Restriction map for
pF1KE0216
(Circular) (Six-base) MAPSORT of: pF1KE0216.seq Check: 5549 from: 1 to: 3695
pF1KE0216 3695 bp
With 182 enzymes: SgfI *
April 24, 2009 13:11 ..
AccI GT'mk_AC
Cuts at: 733 733
Size: 3695
Acc65I G'GTAC_C
Cuts at: 711 711
Size: 3695
AclI AA'CG_TT
Cuts at: 3535 3535
Size: 3695
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1520 1952 2699 2967 1520
Size: 432 747 268 2248
Fragments arranged by size:
2248 747 432 268
AflIII A'CryG_T
Cuts at: 341 1047 2151 3128 341
Size: 706 1104 977 908
Fragments arranged by size:
1104 977 908 706
AgeI A'CCGG_T
Cuts at: 2083 2083
Size: 3695
AlwNI CAG_nnn'CTG
Cuts at: 575 1000 2567 575
Size: 425 1567 1703
Fragments arranged by size:
1703 1567 425
ApaI G_GGCC'C
Cuts at: 300 300
Size: 3695
ApaLI G'TGCA_C
Cuts at: 2465 2465
Size: 3695
ApoI r'AATT_y
Cuts at: 699 3048 3304 699
Size: 2349 256 1090
Fragments arranged by size:
2349 1090 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 3695
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 3695
AvaI C'yCGr_G
Cuts at: 511 715 511
Size: 204 3491
BamHI G'GATC_C
Cuts at: 720 720
Size: 3695
BanI G'GyrC_C
Cuts at: 159 711 1125 1373 1408 159
Size: 552 414 248 35 2446
Fragments arranged by size:
2446 552 414 248 35
BanII G_rGCy'C
Cuts at: 300 638 709 1739 300
Size: 338 71 1030 2256
Fragments arranged by size:
2256 1030 338 71
BbeI G_GCGC'C
Cuts at: 163 1129 1377 163
Size: 966 248 2481
Fragments arranged by size:
2481 966 248
BciVI GTATCCnnnnn_n'
Cuts at: 1593 2354 3695 1593
Size: 761 1341 1593
Fragments arranged by size:
1593 1341 761
BclI T'GATC_A
Cuts at: 1215 1215
Size: 3695
BglI GCCn_nnn'nGGC
Cuts at: 407 407
Size: 3695
BglII A'GATC_T
Cuts at: 1210 3247 1210
Size: 2037 1658
BlpI GC'TnA_GC
Cuts at: 807 807
Size: 3695
Bme1580I G_kGCm'C
Cuts at: 300 1320 1413 2469 300
Size: 1020 93 1056 1526
Fragments arranged by size:
1526 1056 1020 93
BmgBI CAC'GTC
Cuts at: 198 279 342 198
Size: 81 63 3551
Fragments arranged by size:
3551 81 63
BmrI ACTGGGnnnn_n'
Cuts at: 907 988 1323 3467 907
Size: 81 335 2144 1135
Fragments arranged by size:
2144 1135 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 330 2062 330
Size: 1732 1963
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 145 187 552 691 145
Size: 42 365 139 3149
Fragments arranged by size:
3149 365 139 42
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 871 1750 2242 2540 871
Size: 879 492 298 2026
Fragments arranged by size:
2026 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 610 3399 610
Size: 2789 906
BsaAI yAC'GTr
Cuts at: 1048 1048
Size: 3695
BsaBI GATnn'nnATC
Cuts at: 6 1209 3246 6
Size: 1203 2037 455
Fragments arranged by size:
2037 1203 455
BsaHI Gr'CG_yC
Cuts at: 160 1126 1374 2076 3572 160
Size: 966 248 702 1496 283
Fragments arranged by size:
1496 966 702 283 248
BsaWI w'CCGG_w
Cuts at: 884 1405 2083 2357 2504 2915 884
Size: 521 678 274 147 411 1664
Fragments arranged by size:
1664 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 187 217 187
Size: 30 3665
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 535 535
Size: 3695
BseYI C'CCAG_C
Cuts at: 254 498 550 1120 1878 2455 2951 254
Size: 244 52 570 758 577 496 998
Fragments arranged by size:
998 758 577 570 496 244 52
BsiEI CG_ry'CG
Cuts at: 104 1237 1283 2491 104
Size: 1133 46 1208 1308
Fragments arranged by size:
1308 1208 1133 46
BsiHKAI G_wGCw'C
Cuts at: 245 332 709 1487 1677 2469 245
Size: 87 377 778 190 792 1471
Fragments arranged by size:
1471 792 778 377 190 87
BsmBI CGTCTCn'nnnn_
Cuts at: 372 372
Size: 3695
Bsp1286I G_dGCh'C
Cuts at: 245 300 332 638 709 1320 1413 1487 1677
Size: 55 32 306 71 611 93 74 190
Cuts at: 1677 1739 2469 245
Size: 62 730 1471
Fragments arranged by size:
1471 730 611 306 190 93 74 71
62 55 32
BspEI T'CCGG_A
Cuts at: 2915 2915
Size: 3695
BspHI T'CATG_A
Cuts at: 131 2871 131
Size: 2740 955
BspMI ACCTGCnnnn'nnnn_
Cuts at: 745 959 1261 1642 745
Size: 214 302 381 2798
Fragments arranged by size:
2798 381 302 214
BsrBI CCG'CTC
Cuts at: 50 1987 2041 3525 3690 50
Size: 1937 54 1484 165 55
Fragments arranged by size:
1937 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 1607 1607
Size: 3695
BsrFI r'CCGG_y
Cuts at: 156 183 1693 1874 2083 156
Size: 27 1510 181 209 1768
Fragments arranged by size:
1768 1510 209 181 27
BssSI C'ACGA_G
Cuts at: 1966 2324 1966
Size: 358 3337
BstAPI GCAn_nnn'nTGC
Cuts at: 1072 1072
Size: 3695
BstBI TT'CG_AA
Cuts at: 2056 2056
Size: 3695
BstYI r'GATC_y
Cuts at: 1 720 1187 1210 1545 1791 2803 2889 2901
Size: 719 467 23 335 246 1012 86 12
Cuts at: 2901 3247 1
Size: 346 449
Fragments arranged by size:
1012 719 467 449 346 335 246 86
23 12
BtgI C'CryG_G
Cuts at: 107 107
Size: 3695
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 1645 1826 1989 1645
Size: 181 163 3351
Fragments arranged by size:
3351 181 163
BtsI GCAGTG_nn'
Cuts at: 194 389 938 194
Size: 195 549 2951
Fragments arranged by size:
2951 549 195
DraI TTT'AAA
Cuts at: 694 694
Size: 3695
DrdI GACnn_nn'nnGTC
Cuts at: 1401 2259 1401
Size: 858 2837
EaeI y'GGCC_r
Cuts at: 1280 1454 1845 1872 3217 1280
Size: 174 391 27 1345 1758
Fragments arranged by size:
1758 1345 391 174 27
EagI C'GGCC_G
Cuts at: 1280 1280
Size: 3695
EarI CTCTTCn'nnn_
Cuts at: 530 1718 1928 530
Size: 1188 210 2297
Fragments arranged by size:
2297 1188 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 1903 2213 2359 3505 1903
Size: 310 146 1146 2093
Fragments arranged by size:
2093 1146 310 146
EcoICRI GAG'CTC
Cuts at: 707 707
Size: 3695
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 145 187 552 691 1520 1952 2699 2967 145
Size: 42 365 139 829 432 747 268 873
Fragments arranged by size:
873 829 747 432 365 268 139 42
EcoNI CCTnn'n_nnAGG
Cuts at: 271 271
Size: 3695
EcoO109I rG'GnC_Cy
Cuts at: 296 297 834 296
Size: 1 537 3157
Fragments arranged by size:
3157 537 1
EcoRI G'AATT_C
Cuts at: 699 699
Size: 3695
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 453 1714 453
Size: 1261 2434
FspI TGC'GCA
Cuts at: 1476 1476
Size: 3695
HaeII r_GCGC'y
Cuts at: 163 585 1129 1377 2399 3378 163
Size: 422 544 248 1022 979 480
Fragments arranged by size:
1022 979 544 480 422 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 187 219 350 382 1537 1567 1569 1599 187
Size: 32 131 32 1155 30 2 30 2283
Fragments arranged by size:
2283 1155 131 32 32 30 30 2
HincII GTy'rAC
Cuts at: 734 734
Size: 3695
KasI G'GCGC_C
Cuts at: 159 1125 1373 159
Size: 966 248 2481
Fragments arranged by size:
2481 966 248
KpnI G_GTAC'C
Cuts at: 715 715
Size: 3695
MluI A'CGCG_T
Cuts at: 3128 3128
Size: 3695
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 2366 2550 2366
Size: 184 3511
MscI TGG'CCA
Cuts at: 1456 1456
Size: 3695
MslI CAynn'nnrTG
Cuts at: 325 1684 1811 325
Size: 1359 127 2209
Fragments arranged by size:
2209 1359 127
MspA1I CmG'CkG
Cuts at: 258 491 806 1120 1480 2493 2738 258
Size: 233 315 314 360 1013 245 1215
Fragments arranged by size:
1215 1013 360 315 314 245 233
NaeI GCC'GGC
