Restriction map for
pF1KE0208
(Circular) (Six-base) MAPSORT of: pF1KE0208.seq Check: 8975 from: 1 to: 4226
pF1KE0208 4226 bp
With 182 enzymes: SgfI *
April 24, 2009 13:11 ..
AccI GT'mk_AC
Cuts at: 1019 1264 1019
Size: 245 3981
Acc65I G'GTAC_C
Cuts at: 1242 1242
Size: 4226
AclI AA'CG_TT
Cuts at: 4066 4066
Size: 4226
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 2051 2483 3230 3498 2051
Size: 432 747 268 2779
Fragments arranged by size:
2779 747 432 268
AflIII A'CryG_T
Cuts at: 341 1578 2682 3659 341
Size: 1237 1104 977 908
Fragments arranged by size:
1237 1104 977 908
AgeI A'CCGG_T
Cuts at: 2614 2614
Size: 4226
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 586 586
Size: 4226
AlwNI CAG_nnn'CTG
Cuts at: 324 549 1531 3098 324
Size: 225 982 1567 1452
Fragments arranged by size:
1567 1452 982 225
ApaI G_GGCC'C
Cuts at: 425 425
Size: 4226
ApaLI G'TGCA_C
Cuts at: 2996 2996
Size: 4226
ApoI r'AATT_y
Cuts at: 633 1048 1230 3579 3835 633
Size: 415 182 2349 256 1024
Fragments arranged by size:
2349 1024 415 256 182
AseI AT'TA_AT
Cuts at: 20 20
Size: 4226
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4226
AvaI C'yCGr_G
Cuts at: 355 791 1246 355
Size: 436 455 3335
Fragments arranged by size:
3335 455 436
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 963 996 963
Size: 33 4193
BamHI G'GATC_C
Cuts at: 1251 1251
Size: 4226
BanI G'GyrC_C
Cuts at: 510 1242 1656 1904 1939 510
Size: 732 414 248 35 2797
Fragments arranged by size:
2797 732 414 248 35
BanII G_rGCy'C
Cuts at: 425 1240 2270 425
Size: 815 1030 2381
Fragments arranged by size:
2381 1030 815
BbeI G_GCGC'C
Cuts at: 1660 1908 1660
Size: 248 3978
BbsI GAAGACnn'nnnn_
Cuts at: 754 754
Size: 4226
BbvCI CC'TCA_GC
Cuts at: 169 487 169
Size: 318 3908
BciVI GTATCCnnnnn_n'
Cuts at: 2124 2885 4226 2124
Size: 761 1341 2124
Fragments arranged by size:
2124 1341 761
BclI T'GATC_A
Cuts at: 1746 1746
Size: 4226
BglI GCCn_nnn'nGGC
Cuts at: 145 560 145
Size: 415 3811
BglII A'GATC_T
Cuts at: 1741 3778 1741
Size: 2037 2189
BlpI GC'TnA_GC
Cuts at: 1338 1338
Size: 4226
Bme1580I G_kGCm'C
Cuts at: 425 1851 1944 3000 425
Size: 1426 93 1056 1651
Fragments arranged by size:
1651 1426 1056 93
BmgBI CAC'GTC
Cuts at: 330 330
Size: 4226
BmrI ACTGGGnnnn_n'
Cuts at: 419 444 1438 1519 1854 3998 419
Size: 25 994 81 335 2144 647
Fragments arranged by size:
2144 994 647 335 81 25
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 817 2593 817
Size: 1776 2450
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 211 223 301 457 211
Size: 12 78 156 3980
Fragments arranged by size:
3980 156 78 12
Bpu10I CC'TnA_GC
Cuts at: 169 487 550 169
Size: 318 63 3845
Fragments arranged by size:
3845 318 63
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1402 2281 2773 3071 1402
Size: 879 492 298 2557
Fragments arranged by size:
