Restriction map for
pF1KE0193
(Circular) (Six-base) MAPSORT of: pF1KE0193.seq Check: 6146 from: 1 to: 4190
pF1KE0193 4190 bp
With 182 enzymes: SgfI *
April 24, 2009 13:11 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 432 432
Size: 4190
AccI GT'mk_AC
Cuts at: 581 1228 581
Size: 647 3543
Acc65I G'GTAC_C
Cuts at: 1206 1206
Size: 4190
AclI AA'CG_TT
Cuts at: 247 4030 247
Size: 3783 407
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 2015 2447 3194 3462 2015
Size: 432 747 268 2743
Fragments arranged by size:
2743 747 432 268
AfeI AGC'GCT
Cuts at: 1177 1177
Size: 4190
AflIII A'CryG_T
Cuts at: 1542 2646 3623 1542
Size: 1104 977 2109
Fragments arranged by size:
2109 1104 977
AgeI A'CCGG_T
Cuts at: 2578 2578
Size: 4190
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 874 874
Size: 4190
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 670 702 670
Size: 32 4158
AlwNI CAG_nnn'CTG
Cuts at: 535 1495 3062 535
Size: 960 1567 1663
Fragments arranged by size:
1663 1567 960
ApaLI G'TGCA_C
Cuts at: 445 2960 445
Size: 2515 1675
ApoI r'AATT_y
Cuts at: 556 1194 3543 3799 556
Size: 638 2349 256 947
Fragments arranged by size:
2349 947 638 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 4190
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4190
AvaI C'yCGr_G
Cuts at: 167 1210 167
Size: 1043 3147
BamHI G'GATC_C
Cuts at: 1215 1215
Size: 4190
BanI G'GyrC_C
Cuts at: 394 411 895 1206 1620 1868 1903 394
Size: 17 484 311 414 248 35 2681
Fragments arranged by size:
2681 484 414 311 248 35 17
BanII G_rGCy'C
Cuts at: 1204 2234 1204
Size: 1030 3160
BbeI G_GCGC'C
Cuts at: 1624 1872 1624
Size: 248 3942
BbvCI CC'TCA_GC
Cuts at: 691 691
Size: 4190
BciVI GTATCCnnnnn_n'
Cuts at: 2088 2849 4190 2088
Size: 761 1341 2088
Fragments arranged by size:
2088 1341 761
BclI T'GATC_A
Cuts at: 699 1710 699
Size: 1011 3179
BglI GCCn_nnn'nGGC
Cuts at: 112 112
Size: 4190
BglII A'GATC_T
Cuts at: 1705 3742 1705
Size: 2037 2153
BlpI GC'TnA_GC
Cuts at: 1302 1302
Size: 4190
Bme1580I G_kGCm'C
Cuts at: 304 449 863 1815 1908 2964 304
Size: 145 414 952 93 1056 1530
Fragments arranged by size:
1530 1056 952 414 145 93
BmrI ACTGGGnnnn_n'
Cuts at: 348 441 920 1402 1483 1818 3962 348
Size: 93 479 482 81 335 2144 576
Fragments arranged by size:
2144 576 482 479 335 93 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2557 2557
Size: 4190
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 620 620
Size: 4190
Bpu10I CC'TnA_GC
Cuts at: 691 691
Size: 4190
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1366 2245 2737 3035 1366
Size: 879 492 298 2521
Fragments arranged by size:
2521 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 3894 3894
Size: 4190
BsaAI yAC'GTr
Cuts at: 1543 1543
Size: 4190
BsaBI GATnn'nnATC
Cuts at: 6 1704 3741 6
Size: 1698 2037 455
Fragments arranged by size:
2037 1698 455
BsaHI Gr'CG_yC
Cuts at: 1621 1869 2571 4067 1621
Size: 248 702 1496 1744
Fragments arranged by size:
1744 1496 702 248
BsaWI w'CCGG_w
Cuts at: 1379 1900 2578 2852 2999 3410 1379
Size: 521 678 274 147 411 2159
Fragments arranged by size:
2159 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 563 593 670 700 563
Size: 30 77 30 4053
Fragments arranged by size:
4053 77 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 613 891 613
Size: 278 3912
BseYI C'CCAG_C
Cuts at: 567 729 1615 2373 2950 3446 567
Size: 162 886 758 577 496 1311
Fragments arranged by size:
1311 886 758 577 496 162
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 723 723
Size: 4190
BsiEI CG_ry'CG
Cuts at: 104 1732 1778 2986 104
Size: 1628 46 1208 1308
