Restriction map for
pF1KE0252
(Circular) (Six-base) MAPSORT of: pF1KE0252.seq Check: 9496 from: 1 to: 3764
pF1KE0252 3764 bp
With 182 enzymes: SgfI *
April 24, 2009 13:12 ..
AccI GT'mk_AC
Cuts at: 802 802
Size: 3764
Acc65I G'GTAC_C
Cuts at: 780 780
Size: 3764
AclI AA'CG_TT
Cuts at: 3604 3604
Size: 3764
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 259 1589 2021 2768 3036 259
Size: 1330 432 747 268 987
Fragments arranged by size:
1330 987 747 432 268
AflIII A'CryG_T
Cuts at: 432 1116 2220 3197 432
Size: 684 1104 977 999
Fragments arranged by size:
1104 999 977 684
AgeI A'CCGG_T
Cuts at: 2152 2152
Size: 3764
AleI CACnn'nnGTG
Cuts at: 201 201
Size: 3764
AlwNI CAG_nnn'CTG
Cuts at: 126 1069 2636 126
Size: 943 1567 1254
Fragments arranged by size:
1567 1254 943
ApaLI G'TGCA_C
Cuts at: 2534 2534
Size: 3764
ApoI r'AATT_y
Cuts at: 768 3117 3373 768
Size: 2349 256 1159
Fragments arranged by size:
2349 1159 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 3764
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 3764
AvaI C'yCGr_G
Cuts at: 784 784
Size: 3764
BamHI G'GATC_C
Cuts at: 789 789
Size: 3764
BanI G'GyrC_C
Cuts at: 129 203 780 1194 1442 1477 129
Size: 74 577 414 248 35 2416
Fragments arranged by size:
2416 577 414 248 74 35
BanII G_rGCy'C
Cuts at: 778 1808 778
Size: 1030 2734
BbeI G_GCGC'C
Cuts at: 1198 1446 1198
Size: 248 3516
BciVI GTATCCnnnnn_n'
Cuts at: 1662 2423 3764 1662
Size: 761 1341 1662
Fragments arranged by size:
1662 1341 761
BclI T'GATC_A
Cuts at: 1284 1284
Size: 3764
BglII A'GATC_T
Cuts at: 1279 3316 1279
Size: 2037 1727
BlpI GC'TnA_GC
Cuts at: 876 876
Size: 3764
Bme1580I G_kGCm'C
Cuts at: 134 208 1389 1482 2538 134
Size: 74 1181 93 1056 1360
Fragments arranged by size:
1360 1181 1056 93 74
BmrI ACTGGGnnnn_n'
Cuts at: 624 976 1057 1392 3536 624
Size: 352 81 335 2144 852
Fragments arranged by size:
2144 852 352 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2131 2131
Size: 3764
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 667 667
Size: 3764
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 940 1819 2311 2609 940
Size: 879 492 298 2095
Fragments arranged by size:
2095 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 3468 3468
Size: 3764
BsaAI yAC'GTr
Cuts at: 1117 1117
Size: 3764
BsaBI GATnn'nnATC
Cuts at: 6 1278 3315 6
Size: 1272 2037 455
Fragments arranged by size:
2037 1272 455
BsaHI Gr'CG_yC
Cuts at: 1195 1443 2145 3641 1195
Size: 248 702 1496 1318
Fragments arranged by size:
1496 1318 702 248
BsaWI w'CCGG_w
Cuts at: 953 1474 2152 2426 2573 2984 953
Size: 521 678 274 147 411 1733
Fragments arranged by size:
1733 678 521 411 274 147
BseYI C'CCAG_C
Cuts at: 1189 1947 2524 3020 1189
Size: 758 577 496 1933
Fragments arranged by size:
1933 758 577 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 139 139
Size: 3764
BsiEI CG_ry'CG
Cuts at: 104 1306 1352 2560 104
Size: 1202 46 1208 1308
Fragments arranged by size:
1308 1208 1202 46
BsiHKAI G_wGCw'C
Cuts at: 778 1556 1746 2538 778
Size: 778 190 792 2004
Fragments arranged by size:
2004 792 778 190
Bsp1286I G_dGCh'C
Cuts at: 134 208 778 1389 1482 1556 1746 1808 2538
Size: 74 570 611 93 74 190 62 730
Cuts at: 2538 134
