Restriction map for
pF1KE0248
(Circular) (Six-base) MAPSORT of: pF1KE0248.seq Check: 9778 from: 1 to: 3506
pF1KE0248 3506 bp
With 182 enzymes: SgfI *
April 24, 2009 13:12 ..
AccI GT'mk_AC
Cuts at: 544 544
Size: 3506
Acc65I G'GTAC_C
Cuts at: 522 522
Size: 3506
AclI AA'CG_TT
Cuts at: 3346 3346
Size: 3506
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1331 1763 2510 2778 1331
Size: 432 747 268 2059
Fragments arranged by size:
2059 747 432 268
AflIII A'CryG_T
Cuts at: 272 858 1962 2939 272
Size: 586 1104 977 839
Fragments arranged by size:
1104 977 839 586
AgeI A'CCGG_T
Cuts at: 1894 1894
Size: 3506
AlwNI CAG_nnn'CTG
Cuts at: 123 811 2378 123
Size: 688 1567 1251
Fragments arranged by size:
1567 1251 688
ApaLI G'TGCA_C
Cuts at: 2276 2276
Size: 3506
ApoI r'AATT_y
Cuts at: 510 2859 3115 510
Size: 2349 256 901
Fragments arranged by size:
2349 901 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 3506
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 3506
AvaI C'yCGr_G
Cuts at: 526 526
Size: 3506
BamHI G'GATC_C
Cuts at: 531 531
Size: 3506
BanI G'GyrC_C
Cuts at: 522 936 1184 1219 522
Size: 414 248 35 2809
Fragments arranged by size:
2809 414 248 35
BanII G_rGCy'C
Cuts at: 170 520 1550 170
Size: 350 1030 2126
Fragments arranged by size:
2126 1030 350
BbeI G_GCGC'C
Cuts at: 940 1188 940
Size: 248 3258
BbsI GAAGACnn'nnnn_
Cuts at: 352 366 352
Size: 14 3492
BciVI GTATCCnnnnn_n'
Cuts at: 1404 2165 3506 1404
Size: 761 1341 1404
Fragments arranged by size:
1404 1341 761
BclI T'GATC_A
Cuts at: 1026 1026
Size: 3506
BglII A'GATC_T
Cuts at: 1021 3058 1021
Size: 2037 1469
BlpI GC'TnA_GC
Cuts at: 618 618
Size: 3506
Bme1580I G_kGCm'C
Cuts at: 1131 1224 2280 1131
Size: 93 1056 2357
Fragments arranged by size:
2357 1056 93
BmgBI CAC'GTC
Cuts at: 447 447
Size: 3506
BmrI ACTGGGnnnn_n'
Cuts at: 406 718 799 1134 3278 406
Size: 312 81 335 2144 634
Fragments arranged by size:
2144 634 335 312 81
BmtI G_CTAG'C
Cuts at: 316 316
Size: 3506
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 1873 1873
Size: 3506
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 682 1561 2053 2351 682
Size: 879 492 298 1837
Fragments arranged by size:
1837 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 178 3210 178
Size: 3032 474
BsaAI yAC'GTr
Cuts at: 273 859 273
Size: 586 2920
BsaBI GATnn'nnATC
Cuts at: 6 1020 3057 6
Size: 1014 2037 455
Fragments arranged by size:
2037 1014 455
BsaHI Gr'CG_yC
Cuts at: 221 937 1185 1887 3383 221
Size: 716 248 702 1496 344
Fragments arranged by size:
1496 716 702 344 248
BsaWI w'CCGG_w
Cuts at: 243 405 695 1216 1894 2168 2315 2726 243
Size: 162 290 521 678 274 147 411 1023
Fragments arranged by size:
1023 678 521 411 290 274 162 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 130 160 316 346 130
Size: 30 156 30 3290
Fragments arranged by size:
3290 156 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 196 321 472 196
Size: 125 151 3230
Fragments arranged by size:
3230 151 125
BseYI C'CCAG_C
Cuts at: 931 1689 2266 2762 931
Size: 758 577 496 1675
Fragments arranged by size:
1675 758 577 496
BsiEI CG_ry'CG
Cuts at: 104 1048 1094 2302 104
Size: 944 46 1208 1308
Fragments arranged by size:
1308 1208 944 46
BsiHKAI G_wGCw'C
Cuts at: 151 170 520 1298 1488 2280 151
Size: 19 350 778 190 792 1377
Fragments arranged by size:
