Restriction map for
pF1KE0260
(Circular) (Six-base) MAPSORT of: pF1KE0260.seq Check: 9705 from: 1 to: 3407
pF1KE0260 3407 bp
With 182 enzymes: SgfI *
April 24, 2009 13:12 ..
AccI GT'mk_AC
Cuts at: 445 445
Size: 3407
Acc65I G'GTAC_C
Cuts at: 423 423
Size: 3407
AclI AA'CG_TT
Cuts at: 3247 3247
Size: 3407
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 309 1232 1664 2411 2679 309
Size: 923 432 747 268 1037
Fragments arranged by size:
1037 923 747 432 268
AflIII A'CryG_T
Cuts at: 759 1863 2840 759
Size: 1104 977 1326
Fragments arranged by size:
1326 1104 977
AgeI A'CCGG_T
Cuts at: 1795 1795
Size: 3407
AlwNI CAG_nnn'CTG
Cuts at: 712 2279 712
Size: 1567 1840
ApaLI G'TGCA_C
Cuts at: 2177 2177
Size: 3407
ApoI r'AATT_y
Cuts at: 411 2760 3016 411
Size: 2349 256 802
Fragments arranged by size:
2349 802 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 3407
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 3407
AvaI C'yCGr_G
Cuts at: 427 427
Size: 3407
BamHI G'GATC_C
Cuts at: 432 432
Size: 3407
BanI G'GyrC_C
Cuts at: 423 837 1085 1120 423
Size: 414 248 35 2710
Fragments arranged by size:
2710 414 248 35
BanII G_rGCy'C
Cuts at: 421 1451 421
Size: 1030 2377
BbeI G_GCGC'C
Cuts at: 841 1089 841
Size: 248 3159
BciVI GTATCCnnnnn_n'
Cuts at: 1305 2066 3407 1305
Size: 761 1341 1305
Fragments arranged by size:
1341 1305 761
BclI T'GATC_A
Cuts at: 927 927
Size: 3407
BglII A'GATC_T
Cuts at: 371 922 2959 371
Size: 551 2037 819
Fragments arranged by size:
2037 819 551
BlpI GC'TnA_GC
Cuts at: 519 519
Size: 3407
Bme1580I G_kGCm'C
Cuts at: 1032 1125 2181 1032
Size: 93 1056 2258
Fragments arranged by size:
2258 1056 93
BmrI ACTGGGnnnn_n'
Cuts at: 619 700 1035 3179 619
Size: 81 335 2144 847
Fragments arranged by size:
2144 847 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 1774 1774
Size: 3407
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 583 1462 1954 2252 583
Size: 879 492 298 1738
Fragments arranged by size:
1738 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 3111 3111
Size: 3407
BsaAI yAC'GTr
Cuts at: 760 760
Size: 3407
BsaBI GATnn'nnATC
Cuts at: 6 921 2958 6
Size: 915 2037 455
Fragments arranged by size:
2037 915 455
BsaHI Gr'CG_yC
Cuts at: 838 1086 1788 3284 838
Size: 248 702 1496 961
Fragments arranged by size:
1496 961 702 248
BsaWI w'CCGG_w
Cuts at: 231 596 1117 1795 2069 2216 2627 231
Size: 365 521 678 274 147 411 1011
Fragments arranged by size:
1011 678 521 411 365 274 147
BseYI C'CCAG_C
Cuts at: 832 1590 2167 2663 832
Size: 758 577 496 1576
Fragments arranged by size:
1576 758 577 496
BsiEI CG_ry'CG
Cuts at: 104 949 995 2203 104
Size: 845 46 1208 1308
Fragments arranged by size:
1308 1208 845 46
BsiHKAI G_wGCw'C
Cuts at: 421 1199 1389 2181 421
Size: 778 190 792 1647
Fragments arranged by size:
1647 792 778 190
Bsp1286I G_dGCh'C
Cuts at: 421 1032 1125 1199 1389 1451 2181 421
Size: 611 93 74 190 62 730 1647