Size: 1360
Fragments arranged by size:
1360 730 611 570 190 93 74 74
62
BspEI T'CCGG_A
Cuts at: 2984 2984
Size: 3764
BspHI T'CATG_A
Cuts at: 2940 2940
Size: 3764
BspMI ACCTGCnnnn'nnnn_
Cuts at: 538 814 1028 1330 1711 538
Size: 276 214 302 381 2591
Fragments arranged by size:
2591 381 302 276 214
BsrBI CCG'CTC
Cuts at: 50 2056 2110 3594 3759 50
Size: 2006 54 1484 165 55
Fragments arranged by size:
2006 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 494 1676 494
Size: 1182 2582
BsrFI r'CCGG_y
Cuts at: 1762 1943 2152 1762
Size: 181 209 3374
Fragments arranged by size:
3374 209 181
BssSI C'ACGA_G
Cuts at: 2035 2393 2035
Size: 358 3406
BstAPI GCAn_nnn'nTGC
Cuts at: 1141 1141
Size: 3764
BstBI TT'CG_AA
Cuts at: 2125 2125
Size: 3764
BstXI CCAn_nnnn'nTGG
Cuts at: 627 627
Size: 3764
BstYI r'GATC_y
Cuts at: 1 461 519 789 1256 1279 1614 1860 2872
Size: 460 58 270 467 23 335 246 1012
Cuts at: 2872 2958 2970 3316 1
Size: 86 12 346 449
Fragments arranged by size:
1012 467 460 449 346 335 270 246
86 58 23 12
BtgI C'CryG_G
Cuts at: 107 107
Size: 3764
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 1714 1895 2058 1714
Size: 181 163 3420
Fragments arranged by size:
3420 181 163
BtsI GCAGTG_nn'
Cuts at: 585 676 1007 585
Size: 91 331 3342
Fragments arranged by size:
3342 331 91
DraI TTT'AAA
Cuts at: 763 763
Size: 3764
DrdI GACnn_nn'nnGTC
Cuts at: 1470 2328 1470
Size: 858 2906
EaeI y'GGCC_r
Cuts at: 369 1349 1523 1914 1941 3286 369
Size: 980 174 391 27 1345 847
Fragments arranged by size:
1345 980 847 391 174 27
EagI C'GGCC_G
Cuts at: 1349 1349
Size: 3764
EarI CTCTTCn'nnn_
Cuts at: 1787 1997 1787
Size: 210 3554
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 1972 2282 2428 3574 1972
Size: 310 146 1146 2162
Fragments arranged by size:
2162 1146 310 146
EcoICRI GAG'CTC
Cuts at: 776 776
Size: 3764
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 259 667 1589 2021 2768 3036 259
Size: 408 922 432 747 268 987
Fragments arranged by size:
987 922 747 432 408 268
EcoO109I rG'GnC_Cy
Cuts at: 180 903 180
Size: 723 3041
EcoRI G'AATT_C
Cuts at: 768 768
Size: 3764
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 1783 1783
Size: 3764
FspI TGC'GCA
Cuts at: 1545 1545
Size: 3764
HaeII r_GCGC'y
Cuts at: 127 1198 1446 2468 3447 127
Size: 1071 248 1022 979 444
Fragments arranged by size:
1071 1022 979 444 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1606 1636 1638 1668 1606
Size: 30 2 30 3702
Fragments arranged by size:
3702 30 30 2
HincII GTy'rAC
Cuts at: 803 803
Size: 3764
KasI G'GCGC_C
Cuts at: 1194 1442 1194
Size: 248 3516
KpnI G_GTAC'C
Cuts at: 784 784
Size: 3764
MluI A'CGCG_T
Cuts at: 3197 3197
Size: 3764
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 2435 2619 2435
Size: 184 3580
MscI TGG'CCA
Cuts at: 1525 1525
Size: 3764
MslI CAynn'nnrTG
Cuts at: 201 731 1753 1880 201
Size: 530 1022 127 2085
Fragments arranged by size:
2085 1022 530 127
MspA1I CmG'CkG
Cuts at: 875 1189 1549 2562 2807 875
Size: 314 360 1013 245 1832
Fragments arranged by size:
1832 1013 360 314 245
NaeI GCC'GGC
Cuts at: 1945 1945
Size: 3764
NarI GG'CG_CC
Cuts at: 1195 1443 1195
Size: 248 3516
NcoI C'CATG_G
Cuts at: 107 107
Size: 3764
NgoMIV G'CCGG_C
