAPI GCAn_nnn'nTGC
Cuts at: 425 1192 425
Size: 767 3048
BstBI TT'CG_AA
Cuts at: 2176 2176
Size: 3815
BstYI r'GATC_y
Cuts at: 1 840 1307 1330 1665 1911 2923 3009 3021
Size: 839 467 23 335 246 1012 86 12
Cuts at: 3021 3367 1
Size: 346 449
Fragments arranged by size:
1012 839 467 449 346 335 246 86
23 12
BtgI C'CryG_G
Cuts at: 149 348 149
Size: 199 3616
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 1765 1946 2109 1765
Size: 181 163 3471
Fragments arranged by size:
3471 181 163
BtsI GCAGTG_nn'
Cuts at: 1058 1058
Size: 3815
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 360 395 360
Size: 35 3780
DraI TTT'AAA
Cuts at: 814 814
Size: 3815
DraIII CAC_nnn'GTG
Cuts at: 150 150
Size: 3815
DrdI GACnn_nn'nnGTC
Cuts at: 1521 2379 1521
Size: 858 2957
EaeI y'GGCC_r
Cuts at: 351 618 714 1400 1574 1965 1992 3337 351
Size: 267 96 686 174 391 27 1345 829
Fragments arranged by size:
1345 829 686 391 267 174 96 27
EagI C'GGCC_G
Cuts at: 1400 1400
Size: 3815
EarI CTCTTCn'nnn_
Cuts at: 274 1838 2048 274
Size: 1564 210 2041
Fragments arranged by size:
2041 1564 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 271 286 2023 2333 2479 3625 271
Size: 15 1737 310 146 1146 461
Fragments arranged by size:
1737 1146 461 310 146 15
EcoICRI GAG'CTC
Cuts at: 827 827
Size: 3815
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 387 567 616 1640 2072 2819 3087 387
Size: 180 49 1024 432 747 268 1115
Fragments arranged by size:
1115 1024 747 432 268 180 49
EcoO109I rG'GnC_Cy
Cuts at: 532 591 689 954 532
Size: 59 98 265 3393
Fragments arranged by size:
3393 265 98 59
EcoRI G'AATT_C
Cuts at: 819 819
Size: 3815
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 1834 1834
Size: 3815
FspI TGC'GCA
Cuts at: 1596 1596
Size: 3815
HaeII r_GCGC'y
Cuts at: 118 744 1249 1497 2519 3498 118
Size: 626 505 248 1022 979 435
Fragments arranged by size:
1022 979 626 505 435 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1657 1687 1689 1719 1657
Size: 30 2 30 3753
Fragments arranged by size:
3753 30 30 2
HincII GTy'rAC
Cuts at: 363 854 363
Size: 491 3324
KasI G'GCGC_C
Cuts at: 114 740 1245 1493 114
Size: 626 505 248 2436
Fragments arranged by size:
2436 626 505 248
KpnI G_GTAC'C
Cuts at: 835 835
Size: 3815
MluI A'CGCG_T
Cuts at: 3248 3248
Size: 3815
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 2486 2670 2486
Size: 184 3631
MscI TGG'CCA
Cuts at: 1576 1576
Size: 3815
MslI CAynn'nnrTG
Cuts at: 507 1804 1931 507
Size: 1297 127 2391
Fragments arranged by size:
2391 1297 127
MspA1I CmG'CkG
Cuts at: 350 926 1240 1600 2613 2858 350
Size: 576 314 360 1013 245 1307
Fragments arranged by size:
1307 1013 576 360 314 245
NaeI GCC'GGC
Cuts at: 1996 1996
Size: 3815
NarI GG'CG_CC
Cuts at: 115 741 1246 1494 115
Size: 626 505 248 2436
Fragments arranged by size:
2436 626 505 248
NgoMIV G'CCGG_C
Cuts at: 1994 1994
Size: 3815
NspI r_CATG'y
Cuts at: 159 868 1368 2275 159
Size: 709 500 907 1699
Fragments arranged by size:
1699 907 709 500
PasI CC'CwG_GG
Cuts at: 331 536 331
Size: 205 3610
