Restriction map for
pF1KE0247
(Circular) (Six-base) MAPSORT of: pF1KE0247.seq Check: 2119 from: 1 to: 4964
pF1KE0247 4964 bp
With 182 enzymes: SgfI *
April 24, 2009 13:12 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 1591 1591
Size: 4964
AccI GT'mk_AC
Cuts at: 2002 2002
Size: 4964
Acc65I G'GTAC_C
Cuts at: 1980 1980
Size: 4964
AclI AA'CG_TT
Cuts at: 4804 4804
Size: 4964
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 110 140 197 266 725 800 1202 1253 1428
Size: 30 57 69 459 75 402 51 175
Cuts at: 1428 2789 3221 3968 4236 110
Size: 1361 432 747 268 838
Fragments arranged by size:
1361 838 747 459 432 402 268 175
75 69 57 51 30
AflIII A'CryG_T
Cuts at: 2316 3420 4397 2316
Size: 1104 977 2883
Fragments arranged by size:
2883 1104 977
AgeI A'CCGG_T
Cuts at: 3352 3352
Size: 4964
AlwNI CAG_nnn'CTG
Cuts at: 582 830 2269 3836 582
Size: 248 1439 1567 1710
Fragments arranged by size:
1710 1567 1439 248
ApaLI G'TGCA_C
Cuts at: 3734 3734
Size: 4964
ApoI r'AATT_y
Cuts at: 289 402 472 837 1004 1580 1695 1968 4317
Size: 113 70 365 167 576 115 273 2349
Cuts at: 4317 4573 289
Size: 256 680
Fragments arranged by size:
2349 680 576 365 273 256 167 115
113 70
AseI AT'TA_AT
Cuts at: 20 20
Size: 4964
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4964
AvaI C'yCGr_G
Cuts at: 1984 1984
Size: 4964
BamHI G'GATC_C
Cuts at: 783 1989 783
Size: 1206 3758
BanI G'GyrC_C
Cuts at: 1980 2394 2642 2677 1980
Size: 414 248 35 4267
Fragments arranged by size:
4267 414 248 35
BanII G_rGCy'C
Cuts at: 770 1978 3008 770
Size: 1208 1030 2726
Fragments arranged by size:
2726 1208 1030
BbeI G_GCGC'C
Cuts at: 2398 2646 2398
Size: 248 4716
BbvCI CC'TCA_GC
Cuts at: 354 602 1265 1536 354
Size: 248 663 271 3782
Fragments arranged by size:
3782 663 271 248
BciVI GTATCCnnnnn_n'
Cuts at: 2862 3623 4964 2862
Size: 761 1341 2862
Fragments arranged by size:
2862 1341 761
BclI T'GATC_A
Cuts at: 342 2484 342
Size: 2142 2822
BfrBI ATG'CAT
Cuts at: 528 528
Size: 4964
BglII A'GATC_T
Cuts at: 333 2479 4516 333
Size: 2146 2037 781
Fragments arranged by size:
2146 2037 781
BlpI GC'TnA_GC
Cuts at: 2076 2076
Size: 4964
Bme1580I G_kGCm'C
Cuts at: 2589 2682 3738 2589
Size: 93 1056 3815
Fragments arranged by size:
3815 1056 93
BmrI ACTGGGnnnn_n'
Cuts at: 227 2176 2257 2592 4736 227
Size: 1949 81 335 2144 455
Fragments arranged by size:
2144 1949 455 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 212 617 3331 212
Size: 405 2714 1845
Fragments arranged by size:
2714 1845 405
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 457 457
Size: 4964
Bpu10I CC'TnA_GC
Cuts at: 354 602 1265 1536 354
Size: 248 663 271 3782
Fragments arranged by size:
3782 663 271 248
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 715 2140 3019 3511 3809 715
Size: 1425 879 492 298 1870
Fragments arranged by size:
1870 1425 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 4668 4668
Size: 4964
BsaAI yAC'GTr
Cuts at: 2317 2317
Size: 4964
BsaBI GATnn'nnATC
Cuts at: 6 563 2478 4515 6
Size: 557 1915 2037 455
Fragments arranged by size:
2037 1915 557 455
BsaHI Gr'CG_yC
Cuts at: 2395 2643 3345 4841 2395
Size: 248 702 1496 2518
Fragments arranged by size:
2518 1496 702 248
BsaWI w'CCGG_w
Cuts at: 2153 2674 3352 3626 3773 4184 2153
Size: 521 678 274 147 411 2933
Fragments arranged by size:
2933 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1440 1470 1440
Size: 30 4934
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1086 1621 1086
Size: 535 4429
BseYI C'CCAG_C
Cuts at: 2389 3147 3724 4220 2389
Size: 758 577 496 3133
Fragments arranged by size:
3133 758 577 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1548 1548
Size: 4964
BsiEI CG_ry'CG
Cuts at: 104 2506 2552 3760 104
Size: 2402 46 1208 1308
