Restriction map for
pF1KE0229
(Circular) (Six-base) MAPSORT of: pF1KE0229.seq Check: 6539 from: 1 to: 4025
pF1KE0229 4025 bp
With 182 enzymes: SgfI *
April 24, 2009 13:12 ..
AccI GT'mk_AC
Cuts at: 1063 1063
Size: 4025
Acc65I G'GTAC_C
Cuts at: 1041 1041
Size: 4025
AclI AA'CG_TT
Cuts at: 3865 3865
Size: 4025
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 778 1850 2282 3029 3297 778
Size: 1072 432 747 268 1506
Fragments arranged by size:
1506 1072 747 432 268
AflIII A'CryG_T
Cuts at: 1377 2481 3458 1377
Size: 1104 977 1944
Fragments arranged by size:
1944 1104 977
AgeI A'CCGG_T
Cuts at: 2413 2413
Size: 4025
AlwNI CAG_nnn'CTG
Cuts at: 1330 2897 1330
Size: 1567 2458
ApaLI G'TGCA_C
Cuts at: 2795 2795
Size: 4025
ApoI r'AATT_y
Cuts at: 299 385 604 616 788 958 1029 3378 3634
Size: 86 219 12 172 170 71 2349 256
Cuts at: 3634 299
Size: 690
Fragments arranged by size:
2349 690 256 219 172 170 86 71
12
AseI AT'TA_AT
Cuts at: 20 20
Size: 4025
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4025
AvaI C'yCGr_G
Cuts at: 1045 1045
Size: 4025
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 745 778 745
Size: 33 3992
BamHI G'GATC_C
Cuts at: 695 1050 695
Size: 355 3670
BanI G'GyrC_C
Cuts at: 1041 1455 1703 1738 1041
Size: 414 248 35 3328
Fragments arranged by size:
3328 414 248 35
BanII G_rGCy'C
Cuts at: 351 1039 2069 351
Size: 688 1030 2307
Fragments arranged by size:
2307 1030 688
BbeI G_GCGC'C
Cuts at: 1459 1707 1459
Size: 248 3777
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 999 1033 999
Size: 34 3991
BciVI GTATCCnnnnn_n'
Cuts at: 1923 2684 4025 1923
Size: 761 1341 1923
Fragments arranged by size:
1923 1341 761
BclI T'GATC_A
Cuts at: 1545 1545
Size: 4025
BfrBI ATG'CAT
Cuts at: 294 294
Size: 4025
BglII A'GATC_T
Cuts at: 1540 3577 1540
Size: 2037 1988
BlpI GC'TnA_GC
Cuts at: 1137 1137
Size: 4025
Bme1580I G_kGCm'C
Cuts at: 1650 1743 2799 1650
Size: 93 1056 2876
Fragments arranged by size:
2876 1056 93
BmrI ACTGGGnnnn_n'
Cuts at: 1237 1318 1653 3797 1237
Size: 81 335 2144 1465
Fragments arranged by size:
2144 1465 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2392 2392
Size: 4025
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 938 938
Size: 4025
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1201 2080 2572 2870 1201
Size: 879 492 298 2356
Fragments arranged by size:
2356 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 3729 3729
Size: 4025
BsaAI yAC'GTr
Cuts at: 1378 1378
Size: 4025
BsaBI GATnn'nnATC
Cuts at: 6 1539 3576 6
Size: 1533 2037 455
Fragments arranged by size:
2037 1533 455
BsaHI Gr'CG_yC
Cuts at: 1456 1704 2406 3902 1456
Size: 248 702 1496 1579
Fragments arranged by size:
1579 1496 702 248
BsaWI w'CCGG_w
Cuts at: 625 692 1214 1735 2413 2687 2834 3245 625
Size: 67 522 521 678 274 147 411 1405
Fragments arranged by size:
1405 678 522 521 411 274 147 67
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 874 904 874
Size: 30 3995
BseYI C'CCAG_C
Cuts at: 1450 2208 2785 3281 1450
Size: 758 577 496 2194
Fragments arranged by size:
2194 758 577 496
BsiEI CG_ry'CG
Cuts at: 104 1567 1613 2821 104
Size: 1463 46 1208 1308
Fragments arranged by size:
1463 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 1039 1817 2007 2799 1039
