Restriction map for
pF1KE0275
(Circular) (Six-base) MAPSORT of: pF1KE0275.seq Check: 7395 from: 1 to: 3635
pF1KE0275 3635 bp
With 182 enzymes: SgfI *
April 24, 2009 13:12 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 339 339
Size: 3635
AccI GT'mk_AC
Cuts at: 673 673
Size: 3635
Acc65I G'GTAC_C
Cuts at: 651 651
Size: 3635
AclI AA'CG_TT
Cuts at: 3475 3475
Size: 3635
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1460 1892 2639 2907 1460
Size: 432 747 268 2188
Fragments arranged by size:
2188 747 432 268
AfeI AGC'GCT
Cuts at: 407 407
Size: 3635
AflIII A'CryG_T
Cuts at: 987 2091 3068 987
Size: 1104 977 1554
Fragments arranged by size:
1554 1104 977
AgeI A'CCGG_T
Cuts at: 2023 2023
Size: 3635
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 565 565
Size: 3635
AlwNI CAG_nnn'CTG
Cuts at: 940 2507 940
Size: 1567 2068
ApaLI G'TGCA_C
Cuts at: 2405 2405
Size: 3635
ApoI r'AATT_y
Cuts at: 639 2988 3244 639
Size: 2349 256 1030
Fragments arranged by size:
2349 1030 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 3635
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 3635
AvaI C'yCGr_G
Cuts at: 655 655
Size: 3635
BamHI G'GATC_C
Cuts at: 660 660
Size: 3635
BanI G'GyrC_C
Cuts at: 651 1065 1313 1348 651
Size: 414 248 35 2938
Fragments arranged by size:
2938 414 248 35
BanII G_rGCy'C
Cuts at: 161 524 649 1679 161
Size: 363 125 1030 2117
Fragments arranged by size:
2117 1030 363 125
BbeI G_GCGC'C
Cuts at: 1069 1317 1069
Size: 248 3387
BbsI GAAGACnn'nnnn_
Cuts at: 133 133
Size: 3635
BciVI GTATCCnnnnn_n'
Cuts at: 1533 2294 3635 1533
Size: 761 1341 1533
Fragments arranged by size:
1533 1341 761
BclI T'GATC_A
Cuts at: 1155 1155
Size: 3635
BglI GCCn_nnn'nGGC
Cuts at: 503 571 503
Size: 68 3567
BglII A'GATC_T
Cuts at: 1150 3187 1150
Size: 2037 1598
BlpI GC'TnA_GC
Cuts at: 747 747
Size: 3635
Bme1580I G_kGCm'C
Cuts at: 1260 1353 2409 1260
Size: 93 1056 2486
Fragments arranged by size:
2486 1056 93
BmrI ACTGGGnnnn_n'
Cuts at: 847 928 1263 3407 847
Size: 81 335 2144 1075
Fragments arranged by size:
2144 1075 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 321 2002 321
Size: 1681 1954
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 256 493 256
Size: 237 3398
Bpu10I CC'TnA_GC
Cuts at: 402 402
Size: 3635
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 811 1690 2182 2480 811
Size: 879 492 298 1966
Fragments arranged by size:
1966 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 3339 3339
Size: 3635
BsaAI yAC'GTr
Cuts at: 988 988
Size: 3635
BsaBI GATnn'nnATC
Cuts at: 6 1149 3186 6
Size: 1143 2037 455
Fragments arranged by size:
2037 1143 455
BsaHI Gr'CG_yC
Cuts at: 1066 1314 2016 3512 1066
Size: 248 702 1496 1189
Fragments arranged by size:
1496 1189 702 248
BsaWI w'CCGG_w
Cuts at: 824 1345 2023 2297 2444 2855 824
Size: 521 678 274 147 411 1604
Fragments arranged by size:
1604 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 448 478 448
Size: 30 3605
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 274 295 472 514 274
Size: 21 177 42 3395
Fragments arranged by size:
3395 177 42 21
BseYI C'CCAG_C
Cuts at: 344 411 561 619 1060 1818 2395 2891 344
Size: 67 150 58 441 758 577 496 1088
Fragments arranged by size:
1088 758 577 496 441 150 67 58
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 535 535
Size: 3635
BsiEI CG_ry'CG
Cuts at: 104 490 1177 1223 2431 104
Size: 386 687 46 1208 1308
Fragments arranged by size:
1308 1208 687 386 46
BsiHKAI G_wGCw'C
