Restriction map for
pF1KE0256
(Circular) (Six-base) MAPSORT of: pF1KE0256.seq Check: 9161 from: 1 to: 4310
pF1KE0256 4310 bp
With 182 enzymes: SgfI *
April 24, 2009 13:12 ..
AccI GT'mk_AC
Cuts at: 1348 1348
Size: 4310
Acc65I G'GTAC_C
Cuts at: 1326 1326
Size: 4310
AclI AA'CG_TT
Cuts at: 4150 4150
Size: 4310
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 215 510 2135 2567 3314 3582 215
Size: 295 1625 432 747 268 943
Fragments arranged by size:
1625 943 747 432 295 268
AflIII A'CryG_T
Cuts at: 443 1662 2766 3743 443
Size: 1219 1104 977 1010
Fragments arranged by size:
1219 1104 1010 977
AgeI A'CCGG_T
Cuts at: 2698 2698
Size: 4310
AleI CACnn'nnGTG
Cuts at: 838 838
Size: 4310
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 147 147
Size: 4310
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 953 985 953
Size: 32 4278
AlwNI CAG_nnn'CTG
Cuts at: 383 1615 3182 383
Size: 1232 1567 1511
Fragments arranged by size:
1567 1511 1232
ApaLI G'TGCA_C
Cuts at: 3080 3080
Size: 4310
ApoI r'AATT_y
Cuts at: 287 1164 1259 1314 3663 3919 287
Size: 877 95 55 2349 256 678
Fragments arranged by size:
2349 877 678 256 95 55
AseI AT'TA_AT
Cuts at: 20 20
Size: 4310
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4310
AvaI C'yCGr_G
Cuts at: 1330 1330
Size: 4310
BamHI G'GATC_C
Cuts at: 1335 1335
Size: 4310
BanI G'GyrC_C
Cuts at: 628 1326 1740 1988 2023 628
Size: 698 414 248 35 2915
Fragments arranged by size:
2915 698 414 248 35
BanII G_rGCy'C
Cuts at: 993 1324 2354 993
Size: 331 1030 2949
Fragments arranged by size:
2949 1030 331
BbeI G_GCGC'C
Cuts at: 1744 1992 1744
Size: 248 4062
BciVI GTATCCnnnnn_n'
Cuts at: 2208 2969 4310 2208
Size: 761 1341 2208
Fragments arranged by size:
2208 1341 761
BclI T'GATC_A
Cuts at: 1041 1830 1041
Size: 789 3521
BglII A'GATC_T
Cuts at: 1825 3862 1825
Size: 2037 2273
BlpI GC'TnA_GC
Cuts at: 1422 1422
Size: 4310
Bme1580I G_kGCm'C
Cuts at: 1935 2028 3084 1935
Size: 93 1056 3161
Fragments arranged by size:
3161 1056 93
BmgBI CAC'GTC
Cuts at: 444 444
Size: 4310
BmrI ACTGGGnnnn_n'
Cuts at: 240 528 996 1522 1603 1938 4082 240
Size: 288 468 526 81 335 2144 468
Fragments arranged by size:
2144 526 468 468 335 288 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 347 2677 347
Size: 2330 1980
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 689 689
Size: 4310
Bpu10I CC'TnA_GC
Cuts at: 992 992
Size: 4310
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1486 2365 2857 3155 1486
Size: 879 492 298 2641
Fragments arranged by size:
2641 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 4014 4014
Size: 4310
BsaAI yAC'GTr
Cuts at: 612 1663 612
Size: 1051 3259
BsaBI GATnn'nnATC
Cuts at: 6 1824 3861 6
Size: 1818 2037 455
Fragments arranged by size:
2037 1818 455
BsaHI Gr'CG_yC
Cuts at: 1741 1989 2691 4187 1741
Size: 248 702 1496 1864
Fragments arranged by size:
1864 1496 702 248
BsaWI w'CCGG_w
Cuts at: 510 1499 2020 2698 2972 3119 3530 510
Size: 989 521 678 274 147 411 1290
Fragments arranged by size:
1290 989 678 521 411 274 147
BseYI C'CCAG_C
Cuts at: 1735 2493 3070 3566 1735
Size: 758 577 496 2479
Fragments arranged by size:
2479 758 577 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 967 967
Size: 4310
BsiEI CG_ry'CG
Cuts at: 104 1852 1898 3106 104
Size: 1748 46 1208 1308
Fragments arranged by size:
1748 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 1324 2102 2292 3084 1324
Size: 778 190 792 2550
Fragments arranged by size:
2550 792 778 190
Bsp1286I G_dGCh'C
Cuts at: 993 1324 1935 2028 2102 2292 2354 3084 993
Size: 331 611 93 74 190 62 730 2219
Fragments arranged by size:
2219 730 611 331 190 93 74 62
BspEI T'CCGG_A
Cuts at: 3530 3530
Size: 4310
BspHI T'CATG_A
Cuts at: 3486 3486
Size: 4310
BspMI ACCTGCnnnn'nnnn_
Cuts at: 404 1360 1574 1876 2257 404
Size: 956 214 302 381 2457
Fragments arranged by size:
2457 956 381 302 214
BsrBI CCG'CTC
Cuts at: 50 2602 2656 4140 4305 50
Size: 2552 54 1484 165 55
Fragments arranged by size:
2552 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 827 2222 827
Size: 1395 2915
BsrFI r'CCGG_y
Cuts at: 2308 2489 2698 2308
Size: 181 209 3920
Fragments arranged by size:
3920 209 181
BssSI C'ACGA_G
Cuts at: 2581 2939 2581
Size: 358 3952
BstAPI GCAn_nnn'nTGC
Cuts at: 1687 1687
Size: 4310
BstBI TT'CG_AA
Cuts at: 2671 2671
Size: 4310
BstYI r'GATC_y
Cuts at: 1 151 1335 1802 1825 2160 2406 3418 3504
Size: 150 1184 467 23 335 246 1012 86
Cuts at: 3504 3516 3862 1
Size: 12 346 449
Fragments arranged by size:
1184 1012 467 449 346 335 246 150
86 23 12
Bsu36I CC'TnA_GG
Cuts at: 1253 1253
Size: 4310
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 2260 2441 2604 2260
Size: 181 163 3966
Fragments arranged by size:
3966 181 163
BtsI GCAGTG_nn'
Cuts at: 1553 1553
Size: 4310
DraI TTT'AAA
Cuts at: 1309 1309
Size: 4310
DrdI GACnn_nn'nnGTC
Cuts at: 2016 2874 2016
Size: 858 3452
EaeI y'GGCC_r
Cuts at: 1895 2069 2460 2487 3832 1895
Size: 174 391 27 1345 2373
Fragments arranged by size:
2373 1345 391 174 27
EagI C'GGCC_G
Cuts at: 1895 1895
Size: 4310
EarI CTCTTCn'nnn_
Cuts at: 242 303 2333 2543 242
Size: 61 2030 210 2009
Fragments arranged by size:
2030 2009 210 61
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2518 2828 2974 4120 2518
Size: 310 146 1146 2708
Fragments arranged by size:
2708 1146 310 146
EcoICRI GAG'CTC
Cuts at: 1322 1322
Size: 4310
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 215 510 689 2135 2567 3314 3582 215
Size: 295 179 1446 432 747 268 943
Fragments arranged by size:
1446 943 747 432 295 268 179
EcoNI CCTnn'n_nnAGG
Cuts at: 1240 1240
Size: 4310
EcoO109I rG'GnC_Cy
Cuts at: 1449 1449
Size: 4310
EcoRI G'AATT_C
Cuts at: 1314 1314
Size: 4310
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2329 2329
Size: 4310
FspI TGC'GCA
Cuts at: 2091 2091
Size: 4310
HaeII r_GCGC'y
Cuts at: 1744 1992 3014 3993 1744
Size: 248 1022 979 2061
Fragments arranged by size:
2061 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 2152 2182 2184 2214 2152
Size: 30 2 30 4248
Fragments arranged by size:
4248 30 30 2
HincII GTy'rAC
Cuts at: 1349 1349
Size: 4310
HindIII A'AGCT_T
Cuts at: 1019 1019
Size: 4310
KasI G'GCGC_C
Cuts at: 1740 1988 1740
Size: 248 4062
KpnI G_GTAC'C
Cuts at: 1330 1330
Size: 4310
MluI A'CGCG_T
Cuts at: 3743 3743
Size: 4310
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1132 2981 3165 1132
Size: 1849 184 2277
Fragments arranged by size:
2277 1849 184
MscI TGG'CCA
Cuts at: 2071 2071
Size: 4310
MslI CAynn'nnrTG
Cuts at: 838 2299 2426 838
Size: 1461 127 2722
Fragments arranged by size:
2722 1461 127
MspA1I CmG'CkG
Cuts at: 1421 1735 2095 3108 3353 1421
Size: 314 360 1013 245 2378
Fragments arranged by size:
2378 1013 360 314 245
NaeI GCC'GGC