2557 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 3930 3930
Size: 4226
BsaAI yAC'GTr
Cuts at: 1579 1579
Size: 4226
BsaBI GATnn'nnATC
Cuts at: 6 1740 3777 6
Size: 1734 2037 455
Fragments arranged by size:
2037 1734 455
BsaHI Gr'CG_yC
Cuts at: 1657 1905 2607 4103 1657
Size: 248 702 1496 1780
Fragments arranged by size:
1780 1496 702 248
BsaWI w'CCGG_w
Cuts at: 1415 1936 2614 2888 3035 3446 1415
Size: 521 678 274 147 411 2195
Fragments arranged by size:
2195 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 113 143 113
Size: 30 4196
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 127 232 713 1028 127
Size: 105 481 315 3325
Fragments arranged by size:
3325 481 315 105
BseYI C'CCAG_C
Cuts at: 294 1651 2409 2986 3482 294
Size: 1357 758 577 496 1038
Fragments arranged by size:
1357 1038 758 577 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 890 1175 890
Size: 285 3941
BsiEI CG_ry'CG
Cuts at: 104 140 1768 1814 3022 104
Size: 36 1628 46 1208 1308
Fragments arranged by size:
1628 1308 1208 46 36
BsiHKAI G_wGCw'C
Cuts at: 482 1240 2018 2208 3000 482
Size: 758 778 190 792 1708
Fragments arranged by size:
1708 792 778 758 190
BsmI GAATG_Cn'
Cuts at: 1205 1205
Size: 4226
BsmBI CGTCTCn'nnnn_
Cuts at: 178 178
Size: 4226
Bsp1286I G_dGCh'C
Cuts at: 425 482 1240 1851 1944 2018 2208 2270 3000
Size: 57 758 611 93 74 190 62 730
Cuts at: 3000 425
Size: 1651
Fragments arranged by size:
1651 758 730 611 190 93 74 62
57
BspEI T'CCGG_A
Cuts at: 3446 3446
Size: 4226
BspHI T'CATG_A
Cuts at: 3402 3402
Size: 4226
BspMI ACCTGCnnnn'nnnn_
Cuts at: 1276 1490 1792 2173 1276
Size: 214 302 381 3329
Fragments arranged by size:
3329 381 302 214
BsrBI CCG'CTC
Cuts at: 50 222 335 459 2518 2572 4056 4221 50
Size: 172 113 124 2059 54 1484 165 55
Fragments arranged by size:
2059 1484 172 165 124 113 55 54
BsrDI GCAATG_nn'
Cuts at: 2138 2138
Size: 4226
BsrFI r'CCGG_y
Cuts at: 410 2224 2405 2614 410
Size: 1814 181 209 2022
Fragments arranged by size:
2022 1814 209 181
BssHII G'CGCG_C
Cuts at: 534 536 534
Size: 2 4224
BssSI C'ACGA_G
Cuts at: 2497 2855 2497
Size: 358 3868
BstAPI GCAn_nnn'nTGC
Cuts at: 1603 1603
Size: 4226
BstBI TT'CG_AA
Cuts at: 924 2587 924
Size: 1663 2563
BstXI CCAn_nnnn'nTGG
Cuts at: 444 444
Size: 4226
BstYI r'GATC_y
Cuts at: 1 797 1251 1718 1741 2076 2322 3334 3420
Size: 796 454 467 23 335 246 1012 86
Cuts at: 3420 3432 3778 1
Size: 12 346 449
Fragments arranged by size:
1012 796 467 454 449 346 335 246
86 23 12
BtgI C'CryG_G
Cuts at: 107 286 107
Size: 179 4047
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 2176 2357 2520 2176
Size: 181 163 3882
Fragments arranged by size:
3882 181 163
BtsI GCAGTG_nn'
Cuts at: 1469 1469
Size: 4226
DraI TTT'AAA
Cuts at: 1225 1225
Size: 4226
DrdI GACnn_nn'nnGTC
Cuts at: 192 1033 1932 2790 192
Size: 841 899 858 1628
