Restriction map for
pF1KE0274
(Circular) (Six-base) MAPSORT of: pF1KE0274.seq Check: 8491 from: 1 to: 3440
pF1KE0274 3440 bp
With 182 enzymes: SgfI *
April 24, 2009 13:12 ..
AatII G_ACGT'C
Cuts at: 208 208
Size: 3440
AccI GT'mk_AC
Cuts at: 478 478
Size: 3440
Acc65I G'GTAC_C
Cuts at: 456 456
Size: 3440
AclI AA'CG_TT
Cuts at: 3280 3280
Size: 3440
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1265 1697 2444 2712 1265
Size: 432 747 268 1993
Fragments arranged by size:
1993 747 432 268
AfeI AGC'GCT
Cuts at: 152 152
Size: 3440
AflII C'TTAA_G
Cuts at: 298 298
Size: 3440
AflIII A'CryG_T
Cuts at: 792 1896 2873 792
Size: 1104 977 1359
Fragments arranged by size:
1359 1104 977
AgeI A'CCGG_T
Cuts at: 1828 1828
Size: 3440
AleI CACnn'nnGTG
Cuts at: 121 121
Size: 3440
AlwNI CAG_nnn'CTG
Cuts at: 745 2312 745
Size: 1567 1873
ApaLI G'TGCA_C
Cuts at: 2210 2210
Size: 3440
ApoI r'AATT_y
Cuts at: 193 412 444 2793 3049 193
Size: 219 32 2349 256 584
Fragments arranged by size:
2349 584 256 219 32
AseI AT'TA_AT
Cuts at: 20 20
Size: 3440
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 3440
AvaI C'yCGr_G
Cuts at: 460 460
Size: 3440
BamHI G'GATC_C
Cuts at: 465 465
Size: 3440
BanI G'GyrC_C
Cuts at: 328 456 870 1118 1153 328
Size: 128 414 248 35 2615
Fragments arranged by size:
2615 414 248 128 35
BanII G_rGCy'C
Cuts at: 454 1484 454
Size: 1030 2410
BbeI G_GCGC'C
Cuts at: 874 1122 874
Size: 248 3192
BciVI GTATCCnnnnn_n'
Cuts at: 1338 2099 3440 1338
Size: 761 1341 1338
Fragments arranged by size:
1341 1338 761
BclI T'GATC_A
Cuts at: 960 960
Size: 3440
BglII A'GATC_T
Cuts at: 955 2992 955
Size: 2037 1403
BlpI GC'TnA_GC
Cuts at: 552 552
Size: 3440
Bme1580I G_kGCm'C
Cuts at: 1065 1158 2214 1065
Size: 93 1056 2291
Fragments arranged by size:
2291 1056 93
BmgBI CAC'GTC
Cuts at: 123 123
Size: 3440
BmrI ACTGGGnnnn_n'
Cuts at: 652 733 1068 3212 652
Size: 81 335 2144 880
Fragments arranged by size:
2144 880 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 1807 1807
Size: 3440
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 139 295 139
Size: 156 3284
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 616 1495 1987 2285 616
Size: 879 492 298 1771
Fragments arranged by size:
1771 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 3144 3144
Size: 3440
BsaAI yAC'GTr
Cuts at: 793 793
Size: 3440
BsaBI GATnn'nnATC
Cuts at: 6 954 2991 6
Size: 948 2037 455
Fragments arranged by size:
2037 948 455
BsaHI Gr'CG_yC
Cuts at: 205 871 1119 1821 3317 205
Size: 666 248 702 1496 328
Fragments arranged by size:
1496 702 666 328 248
BsaWI w'CCGG_w
Cuts at: 251 629 1150 1828 2102 2249 2660 251
Size: 378 521 678 274 147 411 1031
Fragments arranged by size:
1031 678 521 411 378 274 147
BseYI C'CCAG_C
Cuts at: 865 1623 2200 2696 865
Size: 758 577 496 1609
Fragments arranged by size:
1609 758 577 496
BsiEI CG_ry'CG
Cuts at: 104 982 1028 2236 104
Size: 878 46 1208 1308
Fragments arranged by size:
1308 1208 878 46
BsiHKAI G_wGCw'C
Cuts at: 128 454 1232 1422 2214 128
Size: 326 778 190 792 1354
Fragments arranged by size:
1354 792 778 326 190
BsmI GAATG_Cn'
