Restriction map for
pF1KE0231
(Circular) (Six-base) MAPSORT of: pF1KE0231.seq Check: 9875 from: 1 to: 4052
pF1KE0231 4052 bp
With 182 enzymes: SgfI *
April 24, 2009 13:12 ..
AccI GT'mk_AC
Cuts at: 737 1090 737
Size: 353 3699
Acc65I G'GTAC_C
Cuts at: 1068 1068
Size: 4052
AclI AA'CG_TT
Cuts at: 3892 3892
Size: 4052
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1877 2309 3056 3324 1877
Size: 432 747 268 2605
Fragments arranged by size:
2605 747 432 268
AflII C'TTAA_G
Cuts at: 814 814
Size: 4052
AflIII A'CryG_T
Cuts at: 1404 2508 3485 1404
Size: 1104 977 1971
Fragments arranged by size:
1971 1104 977
AgeI A'CCGG_T
Cuts at: 2440 2440
Size: 4052
AleI CACnn'nnGTG
Cuts at: 845 845
Size: 4052
AlwNI CAG_nnn'CTG
Cuts at: 1357 2924 1357
Size: 1567 2485
ApaI G_GGCC'C
Cuts at: 287 287
Size: 4052
ApaLI G'TGCA_C
Cuts at: 2822 2822
Size: 4052
ApoI r'AATT_y
Cuts at: 415 1056 3405 3661 415
Size: 641 2349 256 806
Fragments arranged by size:
2349 806 641 256
AseI AT'TA_AT
Cuts at: 20 926 20
Size: 906 3146
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4052
AvaI C'yCGr_G
Cuts at: 1072 1072
Size: 4052
BamHI G'GATC_C
Cuts at: 1077 1077
Size: 4052
BanI G'GyrC_C
Cuts at: 241 1068 1482 1730 1765 241
Size: 827 414 248 35 2528
Fragments arranged by size:
2528 827 414 248 35
BanII G_rGCy'C
Cuts at: 272 287 1066 2096 272
Size: 15 779 1030 2228
Fragments arranged by size:
2228 1030 779 15
BbeI G_GCGC'C
Cuts at: 1486 1734 1486
Size: 248 3804
BciVI GTATCCnnnnn_n'
Cuts at: 1950 2711 4052 1950
Size: 761 1341 1950
Fragments arranged by size:
1950 1341 761
BclI T'GATC_A
Cuts at: 461 854 1572 461
Size: 393 718 2941
Fragments arranged by size:
2941 718 393
BglII A'GATC_T
Cuts at: 1567 3604 1567
Size: 2037 2015
BlpI GC'TnA_GC
Cuts at: 1164 1164
Size: 4052
Bme1580I G_kGCm'C
Cuts at: 287 1677 1770 2826 287
Size: 1390 93 1056 1513
Fragments arranged by size:
1513 1390 1056 93
BmrI ACTGGGnnnn_n'
Cuts at: 1264 1345 1680 3824 1264
Size: 81 335 2144 1492
Fragments arranged by size:
2144 1492 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2419 2419
Size: 4052
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 463 463
Size: 4052
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 630 760 1228 2107 2599 2897 630
Size: 130 468 879 492 298 1785
Fragments arranged by size:
1785 879 492 468 298 130
BsaI GGTCTCn'nnnn_
Cuts at: 3756 3756
Size: 4052
BsaAI yAC'GTr
Cuts at: 1405 1405
Size: 4052
BsaBI GATnn'nnATC
Cuts at: 6 196 1566 3603 6
Size: 190 1370 2037 455
Fragments arranged by size:
2037 1370 455 190
BsaHI Gr'CG_yC
Cuts at: 1483 1731 2433 3929 1483
Size: 248 702 1496 1606
Fragments arranged by size:
1606 1496 702 248
BsaWI w'CCGG_w
Cuts at: 1241 1762 2440 2714 2861 3272 1241
Size: 521 678 274 147 411 2021
Fragments arranged by size:
2021 678 521 411 274 147
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 823 823
Size: 4052
BseYI C'CCAG_C
Cuts at: 1477 2235 2812 3308 1477
Size: 758 577 496 2221
Fragments arranged by size:
2221 758 577 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 869 869
Size: 4052
BsiEI CG_ry'CG
Cuts at: 104 1594 1640 2848 104
Size: 1490 46 1208 1308
Fragments arranged by size:
1490 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 723 1066 1844 2034 2826 723
Size: 343 778 190 792 1949
