Restriction map for
pF1KE0227
(Circular) (Six-base) MAPSORT of: pF1KE0227.seq Check: 9986 from: 1 to: 3443
pF1KE0227 3443 bp
With 182 enzymes: SgfI *
April 24, 2009 13:12 ..
AccI GT'mk_AC
Cuts at: 481 481
Size: 3443
Acc65I G'GTAC_C
Cuts at: 459 459
Size: 3443
AclI AA'CG_TT
Cuts at: 3283 3283
Size: 3443
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1268 1700 2447 2715 1268
Size: 432 747 268 1996
Fragments arranged by size:
1996 747 432 268
AflIII A'CryG_T
Cuts at: 795 1899 2876 795
Size: 1104 977 1362
Fragments arranged by size:
1362 1104 977
AgeI A'CCGG_T
Cuts at: 1831 1831
Size: 3443
AleI CACnn'nnGTG
Cuts at: 186 186
Size: 3443
AlwNI CAG_nnn'CTG
Cuts at: 748 2315 748
Size: 1567 1876
ApaLI G'TGCA_C
Cuts at: 2213 2213
Size: 3443
ApoI r'AATT_y
Cuts at: 447 2796 3052 447
Size: 2349 256 838
Fragments arranged by size:
2349 838 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 3443
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 3443
AvaI C'yCGr_G
Cuts at: 463 463
Size: 3443
BamHI G'GATC_C
Cuts at: 468 468
Size: 3443
BanI G'GyrC_C
Cuts at: 356 362 459 873 1121 1156 356
Size: 6 97 414 248 35 2643
Fragments arranged by size:
2643 414 248 97 35 6
BanII G_rGCy'C
Cuts at: 373 406 457 1487 373
Size: 33 51 1030 2329
Fragments arranged by size:
2329 1030 51 33
BbeI G_GCGC'C
Cuts at: 360 877 1125 360
Size: 517 248 2678
Fragments arranged by size:
2678 517 248
BciVI GTATCCnnnnn_n'
Cuts at: 1341 2102 3443 1341
Size: 761 1341 1341
Fragments arranged by size:
1341 1341 761
BclI T'GATC_A
Cuts at: 963 963
Size: 3443
BglII A'GATC_T
Cuts at: 958 2995 958
Size: 2037 1406
BlpI GC'TnA_GC
Cuts at: 555 555
Size: 3443
Bme1580I G_kGCm'C
Cuts at: 217 1068 1161 2217 217
Size: 851 93 1056 1443
Fragments arranged by size:
1443 1056 851 93
BmrI ACTGGGnnnn_n'
Cuts at: 204 655 736 1071 3215 204
Size: 451 81 335 2144 432
Fragments arranged by size:
2144 451 432 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 1810 1810
Size: 3443
Bpu10I CC'TnA_GC
Cuts at: 325 325
Size: 3443
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 619 1498 1990 2288 619
Size: 879 492 298 1774
Fragments arranged by size:
1774 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 3147 3147
Size: 3443
BsaAI yAC'GTr
Cuts at: 796 796
Size: 3443
BsaBI GATnn'nnATC
Cuts at: 6 957 2994 6
Size: 951 2037 455
Fragments arranged by size:
2037 951 455
BsaHI Gr'CG_yC
Cuts at: 357 874 1122 1824 3320 357
Size: 517 248 702 1496 480
Fragments arranged by size:
1496 702 517 480 248
BsaWI w'CCGG_w
Cuts at: 632 1153 1831 2105 2252 2663 632
Size: 521 678 274 147 411 1412
Fragments arranged by size:
1412 678 521 411 274 147
BseYI C'CCAG_C
Cuts at: 216 280 868 1626 2203 2699 216
Size: 64 588 758 577 496 960
Fragments arranged by size:
960 758 588 577 496 64
BsiEI CG_ry'CG
Cuts at: 104 985 1031 2239 104
Size: 881 46 1208 1308
Fragments arranged by size:
1308 1208 881 46
BsiHKAI G_wGCw'C
Cuts at: 457 1235 1425 2217 457
Size: 778 190 792 1683
Fragments arranged by size:
1683 792 778 190
BsmBI CGTCTCn'nnnn_
Cuts at: 349 349
Size: 3443
Bsp1286I G_dGCh'C
Cuts at: 217 373 406 457 1068 1161 1235 1425 1487