1377 792 778 350 190 19
BsmBI CGTCTCn'nnnn_
Cuts at: 228 228
Size: 3506
Bsp1286I G_dGCh'C
Cuts at: 151 170 520 1131 1224 1298 1488 1550 2280
Size: 19 350 611 93 74 190 62 730
Cuts at: 2280 151
Size: 1377
Fragments arranged by size:
1377 730 611 350 190 93 74 62
19
BspEI T'CCGG_A
Cuts at: 243 405 2726 243
Size: 162 2321 1023
Fragments arranged by size:
2321 1023 162
BspHI T'CATG_A
Cuts at: 2682 2682
Size: 3506
BspMI ACCTGCnnnn'nnnn_
Cuts at: 489 556 770 1072 1453 489
Size: 67 214 302 381 2542
Fragments arranged by size:
2542 381 302 214 67
BsrBI CCG'CTC
Cuts at: 50 1798 1852 3336 3501 50
Size: 1748 54 1484 165 55
Fragments arranged by size:
1748 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 1418 1418
Size: 3506
BsrFI r'CCGG_y
Cuts at: 1504 1685 1894 1504
Size: 181 209 3116
Fragments arranged by size:
3116 209 181
BssSI C'ACGA_G
Cuts at: 1777 2135 1777
Size: 358 3148
BstAPI GCAn_nnn'nTGC
Cuts at: 883 883
Size: 3506
BstBI TT'CG_AA
Cuts at: 1867 1867
Size: 3506
BstYI r'GATC_y
Cuts at: 1 531 998 1021 1356 1602 2614 2700 2712
Size: 530 467 23 335 246 1012 86 12
Cuts at: 2712 3058 1
Size: 346 449
Fragments arranged by size:
1012 530 467 449 346 335 246 86
23 12
BtgI C'CryG_G
Cuts at: 107 107
Size: 3506
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 156 1456 1637 1800 156
Size: 1300 181 163 1862
Fragments arranged by size:
1862 1300 181 163
BtsI GCAGTG_nn'
Cuts at: 749 749
Size: 3506
DraI TTT'AAA
Cuts at: 505 505
Size: 3506
DrdI GACnn_nn'nnGTC
Cuts at: 220 1212 2070 220
Size: 992 858 1656
Fragments arranged by size:
1656 992 858
EaeI y'GGCC_r
Cuts at: 125 1091 1265 1656 1683 3028 125
Size: 966 174 391 27 1345 603
Fragments arranged by size:
1345 966 603 391 174 27
EagI C'GGCC_G
Cuts at: 1091 1091
Size: 3506
EarI CTCTTCn'nnn_
Cuts at: 425 449 1529 1739 425
Size: 24 1080 210 2192
Fragments arranged by size:
2192 1080 210 24
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 484 1714 2024 2170 3316 484
Size: 1230 310 146 1146 674
Fragments arranged by size:
1230 1146 674 310 146
EcoICRI GAG'CTC
Cuts at: 168 518 168
Size: 350 3156
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1331 1763 2510 2778 1331
Size: 432 747 268 2059
Fragments arranged by size:
2059 747 432 268
EcoO109I rG'GnC_Cy
Cuts at: 645 645
Size: 3506
EcoRI G'AATT_C
Cuts at: 510 510
Size: 3506
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 1525 1525
Size: 3506
FspI TGC'GCA
Cuts at: 1287 1287
Size: 3506
HaeII r_GCGC'y
Cuts at: 940 1188 2210 3189 940
Size: 248 1022 979 1257
Fragments arranged by size:
1257 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 313 345 1348 1378 1380 1410 313
Size: 32 1003 30 2 30 2409
Fragments arranged by size:
2409 1003 32 30 30 2
HincII GTy'rAC
Cuts at: 545 545
Size: 3506
KasI G'GCGC_C
Cuts at: 936 1184 936
Size: 248 3258
KpnI G_GTAC'C
Cuts at: 526 526
Size: 3506
MluI A'CGCG_T
Cuts at: 2939 2939
Size: 3506
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 176 2177 2361 176
Size: 2001 184 1321
Fragments arranged by size:
2001 1321 184
MscI TGG'CCA
Cuts at: 1267 1267
Size: 3506
MslI CAynn'nnrTG
Cuts at: 1495 1622 1495
Size: 127 3379
MspA1I CmG'CkG
Cuts at: 123 617 931 1291 2304 2549 123
Size: 494 314 360 1013 245 1080
Fragments arranged by size:
1080 1013 494 360 314 245
NaeI GCC'GGC