Fragments arranged by size:
1628 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 272 449 1204 1982 2172 2964 272
Size: 177 755 778 190 792 1498
Fragments arranged by size:
1498 792 778 755 190 177
Bsp1286I G_dGCh'C
Cuts at: 272 304 449 863 1204 1815 1908 1982 2172
Size: 32 145 414 341 611 93 74 190
Cuts at: 2172 2234 2964 272
Size: 62 730 1498
Fragments arranged by size:
1498 730 611 414 341 190 145 93
74 62 32
BspEI T'CCGG_A
Cuts at: 3410 3410
Size: 4190
BspHI T'CATG_A
Cuts at: 702 930 3366 702
Size: 228 2436 1526
Fragments arranged by size:
2436 1526 228
BspMI ACCTGCnnnn'nnnn_
Cuts at: 432 1240 1454 1756 2137 432
Size: 808 214 302 381 2485
Fragments arranged by size:
2485 808 381 302 214
BsrBI CCG'CTC
Cuts at: 50 708 2482 2536 4020 4185 50
Size: 658 1774 54 1484 165 55
Fragments arranged by size:
1774 1484 658 165 55 54
BsrDI GCAATG_nn'
Cuts at: 342 2102 342
Size: 1760 2430
BsrFI r'CCGG_y
Cuts at: 2188 2369 2578 2188
Size: 181 209 3800
Fragments arranged by size:
3800 209 181
BssSI C'ACGA_G
Cuts at: 2461 2819 2461
Size: 358 3832
BstAPI GCAn_nnn'nTGC
Cuts at: 1567 1567
Size: 4190
BstBI TT'CG_AA
Cuts at: 2551 2551
Size: 4190
BstYI r'GATC_y
Cuts at: 1 915 1215 1682 1705 2040 2286 3298 3384
Size: 914 300 467 23 335 246 1012 86
Cuts at: 3384 3396 3742 1
Size: 12 346 449
Fragments arranged by size:
1012 914 467 449 346 335 300 246
86 23 12
BtgI C'CryG_G
Cuts at: 107 341 459 671 879 107
Size: 234 118 212 208 3418
Fragments arranged by size:
3418 234 212 208 118
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 168 2140 2321 2484 168
Size: 1972 181 163 1874
Fragments arranged by size:
1972 1874 181 163
BtsI GCAGTG_nn'
Cuts at: 151 1054 1433 151
Size: 903 379 2908
Fragments arranged by size:
2908 903 379
DraI TTT'AAA
Cuts at: 1189 1189
Size: 4190
DrdI GACnn_nn'nnGTC
Cuts at: 1896 2754 1896
Size: 858 3332
EaeI y'GGCC_r
Cuts at: 1775 1949 2340 2367 3712 1775
Size: 174 391 27 1345 2253
Fragments arranged by size:
2253 1345 391 174 27
EagI C'GGCC_G
Cuts at: 1775 1775
Size: 4190
EarI CTCTTCn'nnn_
Cuts at: 616 977 1087 2213 2423 616
Size: 361 110 1126 210 2383
Fragments arranged by size:
2383 1126 361 210 110
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 352 859 2398 2708 2854 4000 352
Size: 507 1539 310 146 1146 542
Fragments arranged by size:
1539 1146 542 507 310 146
EcoICRI GAG'CTC
Cuts at: 1202 1202
Size: 4190
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 620 2015 2447 3194 3462 620
Size: 1395 432 747 268 1348
Fragments arranged by size:
1395 1348 747 432 268
EcoO109I rG'GnC_Cy
Cuts at: 124 532 1329 124
Size: 408 797 2985
Fragments arranged by size:
2985 797 408
EcoRI G'AATT_C
Cuts at: 1194 1194
Size: 4190
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2209 2209
Size: 4190
FspI TGC'GCA
Cuts at: 1971 1971
Size: 4190
HaeII r_GCGC'y
Cuts at: 1179 1624 1872 2894 3873 1179
Size: 445 248 1022 979 1496
Fragments arranged by size:
1496 1022 979 445 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1035 1067 2032 2062 2064 2094 1035
Size: 32 965 30 2 30 3131
Fragments arranged by size:
3131 965 32 30 30 2
HincII GTy'rAC
Cuts at: 1229 1229
Size: 4190
KasI G'GCGC_C
Cuts at: 1620 1868 1620
Size: 248 3942
KpnI G_GTAC'C
Cuts at: 1210 1210
Size: 4190
MluI A'CGCG_T
Cuts at: 3623 3623
Size: 4190
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 2861 3045 2861
Size: 184 4006
MscI TGG'CCA
Cuts at: 1951 1951
Size: 4190
MslI CAynn'nnrTG
Cuts at: 346 488 856 1001 2179 2306 346
Size: 142 368 145 1178 127 2230
Fragments arranged by size:
2230 1178 368 145 142 127
MspA1I CmG'CkG
Cuts at: 648 881 1301 1615 1975 2988 3233 648
