Restriction map for
pF1KE0259
(Circular) (Six-base) MAPSORT of: pF1KE0259.seq Check: 505 from: 1 to: 3863
pF1KE0259 3863 bp
With 182 enzymes: SgfI *
April 24, 2009 13:12 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 108 270 108
Size: 162 3701
AccI GT'mk_AC
Cuts at: 901 901
Size: 3863
Acc65I G'GTAC_C
Cuts at: 879 879
Size: 3863
AclI AA'CG_TT
Cuts at: 3703 3703
Size: 3863
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 133 556 603 1688 2120 2867 3135 133
Size: 423 47 1085 432 747 268 861
Fragments arranged by size:
1085 861 747 432 423 268 47
AflIII A'CryG_T
Cuts at: 1215 2319 3296 1215
Size: 1104 977 1782
Fragments arranged by size:
1782 1104 977
AgeI A'CCGG_T
Cuts at: 2251 2251
Size: 3863
AleI CACnn'nnGTG
Cuts at: 256 710 256
Size: 454 3409
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 319 319
Size: 3863
AlwNI CAG_nnn'CTG
Cuts at: 651 1168 2735 651
Size: 517 1567 1779
Fragments arranged by size:
1779 1567 517
ApaLI G'TGCA_C
Cuts at: 376 2633 376
Size: 2257 1606
ApoI r'AATT_y
Cuts at: 867 3216 3472 867
Size: 2349 256 1258
Fragments arranged by size:
2349 1258 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 3863
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 3863
AvaI C'yCGr_G
Cuts at: 761 883 761
Size: 122 3741
BamHI G'GATC_C
Cuts at: 888 888
Size: 3863
BanI G'GyrC_C
Cuts at: 879 1293 1541 1576 879
Size: 414 248 35 3166
Fragments arranged by size:
3166 414 248 35
BanII G_rGCy'C
Cuts at: 365 425 454 515 566 877 1907 365
Size: 60 29 61 51 311 1030 2321
Fragments arranged by size:
2321 1030 311 61 60 51 29
BbeI G_GCGC'C
Cuts at: 1297 1545 1297
Size: 248 3615
BbsI GAAGACnn'nnnn_
Cuts at: 165 609 165
Size: 444 3419
BciVI GTATCCnnnnn_n'
Cuts at: 1761 2522 3863 1761
Size: 761 1341 1761
Fragments arranged by size:
1761 1341 761
BclI T'GATC_A
Cuts at: 1383 1383
Size: 3863
BglII A'GATC_T
Cuts at: 1378 3415 1378
Size: 2037 1826
BlpI GC'TnA_GC
Cuts at: 394 975 394
Size: 581 3282
Bme1580I G_kGCm'C
Cuts at: 380 1488 1581 2637 380
Size: 1108 93 1056 1606
Fragments arranged by size:
1606 1108 1056 93
BmrI ACTGGGnnnn_n'
Cuts at: 1075 1156 1491 3635 1075
Size: 81 335 2144 1303
Fragments arranged by size:
2144 1303 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2230 2230
Size: 3863
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 151 157 187 709 763 837 151
Size: 6 30 522 54 74 3177
Fragments arranged by size:
3177 522 74 54 30 6
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1039 1918 2410 2708 1039
Size: 879 492 298 2194
Fragments arranged by size:
2194 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 3567 3567
Size: 3863
BsaAI yAC'GTr
Cuts at: 1216 1216
Size: 3863
BsaBI GATnn'nnATC
Cuts at: 6 1377 3414 6
Size: 1371 2037 455
Fragments arranged by size:
2037 1371 455
BsaHI Gr'CG_yC
Cuts at: 1294 1542 2244 3740 1294
Size: 248 702 1496 1417
Fragments arranged by size:
1496 1417 702 248
BsaWI w'CCGG_w
Cuts at: 383 587 1052 1573 2251 2525 2672 3083 383
Size: 204 465 521 678 274 147 411 1163
Fragments arranged by size:
1163 678 521 465 411 274 204 147
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 523 685 523
Size: 162 3701
BseYI C'CCAG_C
Cuts at: 447 623 1288 2046 2623 3119 447
Size: 176 665 758 577 496 1191
Fragments arranged by size:
1191 758 665 577 496 176
BsiEI CG_ry'CG
Cuts at: 104 1405 1451 2659 104
Size: 1301 46 1208 1308
Fragments arranged by size:
1308 1301 1208 46
BsiHKAI G_wGCw'C
Cuts at: 380 425 515 566 877 1655 1845 2637 380
Size: 45 90 51 311 778 190 792 1606
Fragments arranged by size:
1606 792 778 311 190 90 51 45
BsmBI CGTCTCn'nnnn_
Cuts at: 318 486 318
Size: 168 3695
Bsp1286I G_dGCh'C