Size: 778 190 792 2265
Fragments arranged by size:
2265 792 778 190
Bsp1286I G_dGCh'C
Cuts at: 351 1039 1650 1743 1817 2007 2069 2799 351
Size: 688 611 93 74 190 62 730 1577
Fragments arranged by size:
1577 730 688 611 190 93 74 62
BspEI T'CCGG_A
Cuts at: 625 3245 625
Size: 2620 1405
BspHI T'CATG_A
Cuts at: 3201 3201
Size: 4025
BspMI ACCTGCnnnn'nnnn_
Cuts at: 1075 1289 1591 1972 1075
Size: 214 302 381 3128
Fragments arranged by size:
3128 381 302 214
BsrBI CCG'CTC
Cuts at: 50 2317 2371 3855 4020 50
Size: 2267 54 1484 165 55
Fragments arranged by size:
2267 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 1937 1937
Size: 4025
BsrFI r'CCGG_y
Cuts at: 2023 2204 2413 2023
Size: 181 209 3635
Fragments arranged by size:
3635 209 181
BssSI C'ACGA_G
Cuts at: 2296 2654 2296
Size: 358 3667
BstAPI GCAn_nnn'nTGC
Cuts at: 1402 1402
Size: 4025
BstBI TT'CG_AA
Cuts at: 2386 2386
Size: 4025
BstXI CCAn_nnnn'nTGG
Cuts at: 488 876 488
Size: 388 3637
BstYI r'GATC_y
Cuts at: 1 695 1050 1517 1540 1875 2121 3133 3219
Size: 694 355 467 23 335 246 1012 86
Cuts at: 3219 3231 3577 1
Size: 12 346 449
Fragments arranged by size:
1012 694 467 449 355 346 335 246
86 23 12
BtgI C'CryG_G
Cuts at: 107 107
Size: 4025
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 1975 2156 2319 1975
Size: 181 163 3681
Fragments arranged by size:
3681 181 163
BtsI GCAGTG_nn'
Cuts at: 1268 1268
Size: 4025
DraI TTT'AAA
Cuts at: 1024 1024
Size: 4025
DrdI GACnn_nn'nnGTC
Cuts at: 1731 2589 1731
Size: 858 3167
EaeI y'GGCC_r
Cuts at: 518 1610 1784 2175 2202 3547 518
Size: 1092 174 391 27 1345 996
Fragments arranged by size:
1345 1092 996 391 174 27
EagI C'GGCC_G
Cuts at: 1610 1610
Size: 4025
EarI CTCTTCn'nnn_
Cuts at: 712 2048 2258 712
Size: 1336 210 2479
Fragments arranged by size:
2479 1336 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2233 2543 2689 3835 2233
Size: 310 146 1146 2423
Fragments arranged by size:
2423 1146 310 146
EcoICRI GAG'CTC
Cuts at: 1037 1037
Size: 4025
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 778 938 1850 2282 3029 3297 778
Size: 160 912 432 747 268 1506
Fragments arranged by size:
1506 912 747 432 268 160
EcoO109I rG'GnC_Cy
Cuts at: 644 1164 644
Size: 520 3505
EcoRI G'AATT_C
Cuts at: 1029 1029
Size: 4025
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2044 2044
Size: 4025
FspI TGC'GCA
Cuts at: 1806 1806
Size: 4025
HaeII r_GCGC'y
Cuts at: 1459 1707 2729 3708 1459
Size: 248 1022 979 1776
Fragments arranged by size:
1776 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1867 1897 1899 1929 1867
Size: 30 2 30 3963
Fragments arranged by size:
3963 30 30 2
HincII GTy'rAC
Cuts at: 1064 1064
Size: 4025
KasI G'GCGC_C
Cuts at: 1455 1703 1455
Size: 248 3777
KpnI G_GTAC'C
Cuts at: 1045 1045
Size: 4025
MluI A'CGCG_T
Cuts at: 3458 3458
Size: 4025
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 2696 2880 2696
Size: 184 3841
MscI TGG'CCA
Cuts at: 520 1786 520
Size: 1266 2759
MslI CAynn'nnrTG
Cuts at: 2014 2141 2014
Size: 127 3898
MspA1I CmG'CkG
Cuts at: 1136 1450 1810 2823 3068 1136
Size: 314 360 1013 245 2093
Fragments arranged by size:
2093 1013 360 314 245
NaeI GCC'GGC
Cuts at: 2206 2206
Size: 4025
NarI GG'CG_CC
Cuts at: 1456 1704 1456
Size: 248 3777