Fragments arranged by size:
2402 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 1978 2756 2946 3738 1978
Size: 778 190 792 3204
Fragments arranged by size:
3204 792 778 190
BsmI GAATG_Cn'
Cuts at: 826 918 1844 1911 826
Size: 92 926 67 3879
Fragments arranged by size:
3879 926 92 67
Bsp1286I G_dGCh'C
Cuts at: 770 1978 2589 2682 2756 2946 3008 3738 770
Size: 1208 611 93 74 190 62 730 1996
Fragments arranged by size:
1996 1208 730 611 190 93 74 62
BspEI T'CCGG_A
Cuts at: 4184 4184
Size: 4964
BspHI T'CATG_A
Cuts at: 1185 4140 1185
Size: 2955 2009
BspMI ACCTGCnnnn'nnnn_
Cuts at: 418 1591 2014 2228 2530 2911 418
Size: 1173 423 214 302 381 2471
Fragments arranged by size:
2471 1173 423 381 302 214
BsrBI CCG'CTC
Cuts at: 50 3256 3310 4794 4959 50
Size: 3206 54 1484 165 55
Fragments arranged by size:
3206 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 2876 2876
Size: 4964
BsrFI r'CCGG_y
Cuts at: 2962 3143 3352 2962
Size: 181 209 4574
Fragments arranged by size:
4574 209 181
BssSI C'ACGA_G
Cuts at: 3235 3593 3235
Size: 358 4606
BstAPI GCAn_nnn'nTGC
Cuts at: 364 830 1915 2341 364
Size: 466 1085 426 2987
Fragments arranged by size:
2987 1085 466 426
BstBI TT'CG_AA
Cuts at: 3325 3325
Size: 4964
BstYI r'GATC_y
Cuts at: 1 333 783 942 1989 2456 2479 2814 3060
Size: 332 450 159 1047 467 23 335 246
Cuts at: 3060 4072 4158 4170 4516 1
Size: 1012 86 12 346 449
Fragments arranged by size:
1047 1012 467 450 449 346 335 332
246 159 86 23 12
Bsu36I CC'TnA_GG
Cuts at: 1569 1569
Size: 4964
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 2914 3095 3258 2914
Size: 181 163 4620
Fragments arranged by size:
4620 181 163
BtsI GCAGTG_nn'
Cuts at: 831 1560 2207 831
Size: 729 647 3588
Fragments arranged by size:
3588 729 647
ClaI AT'CG_AT
Cuts at: 860 860
Size: 4964
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 1818 1853 1818
Size: 35 4929
DraI TTT'AAA
Cuts at: 1963 1963
Size: 4964
DrdI GACnn_nn'nnGTC
Cuts at: 2670 3528 2670
Size: 858 4106
EaeI y'GGCC_r
Cuts at: 1086 2549 2723 3114 3141 4486 1086
Size: 1463 174 391 27 1345 1564
Fragments arranged by size:
1564 1463 1345 391 174 27
EagI C'GGCC_G
Cuts at: 2549 2549
Size: 4964
EarI CTCTTCn'nnn_
Cuts at: 1413 2987 3197 1413
Size: 1574 210 3180
Fragments arranged by size:
3180 1574 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 3172 3482 3628 4774 3172
Size: 310 146 1146 3362
Fragments arranged by size:
3362 1146 310 146
EcoICRI GAG'CTC
Cuts at: 1976 1976
Size: 4964
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 110 140 197 266 457 725 800 1202 1253
Size: 30 57 69 191 268 75 402 51
Cuts at: 1253 1428 2789 3221 3968 4236 110
Size: 175 1361 432 747 268 838
Fragments arranged by size:
1361 838 747 432 402 268 268 191
175 75 69 57 51 30
EcoO109I rG'GnC_Cy
Cuts at: 465 690 2103 465
Size: 225 1413 3326
Fragments arranged by size:
3326 1413 225
EcoRI G'AATT_C
Cuts at: 289 402 1695 1968 289
Size: 113 1293 273 3285
Fragments arranged by size:
3285 1293 273 113
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 337 691 2983 337
Size: 354 2292 2318
Fragments arranged by size:
2318 2292 354
FspI TGC'GCA
Cuts at: 2745 2745
Size: 4964
HaeII r_GCGC'y
Cuts at: 2398 2646 3668 4647 2398
Size: 248 1022 979 2715
Fragments arranged by size:
2715 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1450 1482 1715 1747 2806 2836 2838 2868 1450
Size: 32 233 32 1059 30 2 30 3546
Fragments arranged by size:
3546 1059 233 32 32 30 30 2
HincII GTy'rAC
Cuts at: 1469 2003 1469
Size: 534 4430
HindIII A'AGCT_T
Cuts at: 653 653
Size: 4964
KasI G'GCGC_C
Cuts at: 2394 2642 2394
Size: 248 4716
KpnI G_GTAC'C
Cuts at: 1984 1984
Size: 4964
MluI A'CGCG_T
Cuts at: 4397 4397
Size: 4964
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 394 3635 3819 394
