Restriction map for
pF1KE0241
(Circular) (Six-base) MAPSORT of: pF1KE0241.seq Check: 8101 from: 1 to: 4394
pF1KE0241 4394 bp
With 182 enzymes: SgfI *
April 24, 2009 13:12 ..
AccI GT'mk_AC
Cuts at: 1432 1432
Size: 4394
Acc65I G'GTAC_C
Cuts at: 308 1410 308
Size: 1102 3292
AclI AA'CG_TT
Cuts at: 4234 4234
Size: 4394
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 131 675 842 2219 2651 3398 3666 131
Size: 544 167 1377 432 747 268 859
Fragments arranged by size:
1377 859 747 544 432 268 167
AflIII A'CryG_T
Cuts at: 331 1746 2850 3827 331
Size: 1415 1104 977 898
Fragments arranged by size:
1415 1104 977 898
AgeI A'CCGG_T
Cuts at: 2782 2782
Size: 4394
AleI CACnn'nnGTG
Cuts at: 334 334
Size: 4394
AlwNI CAG_nnn'CTG
Cuts at: 1699 3266 1699
Size: 1567 2827
ApaLI G'TGCA_C
Cuts at: 3164 3164
Size: 4394
ApoI r'AATT_y
Cuts at: 1221 1398 3747 4003 1221
Size: 177 2349 256 1612
Fragments arranged by size:
2349 1612 256 177
AseI AT'TA_AT
Cuts at: 20 20
Size: 4394
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4394
AvaI C'yCGr_G
Cuts at: 1414 1414
Size: 4394
BamHI G'GATC_C
Cuts at: 1419 1419
Size: 4394
BanI G'GyrC_C
Cuts at: 308 1410 1824 2072 2107 308
Size: 1102 414 248 35 2595
Fragments arranged by size:
2595 1102 414 248 35
BanII G_rGCy'C
Cuts at: 1361 1408 2438 1361
Size: 47 1030 3317
Fragments arranged by size:
3317 1030 47
BbeI G_GCGC'C
Cuts at: 1828 2076 1828
Size: 248 4146
BbsI GAAGACnn'nnnn_
Cuts at: 161 681 938 1274 161
Size: 520 257 336 3281
Fragments arranged by size:
3281 520 336 257
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 215 249 215
Size: 34 4360
BciVI GTATCCnnnnn_n'
Cuts at: 2292 3053 4394 2292
Size: 761 1341 2292
Fragments arranged by size:
2292 1341 761
BclI T'GATC_A
Cuts at: 1914 1914
Size: 4394
BfrBI ATG'CAT
Cuts at: 451 451
Size: 4394
BglI GCCn_nnn'nGGC
Cuts at: 178 655 1371 178
Size: 477 716 3201
Fragments arranged by size:
3201 716 477
BglII A'GATC_T
Cuts at: 1909 3946 1909
Size: 2037 2357
BlpI GC'TnA_GC
Cuts at: 1506 1506
Size: 4394
Bme1580I G_kGCm'C
Cuts at: 2019 2112 3168 2019
Size: 93 1056 3245
Fragments arranged by size:
3245 1056 93
BmrI ACTGGGnnnn_n'
Cuts at: 1606 1687 2022 4166 1606
Size: 81 335 2144 1834
Fragments arranged by size:
2144 1834 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 814 2761 814
Size: 1947 2447
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1570 2449 2941 3239 1570
Size: 879 492 298 2725
Fragments arranged by size:
2725 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 514 4098 514
Size: 3584 810
BsaAI yAC'GTr
Cuts at: 332 1747 332
Size: 1415 2979
BsaBI GATnn'nnATC
Cuts at: 6 1908 3945 6
Size: 1902 2037 455
Fragments arranged by size:
2037 1902 455
BsaHI Gr'CG_yC
Cuts at: 1825 2073 2775 4271 1825
Size: 248 702 1496 1948
Fragments arranged by size:
1948 1496 702 248
BsaWI w'CCGG_w
Cuts at: 1583 2104 2782 3056 3203 3614 1583
Size: 521 678 274 147 411 2363
Fragments arranged by size:
2363 678 521 411 274 147
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 790 790
Size: 4394
BseYI C'CCAG_C
Cuts at: 1819 2577 3154 3650 1819
Size: 758 577 496 2563
Fragments arranged by size:
2563 758 577 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 255 255
Size: 4394
BsiEI CG_ry'CG
Cuts at: 104 885 1936 1982 3190 104
Size: 781 1051 46 1208 1308
Fragments arranged by size:
1308 1208 1051 781 46
BsiHKAI G_wGCw'C
Cuts at: 795 1097 1408 2186 2376 3168 795
Size: 302 311 778 190 792 2021
Fragments arranged by size:
2021 792 778 311 302 190
BsmI GAATG_Cn'
Cuts at: 571 571
Size: 4394
BsmBI CGTCTCn'nnnn_
Cuts at: 147 713 147
Size: 566 3828