Cuts at: 166 429 166
Size: 263 3177
Bsp1286I G_dGCh'C
Cuts at: 128 454 1065 1158 1232 1422 1484 2214 128
Size: 326 611 93 74 190 62 730 1354
Fragments arranged by size:
1354 730 611 326 190 93 74 62
BspEI T'CCGG_A
Cuts at: 2660 2660
Size: 3440
BspHI T'CATG_A
Cuts at: 2616 2616
Size: 3440
BspMI ACCTGCnnnn'nnnn_
Cuts at: 490 704 1006 1387 490
Size: 214 302 381 2543
Fragments arranged by size:
2543 381 302 214
BsrBI CCG'CTC
Cuts at: 50 275 1732 1786 3270 3435 50
Size: 225 1457 54 1484 165 55
Fragments arranged by size:
1484 1457 225 165 55 54
BsrDI GCAATG_nn'
Cuts at: 140 1352 140
Size: 1212 2228
BsrFI r'CCGG_y
Cuts at: 1438 1619 1828 1438
Size: 181 209 3050
Fragments arranged by size:
3050 209 181
BssSI C'ACGA_G
Cuts at: 1711 2069 1711
Size: 358 3082
BstAPI GCAn_nnn'nTGC
Cuts at: 817 817
Size: 3440
BstBI TT'CG_AA
Cuts at: 1801 1801
Size: 3440
BstYI r'GATC_y
Cuts at: 1 465 932 955 1290 1536 2548 2634 2646
Size: 464 467 23 335 246 1012 86 12
Cuts at: 2646 2992 1
Size: 346 449
Fragments arranged by size:
1012 467 464 449 346 335 246 86
23 12
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 1390 1571 1734 1390
Size: 181 163 3096
Fragments arranged by size:
3096 181 163
BtsI GCAGTG_nn'
Cuts at: 683 683
Size: 3440
DraI TTT'AAA
Cuts at: 439 439
Size: 3440
DrdI GACnn_nn'nnGTC
Cuts at: 1146 2004 1146
Size: 858 2582
EaeI y'GGCC_r
Cuts at: 1025 1199 1590 1617 2962 1025
Size: 174 391 27 1345 1503
Fragments arranged by size:
1503 1345 391 174 27
EagI C'GGCC_G
Cuts at: 1025 1025
Size: 3440
EarI CTCTTCn'nnn_
Cuts at: 287 1463 1673 287
Size: 1176 210 2054
Fragments arranged by size:
2054 1176 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 391 1648 1958 2104 3250 391
Size: 1257 310 146 1146 581
Fragments arranged by size:
1257 1146 581 310 146
EcoICRI GAG'CTC
Cuts at: 452 452
Size: 3440
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 139 295 1265 1697 2444 2712 139
Size: 156 970 432 747 268 867
Fragments arranged by size:
970 867 747 432 268 156
EcoO109I rG'GnC_Cy
Cuts at: 579 579
Size: 3440
EcoRI G'AATT_C
Cuts at: 412 444 412
Size: 32 3408
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 313 1459 313
Size: 1146 2294
FspI TGC'GCA
Cuts at: 1221 1221
Size: 3440
HaeII r_GCGC'y
Cuts at: 154 874 1122 2144 3123 154
Size: 720 248 1022 979 471
Fragments arranged by size:
1022 979 720 471 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 264 296 1282 1312 1314 1344 264
Size: 32 986 30 2 30 2360
Fragments arranged by size:
2360 986 32 30 30 2
HincII GTy'rAC
Cuts at: 479 479
Size: 3440
KasI G'GCGC_C
Cuts at: 870 1118 870
Size: 248 3192
KpnI G_GTAC'C
Cuts at: 460 460
Size: 3440
MluI A'CGCG_T
Cuts at: 2873 2873
Size: 3440
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 2111 2295 2111
Size: 184 3256
MscI TGG'CCA
Cuts at: 1201 1201
Size: 3440
MslI CAynn'nnrTG
Cuts at: 121 1429 1556 121
Size: 1308 127 2005
Fragments arranged by size:
2005 1308 127
MspA1I CmG'CkG
Cuts at: 551 865 1225 2238 2483 551
Size: 314 360 1013 245 1508
Fragments arranged by size:
1508 1013 360 314 245
NaeI GCC'GGC
Cuts at: 1621 1621
Size: 3440
NarI GG'CG_CC