Cuts at: 161 524 649 1427 1617 2409 161
Size: 363 125 778 190 792 1387
Fragments arranged by size:
1387 792 778 363 190 125
Bsp1286I G_dGCh'C
Cuts at: 161 524 649 1260 1353 1427 1617 1679 2409
Size: 363 125 611 93 74 190 62 730
Cuts at: 2409 161
Size: 1387
Fragments arranged by size:
1387 730 611 363 190 125 93 74
62
BspEI T'CCGG_A
Cuts at: 2855 2855
Size: 3635
BspHI T'CATG_A
Cuts at: 2811 2811
Size: 3635
BspMI ACCTGCnnnn'nnnn_
Cuts at: 339 685 899 1201 1582 339
Size: 346 214 302 381 2392
Fragments arranged by size:
2392 381 346 302 214
BsrBI CCG'CTC
Cuts at: 50 300 1927 1981 3465 3630 50
Size: 250 1627 54 1484 165 55
Fragments arranged by size:
1627 1484 250 165 55 54
BsrDI GCAATG_nn'
Cuts at: 1547 1547
Size: 3635
BsrFI r'CCGG_y
Cuts at: 1633 1814 2023 1633
Size: 181 209 3245
Fragments arranged by size:
3245 209 181
BssHII G'CGCG_C
Cuts at: 366 366
Size: 3635
BssSI C'ACGA_G
Cuts at: 1906 2264 1906
Size: 358 3277
BstAPI GCAn_nnn'nTGC
Cuts at: 539 1012 539
Size: 473 3162
BstBI TT'CG_AA
Cuts at: 1996 1996
Size: 3635
BstYI r'GATC_y
Cuts at: 1 476 597 660 1127 1150 1485 1731 2743
Size: 475 121 63 467 23 335 246 1012
Cuts at: 2743 2829 2841 3187 1
Size: 86 12 346 449
Fragments arranged by size:
1012 475 467 449 346 335 246 121
86 63 23 12
BtgI C'CryG_G
Cuts at: 107 268 498 107
Size: 161 230 3244
Fragments arranged by size:
3244 230 161
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 1585 1766 1929 1585
Size: 181 163 3291
Fragments arranged by size:
3291 181 163
BtsI GCAGTG_nn'
Cuts at: 576 878 576
Size: 302 3333
DraI TTT'AAA
Cuts at: 634 634
Size: 3635
DrdI GACnn_nn'nnGTC
Cuts at: 1341 2199 1341
Size: 858 2777
EaeI y'GGCC_r
Cuts at: 1220 1394 1785 1812 3157 1220
Size: 174 391 27 1345 1698
Fragments arranged by size:
1698 1345 391 174 27
EagI C'GGCC_G
Cuts at: 1220 1220
Size: 3635
EarI CTCTTCn'nnn_
Cuts at: 149 532 1658 1868 149
Size: 383 1126 210 1916
Fragments arranged by size:
1916 1126 383 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 1843 2153 2299 3445 1843
Size: 310 146 1146 2033
Fragments arranged by size:
2033 1146 310 146
EcoICRI GAG'CTC
Cuts at: 159 522 647 159
Size: 363 125 3147
Fragments arranged by size:
3147 363 125
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 256 493 1460 1892 2639 2907 256
Size: 237 967 432 747 268 984
Fragments arranged by size:
984 967 747 432 268 237
EcoNI CCTnn'n_nnAGG
Cuts at: 454 454
Size: 3635
EcoO109I rG'GnC_Cy
Cuts at: 774 774
Size: 3635
EcoRI G'AATT_C
Cuts at: 639 639
Size: 3635
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 150 1654 150
Size: 1504 2131
FspI TGC'GCA
Cuts at: 1416 1416
Size: 3635
HaeII r_GCGC'y
Cuts at: 306 358 409 1069 1317 2339 3318 306
Size: 52 51 660 248 1022 979 623
Fragments arranged by size:
1022 979 660 623 248 52 51
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1477 1507 1509 1539 1477
Size: 30 2 30 3573
Fragments arranged by size:
3573 30 30 2
HincII GTy'rAC
Cuts at: 674 674
Size: 3635
KasI G'GCGC_C
Cuts at: 1065 1313 1065
Size: 248 3387
KpnI G_GTAC'C
Cuts at: 655 655
Size: 3635
MluI A'CGCG_T
Cuts at: 3068 3068
Size: 3635
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 182 2306 2490 182
Size: 2124 184 1327
Fragments arranged by size:
2124 1327 184
MscI TGG'CCA
Cuts at: 1396 1396
Size: 3635
MslI CAynn'nnrTG
Cuts at: 1624 1751 1624
Size: 127 3508
MspA1I CmG'CkG
Cuts at: 234 270 324 377 500 619 746 1060 1420
Size: 36 54 53 123 119 127 314 360
Cuts at: 1420 2433 2678 234