Size: 233 420 314 360 1013 245 1605
Fragments arranged by size:
1605 1013 420 360 314 245 233
NaeI GCC'GGC
Cuts at: 2371 2371
Size: 4190
NarI GG'CG_CC
Cuts at: 1621 1869 1621
Size: 248 3942
NcoI C'CATG_G
Cuts at: 107 341 459 671 107
Size: 234 118 212 3626
Fragments arranged by size:
3626 234 212 118
NgoMIV G'CCGG_C
Cuts at: 2369 2369
Size: 4190
NspI r_CATG'y
Cuts at: 1243 1743 2650 1243
Size: 500 907 2783
Fragments arranged by size:
2783 907 500
PciI A'CATG_T
Cuts at: 2646 2646
Size: 4190
PflMI CCAn_nnn'nTGG
Cuts at: 297 297
Size: 4190
PfoI T'CCnGG_A
Cuts at: 178 636 178
Size: 458 3732
PmeI GTTT'AAAC
Cuts at: 1189 1189
Size: 4190
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 670 702 3329 3361 670
Size: 32 2627 32 1499
Fragments arranged by size:
2627 1499 32 32
PpuMI rG'GwC_Cy
Cuts at: 124 124
Size: 4190
PstI C_TGCA'G
Cuts at: 1237 1922 1237
Size: 685 3505
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4190
PvuII CAG'CTG
Cuts at: 1615 1975 1615
Size: 360 3830
RsrII CG'GwC_CG
Cuts at: 2385 2385
Size: 4190
SacI G_AGCT'C
Cuts at: 1204 1204
Size: 4190
SacII CC_GC'GG
Cuts at: 882 882
Size: 4190
SalI G'TCGA_C
Cuts at: 1227 1227
Size: 4190
SanDI GG'GwC_CC
Cuts at: 124 124
Size: 4190
SapI GCTCTTCn'nnn_
Cuts at: 2213 2423 2213
Size: 210 3980
SbfI CC_TGCA'GG
Cuts at: 1237 1237
Size: 4190
ScaI AGT'ACT
Cuts at: 256 3707 256
Size: 3451 739
SfcI C'TryA_G
Cuts at: 33 722 1233 1918 2911 3102 33
Size: 689 511 685 993 191 1121
Fragments arranged by size:
1121 993 689 685 511 191
SfoI GGC'GCC
Cuts at: 1622 1870 1622
Size: 248 3942
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4190
SmaI CCC'GGG
Cuts at: 1212 1212
Size: 4190
SmlI C'TyrA_G
Cuts at: 1345 2260 2752 3014 1345
Size: 915 492 262 2521
Fragments arranged by size:
2521 915 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4190
SphI G_CATG'C
Cuts at: 1243 1743 1243
Size: 500 3690
StyI C'CwwG_G
Cuts at: 107 129 341 459 671 1324 1678 107
Size: 22 212 118 212 653 354 2619
Fragments arranged by size:
2619 653 354 212 212 118 22
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1773 1906 2365 2574 1773
Size: 133 459 209 3389
Fragments arranged by size:
3389 459 209 133
TatI w'GTAC_w
Cuts at: 254 3705 254
Size: 3451 739
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3707 3739 3707
Size: 32 4158
Tth111I GACn'n_nGTC
Cuts at: 1987 1987
Size: 4190
XbaI T'CTAG_A
Cuts at: 1221 1221
Size: 4190
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1685 1685
Size: 4190
XmaI C'CCGG_G
Cuts at: 1210 1210
Size: 4190
Enzymes that do cut:
AarI AccI Acc65I AclI AcuI AfeI AflIII AgeI
AlfI AloI AlwNI ApaLI ApoI AseI AsiSI AvaI
BamHI BanI BanII BbeI BbvCI BciVI BclI BglI
BglII BlpI Bme1580I BmrI BplI BpmI Bpu10I BpuEI
BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI
BsgI BsiEI BsiHKAI Bsp1286I BspEI BspHI BspMI BsrBI
BsrDI BsrFI BssSI BstAPI BstBI BstYI BtgI BtgZI
BtsI DraI DrdI EaeI EagI EarI EciI EcoICRI
Eco57MI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII
KasI KpnI MluI MmeI MscI MslI MspA1I NaeI
NarI NcoI NgoMIV NspI PciI PflMI PfoI PmeI
PpiI PpuMI PstI PvuI PvuII RsrII SacI SacII
SalI SanDI SapI SbfI ScaI SfcI SfoI SgfI
SmaI SmlI SpeI SphI StyI TaqII TatI TstI
Tth111I XbaI XcmI XmaI
Enzymes that do not cut:
AatII AflII AhdI AleI ApaI AscI AvrII BaeI
BbsI BcgI BfrBI BmgBI BmtI BsiWI BsmI BsmBI
BsrGI BssHII BstEII BstXI BstZ17I Bsu36I ClaI CspCI
DraIII EcoNI EcoRV FseI FspAI HindIII HpaI MfeI
NdeI NheI NotI NruI NsiI PacI PasI PmlI
PshAI PsiI PspOMI PspXI PsrI SexAI SfiI SgrAI
SnaBI SrfI SspI StuI SwaI XhoI XmnI ZraI