Restriction map for
pF1KE0230
(Circular) (Six-base) MAPSORT of: pF1KE0230.seq Check: 2993 from: 1 to: 3644
pF1KE0230 3644 bp
With 182 enzymes: SgfI *
April 24, 2009 13:12 ..
AccI GT'mk_AC
Cuts at: 682 682
Size: 3644
Acc65I G'GTAC_C
Cuts at: 660 660
Size: 3644
AclI AA'CG_TT
Cuts at: 3484 3484
Size: 3644
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1469 1901 2648 2916 1469
Size: 432 747 268 2197
Fragments arranged by size:
2197 747 432 268
AflIII A'CryG_T
Cuts at: 996 2100 3077 996
Size: 1104 977 1563
Fragments arranged by size:
1563 1104 977
AgeI A'CCGG_T
Cuts at: 2032 2032
Size: 3644
AlwNI CAG_nnn'CTG
Cuts at: 134 518 548 578 949 2516 134
Size: 384 30 30 371 1567 1262
Fragments arranged by size:
1567 1262 384 371 30 30
ApaLI G'TGCA_C
Cuts at: 2414 2414
Size: 3644
ApoI r'AATT_y
Cuts at: 648 2997 3253 648
Size: 2349 256 1039
Fragments arranged by size:
2349 1039 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 3644
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 3644
AvaI C'yCGr_G
Cuts at: 664 664
Size: 3644
BamHI G'GATC_C
Cuts at: 669 669
Size: 3644
BanI G'GyrC_C
Cuts at: 315 660 1074 1322 1357 315
Size: 345 414 248 35 2602
Fragments arranged by size:
2602 414 345 248 35
BanII G_rGCy'C
Cuts at: 358 388 658 1688 358
Size: 30 270 1030 2314
Fragments arranged by size:
2314 1030 270 30
BbeI G_GCGC'C
Cuts at: 1078 1326 1078
Size: 248 3396
BciVI GTATCCnnnnn_n'
Cuts at: 1542 2303 3644 1542
Size: 761 1341 1542
Fragments arranged by size:
1542 1341 761
BclI T'GATC_A
Cuts at: 1164 1164
Size: 3644
BglI GCCn_nnn'nGGC
Cuts at: 215 215
Size: 3644
BglII A'GATC_T
Cuts at: 1159 3196 1159
Size: 2037 1607
BlpI GC'TnA_GC
Cuts at: 756 756
Size: 3644
Bme1580I G_kGCm'C
Cuts at: 1269 1362 2418 1269
Size: 93 1056 2495
Fragments arranged by size:
2495 1056 93
BmrI ACTGGGnnnn_n'
Cuts at: 444 856 937 1272 3416 444
Size: 412 81 335 2144 672
Fragments arranged by size:
2144 672 412 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2011 2011
Size: 3644
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 111 147 231 252 111
Size: 36 84 21 3503
Fragments arranged by size:
3503 84 36 21
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 820 1699 2191 2489 820
Size: 879 492 298 1975
Fragments arranged by size:
1975 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 3348 3348
Size: 3644
BsaAI yAC'GTr
Cuts at: 997 997
Size: 3644
BsaBI GATnn'nnATC
Cuts at: 6 1158 3195 6
Size: 1152 2037 455
Fragments arranged by size:
2037 1152 455
BsaHI Gr'CG_yC
Cuts at: 1075 1323 2025 3521 1075
Size: 248 702 1496 1198
Fragments arranged by size:
1496 1198 702 248
BsaWI w'CCGG_w
Cuts at: 833 1354 2032 2306 2453 2864 833
Size: 521 678 274 147 411 1613
Fragments arranged by size:
1613 678 521 411 274 147
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 471 471
Size: 3644
BseYI C'CCAG_C
Cuts at: 1069 1827 2404 2900 1069
Size: 758 577 496 1813
Fragments arranged by size:
1813 758 577 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 315 315
Size: 3644
BsiEI CG_ry'CG
Cuts at: 104 210 1186 1232 2440 104
Size: 106 976 46 1208 1308
Fragments arranged by size:
1308 1208 976 106 46
BsiHKAI G_wGCw'C
Cuts at: 310 658 1436 1626 2418 310
Size: 348 778 190 792 1536
Fragments arranged by size:
1536 792 778 348 190
Bsp1286I G_dGCh'C
Cuts at: 310 358 388 658 1269 1362 1436 1626 1688
Size: 48 30 270 611 93 74 190 62
Cuts at: 1688 2418 310
Size: 730 1536
Fragments arranged by size:
1536 730 611 270 190 93 74 62
48 30
BspEI T'CCGG_A
Cuts at: 2864 2864
Size: 3644
BspHI T'CATG_A
Cuts at: 2820 2820
Size: 3644
BspMI ACCTGCnnnn'nnnn_
Cuts at: 694 908 1210 1591 694
Size: 214 302 381 2747
Fragments arranged by size:
2747 381 302 214
BsrBI CCG'CTC
Cuts at: 50 1936 1990 3474 3639 50
Size: 1886 54 1484 165 55
Fragments arranged by size:
1886 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 1556 1556
Size: 3644
BsrFI r'CCGG_y
Cuts at: 1642 1823 2032 1642
Size: 181 209 3254
Fragments arranged by size:
3254 209 181
BssSI C'ACGA_G
Cuts at: 1915 2273 1915
Size: 358 3286
BstAPI GCAn_nnn'nTGC
Cuts at: 527 1021 527
Size: 494 3150
BstBI TT'CG_AA
Cuts at: 2005 2005
Size: 3644
BstYI r'GATC_y
Cuts at: 1 669 1136 1159 1494 1740 2752 2838 2850
Size: 668 467 23 335 246 1012 86 12
Cuts at: 2850 3196 1
Size: 346 449
Fragments arranged by size:
1012 668 467 449 346 335 246 86
23 12
Bsu36I CC'TnA_GG
Cuts at: 483 483
Size: 3644
BtgI C'CryG_G
Cuts at: 107 210 107
Size: 103 3541
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 1594 1775 1938 1594
Size: 181 163 3300
Fragments arranged by size:
3300 181 163
BtsI GCAGTG_nn'
Cuts at: 887 887
Size: 3644
DraI TTT'AAA
Cuts at: 643 643
Size: 3644
DrdI GACnn_nn'nnGTC
Cuts at: 1350 2208 1350
Size: 858 2786
EaeI y'GGCC_r
Cuts at: 207 1229 1403 1794 1821 3166 207
Size: 1022 174 391 27 1345 685
Fragments arranged by size:
1345 1022 685 391 174 27
EagI C'GGCC_G
Cuts at: 207 1229 207
Size: 1022 2622
EarI CTCTTCn'nnn_
Cuts at: 1667 1877 1667
Size: 210 3434
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 1852 2162 2308 3454 1852
Size: 310 146 1146 2042
Fragments arranged by size:
2042 1146 310 146
EcoICRI GAG'CTC
Cuts at: 656 656
Size: 3644
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 111 147 231 252 1469 1901 2648 2916 111
Size: 36 84 21 1217 432 747 268 839
Fragments arranged by size:
1217 839 747 432 268 84 36 21
EcoO109I rG'GnC_Cy
Cuts at: 783 783
Size: 3644
EcoRI G'AATT_C
Cuts at: 648 648
Size: 3644
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 1663 1663
Size: 3644
FspI TGC'GCA
Cuts at: 1425 1425
Size: 3644
HaeII r_GCGC'y
Cuts at: 1078 1326 2348 3327 1078
Size: 248 1022 979 1395
Fragments arranged by size:
1395 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1486 1516 1518 1548 1486
Size: 30 2 30 3582
Fragments arranged by size:
3582 30 30 2
HincII GTy'rAC
Cuts at: 683 683
Size: 3644
KasI G'GCGC_C
Cuts at: 1074 1322 1074
Size: 248 3396
KpnI G_GTAC'C
Cuts at: 664 664
Size: 3644
MluI A'CGCG_T
Cuts at: 3077 3077
Size: 3644
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 626 2315 2499 626
Size: 1689 184 1771
Fragments arranged by size:
1771 1689 184
MscI TGG'CCA
Cuts at: 1405 1405
Size: 3644
MslI CAynn'nnrTG
Cuts at: 1633 1760 1633
Size: 127 3517
MspA1I CmG'CkG
Cuts at: 167 251 338 458 515 545 575 755 1069
Size: 84 87 120 57 30 30 180 314
Cuts at: 1069 1429 2442 2687 167
Size: 360 1013 245 1124
Fragments arranged by size:
1124 1013 360 314 245 180 120 87
84 57 30 30
NaeI GCC'GGC
