Restriction map for
pF1KE0264
(Circular) (Six-base) MAPSORT of: pF1KE0264.seq Check: 9491 from: 1 to: 4358
pF1KE0264 4358 bp
With 182 enzymes: SgfI *
April 24, 2009 13:12 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 822 822
Size: 4358
AccI GT'mk_AC
Cuts at: 1011 1396 1011
Size: 385 3973
Acc65I G'GTAC_C
Cuts at: 1374 1374
Size: 4358
AclI AA'CG_TT
Cuts at: 4198 4198
Size: 4358
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 2183 2615 3362 3630 2183
Size: 432 747 268 2911
Fragments arranged by size:
2911 747 432 268
AflII C'TTAA_G
Cuts at: 867 867
Size: 4358
AflIII A'CryG_T
Cuts at: 202 1710 2814 3791 202
Size: 1508 1104 977 769
Fragments arranged by size:
1508 1104 977 769
AgeI A'CCGG_T
Cuts at: 2746 2746
Size: 4358
AleI CACnn'nnGTG
Cuts at: 649 649
Size: 4358
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1294 1294
Size: 4358
AlwNI CAG_nnn'CTG
Cuts at: 1663 3230 1663
Size: 1567 2791
ApaLI G'TGCA_C
Cuts at: 3128 3128
Size: 4358
ApoI r'AATT_y
Cuts at: 1143 1362 3711 3967 1143
Size: 219 2349 256 1534
Fragments arranged by size:
2349 1534 256 219
AseI AT'TA_AT
Cuts at: 20 20
Size: 4358
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4358
AvaI C'yCGr_G
Cuts at: 346 1378 346
Size: 1032 3326
BamHI G'GATC_C
Cuts at: 1383 1383
Size: 4358
BanI G'GyrC_C
Cuts at: 129 1374 1788 2036 2071 129
Size: 1245 414 248 35 2416
Fragments arranged by size:
2416 1245 414 248 35
BanII G_rGCy'C
Cuts at: 450 1372 2402 450
Size: 922 1030 2406
Fragments arranged by size:
2406 1030 922
BbeI G_GCGC'C
Cuts at: 133 1792 2040 133
Size: 1659 248 2451
Fragments arranged by size:
2451 1659 248
BciVI GTATCCnnnnn_n'
Cuts at: 2256 3017 4358 2256
Size: 761 1341 2256
Fragments arranged by size:
2256 1341 761
BclI T'GATC_A
Cuts at: 1878 1878
Size: 4358
BglII A'GATC_T
Cuts at: 1873 3910 1873
Size: 2037 2321
BlpI GC'TnA_GC
Cuts at: 1470 1470
Size: 4358
Bme1580I G_kGCm'C
Cuts at: 1210 1983 2076 3132 1210
Size: 773 93 1056 2436
Fragments arranged by size:
2436 1056 773 93
BmrI ACTGGGnnnn_n'
Cuts at: 1570 1651 1986 4130 1570
Size: 81 335 2144 1798
Fragments arranged by size:
2144 1798 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2725 2725
Size: 4358
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 242 278 464 1048 242
Size: 36 186 584 3552
Fragments arranged by size:
3552 584 186 36
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1534 2413 2905 3203 1534
Size: 879 492 298 2689
Fragments arranged by size:
2689 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 183 4062 183
Size: 3879 479
BsaAI yAC'GTr
Cuts at: 1711 1711
Size: 4358
BsaBI GATnn'nnATC
Cuts at: 6 1872 3909 6
Size: 1866 2037 455
Fragments arranged by size:
2037 1866 455
BsaHI Gr'CG_yC
Cuts at: 130 1789 2037 2739 4235 130
Size: 1659 248 702 1496 253
Fragments arranged by size:
1659 1496 702 253 248
BsaWI w'CCGG_w
Cuts at: 1547 2068 2746 3020 3167 3578 1547
Size: 521 678 274 147 411 2327
Fragments arranged by size:
2327 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 437 467 437
Size: 30 4328
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1204 1204
Size: 4358
BseYI C'CCAG_C
Cuts at: 161 1783 2541 3118 3614 161
Size: 1622 758 577 496 905
Fragments arranged by size:
1622 905 758 577 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 499 800 499
Size: 301 4057
BsiEI CG_ry'CG
Cuts at: 104 1900 1946 3154 104
Size: 1796 46 1208 1308
Fragments arranged by size:
1796 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 1372 2150 2340 3132 1372
Size: 778 190 792 2598
Fragments arranged by size:
2598 792 778 190
BsmI GAATG_Cn'
Cuts at: 212 212
Size: 4358
Bsp1286I G_dGCh'C
Cuts at: 450 1210 1372 1983 2076 2150 2340 2402 3132
Size: 760 162 611 93 74 190 62 730
