Restriction map for
pF1KE0214
(Circular) (Six-base) MAPSORT of: pF1KE0214.seq Check: 2085 from: 1 to: 4496
pF1KE0214 4496 bp
With 182 enzymes: SgfI *
April 24, 2009 13:11 ..
AccI GT'mk_AC
Cuts at: 367 1534 367
Size: 1167 3329
Acc65I G'GTAC_C
Cuts at: 1252 1346 1512 1252
Size: 94 166 4236
Fragments arranged by size:
4236 166 94
AclI AA'CG_TT
Cuts at: 4336 4336
Size: 4496
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1466 2321 2753 3500 3768 1466
Size: 855 432 747 268 2194
Fragments arranged by size:
2194 855 747 432 268
AflIII A'CryG_T
Cuts at: 1848 2952 3929 1848
Size: 1104 977 2415
Fragments arranged by size:
2415 1104 977
AgeI A'CCGG_T
Cuts at: 166 2884 166
Size: 2718 1778
AleI CACnn'nnGTG
Cuts at: 1192 1192
Size: 4496
AlwNI CAG_nnn'CTG
Cuts at: 1336 1801 3368 1336
Size: 465 1567 2464
Fragments arranged by size:
2464 1567 465
ApaLI G'TGCA_C
Cuts at: 3266 3266
Size: 4496
ApoI r'AATT_y
Cuts at: 409 1322 1500 3849 4105 409
Size: 913 178 2349 256 800
Fragments arranged by size:
2349 913 800 256 178
AseI AT'TA_AT
Cuts at: 20 349 20
Size: 329 4167
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4496
AvaI C'yCGr_G
Cuts at: 1516 1516
Size: 4496
AvrII C'CTAG_G
Cuts at: 1044 1044
Size: 4496
BamHI G'GATC_C
Cuts at: 1521 1521
Size: 4496
BanI G'GyrC_C
Cuts at: 1173 1252 1346 1512 1926 2174 2209 1173
Size: 79 94 166 414 248 35 3460
Fragments arranged by size:
3460 414 248 166 94 79 35
BanII G_rGCy'C
Cuts at: 1510 2540 1510
Size: 1030 3466
BbeI G_GCGC'C
Cuts at: 1930 2178 1930
Size: 248 4248
BbsI GAAGACnn'nnnn_
Cuts at: 419 419
Size: 4496
BciVI GTATCCnnnnn_n'
Cuts at: 2394 3155 4496 2394
Size: 761 1341 2394
Fragments arranged by size:
2394 1341 761
BclI T'GATC_A
Cuts at: 393 576 2016 393
Size: 183 1440 2873
Fragments arranged by size:
2873 1440 183
BglII A'GATC_T
Cuts at: 2011 4048 2011
Size: 2037 2459
BlpI GC'TnA_GC
Cuts at: 1608 1608
Size: 4496
Bme1580I G_kGCm'C
Cuts at: 2121 2214 3270 2121
Size: 93 1056 3347
Fragments arranged by size:
3347 1056 93
BmrI ACTGGGnnnn_n'
Cuts at: 1708 1789 2124 4268 1708
Size: 81 335 2144 1936
Fragments arranged by size:
2144 1936 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2863 2863
Size: 4496
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1672 2551 3043 3341 1672
Size: 879 492 298 2827
Fragments arranged by size:
2827 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 4200 4200
Size: 4496
BsaAI yAC'GTr
Cuts at: 1849 1849
Size: 4496
BsaBI GATnn'nnATC
Cuts at: 6 2010 4047 6
Size: 2004 2037 455
Fragments arranged by size:
2037 2004 455
BsaHI Gr'CG_yC
Cuts at: 1927 2175 2877 4373 1927
Size: 248 702 1496 2050
Fragments arranged by size:
2050 1496 702 248
BsaWI w'CCGG_w
Cuts at: 166 1685 2206 2884 3158 3305 3716 166
Size: 1519 521 678 274 147 411 946
Fragments arranged by size:
1519 946 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 755 785 755
Size: 30 4466
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1307 1307
Size: 4496
BseYI C'CCAG_C
Cuts at: 1921 2679 3256 3752 1921
Size: 758 577 496 2665
Fragments arranged by size:
2665 758 577 496
BsiEI CG_ry'CG
Cuts at: 104 2038 2084 3292 104
Size: 1934 46 1208 1308
Fragments arranged by size:
1934 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 441 1510 2288 2478 3270 441
Size: 1069 778 190 792 1667
Fragments arranged by size:
1667 1069 792 778 190
BsmI GAATG_Cn'
Cuts at: 286 864 286