Cuts at: 365 380 425 454 515 566 877 1488 1581
Size: 15 45 29 61 51 311 611 93
Cuts at: 1581 1655 1845 1907 2637 365
Size: 74 190 62 730 1591
Fragments arranged by size:
1591 730 611 311 190 93 74 62
61 51 45 29 15
BspEI T'CCGG_A
Cuts at: 3083 3083
Size: 3863
BspHI T'CATG_A
Cuts at: 3039 3039
Size: 3863
BspMI ACCTGCnnnn'nnnn_
Cuts at: 108 270 913 1127 1429 1810 108
Size: 162 643 214 302 381 2161
Fragments arranged by size:
2161 643 381 302 214 162
BsrBI CCG'CTC
Cuts at: 50 393 768 2155 2209 3693 3858 50
Size: 343 375 1387 54 1484 165 55
Fragments arranged by size:
1484 1387 375 343 165 55 54
BsrDI GCAATG_nn'
Cuts at: 813 1775 813
Size: 962 2901
BsrFI r'CCGG_y
Cuts at: 143 1861 2042 2251 143
Size: 1718 181 209 1755
Fragments arranged by size:
1755 1718 209 181
BssSI C'ACGA_G
Cuts at: 2134 2492 2134
Size: 358 3505
BstAPI GCAn_nnn'nTGC
Cuts at: 221 1240 221
Size: 1019 2844
BstBI TT'CG_AA
Cuts at: 2224 2224
Size: 3863
BstXI CCAn_nnnn'nTGG
Cuts at: 165 165
Size: 3863
BstYI r'GATC_y
Cuts at: 1 888 1355 1378 1713 1959 2971 3057 3069
Size: 887 467 23 335 246 1012 86 12
Cuts at: 3069 3415 1
Size: 346 449
Fragments arranged by size:
1012 887 467 449 346 335 246 86
23 12
Bsu36I CC'TnA_GG
Cuts at: 340 340
Size: 3863
BtgI C'CryG_G
Cuts at: 107 711 845 107
Size: 604 134 3125
Fragments arranged by size:
3125 604 134
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 552 1813 1994 2157 552
Size: 1261 181 163 2258
Fragments arranged by size:
2258 1261 181 163
BtsI GCAGTG_nn'
Cuts at: 1106 1106
Size: 3863
DraI TTT'AAA
Cuts at: 862 862
Size: 3863
DraIII CAC_nnn'GTG
Cuts at: 663 663
Size: 3863
DrdI GACnn_nn'nnGTC
Cuts at: 1569 2427 1569
Size: 858 3005
EaeI y'GGCC_r
Cuts at: 201 1448 1622 2013 2040 3385 201
Size: 1247 174 391 27 1345 679
Fragments arranged by size:
1345 1247 679 391 174 27
EagI C'GGCC_G
Cuts at: 1448 1448
Size: 3863
EarI CTCTTCn'nnn_
Cuts at: 529 1886 2096 529
Size: 1357 210 2296
Fragments arranged by size:
2296 1357 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 622 2071 2381 2527 3673 622
Size: 1449 310 146 1146 812
Fragments arranged by size:
1449 1146 812 310 146
EcoICRI GAG'CTC
Cuts at: 423 513 564 875 423
Size: 90 51 311 3411
Fragments arranged by size:
3411 311 90 51
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 133 151 157 187 556 603 709 763 837
Size: 18 6 30 369 47 106 54 74
Cuts at: 837 1688 2120 2867 3135 133
Size: 851 432 747 268 861
Fragments arranged by size:
861 851 747 432 369 268 106 74
54 47 30 18 6
EcoO109I rG'GnC_Cy
Cuts at: 354 1002 354
Size: 648 3215
EcoRI G'AATT_C
Cuts at: 867 867
Size: 3863
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 1882 1882
Size: 3863
FspI TGC'GCA
Cuts at: 1644 1644
Size: 3863
HaeII r_GCGC'y
Cuts at: 1297 1545 2567 3546 1297
Size: 248 1022 979 1614
Fragments arranged by size:
1614 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 687 719 1705 1735 1737 1767 687
Size: 32 986 30 2 30 2783
Fragments arranged by size:
2783 986 32 30 30 2
HincII GTy'rAC
Cuts at: 902 902
Size: 3863
KasI G'GCGC_C
Cuts at: 1293 1541 1293
Size: 248 3615
KpnI G_GTAC'C
Cuts at: 883 883
Size: 3863
MluI A'CGCG_T
Cuts at: 3296 3296
Size: 3863
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 590 2534 2718 590
Size: 1944 184 1735
Fragments arranged by size:
1944 1735 184
MscI TGG'CCA
Cuts at: 1624 1624
Size: 3863
MslI CAynn'nnrTG
Cuts at: 256 710 1852 1979 256
Size: 454 1142 127 2140
Fragments arranged by size:
2140 1142 454 127
MspA1I CmG'CkG
Cuts at: 623 683 741 974 1288 1648 2661 2906 623
Size: 60 58 233 314 360 1013 245 1580
Fragments arranged by size:
1580 1013 360 314 245 233 60 58