Cuts at: 2491 2491
Size: 4310
NarI GG'CG_CC
Cuts at: 1741 1989 1741
Size: 248 4062
NgoMIV G'CCGG_C
Cuts at: 2489 2489
Size: 4310
NspI r_CATG'y
Cuts at: 134 1363 1863 2770 134
Size: 1229 500 907 1674
Fragments arranged by size:
1674 1229 907 500
PasI CC'CwG_GG
Cuts at: 319 319
Size: 4310
PciI A'CATG_T
Cuts at: 2766 2766
Size: 4310
PflMI CCAn_nnn'nTGG
Cuts at: 383 383
Size: 4310
PmeI GTTT'AAAC
Cuts at: 1309 1309
Size: 4310
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3449 3481 3449
Size: 32 4278
PstI C_TGCA'G
Cuts at: 1357 2042 1357
Size: 685 3625
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4310
PvuII CAG'CTG
Cuts at: 1735 2095 1735
Size: 360 3950
RsrII CG'GwC_CG
Cuts at: 2505 2505
Size: 4310
SacI G_AGCT'C
Cuts at: 1324 1324
Size: 4310
SalI G'TCGA_C
Cuts at: 1347 1347
Size: 4310
SapI GCTCTTCn'nnn_
Cuts at: 2333 2543 2333
Size: 210 4100
SbfI CC_TGCA'GG
Cuts at: 1357 1357
Size: 4310
ScaI AGT'ACT
Cuts at: 3827 3827
Size: 4310
SfcI C'TryA_G
Cuts at: 33 1353 2038 3031 3222 33
Size: 1320 685 993 191 1121
Fragments arranged by size:
1320 1121 993 685 191
SfoI GGC'GCC
Cuts at: 1742 1990 1742
Size: 248 4062
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4310
SmaI CCC'GGG
Cuts at: 1332 1332
Size: 4310
SmlI C'TyrA_G
Cuts at: 1465 2380 2872 3134 1465
Size: 915 492 262 2641
Fragments arranged by size:
2641 915 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4310
SphI G_CATG'C
Cuts at: 134 1363 1863 134
Size: 1229 500 2581
Fragments arranged by size:
2581 1229 500
SspI AAT'ATT
Cuts at: 210 1294 210
Size: 1084 3226
StyI C'CwwG_G
Cuts at: 282 1151 1236 1444 1798 282
Size: 869 85 208 354 2794
Fragments arranged by size:
2794 869 354 208 85
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 742 1893 2026 2485 2694 742
Size: 1151 133 459 209 2358
Fragments arranged by size:
2358 1151 459 209 133
TatI w'GTAC_w
Cuts at: 3825 3825
Size: 4310
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 717 749 3827 3859 717
Size: 32 3078 32 1168
Fragments arranged by size:
3078 1168 32 32
Tth111I GACn'n_nGTC
Cuts at: 2107 2107
Size: 4310
XbaI T'CTAG_A
Cuts at: 1341 1341
Size: 4310
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 408 1115 1805 408
Size: 707 690 2913
Fragments arranged by size:
2913 707 690
XmaI C'CCGG_G
Cuts at: 1330 1330
Size: 4310
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AgeI AleI AlfI
AloI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI
BanI BanII BbeI BciVI BclI BglII BlpI Bme1580I
BmgBI BmrI BplI BpmI Bpu10I BpuEI BsaI BsaAI
BsaBI BsaHI BsaWI BseYI BsgI BsiEI BsiHKAI Bsp1286I
BspEI BspHI BspMI BsrBI BsrDI BsrFI BssSI BstAPI
BstBI BstYI Bsu36I BtgZI BtsI DraI DrdI EaeI
EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI
FalI FspI HaeII Hin4I HincII HindIII KasI KpnI
MluI MmeI MscI MslI MspA1I NaeI NarI NgoMIV
NspI PasI PciI PflMI PmeI PpiI PstI PvuI
PvuII RsrII SacI SalI SapI SbfI ScaI SfcI
SfoI SgfI SmaI SmlI SpeI SphI SspI StyI
TaqII TatI TstI Tth111I XbaI XcmI XmaI
Enzymes that do not cut:
AarI AatII AfeI AflII AhdI ApaI AscI AvrII
BaeI BbsI BbvCI BcgI BfrBI BglI BmtI BsaXI
BseRI BsiWI BsmI BsmBI BsrGI BssHII BstEII BstXI
BstZ17I BtgI ClaI CspCI DraIII EcoRV FseI FspAI
HpaI MfeI NcoI NdeI NheI NotI NruI NsiI
PacI PfoI PmlI PpuMI PshAI PsiI PspOMI PspXI
PsrI SacII SanDI SexAI SfiI SgrAI SnaBI SrfI
StuI SwaI XhoI XmnI ZraI