Cuts at: 1943 1943
Size: 3764
NspI r_CATG'y
Cuts at: 582 817 1317 2224 582
Size: 235 500 907 2122
Fragments arranged by size:
2122 907 500 235
PciI A'CATG_T
Cuts at: 2220 2220
Size: 3764
PflMI CCAn_nnn'nTGG
Cuts at: 378 378
Size: 3764
PfoI T'CCnGG_A
Cuts at: 457 457
Size: 3764
PmeI GTTT'AAAC
Cuts at: 763 763
Size: 3764
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2903 2935 2903
Size: 32 3732
PpuMI rG'GwC_Cy
Cuts at: 180 180
Size: 3764
PsiI TTA'TAA
Cuts at: 271 271
Size: 3764
PstI C_TGCA'G
Cuts at: 811 1496 811
Size: 685 3079
PvuI CG_AT'CG
Cuts at: 104 104
Size: 3764
PvuII CAG'CTG
Cuts at: 1189 1549 1189
Size: 360 3404
RsrII CG'GwC_CG
Cuts at: 1959 1959
Size: 3764
SacI G_AGCT'C
Cuts at: 778 778
Size: 3764
SalI G'TCGA_C
Cuts at: 801 801
Size: 3764
SanDI GG'GwC_CC
Cuts at: 180 180
Size: 3764
SapI GCTCTTCn'nnn_
Cuts at: 1787 1997 1787
Size: 210 3554
SbfI CC_TGCA'GG
Cuts at: 811 811
Size: 3764
ScaI AGT'ACT
Cuts at: 3281 3281
Size: 3764
SfcI C'TryA_G
Cuts at: 33 279 807 1492 2485 2676 33
Size: 246 528 685 993 191 1121
Fragments arranged by size:
1121 993 685 528 246 191
SfoI GGC'GCC
Cuts at: 1196 1444 1196
Size: 248 3516
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 3764
SmaI CCC'GGG
Cuts at: 786 786
Size: 3764
SmlI C'TyrA_G
Cuts at: 919 1834 2326 2588 919
Size: 915 492 262 2095
Fragments arranged by size:
2095 915 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 3764
SphI G_CATG'C
Cuts at: 817 1317 817
Size: 500 3264
SspI AAT'ATT
Cuts at: 664 664
Size: 3764
StyI C'CwwG_G
Cuts at: 107 244 377 898 1252 107
Size: 137 133 521 354 2619
Fragments arranged by size:
2619 521 354 137 133
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 344 349 1347 1480 1939 2148 344
Size: 5 998 133 459 209 1960
Fragments arranged by size:
1960 998 459 209 133 5
TatI w'GTAC_w
Cuts at: 3279 3279
Size: 3764
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3281 3313 3281
Size: 32 3732
Tth111I GACn'n_nGTC
Cuts at: 1561 1561
Size: 3764
XbaI T'CTAG_A
Cuts at: 795 795
Size: 3764
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 165 1259 165
Size: 1094 2670
XmaI C'CCGG_G
Cuts at: 784 784
Size: 3764
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AgeI AleI AlwNI
ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII
BbeI BciVI BclI BglII BlpI Bme1580I BmrI BplI
BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BseYI
BsgI BsiEI BsiHKAI Bsp1286I BspEI BspHI BspMI BsrBI
BsrDI BsrFI BssSI BstAPI BstBI BstXI BstYI BtgI
BtgZI BtsI DraI DrdI EaeI EagI EarI EciI
EcoICRI Eco57MI EcoO109I EcoRI FalI FspI HaeII Hin4I
HincII KasI KpnI MluI MmeI MscI MslI MspA1I
NaeI NarI NcoI NgoMIV NspI PciI PflMI PfoI
PmeI PpiI PpuMI PsiI PstI PvuI PvuII RsrII
SacI SalI SanDI SapI SbfI ScaI SfcI SfoI
SgfI SmaI SmlI SpeI SphI SspI StyI TaqII
TatI TstI Tth111I XbaI XcmI XmaI
Enzymes that do not cut:
AarI AatII AfeI AflII AhdI AlfI AloI ApaI
AscI AvrII BaeI BbsI BbvCI BcgI BfrBI BglI
BmgBI BmtI Bpu10I BsaXI BseRI BsiWI BsmI BsmBI
BsrGI BssHII BstEII BstZ17I Bsu36I ClaI CspCI DraIII
EcoNI EcoRV FseI FspAI HindIII HpaI MfeI NdeI
NheI NotI NruI NsiI PacI PasI PmlI PshAI
PspOMI PspXI PsrI SacII SexAI SfiI SgrAI SnaBI
SrfI StuI SwaI XhoI XmnI ZraI