Restriction map for
pF1KE0235
(Circular) (Six-base) MAPSORT of: pF1KE0235.seq Check: 6826 from: 1 to: 4052
pF1KE0235 4052 bp
With 182 enzymes: SgfI *
April 24, 2009 13:12 ..
AccI GT'mk_AC
Cuts at: 1090 1090
Size: 4052
Acc65I G'GTAC_C
Cuts at: 1068 1068
Size: 4052
AclI AA'CG_TT
Cuts at: 3892 3892
Size: 4052
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1877 2309 3056 3324 1877
Size: 432 747 268 2605
Fragments arranged by size:
2605 747 432 268
AflIII A'CryG_T
Cuts at: 1029 1404 2508 3485 1029
Size: 375 1104 977 1596
Fragments arranged by size:
1596 1104 977 375
AgeI A'CCGG_T
Cuts at: 2440 2440
Size: 4052
AlwNI CAG_nnn'CTG
Cuts at: 1357 2924 1357
Size: 1567 2485
ApaI G_GGCC'C
Cuts at: 892 892
Size: 4052
ApaLI G'TGCA_C
Cuts at: 2822 2822
Size: 4052
ApoI r'AATT_y
Cuts at: 300 1056 3405 3661 300
Size: 756 2349 256 691
Fragments arranged by size:
2349 756 691 256
AseI AT'TA_AT
Cuts at: 20 541 689 20
Size: 521 148 3383
Fragments arranged by size:
3383 521 148
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4052
AvaI C'yCGr_G
Cuts at: 1072 1072
Size: 4052
BamHI G'GATC_C
Cuts at: 1077 1077
Size: 4052
BanI G'GyrC_C
Cuts at: 1068 1482 1730 1765 1068
Size: 414 248 35 3355
Fragments arranged by size:
3355 414 248 35
BanII G_rGCy'C
Cuts at: 837 892 1066 2096 837
Size: 55 174 1030 2793
Fragments arranged by size:
2793 1030 174 55
BbeI G_GCGC'C
Cuts at: 1486 1734 1486
Size: 248 3804
BciVI GTATCCnnnnn_n'
Cuts at: 1950 2711 4052 1950
Size: 761 1341 1950
Fragments arranged by size:
1950 1341 761
BclI T'GATC_A
Cuts at: 912 1572 912
Size: 660 3392
BglII A'GATC_T
Cuts at: 1567 3604 1567
Size: 2037 2015
BlpI GC'TnA_GC
Cuts at: 1164 1164
Size: 4052
Bme1580I G_kGCm'C
Cuts at: 892 1677 1770 2826 892
Size: 785 93 1056 2118
Fragments arranged by size:
2118 1056 785 93
BmrI ACTGGGnnnn_n'
Cuts at: 1264 1345 1680 3824 1264
Size: 81 335 2144 1492
Fragments arranged by size:
2144 1492 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 856 2419 856
Size: 1563 2489
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1228 2107 2599 2897 1228
Size: 879 492 298 2383
Fragments arranged by size:
2383 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 3756 3756
Size: 4052
BsaAI yAC'GTr
Cuts at: 1405 1405
Size: 4052
BsaBI GATnn'nnATC
Cuts at: 6 1566 3603 6
Size: 1560 2037 455
Fragments arranged by size:
2037 1560 455
BsaHI Gr'CG_yC
Cuts at: 1483 1731 2433 3929 1483
Size: 248 702 1496 1606
Fragments arranged by size:
1606 1496 702 248
BsaWI w'CCGG_w
Cuts at: 1241 1762 2440 2714 2861 3272 1241
Size: 521 678 274 147 411 2021
Fragments arranged by size:
2021 678 521 411 274 147
BseYI C'CCAG_C
Cuts at: 1477 2235 2812 3308 1477
Size: 758 577 496 2221
Fragments arranged by size:
2221 758 577 496
BsiEI CG_ry'CG
Cuts at: 104 1594 1640 2848 104
Size: 1490 46 1208 1308
Fragments arranged by size:
1490 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 1066 1844 2034 2826 1066
Size: 778 190 792 2292
Fragments arranged by size:
2292 792 778 190
BsmI GAATG_Cn'
Cuts at: 196 400 196
Size: 204 3848
Bsp1286I G_dGCh'C
Cuts at: 837 892 1066 1677 1770 1844 2034 2096 2826
Size: 55 174 611 93 74 190 62 730
Cuts at: 2826 837
Size: 2063
Fragments arranged by size:
2063 730 611 190 174 93 74 62
55
BspEI T'CCGG_A
Cuts at: 3272 3272