Size: 578 3918
BsmBI CGTCTCn'nnnn_
Cuts at: 867 867
Size: 4496
Bsp1286I G_dGCh'C
Cuts at: 441 1510 2121 2214 2288 2478 2540 3270 441
Size: 1069 611 93 74 190 62 730 1667
Fragments arranged by size:
1667 1069 730 611 190 93 74 62
BspEI T'CCGG_A
Cuts at: 3716 3716
Size: 4496
BspHI T'CATG_A
Cuts at: 3672 3672
Size: 4496
BspMI ACCTGCnnnn'nnnn_
Cuts at: 138 1546 1760 2062 2443 138
Size: 1408 214 302 381 2191
Fragments arranged by size:
2191 1408 381 302 214
BsrBI CCG'CTC
Cuts at: 50 2788 2842 4326 4491 50
Size: 2738 54 1484 165 55
Fragments arranged by size:
2738 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 935 2408 935
Size: 1473 3023
BsrFI r'CCGG_y
Cuts at: 166 2494 2675 2884 166
Size: 2328 181 209 1778
Fragments arranged by size:
2328 1778 209 181
BssSI C'ACGA_G
Cuts at: 2767 3125 2767
Size: 358 4138
BstAPI GCAn_nnn'nTGC
Cuts at: 1873 1873
Size: 4496
BstBI TT'CG_AA
Cuts at: 609 2857 609
Size: 2248 2248
BstXI CCAn_nnnn'nTGG
Cuts at: 980 980
Size: 4496
BstYI r'GATC_y
Cuts at: 1 765 1521 1988 2011 2346 2592 3604 3690
Size: 764 756 467 23 335 246 1012 86
Cuts at: 3690 3702 4048 1
Size: 12 346 449
Fragments arranged by size:
1012 764 756 467 449 346 335 246
86 23 12
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 2446 2627 2790 2446
Size: 181 163 4152
Fragments arranged by size:
4152 181 163
BtsI GCAGTG_nn'
Cuts at: 1298 1739 1298
Size: 441 4055
DraI TTT'AAA
Cuts at: 1495 1495
Size: 4496
DrdI GACnn_nn'nnGTC
Cuts at: 2202 3060 2202
Size: 858 3638
EaeI y'GGCC_r
Cuts at: 2081 2255 2646 2673 4018 2081
Size: 174 391 27 1345 2559
Fragments arranged by size:
2559 1345 391 174 27
EagI C'GGCC_G
Cuts at: 2081 2081
Size: 4496
EarI CTCTTCn'nnn_
Cuts at: 2519 2729 2519
Size: 210 4286
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2704 3014 3160 4306 2704
Size: 310 146 1146 2894
Fragments arranged by size:
2894 1146 310 146
EcoICRI GAG'CTC
Cuts at: 1508 1508
Size: 4496
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1466 2321 2753 3500 3768 1466
Size: 855 432 747 268 2194
Fragments arranged by size:
2194 855 747 432 268
EcoO109I rG'GnC_Cy
Cuts at: 1635 1635
Size: 4496
EcoRI G'AATT_C
Cuts at: 1500 1500
Size: 4496
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2515 2515
Size: 4496
FspI TGC'GCA
Cuts at: 2277 2277
Size: 4496
HaeII r_GCGC'y
Cuts at: 1930 2178 3200 4179 1930
Size: 248 1022 979 2247
Fragments arranged by size:
2247 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 769 801 869 901 2338 2368 2370 2400 769
Size: 32 68 32 1437 30 2 30 2865
Fragments arranged by size:
2865 1437 68 32 32 30 30 2
HincII GTy'rAC
Cuts at: 1535 1535
Size: 4496
KasI G'GCGC_C
Cuts at: 1926 2174 1926
Size: 248 4248
KpnI G_GTAC'C
Cuts at: 1256 1350 1516 1256
Size: 94 166 4236
Fragments arranged by size:
4236 166 94
MluI A'CGCG_T
Cuts at: 3929 3929
Size: 4496
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 3167 3351 3167
Size: 184 4312
MscI TGG'CCA
Cuts at: 2257 2257
Size: 4496
MslI CAynn'nnrTG
Cuts at: 1192 2485 2612 1192
Size: 1293 127 3076
Fragments arranged by size:
3076 1293 127
MspA1I CmG'CkG
Cuts at: 1427 1607 1921 2281 3294 3539 1427
Size: 180 314 360 1013 245 2384
Fragments arranged by size:
2384 1013 360 314 245 180
NaeI GCC'GGC
Cuts at: 2677 2677
Size: 4496
NarI GG'CG_CC
Cuts at: 1927 2175 1927
Size: 248 4248
NdeI CA'TA_TG
Cuts at: 1085 1085
Size: 4496
NgoMIV G'CCGG_C
Cuts at: 2675 2675