Cuts at: 871 1119 871
Size: 248 3192
NgoMIV G'CCGG_C
Cuts at: 1619 1619
Size: 3440
NspI r_CATG'y
Cuts at: 493 993 1900 493
Size: 500 907 2033
Fragments arranged by size:
2033 907 500
PciI A'CATG_T
Cuts at: 1896 1896
Size: 3440
PflMI CCAn_nnn'nTGG
Cuts at: 423 423
Size: 3440
PfoI T'CCnGG_A
Cuts at: 155 416 155
Size: 261 3179
PmeI GTTT'AAAC
Cuts at: 439 439
Size: 3440
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2579 2611 2579
Size: 32 3408
PstI C_TGCA'G
Cuts at: 487 1172 487
Size: 685 2755
PvuI CG_AT'CG
Cuts at: 104 104
Size: 3440
PvuII CAG'CTG
Cuts at: 865 1225 865
Size: 360 3080
RsrII CG'GwC_CG
Cuts at: 1635 1635
Size: 3440
SacI G_AGCT'C
Cuts at: 454 454
Size: 3440
SalI G'TCGA_C
Cuts at: 477 477
Size: 3440
SapI GCTCTTCn'nnn_
Cuts at: 287 1463 1673 287
Size: 1176 210 2054
Fragments arranged by size:
2054 1176 210
SbfI CC_TGCA'GG
Cuts at: 487 487
Size: 3440
ScaI AGT'ACT
Cuts at: 2957 2957
Size: 3440
SfcI C'TryA_G
Cuts at: 33 483 1168 2161 2352 33
Size: 450 685 993 191 1121
Fragments arranged by size:
1121 993 685 450 191
SfoI GGC'GCC
Cuts at: 872 1120 872
Size: 248 3192
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 3440
SmaI CCC'GGG
Cuts at: 462 462
Size: 3440
SmlI C'TyrA_G
Cuts at: 298 595 1510 2002 2264 298
Size: 297 915 492 262 1474
Fragments arranged by size:
1474 915 492 297 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 3440
SphI G_CATG'C
Cuts at: 493 993 493
Size: 500 2940
StuI AGG'CCT
Cuts at: 212 212
Size: 3440
StyI C'CwwG_G
Cuts at: 574 928 574
Size: 354 3086
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 358 1023 1156 1615 1824 358
Size: 665 133 459 209 1974
Fragments arranged by size:
1974 665 459 209 133
TatI w'GTAC_w
Cuts at: 2955 2955
Size: 3440
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2957 2989 2957
Size: 32 3408
Tth111I GACn'n_nGTC
Cuts at: 345 1237 345
Size: 892 2548
XbaI T'CTAG_A
Cuts at: 471 471
Size: 3440
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 363 935 363
Size: 572 2868
XmaI C'CCGG_G
Cuts at: 460 460
Size: 3440
ZraI GAC'GTC
Cuts at: 206 206
Size: 3440
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AfeI AflII AflIII
AgeI AleI AlwNI ApaLI ApoI AseI AsiSI AvaI
BamHI BanI BanII BbeI BciVI BclI BglII BlpI
Bme1580I BmgBI BmrI BplI BpmI BpuEI BsaI BsaAI
BsaBI BsaHI BsaWI BseYI BsiEI BsiHKAI BsmI Bsp1286I
BspEI BspHI BspMI BsrBI BsrDI BsrFI BssSI BstAPI
BstBI BstYI BtgZI BtsI DraI DrdI EaeI EagI
EarI EciI EcoICRI Eco57MI EcoO109I EcoRI FalI FspI
HaeII Hin4I HincII KasI KpnI MluI MmeI MscI
MslI MspA1I NaeI NarI NgoMIV NspI PciI PflMI
PfoI PmeI PpiI PstI PvuI PvuII RsrII SacI
SalI SapI SbfI ScaI SfcI SfoI SgfI SmaI
SmlI SpeI SphI StuI StyI TaqII TatI TstI
Tth111I XbaI XcmI XmaI ZraI
Enzymes that do not cut:
AarI AhdI AlfI AloI ApaI AscI AvrII BaeI
BbsI BbvCI BcgI BfrBI BglI BmtI Bpu10I BsaXI
BseRI BsgI BsiWI BsmBI BsrGI BssHII BstEII BstXI
BstZ17I Bsu36I BtgI ClaI CspCI DraIII EcoNI EcoRV
FseI FspAI HindIII HpaI MfeI NcoI NdeI NheI
NotI NruI NsiI PacI PasI PmlI PpuMI PshAI
PsiI PspOMI PspXI PsrI SacII SanDI SexAI SfiI
SgrAI SnaBI SrfI SspI SwaI XhoI XmnI