Size: 1013 245 1191
Fragments arranged by size:
1191 1013 360 314 245 127 123 119
54 53 36
NaeI GCC'GGC
Cuts at: 1816 1816
Size: 3635
NarI GG'CG_CC
Cuts at: 1066 1314 1066
Size: 248 3387
NcoI C'CATG_G
Cuts at: 107 107
Size: 3635
NgoMIV G'CCGG_C
Cuts at: 1814 1814
Size: 3635
NspI r_CATG'y
Cuts at: 688 1188 2095 688
Size: 500 907 2228
Fragments arranged by size:
2228 907 500
PciI A'CATG_T
Cuts at: 2091 2091
Size: 3635
PflMI CCAn_nnn'nTGG
Cuts at: 351 351
Size: 3635
PfoI T'CCnGG_A
Cuts at: 452 452
Size: 3635
PmeI GTTT'AAAC
Cuts at: 634 634
Size: 3635
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2774 2806 2774
Size: 32 3603
PstI C_TGCA'G
Cuts at: 682 1367 682
Size: 685 2950
PvuI CG_AT'CG
Cuts at: 104 104
Size: 3635
PvuII CAG'CTG
Cuts at: 1060 1420 1060
Size: 360 3275
RsrII CG'GwC_CG
Cuts at: 1830 1830
Size: 3635
SacI G_AGCT'C
Cuts at: 161 524 649 161
Size: 363 125 3147
Fragments arranged by size:
3147 363 125
SacII CC_GC'GG
Cuts at: 271 501 271
Size: 230 3405
SalI G'TCGA_C
Cuts at: 672 672
Size: 3635
SapI GCTCTTCn'nnn_
Cuts at: 149 1658 1868 149
Size: 1509 210 1916
Fragments arranged by size:
1916 1509 210
SbfI CC_TGCA'GG
Cuts at: 682 682
Size: 3635
ScaI AGT'ACT
Cuts at: 3152 3152
Size: 3635
SfcI C'TryA_G
Cuts at: 33 678 1363 2356 2547 33
Size: 645 685 993 191 1121
Fragments arranged by size:
1121 993 685 645 191
SfoI GGC'GCC
Cuts at: 1067 1315 1067
Size: 248 3387
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 3635
SmaI CCC'GGG
Cuts at: 657 657
Size: 3635
SmlI C'TyrA_G
Cuts at: 790 1705 2197 2459 790
Size: 915 492 262 1966
Fragments arranged by size:
1966 915 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 3635
SphI G_CATG'C
Cuts at: 688 1188 688
Size: 500 3135
StyI C'CwwG_G
Cuts at: 107 769 1123 107
Size: 662 354 2619
Fragments arranged by size:
2619 662 354
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 358 575 1218 1351 1810 2019 358
Size: 217 643 133 459 209 1974
Fragments arranged by size:
1974 643 459 217 209 133
TatI w'GTAC_w
Cuts at: 3150 3150
Size: 3635
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 448 480 3152 3184 448
Size: 32 2672 32 899
Fragments arranged by size:
2672 899 32 32
Tth111I GACn'n_nGTC
Cuts at: 1432 1432
Size: 3635
XbaI T'CTAG_A
Cuts at: 666 666
Size: 3635
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1130 1130
Size: 3635
XmaI C'CCGG_G
Cuts at: 655 655
Size: 3635
Enzymes that do cut:
AarI AccI Acc65I AclI AcuI AfeI AflIII AgeI
AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI
BanI BanII BbeI BbsI BciVI BclI BglI BglII
BlpI Bme1580I BmrI BplI BpmI Bpu10I BpuEI BsaI
BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI
BsiEI BsiHKAI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI
BsrFI BssHII BssSI BstAPI BstBI BstYI BtgI BtgZI
BtsI DraI DrdI EaeI EagI EarI EciI EcoICRI
Eco57MI EcoNI EcoO109I EcoRI FalI FspI HaeII Hin4I
HincII KasI KpnI MluI MmeI MscI MslI MspA1I
NaeI NarI NcoI NgoMIV NspI PciI PflMI PfoI
PmeI PpiI PstI PvuI PvuII RsrII SacI SacII
SalI SapI SbfI ScaI SfcI SfoI SgfI SmaI
SmlI SpeI SphI StyI TaqII TatI TstI Tth111I
XbaI XcmI XmaI
Enzymes that do not cut:
AatII AflII AhdI AleI AloI ApaI AscI AvrII
BaeI BbvCI BcgI BfrBI BmgBI BmtI BsiWI BsmI
BsmBI BsrGI BstEII BstXI BstZ17I Bsu36I ClaI CspCI
DraIII EcoRV FseI FspAI HindIII HpaI MfeI NdeI
NheI NotI NruI NsiI PacI PasI PmlI PpuMI
PshAI PsiI PspOMI PspXI PsrI SanDI SexAI SfiI
SgrAI SnaBI SrfI SspI StuI SwaI XhoI XmnI
ZraI