NcoI C'CATG_G
Cuts at: 107 107
Size: 4025
NgoMIV G'CCGG_C
Cuts at: 2204 2204
Size: 4025
NsiI A_TGCA'T
Cuts at: 296 296
Size: 4025
NspI r_CATG'y
Cuts at: 690 1078 1578 2485 690
Size: 388 500 907 2230
Fragments arranged by size:
2230 907 500 388
PciI A'CATG_T
Cuts at: 2481 2481
Size: 4025
PfoI T'CCnGG_A
Cuts at: 764 764
Size: 4025
PmeI GTTT'AAAC
Cuts at: 1024 1024
Size: 4025
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3164 3196 3164
Size: 32 3993
PpuMI rG'GwC_Cy
Cuts at: 644 644
Size: 4025
PstI C_TGCA'G
Cuts at: 1072 1757 1072
Size: 685 3340
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4025
PvuII CAG'CTG
Cuts at: 1450 1810 1450
Size: 360 3665
RsrII CG'GwC_CG
Cuts at: 2220 2220
Size: 4025
SacI G_AGCT'C
Cuts at: 1039 1039
Size: 4025
SalI G'TCGA_C
Cuts at: 1062 1062
Size: 4025
SapI GCTCTTCn'nnn_
Cuts at: 2048 2258 2048
Size: 210 3815
SbfI CC_TGCA'GG
Cuts at: 1072 1072
Size: 4025
ScaI AGT'ACT
Cuts at: 3542 3542
Size: 4025
SfcI C'TryA_G
Cuts at: 33 1068 1753 2746 2937 33
Size: 1035 685 993 191 1121
Fragments arranged by size:
1121 1035 993 685 191
SfoI GGC'GCC
Cuts at: 1457 1705 1457
Size: 248 3777
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4025
SmaI CCC'GGG
Cuts at: 1047 1047
Size: 4025
SmlI C'TyrA_G
Cuts at: 1180 2095 2587 2849 1180
Size: 915 492 262 2356
Fragments arranged by size:
2356 915 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4025
SphI G_CATG'C
Cuts at: 1078 1578 1078
Size: 500 3525
StyI C'CwwG_G
Cuts at: 107 530 1159 1513 107
Size: 423 629 354 2619
Fragments arranged by size:
2619 629 423 354
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1608 1741 2200 2409 1608
Size: 133 459 209 3224
Fragments arranged by size:
3224 459 209 133
TatI w'GTAC_w
Cuts at: 3540 3540
Size: 4025
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 874 906 3542 3574 874
Size: 32 2636 32 1325
Fragments arranged by size:
2636 1325 32 32
Tth111I GACn'n_nGTC
Cuts at: 1822 1822
Size: 4025
XbaI T'CTAG_A
Cuts at: 357 1056 357
Size: 699 3326
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 485 537 873 1520 485
Size: 52 336 647 2990
Fragments arranged by size:
2990 647 336 52
XmaI C'CCGG_G
Cuts at: 1045 1045
Size: 4025
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AgeI AlwNI ApaLI
ApoI AseI AsiSI AvaI BaeI BamHI BanI BanII
BbeI BcgI BciVI BclI BfrBI BglII BlpI Bme1580I
BmrI BplI BpmI BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseYI BsiEI BsiHKAI Bsp1286I BspEI BspHI
BspMI BsrBI BsrDI BsrFI BssSI BstAPI BstBI BstXI
BstYI BtgI BtgZI BtsI DraI DrdI EaeI EagI
EarI EciI EcoICRI Eco57MI EcoO109I EcoRI FalI FspI
HaeII Hin4I HincII KasI KpnI MluI MmeI MscI
MslI MspA1I NaeI NarI NcoI NgoMIV NsiI NspI
PciI PfoI PmeI PpiI PpuMI PstI PvuI PvuII
RsrII SacI SalI SapI SbfI ScaI SfcI SfoI
SgfI SmaI SmlI SpeI SphI StyI TaqII TatI
TstI Tth111I XbaI XcmI XmaI
Enzymes that do not cut:
AarI AatII AfeI AflII AhdI AleI AlfI AloI
ApaI AscI AvrII BbsI BbvCI BglI BmgBI BmtI
Bpu10I BseRI BsgI BsiWI BsmI BsmBI BsrGI BssHII
BstEII BstZ17I Bsu36I ClaI CspCI DraIII EcoNI EcoRV
FseI FspAI HindIII HpaI MfeI NdeI NheI NotI
NruI PacI PasI PflMI PmlI PshAI PsiI PspOMI
PspXI PsrI SacII SanDI SexAI SfiI SgrAI SnaBI
SrfI SspI StuI SwaI XhoI XmnI ZraI