Fragments arranged by size:
1949 792 778 343 190
Bsp1286I G_dGCh'C
Cuts at: 272 287 723 1066 1677 1770 1844 2034 2096
Size: 15 436 343 611 93 74 190 62
Cuts at: 2096 2826 272
Size: 730 1498
Fragments arranged by size:
1498 730 611 436 343 190 93 74
62 15
BspEI T'CCGG_A
Cuts at: 3272 3272
Size: 4052
BspHI T'CATG_A
Cuts at: 665 3228 665
Size: 2563 1489
BspMI ACCTGCnnnn'nnnn_
Cuts at: 875 1102 1316 1618 1999 875
Size: 227 214 302 381 2928
Fragments arranged by size:
2928 381 302 227 214
BsrBI CCG'CTC
Cuts at: 50 2344 2398 3882 4047 50
Size: 2294 54 1484 165 55
Fragments arranged by size:
2294 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 749 1964 749
Size: 1215 2837
BsrFI r'CCGG_y
Cuts at: 2050 2231 2440 2050
Size: 181 209 3662
Fragments arranged by size:
3662 209 181
BssSI C'ACGA_G
Cuts at: 2323 2681 2323
Size: 358 3694
BstAPI GCAn_nnn'nTGC
Cuts at: 1429 1429
Size: 4052
BstBI TT'CG_AA
Cuts at: 2413 2413
Size: 4052
BstYI r'GATC_y
Cuts at: 1 191 1077 1544 1567 1902 2148 3160 3246
Size: 190 886 467 23 335 246 1012 86
Cuts at: 3246 3258 3604 1
Size: 12 346 449
Fragments arranged by size:
1012 886 467 449 346 335 246 190
86 23 12
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 2002 2183 2346 2002
Size: 181 163 3708
Fragments arranged by size:
3708 181 163
BtsI GCAGTG_nn'
Cuts at: 857 1295 857
Size: 438 3614
DraI TTT'AAA
Cuts at: 1051 1051
Size: 4052
DrdI GACnn_nn'nnGTC
Cuts at: 1758 2616 1758
Size: 858 3194
EaeI y'GGCC_r
Cuts at: 884 1637 1811 2202 2229 3574 884
Size: 753 174 391 27 1345 1362
Fragments arranged by size:
1362 1345 753 391 174 27
EagI C'GGCC_G
Cuts at: 1637 1637
Size: 4052
EarI CTCTTCn'nnn_
Cuts at: 2075 2285 2075
Size: 210 3842
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2260 2570 2716 3862 2260
Size: 310 146 1146 2450
Fragments arranged by size:
2450 1146 310 146
EcoICRI GAG'CTC
Cuts at: 1064 1064
Size: 4052
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 463 1877 2309 3056 3324 463
Size: 1414 432 747 268 1191
Fragments arranged by size:
1414 1191 747 432 268
EcoO109I rG'GnC_Cy
Cuts at: 1191 1191
Size: 4052
EcoRI G'AATT_C
Cuts at: 415 1056 415
Size: 641 3411
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2071 2071
Size: 4052
FspI TGC'GCA
Cuts at: 1833 1833
Size: 4052
HaeII r_GCGC'y
Cuts at: 1486 1734 2756 3735 1486
Size: 248 1022 979 1803
Fragments arranged by size:
1803 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1894 1924 1926 1956 1894
Size: 30 2 30 3990
Fragments arranged by size:
3990 30 30 2
HincII GTy'rAC
Cuts at: 1091 1091
Size: 4052
HindIII A'AGCT_T
Cuts at: 606 606
Size: 4052
KasI G'GCGC_C
Cuts at: 1482 1730 1482
Size: 248 3804
KpnI G_GTAC'C
Cuts at: 1072 1072
Size: 4052
MfeI C'AATT_G
Cuts at: 977 977
Size: 4052
MluI A'CGCG_T
Cuts at: 3485 3485
Size: 4052
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 2723 2907 2723
Size: 184 3868
MscI TGG'CCA
Cuts at: 1813 1813
Size: 4052
MslI CAynn'nnrTG
Cuts at: 659 754 845 2041 2168 659
Size: 95 91 1196 127 2543
Fragments arranged by size:
2543 1196 127 95 91
MspA1I CmG'CkG
Cuts at: 966 1163 1477 1837 2850 3095 966
Size: 197 314 360 1013 245 1923
Fragments arranged by size:
1923 1013 360 314 245 197
NaeI GCC'GGC
Cuts at: 2233 2233
Size: 4052
NarI GG'CG_CC
Cuts at: 1483 1731 1483
Size: 248 3804
NdeI CA'TA_TG