Size: 156 33 51 611 93 74 190 62
Cuts at: 1487 2217 217
Size: 730 1443
Fragments arranged by size:
1443 730 611 190 156 93 74 62
51 33
BspEI T'CCGG_A
Cuts at: 2663 2663
Size: 3443
BspHI T'CATG_A
Cuts at: 2619 2619
Size: 3443
BspMI ACCTGCnnnn'nnnn_
Cuts at: 493 707 1009 1390 493
Size: 214 302 381 2546
Fragments arranged by size:
2546 381 302 214
BsrBI CCG'CTC
Cuts at: 50 1735 1789 3273 3438 50
Size: 1685 54 1484 165 55
Fragments arranged by size:
1685 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 1355 1355
Size: 3443
BsrFI r'CCGG_y
Cuts at: 353 359 1441 1622 1831 353
Size: 6 1082 181 209 1965
Fragments arranged by size:
1965 1082 209 181 6
BssSI C'ACGA_G
Cuts at: 1714 2072 1714
Size: 358 3085
BstAPI GCAn_nnn'nTGC
Cuts at: 820 820
Size: 3443
BstBI TT'CG_AA
Cuts at: 1804 1804
Size: 3443
BstXI CCAn_nnnn'nTGG
Cuts at: 424 424
Size: 3443
BstYI r'GATC_y
Cuts at: 1 468 935 958 1293 1539 2551 2637 2649
Size: 467 467 23 335 246 1012 86 12
Cuts at: 2649 2995 1
Size: 346 449
Fragments arranged by size:
1012 467 467 449 346 335 246 86
23 12
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 1393 1574 1737 1393
Size: 181 163 3099
Fragments arranged by size:
3099 181 163
BtsI GCAGTG_nn'
Cuts at: 139 686 139
Size: 547 2896
DraI TTT'AAA
Cuts at: 442 442
Size: 3443
DrdI GACnn_nn'nnGTC
Cuts at: 1149 2007 1149
Size: 858 2585
EaeI y'GGCC_r
Cuts at: 1028 1202 1593 1620 2965 1028
Size: 174 391 27 1345 1506
Fragments arranged by size:
1506 1345 391 174 27
EagI C'GGCC_G
Cuts at: 1028 1028
Size: 3443
EarI CTCTTCn'nnn_
Cuts at: 246 352 1466 1676 246
Size: 106 1114 210 2013
Fragments arranged by size:
2013 1114 210 106
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 1651 1961 2107 3253 1651
Size: 310 146 1146 1841
Fragments arranged by size:
1841 1146 310 146
EcoICRI GAG'CTC
Cuts at: 455 455
Size: 3443
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1268 1700 2447 2715 1268
Size: 432 747 268 1996
Fragments arranged by size:
1996 747 432 268
EcoO109I rG'GnC_Cy
Cuts at: 338 582 338
Size: 244 3199
EcoRI G'AATT_C
Cuts at: 447 447
Size: 3443
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 1462 1462
Size: 3443
FspI TGC'GCA
Cuts at: 1224 1224
Size: 3443
HaeII r_GCGC'y
Cuts at: 360 877 1125 2147 3126 360
Size: 517 248 1022 979 677
Fragments arranged by size:
1022 979 677 517 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1285 1315 1317 1347 1285
Size: 30 2 30 3381
Fragments arranged by size:
3381 30 30 2
HincII GTy'rAC
Cuts at: 482 482
Size: 3443
KasI G'GCGC_C
Cuts at: 356 873 1121 356
Size: 517 248 2678
Fragments arranged by size:
2678 517 248
KpnI G_GTAC'C
Cuts at: 463 463
Size: 3443
MluI A'CGCG_T
Cuts at: 2876 2876
Size: 3443
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 2114 2298 2114
Size: 184 3259
MscI TGG'CCA
Cuts at: 1204 1204
Size: 3443
MslI CAynn'nnrTG
Cuts at: 186 1432 1559 186
Size: 1246 127 2070
Fragments arranged by size:
2070 1246 127
MspA1I CmG'CkG
Cuts at: 554 868 1228 2241 2486 554
Size: 314 360 1013 245 1511
Fragments arranged by size:
1511 1013 360 314 245
NaeI GCC'GGC
Cuts at: 361 1624 361
Size: 1263 2180