Cuts at: 1825 1825
Size: 3644
NarI GG'CG_CC
Cuts at: 1075 1323 1075
Size: 248 3396
NcoI C'CATG_G
Cuts at: 107 107
Size: 3644
NgoMIV G'CCGG_C
Cuts at: 1823 1823
Size: 3644
NspI r_CATG'y
Cuts at: 697 1197 2104 697
Size: 500 907 2237
Fragments arranged by size:
2237 907 500
PciI A'CATG_T
Cuts at: 2100 2100
Size: 3644
PmeI GTTT'AAAC
Cuts at: 643 643
Size: 3644
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2783 2815 2783
Size: 32 3612
PstI C_TGCA'G
Cuts at: 244 691 1376 244
Size: 447 685 2512
Fragments arranged by size:
2512 685 447
PvuI CG_AT'CG
Cuts at: 104 104
Size: 3644
PvuII CAG'CTG
Cuts at: 167 251 338 458 515 545 575 1069 1429
Size: 84 87 120 57 30 30 494 360
Cuts at: 1429 167
Size: 2382
Fragments arranged by size:
2382 494 360 120 87 84 57 30
30
RsrII CG'GwC_CG
Cuts at: 1839 1839
Size: 3644
SacI G_AGCT'C
Cuts at: 658 658
Size: 3644
SalI G'TCGA_C
Cuts at: 681 681
Size: 3644
SapI GCTCTTCn'nnn_
Cuts at: 1667 1877 1667
Size: 210 3434
SbfI CC_TGCA'GG
Cuts at: 691 691
Size: 3644
ScaI AGT'ACT
Cuts at: 3161 3161
Size: 3644
SfcI C'TryA_G
Cuts at: 33 240 687 1372 2365 2556 33
Size: 207 447 685 993 191 1121
Fragments arranged by size:
1121 993 685 447 207 191
SfoI GGC'GCC
Cuts at: 1076 1324 1076
Size: 248 3396
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 3644
SmaI CCC'GGG
Cuts at: 666 666
Size: 3644
SmlI C'TyrA_G
Cuts at: 799 1714 2206 2468 799
Size: 915 492 262 1975
Fragments arranged by size:
1975 915 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 3644
SphI G_CATG'C
Cuts at: 697 1197 697
Size: 500 3144
StyI C'CwwG_G
Cuts at: 107 359 389 419 778 1132 107
Size: 252 30 30 359 354 2619
Fragments arranged by size:
2619 359 354 252 30 30
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 303 1227 1360 1819 2028 303
Size: 924 133 459 209 1919
Fragments arranged by size:
1919 924 459 209 133
TatI w'GTAC_w
Cuts at: 3159 3159
Size: 3644
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3161 3193 3161
Size: 32 3612
Tth111I GACn'n_nGTC
Cuts at: 1441 1441
Size: 3644
XbaI T'CTAG_A
Cuts at: 675 675
Size: 3644
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1139 1139
Size: 3644
XmaI C'CCGG_G
Cuts at: 664 664
Size: 3644
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AgeI AlwNI ApaLI
ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI
BciVI BclI BglI BglII BlpI Bme1580I BmrI BplI
BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BseRI
BseYI BsgI BsiEI BsiHKAI Bsp1286I BspEI BspHI BspMI
BsrBI BsrDI BsrFI BssSI BstAPI BstBI BstYI Bsu36I
BtgI BtgZI BtsI DraI DrdI EaeI EagI EarI
EciI EcoICRI Eco57MI EcoO109I EcoRI FalI FspI HaeII
Hin4I HincII KasI KpnI MluI MmeI MscI MslI
MspA1I NaeI NarI NcoI NgoMIV NspI PciI PmeI
PpiI PstI PvuI PvuII RsrII SacI SalI SapI
SbfI ScaI SfcI SfoI SgfI SmaI SmlI SpeI
SphI StyI TaqII TatI TstI Tth111I XbaI XcmI
XmaI
Enzymes that do not cut:
AarI AatII AfeI AflII AhdI AleI AlfI AloI
ApaI AscI AvrII BaeI BbsI BbvCI BcgI BfrBI
BmgBI BmtI Bpu10I BsaXI BsiWI BsmI BsmBI BsrGI
BssHII BstEII BstXI BstZ17I ClaI CspCI DraIII EcoNI
EcoRV FseI FspAI HindIII HpaI MfeI NdeI NheI
NotI NruI NsiI PacI PasI PflMI PfoI PmlI
PpuMI PshAI PsiI PspOMI PspXI PsrI SacII SanDI
SexAI SfiI SgrAI SnaBI SrfI SspI StuI SwaI
XhoI XmnI ZraI