Fragments arranged by size:
1628 899 858 841
EaeI y'GGCC_r
Cuts at: 137 398 1811 1985 2376 2403 3748 137
Size: 261 1413 174 391 27 1345 615
Fragments arranged by size:
1413 1345 615 391 261 174 27
EagI C'GGCC_G
Cuts at: 137 1811 137
Size: 1674 2552
EarI CTCTTCn'nnn_
Cuts at: 815 2249 2459 815
Size: 1434 210 2582
Fragments arranged by size:
2582 1434 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2434 2744 2890 4036 2434
Size: 310 146 1146 2624
Fragments arranged by size:
2624 1146 310 146
EcoICRI GAG'CTC
Cuts at: 1238 1238
Size: 4226
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 211 223 301 457 2051 2483 3230 3498 211
Size: 12 78 156 1594 432 747 268 939
Fragments arranged by size:
1594 939 747 432 268 156 78 12
EcoO109I rG'GnC_Cy
Cuts at: 441 1365 441
Size: 924 3302
EcoRI G'AATT_C
Cuts at: 1230 1230
Size: 4226
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 1231 2245 1231
Size: 1014 3212
FspI TGC'GCA
Cuts at: 388 2007 388
Size: 1619 2607
HaeII r_GCGC'y
Cuts at: 244 295 1660 1908 2930 3909 244
Size: 51 1365 248 1022 979 561
Fragments arranged by size:
1365 1022 979 561 248 51
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 2068 2098 2100 2130 2068
Size: 30 2 30 4164
Fragments arranged by size:
4164 30 30 2
HincII GTy'rAC
Cuts at: 347 1265 347
Size: 918 3308
KasI G'GCGC_C
Cuts at: 1656 1904 1656
Size: 248 3978
KpnI G_GTAC'C
Cuts at: 1246 1246
Size: 4226
MfeI C'AATT_G
Cuts at: 990 990
Size: 4226
MluI A'CGCG_T
Cuts at: 341 3659 341
Size: 3318 908
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 910 2897 3081 910
Size: 1987 184 2055
Fragments arranged by size:
2055 1987 184
MscI TGG'CCA
Cuts at: 400 1987 400
Size: 1587 2639
MslI CAynn'nnrTG
Cuts at: 1107 2215 2342 1107
Size: 1108 127 2991
Fragments arranged by size:
2991 1108 127
MspA1I CmG'CkG
Cuts at: 127 302 321 491 1337 1651 2011 3024 3269
Size: 175 19 170 846 314 360 1013 245
Cuts at: 3269 127
Size: 1084
Fragments arranged by size:
1084 1013 846 360 314 245 175 170
19
NaeI GCC'GGC
Cuts at: 2407 2407
Size: 4226
NarI GG'CG_CC
Cuts at: 1657 1905 1657
Size: 248 3978
NcoI C'CATG_G
Cuts at: 107 107
Size: 4226
NgoMIV G'CCGG_C
Cuts at: 2405 2405
Size: 4226
NspI r_CATG'y
Cuts at: 1279 1779 2686 1279
Size: 500 907 2819
Fragments arranged by size:
2819 907 500
PciI A'CATG_T
Cuts at: 2682 2682
Size: 4226
PflMI CCAn_nnn'nTGG
Cuts at: 324 1009 324
Size: 685 3541
PfoI T'CCnGG_A
Cuts at: 503 893 954 503
Size: 390 61 3775
Fragments arranged by size:
3775 390 61
PmeI GTTT'AAAC
Cuts at: 1225 1225
Size: 4226
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3365 3397 3365
Size: 32 4194
PspOMI G'GGCC_C
Cuts at: 421 421
Size: 4226
PstI C_TGCA'G
Cuts at: 545 575 1273 1958 545
Size: 30 698 685 2813
Fragments arranged by size:
2813 698 685 30
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4226