PciI A'CATG_T
Cuts at: 2271 2271
Size: 3815
PflMI CCAn_nnn'nTGG
Cuts at: 150 150
Size: 3815
PmeI GTTT'AAAC
Cuts at: 814 814
Size: 3815
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2954 2986 2954
Size: 32 3783
PpuMI rG'GwC_Cy
Cuts at: 532 532
Size: 3815
PstI C_TGCA'G
Cuts at: 806 862 1547 806
Size: 56 685 3074
Fragments arranged by size:
3074 685 56
PvuI CG_AT'CG
Cuts at: 104 104
Size: 3815
PvuII CAG'CTG
Cuts at: 1240 1600 1240
Size: 360 3455
RsrII CG'GwC_CG
Cuts at: 2010 2010
Size: 3815
SacI G_AGCT'C
Cuts at: 829 829
Size: 3815
SacII CC_GC'GG
Cuts at: 351 351
Size: 3815
SalI G'TCGA_C
Cuts at: 852 852
Size: 3815
SanDI GG'GwC_CC
Cuts at: 532 532
Size: 3815
SapI GCTCTTCn'nnn_
Cuts at: 274 1838 2048 274
Size: 1564 210 2041
Fragments arranged by size:
2041 1564 210
SbfI CC_TGCA'GG
Cuts at: 862 862
Size: 3815
ScaI AGT'ACT
Cuts at: 3332 3332
Size: 3815
SfcI C'TryA_G
Cuts at: 33 802 858 1543 2536 2727 33
Size: 769 56 685 993 191 1121
Fragments arranged by size:
1121 993 769 685 191 56
SfoI GGC'GCC
Cuts at: 116 742 1247 1495 116
Size: 626 505 248 2436
Fragments arranged by size:
2436 626 505 248
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 3815
SmaI CCC'GGG
Cuts at: 837 837
Size: 3815
SmlI C'TyrA_G
Cuts at: 970 1885 2377 2639 970
Size: 915 492 262 2146
Fragments arranged by size:
2146 915 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 3815
SphI G_CATG'C
Cuts at: 868 1368 868
Size: 500 3315
StyI C'CwwG_G
Cuts at: 949 1303 949
Size: 354 3461
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1398 1531 1990 2199 1398
Size: 133 459 209 3014
Fragments arranged by size:
3014 459 209 133
TatI w'GTAC_w
Cuts at: 446 3330 446
Size: 2884 931
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3332 3364 3332
Size: 32 3783
Tth111I GACn'n_nGTC
Cuts at: 1612 1612
Size: 3815
XbaI T'CTAG_A
Cuts at: 846 846
Size: 3815
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1310 1310
Size: 3815
XmaI C'CCGG_G
Cuts at: 835 835
Size: 3815
Enzymes that do cut:
AarI AccI Acc65I AclI AcuI AflIII AgeI AleI
AlfI AloI AlwNI ApaLI ApoI AseI AsiSI AvaI
BamHI BanI BanII BbeI BcgI BciVI BclI BglI
BglII BlpI Bme1580I BmgBI BmrI BplI BpmI BpuEI
BsaI BsaAI BsaBI BsaHI BsaWI BseRI BseYI BsgI
BsiEI BsiHKAI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI
BsrFI BsrGI BssSI BstAPI BstBI BstYI BtgI BtgZI
BtsI CspCI DraI DraIII DrdI EaeI EagI EarI
EciI EcoICRI Eco57MI EcoO109I EcoRI FalI FspI HaeII
Hin4I HincII KasI KpnI MluI MmeI MscI MslI
MspA1I NaeI NarI NgoMIV NspI PasI PciI PflMI
PmeI PpiI PpuMI PstI PvuI PvuII RsrII SacI
SacII SalI SanDI SapI SbfI ScaI SfcI SfoI
SgfI SmaI SmlI SpeI SphI StyI TaqII TatI
TstI Tth111I XbaI XcmI XmaI
Enzymes that do not cut:
AatII AfeI AflII AhdI ApaI AscI AvrII BaeI
BbsI BbvCI BfrBI BmtI Bpu10I BsaXI BsiWI BsmI
BsmBI BssHII BstEII BstXI BstZ17I Bsu36I ClaI EcoNI
EcoRV FseI FspAI HindIII HpaI MfeI NcoI NdeI
NheI NotI NruI NsiI PacI PfoI PmlI PshAI
PsiI PspOMI PspXI PsrI SexAI SfiI SgrAI SnaBI
SrfI SspI StuI SwaI XhoI XmnI ZraI