Bsp1286I G_dGCh'C
Cuts at: 795 1097 1361 1408 2019 2112 2186 2376 2438
Size: 302 264 47 611 93 74 190 62
Cuts at: 2438 3168 795
Size: 730 2021
Fragments arranged by size:
2021 730 611 302 264 190 93 74
62 47
BspEI T'CCGG_A
Cuts at: 3614 3614
Size: 4394
BspHI T'CATG_A
Cuts at: 3570 3570
Size: 4394
BspMI ACCTGCnnnn'nnnn_
Cuts at: 1444 1658 1960 2341 1444
Size: 214 302 381 3497
Fragments arranged by size:
3497 381 302 214
BsrBI CCG'CTC
Cuts at: 50 2686 2740 4224 4389 50
Size: 2636 54 1484 165 55
Fragments arranged by size:
2636 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 2306 2306
Size: 4394
BsrFI r'CCGG_y
Cuts at: 2392 2573 2782 2392
Size: 181 209 4004
Fragments arranged by size:
4004 209 181
BssSI C'ACGA_G
Cuts at: 2665 3023 2665
Size: 358 4036
BstAPI GCAn_nnn'nTGC
Cuts at: 1771 1771
Size: 4394
BstBI TT'CG_AA
Cuts at: 2755 2755
Size: 4394
BstXI CCAn_nnnn'nTGG
Cuts at: 336 336
Size: 4394
BstYI r'GATC_y
Cuts at: 1 1419 1886 1909 2244 2490 3502 3588 3600
Size: 1418 467 23 335 246 1012 86 12
Cuts at: 3600 3946 1
Size: 346 449
Fragments arranged by size:
1418 1012 467 449 346 335 246 86
23 12
BtgI C'CryG_G
Cuts at: 107 176 435 107
Size: 69 259 4066
Fragments arranged by size:
4066 259 69
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 2344 2525 2688 2344
Size: 181 163 4050
Fragments arranged by size:
4050 181 163
BtsI GCAGTG_nn'
Cuts at: 120 1019 1637 120
Size: 899 618 2877
Fragments arranged by size:
2877 899 618
ClaI AT'CG_AT
Cuts at: 620 881 620
Size: 261 4133
DraI TTT'AAA
Cuts at: 1393 1393
Size: 4394
DraIII CAC_nnn'GTG
Cuts at: 332 774 332
Size: 442 3952
DrdI GACnn_nn'nnGTC
Cuts at: 2100 2958 2100
Size: 858 3536
EaeI y'GGCC_r
Cuts at: 170 261 647 1979 2153 2544 2571 3916 170
Size: 91 386 1332 174 391 27 1345 648
Fragments arranged by size:
1345 1332 648 391 386 174 91 27
EagI C'GGCC_G
Cuts at: 1979 1979
Size: 4394
EarI CTCTTCn'nnn_
Cuts at: 150 202 514 664 1097 2417 2627 150
Size: 52 312 150 433 1320 210 1917
Fragments arranged by size:
1917 1320 433 312 210 150 52
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2602 2912 3058 4204 2602
Size: 310 146 1146 2792
Fragments arranged by size:
2792 1146 310 146
EcoICRI GAG'CTC
Cuts at: 1406 1406
Size: 4394
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 131 675 842 2219 2651 3398 3666 131
Size: 544 167 1377 432 747 268 859
Fragments arranged by size:
1377 859 747 544 432 268 167
EcoO109I rG'GnC_Cy
Cuts at: 441 1364 1533 441
Size: 923 169 3302
Fragments arranged by size:
3302 923 169
EcoRI G'AATT_C
Cuts at: 1398 1398
Size: 4394
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2413 2413
Size: 4394
FspI TGC'GCA
Cuts at: 2175 2175
Size: 4394
HaeII r_GCGC'y
Cuts at: 1828 2076 3098 4077 1828
Size: 248 1022 979 2145
Fragments arranged by size:
2145 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 2236 2266 2268 2298 2236
Size: 30 2 30 4332
Fragments arranged by size:
4332 30 30 2
HincII GTy'rAC
Cuts at: 486 1433 486
Size: 947 3447
HindIII A'AGCT_T
Cuts at: 467 467
Size: 4394
KasI G'GCGC_C
Cuts at: 1824 2072 1824
Size: 248 4146
KpnI G_GTAC'C
Cuts at: 312 1414 312
Size: 1102 3292
MfeI C'AATT_G
Cuts at: 572 572
Size: 4394
MluI A'CGCG_T
Cuts at: 3827 3827
Size: 4394
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1361 3065 3249 1361
Size: 1704 184 2506
Fragments arranged by size:
2506 1704 184
MscI TGG'CCA
Cuts at: 172 263 649 2155 172
Size: 91 386 1506 2411
Fragments arranged by size:
2411 1506 386 91
MslI CAynn'nnrTG
Cuts at: 269 286 334 589 1024 1082 2383 2510 269
Size: 17 48 255 435 58 1301 127 2153
Fragments arranged by size:
2153 1301 435 255 127 58 48 17
MspA1I CmG'CkG
Cuts at: 1505 1819 2179 3192 3437 1505
Size: 314 360 1013 245 2462
Fragments arranged by size:
2462 1013 360 314 245
NaeI GCC'GGC
Cuts at: 2575 2575
Size: 4394
NarI GG'CG_CC
Cuts at: 1825 2073 1825
Size: 248 4146
NcoI C'CATG_G
Cuts at: 107 176 435 107
Size: 69 259 4066
Fragments arranged by size:
4066 259 69
NgoMIV G'CCGG_C
Cuts at: 2573 2573
Size: 4394
NsiI A_TGCA'T
Cuts at: 453 453
Size: 4394
NspI r_CATG'y
Cuts at: 1447 1947 2854 1447
Size: 500 907 2987
Fragments arranged by size:
2987 907 500
PciI A'CATG_T
Cuts at: 2850 2850
Size: 4394
PflMI CCAn_nnn'nTGG
Cuts at: 774 774
Size: 4394
PmeI GTTT'AAAC
Cuts at: 1393 1393
Size: 4394
PmlI CAC'GTG
Cuts at: 332 332
Size: 4394
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3533 3565 3533
Size: 32 4362
PpuMI rG'GwC_Cy
Cuts at: 441 441
Size: 4394
PshAI GACnn'nnGTC
Cuts at: 685 685
Size: 4394
PstI C_TGCA'G
Cuts at: 1441 2126 1441
Size: 685 3709
PvuI CG_AT'CG
Cuts at: 104 885 104
Size: 781 3613
PvuII CAG'CTG
Cuts at: 1819 2179 1819
Size: 360 4034
RsrII CG'GwC_CG
Cuts at: 2589 2589
Size: 4394
SacI G_AGCT'C
Cuts at: 1408 1408
Size: 4394
SalI G'TCGA_C
Cuts at: 1431 1431
Size: 4394
SapI GCTCTTCn'nnn_
Cuts at: 2417 2627 2417
Size: 210 4184
SbfI CC_TGCA'GG
Cuts at: 1441 1441
Size: 4394
ScaI AGT'ACT
Cuts at: 3911 3911
Size: 4394
SfcI C'TryA_G
Cuts at: 33 132 911 1437 2122 3115 3306 33
Size: 99 779 526 685 993 191 1121
Fragments arranged by size:
1121 993 779 685 526 191 99
SfoI GGC'GCC
Cuts at: 1826 2074 1826
Size: 248 4146
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4394
SmaI CCC'GGG
Cuts at: 1416 1416
Size: 4394
SmlI C'TyrA_G
Cuts at: 1549 2464 2956 3218 1549
Size: 915 492 262 2725
Fragments arranged by size:
2725 915 492 262
SpeI A'CTAG_T
Cuts at: 67 1300 67
Size: 1233 3161
SphI G_CATG'C
Cuts at: 1447 1947 1447
Size: 500 3894
StyI C'CwwG_G
Cuts at: 107 176 435 1528 1882 107
Size: 69 259 1093 354 2619
Fragments arranged by size:
2619 1093 354 259 69
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 280 1977 2110 2569 2778 280
Size: 1697 133 459 209 1896
Fragments arranged by size:
1896 1697 459 209 133
TatI w'GTAC_w
Cuts at: 3909 3909
Size: 4394
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3911 3943 3911
Size: 32 4362
Tth111I GACn'n_nGTC
Cuts at: 2191 2191
Size: 4394
XbaI T'CTAG_A
Cuts at: 1353 1425 1353
Size: 72 4322
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1889 1889
Size: 4394
XmaI C'CCGG_G
Cuts at: 1414 1414
Size: 4394
XmnI GAAnn'nnTTC
Cuts at: 816 816
Size: 4394
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AgeI AleI AlwNI
ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII
BbeI BbsI BcgI BciVI BclI BfrBI BglI BglII
BlpI Bme1580I BmrI BplI BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BseRI BseYI BsgI BsiEI BsiHKAI BsmI
BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BssSI BstAPI BstBI BstXI BstYI BtgI BtgZI BtsI
ClaI DraI DraIII DrdI EaeI EagI EarI EciI
EcoICRI Eco57MI EcoO109I EcoRI FalI FspI HaeII Hin4I
HincII HindIII KasI KpnI MfeI MluI MmeI MscI
MslI MspA1I NaeI NarI NcoI NgoMIV NsiI NspI
PciI PflMI PmeI PmlI PpiI PpuMI PshAI PstI
PvuI PvuII RsrII SacI SalI SapI SbfI ScaI
SfcI SfoI SgfI SmaI SmlI SpeI SphI StyI
TaqII TatI TstI Tth111I XbaI XcmI XmaI XmnI
Enzymes that do not cut:
AarI AatII AfeI AflII AhdI AlfI AloI ApaI
AscI AvrII BaeI BbvCI BmgBI BmtI BpmI Bpu10I
BsaXI BsiWI BsrGI BssHII BstEII BstZ17I Bsu36I CspCI
EcoNI EcoRV FseI FspAI HpaI NdeI NheI NotI
NruI PacI PasI PfoI PsiI PspOMI PspXI PsrI
SacII SanDI SexAI SfiI SgrAI SnaBI SrfI SspI
StuI SwaI XhoI ZraI