Size: 3241 184 1539
Fragments arranged by size:
3241 1539 184
MscI TGG'CCA
Cuts at: 1088 2725 1088
Size: 1637 3327
MslI CAynn'nnrTG
Cuts at: 984 2953 3080 984
Size: 1969 127 2868
Fragments arranged by size:
2868 1969 127
MspA1I CmG'CkG
Cuts at: 286 2075 2389 2749 3762 4007 286
Size: 1789 314 360 1013 245 1243
Fragments arranged by size:
1789 1243 1013 360 314 245
NaeI GCC'GGC
Cuts at: 3145 3145
Size: 4964
NarI GG'CG_CC
Cuts at: 2395 2643 2395
Size: 248 4716
NgoMIV G'CCGG_C
Cuts at: 3143 3143
Size: 4964
NsiI A_TGCA'T
Cuts at: 530 530
Size: 4964
NspI r_CATG'y
Cuts at: 2017 2517 3424 2017
Size: 500 907 3557
Fragments arranged by size:
3557 907 500
PasI CC'CwG_GG
Cuts at: 462 462
Size: 4964
PciI A'CATG_T
Cuts at: 3420 3420
Size: 4964
PmeI GTTT'AAAC
Cuts at: 1963 1963
Size: 4964
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4103 4135 4103
Size: 32 4932
PpuMI rG'GwC_Cy
Cuts at: 690 690
Size: 4964
PshAI GACnn'nnGTC
Cuts at: 380 1679 380
Size: 1299 3665
PstI C_TGCA'G
Cuts at: 2011 2696 2011
Size: 685 4279
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4964
PvuII CAG'CTG
Cuts at: 286 2389 2749 286
Size: 2103 360 2501
Fragments arranged by size:
2501 2103 360
RsrII CG'GwC_CG
Cuts at: 3159 3159
Size: 4964
SacI G_AGCT'C
Cuts at: 1978 1978
Size: 4964
SalI G'TCGA_C
Cuts at: 2001 2001
Size: 4964
SapI GCTCTTCn'nnn_
Cuts at: 2987 3197 2987
Size: 210 4754
SbfI CC_TGCA'GG
Cuts at: 2011 2011
Size: 4964
ScaI AGT'ACT
Cuts at: 4481 4481
Size: 4964
SfcI C'TryA_G
Cuts at: 33 1427 1955 2007 2692 3685 3876 33
Size: 1394 528 52 685 993 191 1121
Fragments arranged by size:
1394 1121 993 685 528 191 52
SfoI GGC'GCC
Cuts at: 2396 2644 2396
Size: 248 4716
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4964
SmaI CCC'GGG
Cuts at: 1986 1986
Size: 4964
SmlI C'TyrA_G
Cuts at: 694 2119 3034 3526 3788 694
Size: 1425 915 492 262 1870
Fragments arranged by size:
1870 1425 915 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4964
SphI G_CATG'C
Cuts at: 2017 2517 2017
Size: 500 4464
StuI AGG'CCT
Cuts at: 1713 1713
Size: 4964
StyI C'CwwG_G
Cuts at: 2098 2452 2098
Size: 354 4610
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 475 1602 2547 2680 3139 3348 475
Size: 1127 945 133 459 209 2091
Fragments arranged by size:
2091 1127 945 459 209 133
TatI w'GTAC_w
Cuts at: 4479 4479
Size: 4964
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 1440 1472 4481 4513 1440
Size: 32 3009 32 1891
Fragments arranged by size:
3009 1891 32 32
Tth111I GACn'n_nGTC
Cuts at: 164 2761 164
Size: 2597 2367
XbaI T'CTAG_A
Cuts at: 1995 1995
Size: 4964
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1662 2459 1662
Size: 797 4167
XmaI C'CCGG_G
Cuts at: 1984 1984
Size: 4964
Enzymes that do cut:
AarI AccI Acc65I AclI AcuI AflIII AgeI AlwNI
ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII
BbeI BbvCI BciVI BclI BfrBI BglII BlpI Bme1580I
BmrI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI
BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BssSI BstAPI BstBI BstYI Bsu36I BtgZI BtsI ClaI
CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI
Eco57MI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII
HindIII KasI KpnI MluI MmeI MscI MslI MspA1I
NaeI NarI NgoMIV NsiI NspI PasI PciI PmeI
PpiI PpuMI PshAI PstI PvuI PvuII RsrII SacI
SalI SapI SbfI ScaI SfcI SfoI SgfI SmaI
SmlI SpeI SphI StuI StyI TaqII TatI TstI
Tth111I XbaI XcmI XmaI
Enzymes that do not cut:
AatII AfeI AflII AhdI AleI AlfI AloI ApaI
AscI AvrII BaeI BbsI BcgI BglI BmgBI BmtI
BsiWI BsmBI BsrGI BssHII BstEII BstXI BstZ17I BtgI
DraIII EcoNI EcoRV FseI FspAI HpaI MfeI NcoI
NdeI NheI NotI NruI PacI PflMI PfoI PmlI
PsiI PspOMI PspXI PsrI SacII SanDI SexAI SfiI
SgrAI SnaBI SrfI SspI SwaI XhoI XmnI ZraI