Cuts at: 158 185 1876 158
Size: 27 1691 1977
Fragments arranged by size:
1977 1691 27
NarI GG'CG_CC
Cuts at: 160 1126 1374 160
Size: 966 248 2481
Fragments arranged by size:
2481 966 248
NcoI C'CATG_G
Cuts at: 107 107
Size: 3695
NgoMIV G'CCGG_C
Cuts at: 156 183 1874 156
Size: 27 1691 1977
Fragments arranged by size:
1977 1691 27
NruI TCG'CGA
Cuts at: 506 506
Size: 3695
NspI r_CATG'y
Cuts at: 156 748 1248 2155 156
Size: 592 500 907 1696
Fragments arranged by size:
1696 907 592 500
PciI A'CATG_T
Cuts at: 2151 2151
Size: 3695
PmeI GTTT'AAAC
Cuts at: 694 694
Size: 3695
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2834 2866 2834
Size: 32 3663
PspOMI G'GGCC_C
Cuts at: 296 296
Size: 3695
PstI C_TGCA'G
Cuts at: 742 1427 742
Size: 685 3010
PvuI CG_AT'CG
Cuts at: 104 104
Size: 3695
PvuII CAG'CTG
Cuts at: 258 1120 1480 258
Size: 862 360 2473
Fragments arranged by size:
2473 862 360
RsrII CG'GwC_CG
Cuts at: 1890 1890
Size: 3695
SacI G_AGCT'C
Cuts at: 709 709
Size: 3695
SalI G'TCGA_C
Cuts at: 732 732
Size: 3695
SapI GCTCTTCn'nnn_
Cuts at: 1718 1928 1718
Size: 210 3485
SbfI CC_TGCA'GG
Cuts at: 742 742
Size: 3695
ScaI AGT'ACT
Cuts at: 3212 3212
Size: 3695
SfcI C'TryA_G
Cuts at: 33 738 1423 2416 2607 33
Size: 705 685 993 191 1121
Fragments arranged by size:
1121 993 705 685 191
SfoI GGC'GCC
Cuts at: 161 1127 1375 161
Size: 966 248 2481
Fragments arranged by size:
2481 966 248
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 3695
SmaI CCC'GGG
Cuts at: 717 717
Size: 3695
SmlI C'TyrA_G
Cuts at: 850 1765 2257 2519 850
Size: 915 492 262 2026
Fragments arranged by size:
2026 915 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 3695
SphI G_CATG'C
Cuts at: 748 1248 748
Size: 500 3195
StuI AGG'CCT
Cuts at: 313 682 313
Size: 369 3326
StyI C'CwwG_G
Cuts at: 107 137 609 829 1183 107
Size: 30 472 220 354 2619
Fragments arranged by size:
2619 472 354 220 30
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1278 1411 1870 2079 1278
Size: 133 459 209 2894
Fragments arranged by size:
2894 459 209 133
TatI w'GTAC_w
Cuts at: 212 3210 212
Size: 2998 697
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 328 360 3212 3244 328
Size: 32 2852 32 779
Fragments arranged by size:
2852 779 32 32
Tth111I GACn'n_nGTC
Cuts at: 1492 1492
Size: 3695
XbaI T'CTAG_A
Cuts at: 726 726
Size: 3695
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1190 1190
Size: 3695
XmaI C'CCGG_G
Cuts at: 715 715
Size: 3695
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AgeI AlwNI ApaI
ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII
BbeI BciVI BclI BglI BglII BlpI Bme1580I BmgBI
BmrI BplI BpmI BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsiEI BsiHKAI BsmBI Bsp1286I
BspEI BspHI BspMI BsrBI BsrDI BsrFI BssSI BstAPI
BstBI BstYI BtgI BtgZI BtsI DraI DrdI EaeI
EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI
FalI FspI HaeII Hin4I HincII KasI KpnI MluI
MmeI MscI MslI MspA1I NaeI NarI NcoI NgoMIV
NruI NspI PciI PmeI PpiI PspOMI PstI PvuI
PvuII RsrII SacI SalI SapI SbfI ScaI SfcI
SfoI SgfI SmaI SmlI SpeI SphI StuI StyI
TaqII TatI TstI Tth111I XbaI XcmI XmaI
Enzymes that do not cut:
AarI AatII AfeI AflII AhdI AleI AlfI AloI
AscI AvrII BaeI BbsI BbvCI BcgI BfrBI BmtI
Bpu10I BsgI BsiWI BsmI BsrGI BssHII BstEII BstXI
BstZ17I Bsu36I ClaI CspCI DraIII EcoRV FseI FspAI
HindIII HpaI MfeI NdeI NheI NotI NsiI PacI
PasI PflMI PfoI PmlI PpuMI PshAI PsiI PspXI
PsrI SacII SanDI SexAI SfiI SgrAI SnaBI SrfI
SspI SwaI XhoI XmnI ZraI