Size: 4052
BspHI T'CATG_A
Cuts at: 3228 3228
Size: 4052
BspMI ACCTGCnnnn'nnnn_
Cuts at: 1102 1316 1618 1999 1102
Size: 214 302 381 3155
Fragments arranged by size:
3155 381 302 214
BsrBI CCG'CTC
Cuts at: 50 2344 2398 3882 4047 50
Size: 2294 54 1484 165 55
Fragments arranged by size:
2294 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 127 1964 127
Size: 1837 2215
BsrFI r'CCGG_y
Cuts at: 2050 2231 2440 2050
Size: 181 209 3662
Fragments arranged by size:
3662 209 181
BssSI C'ACGA_G
Cuts at: 2323 2681 2323
Size: 358 3694
BstAPI GCAn_nnn'nTGC
Cuts at: 1429 1429
Size: 4052
BstBI TT'CG_AA
Cuts at: 2413 2413
Size: 4052
BstYI r'GATC_y
Cuts at: 1 1077 1544 1567 1902 2148 3160 3246 3258
Size: 1076 467 23 335 246 1012 86 12
Cuts at: 3258 3604 1
Size: 346 449
Fragments arranged by size:
1076 1012 467 449 346 335 246 86
23 12
BtgI C'CryG_G
Cuts at: 107 434 107
Size: 327 3725
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 2002 2183 2346 2002
Size: 181 163 3708
Fragments arranged by size:
3708 181 163
BtsI GCAGTG_nn'
Cuts at: 1295 1295
Size: 4052
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 154 189 154
Size: 35 4017
DraI TTT'AAA
Cuts at: 1051 1051
Size: 4052
DrdI GACnn_nn'nnGTC
Cuts at: 1758 2616 1758
Size: 858 3194
EaeI y'GGCC_r
Cuts at: 1637 1811 2202 2229 3574 1637
Size: 174 391 27 1345 2115
Fragments arranged by size:
2115 1345 391 174 27
EagI C'GGCC_G
Cuts at: 1637 1637
Size: 4052
EarI CTCTTCn'nnn_
Cuts at: 107 778 847 880 2075 2285 107
Size: 671 69 33 1195 210 1874
Fragments arranged by size:
1874 1195 671 210 69 33
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2260 2570 2716 3862 2260
Size: 310 146 1146 2450
Fragments arranged by size:
2450 1146 310 146
EcoICRI GAG'CTC
Cuts at: 1064 1064
Size: 4052
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1877 2309 3056 3324 1877
Size: 432 747 268 2605
Fragments arranged by size:
2605 747 432 268
EcoO109I rG'GnC_Cy
Cuts at: 889 1191 889
Size: 302 3750
EcoRI G'AATT_C
Cuts at: 1056 1056
Size: 4052
EcoRV GAT'ATC
Cuts at: 326 383 326
Size: 57 3995
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2071 2071
Size: 4052
FspI TGC'GCA
Cuts at: 1833 1833
Size: 4052
HaeII r_GCGC'y
Cuts at: 1486 1734 2756 3735 1486
Size: 248 1022 979 1803
Fragments arranged by size:
1803 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 369 401 515 547 857 889 1894 1924 1926
Size: 32 114 32 310 32 1005 30 2
Cuts at: 1926 1956 369
Size: 30 2465
Fragments arranged by size:
2465 1005 310 114 32 32 32 30
30 2
HincII GTy'rAC
Cuts at: 1091 1091
Size: 4052
KasI G'GCGC_C
Cuts at: 1482 1730 1482
Size: 248 3804
KpnI G_GTAC'C
Cuts at: 1072 1072
Size: 4052
MluI A'CGCG_T
Cuts at: 3485 3485
Size: 4052
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 644 2723 2907 644
Size: 2079 184 1789
Fragments arranged by size:
2079 1789 184
MscI TGG'CCA
Cuts at: 1813 1813
Size: 4052
MslI CAynn'nnrTG
Cuts at: 223 883 1015 2041 2168 223
Size: 660 132 1026 127 2107
Fragments arranged by size:
2107 1026 660 132 127
MspA1I CmG'CkG
Cuts at: 1163 1477 1837 2850 3095 1163
Size: 314 360 1013 245 2120
Fragments arranged by size:
2120 1013 360 314 245
NaeI GCC'GGC
Cuts at: 2233 2233
Size: 4052
NarI GG'CG_CC
Cuts at: 1483 1731 1483
Size: 248 3804
NcoI C'CATG_G