Cuts at: 129 129
Size: 4052
NgoMIV G'CCGG_C
Cuts at: 2231 2231
Size: 4052
NspI r_CATG'y
Cuts at: 216 573 913 1105 1605 2512 216
Size: 357 340 192 500 907 1756
Fragments arranged by size:
1756 907 500 357 340 192
PciI A'CATG_T
Cuts at: 2508 2508
Size: 4052
PmeI GTTT'AAAC
Cuts at: 1051 1051
Size: 4052
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3191 3223 3191
Size: 32 4020
PshAI GACnn'nnGTC
Cuts at: 526 526
Size: 4052
PspOMI G'GGCC_C
Cuts at: 283 283
Size: 4052
PstI C_TGCA'G
Cuts at: 211 1099 1784 211
Size: 888 685 2479
Fragments arranged by size:
2479 888 685
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4052
PvuII CAG'CTG
Cuts at: 1477 1837 1477
Size: 360 3692
RsrII CG'GwC_CG
Cuts at: 2247 2247
Size: 4052
SacI G_AGCT'C
Cuts at: 1066 1066
Size: 4052
SalI G'TCGA_C
Cuts at: 1089 1089
Size: 4052
SapI GCTCTTCn'nnn_
Cuts at: 2075 2285 2075
Size: 210 3842
SbfI CC_TGCA'GG
Cuts at: 1099 1099
Size: 4052
ScaI AGT'ACT
Cuts at: 440 3569 440
Size: 3129 923
SfcI C'TryA_G
Cuts at: 33 207 1095 1780 2773 2964 33
Size: 174 888 685 993 191 1121
Fragments arranged by size:
1121 993 888 685 191 174
SfoI GGC'GCC
Cuts at: 1484 1732 1484
Size: 248 3804
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4052
SmaI CCC'GGG
Cuts at: 1074 1074
Size: 4052
SmlI C'TyrA_G
Cuts at: 609 775 814 1207 2122 2614 2876 609
Size: 166 39 393 915 492 262 1785
Fragments arranged by size:
1785 915 492 393 262 166 39
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4052
SphI G_CATG'C
Cuts at: 573 1105 1605 573
Size: 532 500 3020
Fragments arranged by size:
3020 532 500
StyI C'CwwG_G
Cuts at: 1186 1540 1186
Size: 354 3698
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1635 1768 2227 2436 1635
Size: 133 459 209 3251
Fragments arranged by size:
3251 459 209 133
TatI w'GTAC_w
Cuts at: 290 438 3567 290
Size: 148 3129 775
Fragments arranged by size:
3129 775 148
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3569 3601 3569
Size: 32 4020
Tth111I GACn'n_nGTC
Cuts at: 1849 1849
Size: 4052
XbaI T'CTAG_A
Cuts at: 1083 1083
Size: 4052
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 150 879 1547 150
Size: 729 668 2655
Fragments arranged by size:
2655 729 668
XmaI C'CCGG_G
Cuts at: 1072 1072
Size: 4052
Enzymes that do cut:
AccI Acc65I AclI AcuI AflII AflIII AgeI AleI
AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI
BanI BanII BbeI BciVI BclI BglII BlpI Bme1580I
BmrI BplI BpmI BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BseRI BseYI BsgI BsiEI BsiHKAI Bsp1286I BspEI
BspHI BspMI BsrBI BsrDI BsrFI BssSI BstAPI BstBI
BstYI BtgZI BtsI DraI DrdI EaeI EagI EarI
EciI EcoICRI Eco57MI EcoO109I EcoRI FalI FspI HaeII
Hin4I HincII HindIII KasI KpnI MfeI MluI MmeI
MscI MslI MspA1I NaeI NarI NdeI NgoMIV NspI
PciI PmeI PpiI PshAI PspOMI PstI PvuI PvuII
RsrII SacI SalI SapI SbfI ScaI SfcI SfoI
SgfI SmaI SmlI SpeI SphI StyI TaqII TatI
TstI Tth111I XbaI XcmI XmaI
Enzymes that do not cut:
AarI AatII AfeI AhdI AlfI AloI AscI AvrII
BaeI BbsI BbvCI BcgI BfrBI BglI BmgBI BmtI
Bpu10I BsaXI BsiWI BsmI BsmBI BsrGI BssHII BstEII
BstXI BstZ17I Bsu36I BtgI ClaI CspCI DraIII EcoNI
EcoRV FseI FspAI HpaI NcoI NheI NotI NruI
NsiI PacI PasI PflMI PfoI PmlI PpuMI PsiI
PspXI PsrI SacII SanDI SexAI SfiI SgrAI SnaBI
SrfI SspI StuI SwaI XhoI XmnI ZraI