Fragments arranged by size:
1647 730 611 190 93 74 62
BspEI T'CCGG_A
Cuts at: 231 2627 231
Size: 2396 1011
BspHI T'CATG_A
Cuts at: 2583 2583
Size: 3407
BspMI ACCTGCnnnn'nnnn_
Cuts at: 457 671 973 1354 457
Size: 214 302 381 2510
Fragments arranged by size:
2510 381 302 214
BsrBI CCG'CTC
Cuts at: 50 1699 1753 3237 3402 50
Size: 1649 54 1484 165 55
Fragments arranged by size:
1649 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 1319 1319
Size: 3407
BsrFI r'CCGG_y
Cuts at: 1405 1586 1795 1405
Size: 181 209 3017
Fragments arranged by size:
3017 209 181
BssSI C'ACGA_G
Cuts at: 1678 2036 1678
Size: 358 3049
BstAPI GCAn_nnn'nTGC
Cuts at: 784 784
Size: 3407
BstBI TT'CG_AA
Cuts at: 1768 1768
Size: 3407
BstYI r'GATC_y
Cuts at: 1 371 432 899 922 1257 1503 2515 2601
Size: 370 61 467 23 335 246 1012 86
Cuts at: 2601 2613 2959 1
Size: 12 346 449
Fragments arranged by size:
1012 467 449 370 346 335 246 86
61 23 12
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 1357 1538 1701 1357
Size: 181 163 3063
Fragments arranged by size:
3063 181 163
BtsI GCAGTG_nn'
Cuts at: 650 650
Size: 3407
DraI TTT'AAA
Cuts at: 406 406
Size: 3407
DrdI GACnn_nn'nnGTC
Cuts at: 1113 1971 1113
Size: 858 2549
EaeI y'GGCC_r
Cuts at: 992 1166 1557 1584 2929 992
Size: 174 391 27 1345 1470
Fragments arranged by size:
1470 1345 391 174 27
EagI C'GGCC_G
Cuts at: 992 992
Size: 3407
EarI CTCTTCn'nnn_
Cuts at: 1430 1640 1430
Size: 210 3197
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 1615 1925 2071 3217 1615
Size: 310 146 1146 1805
Fragments arranged by size:
1805 1146 310 146
EcoICRI GAG'CTC
Cuts at: 419 419
Size: 3407
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 309 1232 1664 2411 2679 309
Size: 923 432 747 268 1037
Fragments arranged by size:
1037 923 747 432 268
EcoO109I rG'GnC_Cy
Cuts at: 546 546
Size: 3407
EcoRI G'AATT_C
Cuts at: 411 411
Size: 3407
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 1426 1426
Size: 3407
FspI TGC'GCA
Cuts at: 1188 1188
Size: 3407
HaeII r_GCGC'y
Cuts at: 841 1089 2111 3090 841
Size: 248 1022 979 1158
Fragments arranged by size:
1158 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 356 388 1249 1279 1281 1311 356
Size: 32 861 30 2 30 2452
Fragments arranged by size:
2452 861 32 30 30 2
HincII GTy'rAC
Cuts at: 446 446
Size: 3407
KasI G'GCGC_C
Cuts at: 837 1085 837
Size: 248 3159
KpnI G_GTAC'C
Cuts at: 427 427
Size: 3407
MluI A'CGCG_T
Cuts at: 2840 2840
Size: 3407
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 2078 2262 2078
Size: 184 3223
MscI TGG'CCA
Cuts at: 1168 1168
Size: 3407
MslI CAynn'nnrTG
Cuts at: 1396 1523 1396
Size: 127 3280
MspA1I CmG'CkG
Cuts at: 518 832 1192 2205 2450 518
Size: 314 360 1013 245 1475
Fragments arranged by size:
1475 1013 360 314 245
NaeI GCC'GGC
Cuts at: 1588 1588
Size: 3407
NarI GG'CG_CC
Cuts at: 838 1086 838
Size: 248 3159
NgoMIV G'CCGG_C
Cuts at: 1586 1586
Size: 3407