Cuts at: 3132 450
Size: 1676
Fragments arranged by size:
1676 760 730 611 190 162 93 74
62
BspEI T'CCGG_A
Cuts at: 3578 3578
Size: 4358
BspHI T'CATG_A
Cuts at: 1295 3534 1295
Size: 2239 2119
BspMI ACCTGCnnnn'nnnn_
Cuts at: 822 1408 1622 1924 2305 822
Size: 586 214 302 381 2875
Fragments arranged by size:
2875 586 381 302 214
BsrBI CCG'CTC
Cuts at: 50 2650 2704 4188 4353 50
Size: 2600 54 1484 165 55
Fragments arranged by size:
2600 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 516 1354 2270 516
Size: 838 916 2604
Fragments arranged by size:
2604 916 838
BsrFI r'CCGG_y
Cuts at: 1063 2356 2537 2746 1063
Size: 1293 181 209 2675
Fragments arranged by size:
2675 1293 209 181
BsrGI T'GTAC_A
Cuts at: 386 386
Size: 4358
BssSI C'ACGA_G
Cuts at: 2629 2987 2629
Size: 358 4000
BstAPI GCAn_nnn'nTGC
Cuts at: 1205 1735 1205
Size: 530 3828
BstBI TT'CG_AA
Cuts at: 2719 2719
Size: 4358
BstXI CCAn_nnnn'nTGG
Cuts at: 403 415 403
Size: 12 4346
BstYI r'GATC_y
Cuts at: 1 1383 1850 1873 2208 2454 3466 3552 3564
Size: 1382 467 23 335 246 1012 86 12
Cuts at: 3564 3910 1
Size: 346 449
Fragments arranged by size:
1382 1012 467 449 346 335 246 86
23 12
BtgI C'CryG_G
Cuts at: 968 986 968
Size: 18 4340
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 1106 2308 2489 2652 1106
Size: 1202 181 163 2812
Fragments arranged by size:
2812 1202 181 163
BtsI GCAGTG_nn'
Cuts at: 249 1210 1601 249
Size: 961 391 3006
Fragments arranged by size:
3006 961 391
DraI TTT'AAA
Cuts at: 603 977 1357 603
Size: 374 380 3604
Fragments arranged by size:
3604 380 374
DrdI GACnn_nn'nnGTC
Cuts at: 2064 2922 2064
Size: 858 3500
EaeI y'GGCC_r
Cuts at: 393 405 417 521 616 1943 2117 2508 2535
Size: 12 12 104 95 1327 174 391 27
Cuts at: 2535 3880 393
Size: 1345 871
Fragments arranged by size:
1345 1327 871 391 174 104 95 27
12 12
EagI C'GGCC_G
Cuts at: 1943 1943
Size: 4358
EarI CTCTTCn'nnn_
Cuts at: 1215 2381 2591 1215
Size: 1166 210 2982
Fragments arranged by size:
2982 1166 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2566 2876 3022 4168 2566
Size: 310 146 1146 2756
Fragments arranged by size:
2756 1146 310 146
EcoICRI GAG'CTC
Cuts at: 1370 1370
Size: 4358
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 242 278 464 1048 2183 2615 3362 3630 242
Size: 36 186 584 1135 432 747 268 970
Fragments arranged by size:
1135 970 747 584 432 268 186 36
EcoO109I rG'GnC_Cy
Cuts at: 1497 1497
Size: 4358
EcoRI G'AATT_C
Cuts at: 1362 1362
Size: 4358
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2377 2377
Size: 4358
FspI TGC'GCA
Cuts at: 2139 2139
Size: 4358
HaeII r_GCGC'y
Cuts at: 133 1792 2040 3062 4041 133
Size: 1659 248 1022 979 450
Fragments arranged by size:
1659 1022 979 450 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1008 1040 1259 1291 1299 1331 2200 2230 2232
Size: 32 219 32 8 32 869 30 2
Cuts at: 2232 2262 1008
Size: 30 3104
Fragments arranged by size:
3104 869 219 32 32 32 30 30
8 2
HincII GTy'rAC
Cuts at: 771 1000 1397 771
Size: 229 397 3732
Fragments arranged by size:
3732 397 229
KasI G'GCGC_C
Cuts at: 129 1788 2036 129
Size: 1659 248 2451
Fragments arranged by size:
2451 1659 248
KpnI G_GTAC'C
Cuts at: 1378 1378
Size: 4358
MfeI C'AATT_G
Cuts at: 821 821
Size: 4358
MluI A'CGCG_T
Cuts at: 3791 3791
Size: 4358
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 3029 3213 3029
Size: 184 4174
MscI TGG'CCA
Cuts at: 395 407 523 618 2119 395
Size: 12 116 95 1501 2634
Fragments arranged by size:
2634 1501 116 95 12
MslI CAynn'nnrTG
Cuts at: 401 523 649 1240 2347 2474 401
Size: 122 126 591 1107 127 2285
Fragments arranged by size:
2285 1107 591 127 126 122
MspA1I CmG'CkG
Cuts at: 505 535 1469 1783 2143 3156 3401 505