NaeI GCC'GGC
Cuts at: 2044 2044
Size: 3863
NarI GG'CG_CC
Cuts at: 1294 1542 1294
Size: 248 3615
NcoI C'CATG_G
Cuts at: 107 845 107
Size: 738 3125
NgoMIV G'CCGG_C
Cuts at: 2042 2042
Size: 3863
NspI r_CATG'y
Cuts at: 738 916 1416 2323 738
Size: 178 500 907 2278
Fragments arranged by size:
2278 907 500 178
PasI CC'CwG_GG
Cuts at: 349 753 349
Size: 404 3459
PciI A'CATG_T
Cuts at: 2319 2319
Size: 3863
PflMI CCAn_nnn'nTGG
Cuts at: 113 275 663 846 113
Size: 162 388 183 3130
Fragments arranged by size:
3130 388 183 162
PfoI T'CCnGG_A
Cuts at: 644 644
Size: 3863
PmeI GTTT'AAAC
Cuts at: 862 862
Size: 3863
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3002 3034 3002
Size: 32 3831
PpuMI rG'GwC_Cy
Cuts at: 354 354
Size: 3863
PstI C_TGCA'G
Cuts at: 496 910 1595 496
Size: 414 685 2764
Fragments arranged by size:
2764 685 414
PvuI CG_AT'CG
Cuts at: 104 104
Size: 3863
PvuII CAG'CTG
Cuts at: 623 741 1288 1648 623
Size: 118 547 360 2838
Fragments arranged by size:
2838 547 360 118
RsrII CG'GwC_CG
Cuts at: 2058 2058
Size: 3863
SacI G_AGCT'C
Cuts at: 425 515 566 877 425
Size: 90 51 311 3411
Fragments arranged by size:
3411 311 90 51
SalI G'TCGA_C
Cuts at: 900 900
Size: 3863
SanDI GG'GwC_CC
Cuts at: 354 354
Size: 3863
SapI GCTCTTCn'nnn_
Cuts at: 1886 2096 1886
Size: 210 3653
SbfI CC_TGCA'GG
Cuts at: 910 910
Size: 3863
ScaI AGT'ACT
Cuts at: 3380 3380
Size: 3863
SfcI C'TryA_G
Cuts at: 33 193 492 906 1591 2584 2775 33
Size: 160 299 414 685 993 191 1121
Fragments arranged by size:
1121 993 685 414 299 191 160
SfoI GGC'GCC
Cuts at: 1295 1543 1295
Size: 248 3615
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 3863
SmaI CCC'GGG
Cuts at: 763 885 763
Size: 122 3741
SmlI C'TyrA_G
Cuts at: 1018 1933 2425 2687 1018
Size: 915 492 262 2194
Fragments arranged by size:
2194 915 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 3863
SphI G_CATG'C
Cuts at: 916 1416 916
Size: 500 3363
StyI C'CwwG_G
Cuts at: 107 254 365 845 997 1351 107
Size: 147 111 480 152 354 2619
Fragments arranged by size:
2619 480 354 152 147 111
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 315 615 1446 1579 2038 2247 315
Size: 300 831 133 459 209 1931
Fragments arranged by size:
1931 831 459 300 209 133
TatI w'GTAC_w
Cuts at: 3378 3378
Size: 3863
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3380 3412 3380
Size: 32 3831
Tth111I GACn'n_nGTC
Cuts at: 1660 1660
Size: 3863
XbaI T'CTAG_A
Cuts at: 894 894
Size: 3863
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 315 1358 315
Size: 1043 2820
XmaI C'CCGG_G
Cuts at: 761 883 761
Size: 122 3741
Enzymes that do cut:
AarI AccI Acc65I AclI AcuI AflIII AgeI AleI
AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI
BanI BanII BbeI BbsI BciVI BclI BglII BlpI
Bme1580I BmrI BplI BpmI BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BseRI BseYI BsiEI BsiHKAI BsmBI Bsp1286I
BspEI BspHI BspMI BsrBI BsrDI BsrFI BssSI BstAPI
BstBI BstXI BstYI Bsu36I BtgI BtgZI BtsI DraI
DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI
EcoO109I EcoRI FalI FspI HaeII Hin4I HincII KasI
KpnI MluI MmeI MscI MslI MspA1I NaeI NarI
NcoI NgoMIV NspI PasI PciI PflMI PfoI PmeI
PpiI PpuMI PstI PvuI PvuII RsrII SacI SalI
SanDI SapI SbfI ScaI SfcI SfoI SgfI SmaI
SmlI SpeI SphI StyI TaqII TatI TstI Tth111I
XbaI XcmI XmaI
Enzymes that do not cut:
AatII AfeI AflII AhdI AloI ApaI AscI AvrII
BaeI BbvCI BcgI BfrBI BglI BmgBI BmtI Bpu10I
BsaXI BsgI BsiWI BsmI BsrGI BssHII BstEII BstZ17I
ClaI CspCI EcoNI EcoRV FseI FspAI HindIII HpaI
MfeI NdeI NheI NotI NruI NsiI PacI PmlI
PshAI PsiI PspOMI PspXI PsrI SacII SexAI SfiI
SgrAI SnaBI SrfI SspI StuI SwaI XhoI XmnI
ZraI