NarI GG'CG_CC
Cuts at: 357 874 1122 357
Size: 517 248 2678
Fragments arranged by size:
2678 517 248
NgoMIV G'CCGG_C
Cuts at: 359 1622 359
Size: 1263 2180
NspI r_CATG'y
Cuts at: 496 996 1903 496
Size: 500 907 2036
Fragments arranged by size:
2036 907 500
PciI A'CATG_T
Cuts at: 1899 1899
Size: 3443
PmeI GTTT'AAAC
Cuts at: 442 442
Size: 3443
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2582 2614 2582
Size: 32 3411
PstI C_TGCA'G
Cuts at: 237 490 1175 237
Size: 253 685 2505
Fragments arranged by size:
2505 685 253
PvuI CG_AT'CG
Cuts at: 104 104
Size: 3443
PvuII CAG'CTG
Cuts at: 868 1228 868
Size: 360 3083
RsrII CG'GwC_CG
Cuts at: 1638 1638
Size: 3443
SacI G_AGCT'C
Cuts at: 457 457
Size: 3443
SalI G'TCGA_C
Cuts at: 480 480
Size: 3443
SapI GCTCTTCn'nnn_
Cuts at: 1466 1676 1466
Size: 210 3233
SbfI CC_TGCA'GG
Cuts at: 490 490
Size: 3443
ScaI AGT'ACT
Cuts at: 2960 2960
Size: 3443
SfcI C'TryA_G
Cuts at: 33 233 486 1171 2164 2355 33
Size: 200 253 685 993 191 1121
Fragments arranged by size:
1121 993 685 253 200 191
SfoI GGC'GCC
Cuts at: 358 875 1123 358
Size: 517 248 2678
Fragments arranged by size:
2678 517 248
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 3443
SgrAI Cr'CCGG_yG
Cuts at: 353 353
Size: 3443
SmaI CCC'GGG
Cuts at: 465 465
Size: 3443
SmlI C'TyrA_G
Cuts at: 598 1513 2005 2267 598
Size: 915 492 262 1774
Fragments arranged by size:
1774 915 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 3443
SphI G_CATG'C
Cuts at: 496 996 496
Size: 500 2943
StyI C'CwwG_G
Cuts at: 577 931 577
Size: 354 3089
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1026 1159 1618 1827 1026
Size: 133 459 209 2642
Fragments arranged by size:
2642 459 209 133
TatI w'GTAC_w
Cuts at: 2958 2958
Size: 3443
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2960 2992 2960
Size: 32 3411
Tth111I GACn'n_nGTC
Cuts at: 1240 1240
Size: 3443
XbaI T'CTAG_A
Cuts at: 474 474
Size: 3443
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 938 938
Size: 3443
XmaI C'CCGG_G
Cuts at: 463 463
Size: 3443
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AgeI AleI AlwNI
ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII
BbeI BciVI BclI BglII BlpI Bme1580I BmrI BplI
Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BseYI
BsiEI BsiHKAI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI
BsrDI BsrFI BssSI BstAPI BstBI BstXI BstYI BtgZI
BtsI DraI DrdI EaeI EagI EarI EciI EcoICRI
Eco57MI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII
KasI KpnI MluI MmeI MscI MslI MspA1I NaeI
NarI NgoMIV NspI PciI PmeI PpiI PstI PvuI
PvuII RsrII SacI SalI SapI SbfI ScaI SfcI
SfoI SgfI SgrAI SmaI SmlI SpeI SphI StyI
TaqII TatI TstI Tth111I XbaI XcmI XmaI
Enzymes that do not cut:
AarI AatII AfeI AflII AhdI AlfI AloI ApaI
AscI AvrII BaeI BbsI BbvCI BcgI BfrBI BglI
BmgBI BmtI BpmI BsaXI BseRI BsgI BsiWI BsmI
BsrGI BssHII BstEII BstZ17I Bsu36I BtgI ClaI CspCI
DraIII EcoNI EcoRV FseI FspAI HindIII HpaI MfeI
NcoI NdeI NheI NotI NruI NsiI PacI PasI
PflMI PfoI PmlI PpuMI PshAI PsiI PspOMI PspXI
PsrI SacII SanDI SexAI SfiI SnaBI SrfI SspI
StuI SwaI XhoI XmnI ZraI