Size: 30 934 314 360 1013 245 1462
Fragments arranged by size:
1462 1013 934 360 314 245 30
NaeI GCC'GGC
Cuts at: 1065 2539 1065
Size: 1474 2884
NarI GG'CG_CC
Cuts at: 130 1789 2037 130
Size: 1659 248 2451
Fragments arranged by size:
2451 1659 248
NgoMIV G'CCGG_C
Cuts at: 1063 2537 1063
Size: 1474 2884
NruI TCG'CGA
Cuts at: 1091 1091
Size: 4358
NspI r_CATG'y
Cuts at: 206 1411 1911 2818 206
Size: 1205 500 907 1746
Fragments arranged by size:
1746 1205 907 500
PciI A'CATG_T
Cuts at: 202 2814 202
Size: 2612 1746
PfoI T'CCnGG_A
Cuts at: 255 1325 255
Size: 1070 3288
PmeI GTTT'AAAC
Cuts at: 1357 1357
Size: 4358
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3497 3529 3497
Size: 32 4326
PshAI GACnn'nnGTC
Cuts at: 1192 1192
Size: 4358
PstI C_TGCA'G
Cuts at: 639 882 1405 2090 639
Size: 243 523 685 2907
Fragments arranged by size:
2907 685 523 243
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4358
PvuII CAG'CTG
Cuts at: 505 1783 2143 505
Size: 1278 360 2720
Fragments arranged by size:
2720 1278 360
RsrII CG'GwC_CG
Cuts at: 2553 2553
Size: 4358
SacI G_AGCT'C
Cuts at: 1372 1372
Size: 4358
SalI G'TCGA_C
Cuts at: 1395 1395
Size: 4358
SapI GCTCTTCn'nnn_
Cuts at: 2381 2591 2381
Size: 210 4148
SbfI CC_TGCA'GG
Cuts at: 639 1405 639
Size: 766 3592
ScaI AGT'ACT
Cuts at: 3875 3875
Size: 4358
SfcI C'TryA_G
Cuts at: 33 635 878 932 1401 2086 3079 3270 33
Size: 602 243 54 469 685 993 191 1121
Fragments arranged by size:
1121 993 685 602 469 243 191 54
SfoI GGC'GCC
Cuts at: 131 1790 2038 131
Size: 1659 248 2451
Fragments arranged by size:
2451 1659 248
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4358
SmaI CCC'GGG
Cuts at: 1380 1380
Size: 4358
SmlI C'TyrA_G
Cuts at: 346 867 1513 2428 2920 3182 346
Size: 521 646 915 492 262 1522
Fragments arranged by size:
1522 915 646 521 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4358
SphI G_CATG'C
Cuts at: 1411 1911 1411
Size: 500 3858
StyI C'CwwG_G
Cuts at: 1492 1846 1492
Size: 354 4004
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 175 1941 2074 2533 2742 175
Size: 1766 133 459 209 1791
Fragments arranged by size:
1791 1766 459 209 133
TatI w'GTAC_w
Cuts at: 283 386 3873 283
Size: 103 3487 768
Fragments arranged by size:
3487 768 103
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 437 469 3875 3907 437
Size: 32 3406 32 888
Fragments arranged by size:
3406 888 32 32
Tth111I GACn'n_nGTC
Cuts at: 2155 2155
Size: 4358
XbaI T'CTAG_A
Cuts at: 1389 1389
Size: 4358
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1853 1853
Size: 4358
XhoI C'TCGA_G
Cuts at: 346 346
Size: 4358
XmaI C'CCGG_G
Cuts at: 1378 1378
Size: 4358
XmnI GAAnn'nnTTC
Cuts at: 251 251
Size: 4358
Enzymes that do cut:
AarI AccI Acc65I AclI AcuI AflII AflIII AgeI
AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI
BamHI BanI BanII BbeI BciVI BclI BglII BlpI
Bme1580I BmrI BplI BpmI BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI
BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BsrGI BssSI BstAPI BstBI BstXI BstYI BtgI BtgZI
BtsI DraI DrdI EaeI EagI EarI EciI EcoICRI
Eco57MI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII
KasI KpnI MfeI MluI MmeI MscI MslI MspA1I
NaeI NarI NgoMIV NruI NspI PciI PfoI PmeI
PpiI PshAI PstI PvuI PvuII RsrII SacI SalI
SapI SbfI ScaI SfcI SfoI SgfI SmaI SmlI
SpeI SphI StyI TaqII TatI TstI Tth111I XbaI
XcmI XhoI XmaI XmnI
Enzymes that do not cut:
AatII AfeI AhdI AloI ApaI AscI AvrII BaeI
BbsI BbvCI BcgI BfrBI BglI BmgBI BmtI Bpu10I
BsiWI BsmBI BssHII BstEII BstZ17I Bsu36I ClaI CspCI
DraIII EcoNI EcoRV FseI FspAI HindIII HpaI NcoI
NdeI NheI NotI NsiI PacI PasI PflMI PmlI
PpuMI PsiI PspOMI PspXI PsrI SacII SanDI SexAI
SfiI SgrAI SnaBI SrfI SspI StuI SwaI ZraI