PvuII CAG'CTG
Cuts at: 321 1651 2011 321
Size: 1330 360 2536
Fragments arranged by size:
2536 1330 360
RsrII CG'GwC_CG
Cuts at: 2421 2421
Size: 4226
SacI G_AGCT'C
Cuts at: 1240 1240
Size: 4226
SalI G'TCGA_C
Cuts at: 1263 1263
Size: 4226
SapI GCTCTTCn'nnn_
Cuts at: 2249 2459 2249
Size: 210 4016
SbfI CC_TGCA'GG
Cuts at: 1273 1273
Size: 4226
ScaI AGT'ACT
Cuts at: 3743 3743
Size: 4226
SfcI C'TryA_G
Cuts at: 33 541 571 1269 1954 2947 3138 33
Size: 508 30 698 685 993 191 1121
Fragments arranged by size:
1121 993 698 685 508 191 30
SfiI GGCCn_nnn'nGGCC
Cuts at: 145 145
Size: 4226
SfoI GGC'GCC
Cuts at: 1658 1906 1658
Size: 248 3978
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4226
SmaI CCC'GGG
Cuts at: 1248 1248
Size: 4226
SmlI C'TyrA_G
Cuts at: 791 1381 2296 2788 3050 791
Size: 590 915 492 262 1967
Fragments arranged by size:
1967 915 590 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4226
SphI G_CATG'C
Cuts at: 1279 1779 1279
Size: 500 3726
SspI AAT'ATT
Cuts at: 930 930
Size: 4226
StyI C'CwwG_G
Cuts at: 107 437 1360 1714 107
Size: 330 923 354 2619
Fragments arranged by size:
2619 923 354 330
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1809 1942 2401 2610 1809
Size: 133 459 209 3425
Fragments arranged by size:
3425 459 209 133
TatI w'GTAC_w
Cuts at: 3741 3741
Size: 4226
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 113 145 3743 3775 113
Size: 32 3598 32 564
Fragments arranged by size:
3598 564 32 32
Tth111I GACn'n_nGTC
Cuts at: 2023 2023
Size: 4226
XbaI T'CTAG_A
Cuts at: 1257 1257
Size: 4226
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 186 1721 186
Size: 1535 2691
XhoI C'TCGA_G
Cuts at: 791 791
Size: 4226
XmaI C'CCGG_G
Cuts at: 1246 1246
Size: 4226
XmnI GAAnn'nnTTC
Cuts at: 930 930
Size: 4226
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AgeI AlfI AlwNI
ApaI ApaLI ApoI AseI AsiSI AvaI BaeI BamHI
BanI BanII BbeI BbsI BbvCI BciVI BclI BglI
BglII BlpI Bme1580I BmgBI BmrI BplI BpmI Bpu10I
BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI
BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI
BspHI BspMI BsrBI BsrDI BsrFI BssHII BssSI BstAPI
BstBI BstXI BstYI BtgI BtgZI BtsI DraI DrdI
EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI
FalI FspI HaeII Hin4I HincII KasI KpnI MfeI
MluI MmeI MscI MslI MspA1I NaeI NarI NcoI
NgoMIV NspI PciI PflMI PfoI PmeI PpiI PspOMI
PstI PvuI PvuII RsrII SacI SalI SapI SbfI
ScaI SfcI SfiI SfoI SgfI SmaI SmlI SpeI
SphI SspI StyI TaqII TatI TstI Tth111I XbaI
XcmI XhoI XmaI XmnI
Enzymes that do not cut:
AarI AatII AfeI AflII AhdI AleI AloI AscI
AvrII BcgI BfrBI BmtI BsiWI BsrGI BstEII BstZ17I
Bsu36I ClaI CspCI DraIII EcoNI EcoRV FseI FspAI
HindIII HpaI NdeI NheI NotI NruI NsiI PacI
PasI PmlI PpuMI PshAI PsiI PspXI PsrI SacII
SanDI SexAI SgrAI SnaBI SrfI StuI SwaI ZraI