NspI r_CATG'y
Cuts at: 460 960 1867 460
Size: 500 907 2000
Fragments arranged by size:
2000 907 500
PciI A'CATG_T
Cuts at: 1863 1863
Size: 3407
PfoI T'CCnGG_A
Cuts at: 272 272
Size: 3407
PmeI GTTT'AAAC
Cuts at: 406 406
Size: 3407
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2546 2578 2546
Size: 32 3375
PstI C_TGCA'G
Cuts at: 454 1139 454
Size: 685 2722
PvuI CG_AT'CG
Cuts at: 104 104
Size: 3407
PvuII CAG'CTG
Cuts at: 832 1192 832
Size: 360 3047
RsrII CG'GwC_CG
Cuts at: 1602 1602
Size: 3407
SacI G_AGCT'C
Cuts at: 421 421
Size: 3407
SalI G'TCGA_C
Cuts at: 444 444
Size: 3407
SapI GCTCTTCn'nnn_
Cuts at: 1430 1640 1430
Size: 210 3197
SbfI CC_TGCA'GG
Cuts at: 454 454
Size: 3407
ScaI AGT'ACT
Cuts at: 2924 2924
Size: 3407
SfcI C'TryA_G
Cuts at: 33 192 450 1135 2128 2319 33
Size: 159 258 685 993 191 1121
Fragments arranged by size:
1121 993 685 258 191 159
SfoI GGC'GCC
Cuts at: 839 1087 839
Size: 248 3159
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 3407
SmaI CCC'GGG
Cuts at: 429 429
Size: 3407
SmlI C'TyrA_G
Cuts at: 562 1477 1969 2231 562
Size: 915 492 262 1738
Fragments arranged by size:
1738 915 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 3407
SphI G_CATG'C
Cuts at: 460 960 460
Size: 500 2907
StyI C'CwwG_G
Cuts at: 541 895 541
Size: 354 3053
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 990 1123 1582 1791 990
Size: 133 459 209 2606
Fragments arranged by size:
2606 459 209 133
TatI w'GTAC_w
Cuts at: 2922 2922
Size: 3407
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 285 317 2924 2956 285
Size: 32 2607 32 736
Fragments arranged by size:
2607 736 32 32
Tth111I GACn'n_nGTC
Cuts at: 1204 1204
Size: 3407
XbaI T'CTAG_A
Cuts at: 438 438
Size: 3407
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 114 902 114
Size: 788 2619
XmaI C'CCGG_G
Cuts at: 427 427
Size: 3407
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AgeI AlwNI ApaLI
ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI
BciVI BclI BglII BlpI Bme1580I BmrI BplI BpuEI
BsaI BsaAI BsaBI BsaHI BsaWI BseYI BsiEI BsiHKAI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BssSI
BstAPI BstBI BstYI BtgZI BtsI DraI DrdI EaeI
EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI FalI
FspI HaeII Hin4I HincII KasI KpnI MluI MmeI
MscI MslI MspA1I NaeI NarI NgoMIV NspI PciI
PfoI PmeI PpiI PstI PvuI PvuII RsrII SacI
SalI SapI SbfI ScaI SfcI SfoI SgfI SmaI
SmlI SpeI SphI StyI TaqII TatI TstI Tth111I
XbaI XcmI XmaI
Enzymes that do not cut:
AarI AatII AfeI AflII AhdI AleI AlfI AloI
ApaI AscI AvrII BaeI BbsI BbvCI BcgI BfrBI
BglI BmgBI BmtI BpmI Bpu10I BsaXI BseRI BsgI
BsiWI BsmI BsmBI BsrGI BssHII BstEII BstXI BstZ17I
Bsu36I BtgI ClaI CspCI DraIII EcoNI EcoRV FseI
FspAI HindIII HpaI MfeI NcoI NdeI NheI NotI
NruI NsiI PacI PasI PflMI PmlI PpuMI PshAI
PsiI PspOMI PspXI PsrI SacII SanDI SexAI SfiI
SgrAI SnaBI SrfI SspI StuI SwaI XhoI XmnI
ZraI