Cuts at: 1687 1687
Size: 3506
NarI GG'CG_CC
Cuts at: 937 1185 937
Size: 248 3258
NcoI C'CATG_G
Cuts at: 107 107
Size: 3506
NgoMIV G'CCGG_C
Cuts at: 1685 1685
Size: 3506
NheI G'CTAG_C
Cuts at: 312 312
Size: 3506
NspI r_CATG'y
Cuts at: 559 1059 1966 559
Size: 500 907 2099
Fragments arranged by size:
2099 907 500
PciI A'CATG_T
Cuts at: 1962 1962
Size: 3506
PflMI CCAn_nnn'nTGG
Cuts at: 123 123
Size: 3506
PfoI T'CCnGG_A
Cuts at: 362 362
Size: 3506
PmeI GTTT'AAAC
Cuts at: 505 505
Size: 3506
PmlI CAC'GTG
Cuts at: 273 273
Size: 3506
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2645 2677 2645
Size: 32 3474
PstI C_TGCA'G
Cuts at: 553 1238 553
Size: 685 2821
PvuI CG_AT'CG
Cuts at: 104 104
Size: 3506
PvuII CAG'CTG
Cuts at: 123 931 1291 123
Size: 808 360 2338
Fragments arranged by size:
2338 808 360
RsrII CG'GwC_CG
Cuts at: 1701 1701
Size: 3506
SacI G_AGCT'C
Cuts at: 170 520 170
Size: 350 3156
SalI G'TCGA_C
Cuts at: 543 543
Size: 3506
SapI GCTCTTCn'nnn_
Cuts at: 1529 1739 1529
Size: 210 3296
SbfI CC_TGCA'GG
Cuts at: 553 553
Size: 3506
ScaI AGT'ACT
Cuts at: 3023 3023
Size: 3506
SfcI C'TryA_G
Cuts at: 33 549 1234 2227 2418 33
Size: 516 685 993 191 1121
Fragments arranged by size:
1121 993 685 516 191
SfoI GGC'GCC
Cuts at: 938 1186 938
Size: 248 3258
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 3506
SmaI CCC'GGG
Cuts at: 528 528
Size: 3506
SmlI C'TyrA_G
Cuts at: 661 1576 2068 2330 661
Size: 915 492 262 1837
Fragments arranged by size:
1837 915 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 3506
SphI G_CATG'C
Cuts at: 559 1059 559
Size: 500 3006
StyI C'CwwG_G
Cuts at: 107 640 994 107
Size: 533 354 2619
Fragments arranged by size:
2619 533 354
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 202 1089 1222 1681 1890 202
Size: 887 133 459 209 1818
Fragments arranged by size:
1818 887 459 209 133
TatI w'GTAC_w
Cuts at: 434 3021 434
Size: 2587 919
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 130 162 3023 3055 130
Size: 32 2861 32 581
Fragments arranged by size:
2861 581 32 32
Tth111I GACn'n_nGTC
Cuts at: 1303 1303
Size: 3506
XbaI T'CTAG_A
Cuts at: 537 537
Size: 3506
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1001 1001
Size: 3506
XmaI C'CCGG_G
Cuts at: 526 526
Size: 3506
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AgeI AlwNI ApaLI
ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI
BbsI BciVI BclI BglII BlpI Bme1580I BmgBI BmrI
BmtI BplI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BsaXI BseRI BseYI BsiEI BsiHKAI BsmBI Bsp1286I BspEI
BspHI BspMI BsrBI BsrDI BsrFI BssSI BstAPI BstBI
BstYI BtgI BtgZI BtsI DraI DrdI EaeI EagI
EarI EciI EcoICRI Eco57MI EcoO109I EcoRI FalI FspI
HaeII Hin4I HincII KasI KpnI MluI MmeI MscI
MslI MspA1I NaeI NarI NcoI NgoMIV NheI NspI
PciI PflMI PfoI PmeI PmlI PpiI PstI PvuI
PvuII RsrII SacI SalI SapI SbfI ScaI SfcI
SfoI SgfI SmaI SmlI SpeI SphI StyI TaqII
TatI TstI Tth111I XbaI XcmI XmaI
Enzymes that do not cut:
AarI AatII AfeI AflII AhdI AleI AlfI AloI
ApaI AscI AvrII BaeI BbvCI BcgI BfrBI BglI
BpmI Bpu10I BsgI BsiWI BsmI BsrGI BssHII BstEII
BstXI BstZ17I Bsu36I ClaI CspCI DraIII EcoNI EcoRV
FseI FspAI HindIII HpaI MfeI NdeI NotI NruI
NsiI PacI PasI PpuMI PshAI PsiI PspOMI PspXI
PsrI SacII SanDI SexAI SfiI SgrAI SnaBI SrfI
SspI StuI SwaI XhoI XmnI ZraI