Size: 4496
NspI r_CATG'y
Cuts at: 849 1549 2049 2956 849
Size: 700 500 907 2389
Fragments arranged by size:
2389 907 700 500
PciI A'CATG_T
Cuts at: 2952 2952
Size: 4496
PflMI CCAn_nnn'nTGG
Cuts at: 455 548 455
Size: 93 4403
PfoI T'CCnGG_A
Cuts at: 290 290
Size: 4496
PmeI GTTT'AAAC
Cuts at: 1495 1495
Size: 4496
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3635 3667 3635
Size: 32 4464
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 1439 1471 1439
Size: 32 4464
PstI C_TGCA'G
Cuts at: 332 1305 1543 2228 332
Size: 973 238 685 2600
Fragments arranged by size:
2600 973 685 238
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4496
PvuII CAG'CTG
Cuts at: 1427 1921 2281 1427
Size: 494 360 3642
Fragments arranged by size:
3642 494 360
RsrII CG'GwC_CG
Cuts at: 2691 2691
Size: 4496
SacI G_AGCT'C
Cuts at: 1510 1510
Size: 4496
SalI G'TCGA_C
Cuts at: 1533 1533
Size: 4496
SapI GCTCTTCn'nnn_
Cuts at: 2519 2729 2519
Size: 210 4286
SbfI CC_TGCA'GG
Cuts at: 1543 1543
Size: 4496
ScaI AGT'ACT
Cuts at: 4013 4013
Size: 4496
SfcI C'TryA_G
Cuts at: 33 328 1301 1539 2224 3217 3408 33
Size: 295 973 238 685 993 191 1121
Fragments arranged by size:
1121 993 973 685 295 238 191
SfoI GGC'GCC
Cuts at: 1928 2176 1928
Size: 248 4248
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4496
SmaI CCC'GGG
Cuts at: 1518 1518
Size: 4496
SmlI C'TyrA_G
Cuts at: 1651 2566 3058 3320 1651
Size: 915 492 262 2827
Fragments arranged by size:
2827 915 492 262
SpeI A'CTAG_T
Cuts at: 67 1036 67
Size: 969 3527
SphI G_CATG'C
Cuts at: 1549 2049 1549
Size: 500 3996
SspI AAT'ATT
Cuts at: 246 246
Size: 4496
StyI C'CwwG_G
Cuts at: 1044 1630 1984 1044
Size: 586 354 3556
Fragments arranged by size:
3556 586 354
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 2079 2212 2671 2880 2079
Size: 133 459 209 3695
Fragments arranged by size:
3695 459 209 133
TatI w'GTAC_w
Cuts at: 1144 4011 1144
Size: 2867 1629
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 755 787 4013 4045 755
Size: 32 3226 32 1206
Fragments arranged by size:
3226 1206 32 32
Tth111I GACn'n_nGTC
Cuts at: 2293 2293
Size: 4496
XbaI T'CTAG_A
Cuts at: 1527 1527
Size: 4496
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1991 1991
Size: 4496
XmaI C'CCGG_G
Cuts at: 1516 1516
Size: 4496
XmnI GAAnn'nnTTC
Cuts at: 1079 1079
Size: 4496
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AgeI AleI AlwNI
ApaLI ApoI AseI AsiSI AvaI AvrII BamHI BanI
BanII BbeI BbsI BciVI BclI BglII BlpI Bme1580I
BmrI BplI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BsaXI BseRI BseYI BsiEI BsiHKAI BsmI BsmBI Bsp1286I
BspEI BspHI BspMI BsrBI BsrDI BsrFI BssSI BstAPI
BstBI BstXI BstYI BtgZI BtsI DraI DrdI EaeI
EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI FalI
FspI HaeII Hin4I HincII KasI KpnI MluI MmeI
MscI MslI MspA1I NaeI NarI NdeI NgoMIV NspI
PciI PflMI PfoI PmeI PpiI PsrI PstI PvuI
PvuII RsrII SacI SalI SapI SbfI ScaI SfcI
SfoI SgfI SmaI SmlI SpeI SphI SspI StyI
TaqII TatI TstI Tth111I XbaI XcmI XmaI XmnI
Enzymes that do not cut:
AarI AatII AfeI AflII AhdI AlfI AloI ApaI
AscI BaeI BbvCI BcgI BfrBI BglI BmgBI BmtI
BpmI Bpu10I BsgI BsiWI BsrGI BssHII BstEII BstZ17I
Bsu36I BtgI ClaI CspCI DraIII EcoNI EcoRV FseI
FspAI HindIII HpaI MfeI NcoI NheI NotI NruI
NsiI PacI PasI PmlI PpuMI PshAI PsiI PspOMI
PspXI SacII SanDI SexAI SfiI SgrAI SnaBI SrfI
StuI SwaI XhoI ZraI