Cuts at: 107 107
Size: 4052
NdeI CA'TA_TG
Cuts at: 465 484 552 465
Size: 19 68 3965
Fragments arranged by size:
3965 68 19
NgoMIV G'CCGG_C
Cuts at: 2231 2231
Size: 4052
NspI r_CATG'y
Cuts at: 1033 1105 1605 2512 1033
Size: 72 500 907 2573
Fragments arranged by size:
2573 907 500 72
PciI A'CATG_T
Cuts at: 1029 2508 1029
Size: 1479 2573
PmeI GTTT'AAAC
Cuts at: 1051 1051
Size: 4052
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3191 3223 3191
Size: 32 4020
PspOMI G'GGCC_C
Cuts at: 888 888
Size: 4052
PstI C_TGCA'G
Cuts at: 1099 1784 1099
Size: 685 3367
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4052
PvuII CAG'CTG
Cuts at: 1477 1837 1477
Size: 360 3692
RsrII CG'GwC_CG
Cuts at: 2247 2247
Size: 4052
SacI G_AGCT'C
Cuts at: 1066 1066
Size: 4052
SalI G'TCGA_C
Cuts at: 1089 1089
Size: 4052
SapI GCTCTTCn'nnn_
Cuts at: 2075 2285 2075
Size: 210 3842
SbfI CC_TGCA'GG
Cuts at: 1099 1099
Size: 4052
ScaI AGT'ACT
Cuts at: 3569 3569
Size: 4052
SfcI C'TryA_G
Cuts at: 33 975 1095 1780 2773 2964 33
Size: 942 120 685 993 191 1121
Fragments arranged by size:
1121 993 942 685 191 120
SfoI GGC'GCC
Cuts at: 1484 1732 1484
Size: 248 3804
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4052
SmaI CCC'GGG
Cuts at: 1074 1074
Size: 4052
SmlI C'TyrA_G
Cuts at: 1207 2122 2614 2876 1207
Size: 915 492 262 2383
Fragments arranged by size:
2383 915 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4052
SphI G_CATG'C
Cuts at: 1105 1605 1105
Size: 500 3552
StyI C'CwwG_G
Cuts at: 107 294 1186 1540 107
Size: 187 892 354 2619
Fragments arranged by size:
2619 892 354 187
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1635 1768 2227 2436 1635
Size: 133 459 209 3251
Fragments arranged by size:
3251 459 209 133
TatI w'GTAC_w
Cuts at: 284 627 673 3567 284
Size: 343 46 2894 769
Fragments arranged by size:
2894 769 343 46
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3569 3601 3569
Size: 32 4020
Tth111I GACn'n_nGTC
Cuts at: 1849 1849
Size: 4052
XbaI T'CTAG_A
Cuts at: 1083 1083
Size: 4052
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1547 1547
Size: 4052
XmaI C'CCGG_G
Cuts at: 1072 1072
Size: 4052
XmnI GAAnn'nnTTC
Cuts at: 358 443 358
Size: 85 3967
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AgeI AlwNI ApaI
ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII
BbeI BciVI BclI BglII BlpI Bme1580I BmrI BplI
BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BseYI BsiEI
BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI
BsrFI BssSI BstAPI BstBI BstYI BtgI BtgZI BtsI
CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI
Eco57MI EcoO109I EcoRI EcoRV FalI FspI HaeII Hin4I
HincII KasI KpnI MluI MmeI MscI MslI MspA1I
NaeI NarI NcoI NdeI NgoMIV NspI PciI PmeI
PpiI PspOMI PstI PvuI PvuII RsrII SacI SalI
SapI SbfI ScaI SfcI SfoI SgfI SmaI SmlI
SpeI SphI StyI TaqII TatI TstI Tth111I XbaI
XcmI XmaI XmnI
Enzymes that do not cut:
AarI AatII AfeI AflII AhdI AleI AlfI AloI
AscI AvrII BaeI BbsI BbvCI BcgI BfrBI BglI
BmgBI BmtI BpmI Bpu10I BsaXI BseRI BsgI BsiWI
BsmBI BsrGI BssHII BstEII BstXI BstZ17I Bsu36I ClaI
DraIII EcoNI FseI FspAI HindIII HpaI MfeI NheI
NotI NruI NsiI PacI PasI PflMI PfoI PmlI
PpuMI PshAI PsiI PspXI PsrI SacII SanDI SexAI
SfiI SgrAI SnaBI SrfI SspI StuI SwaI XhoI
ZraI