Restriction map for
pF1KE0185
(Circular) (Six-base) MAPSORT of: pF1KE0185.seq Check: 697 from: 1 to: 3881
pF1KE0185 3881 bp
With 182 enzymes: SgfI *
April 24, 2009 13:11 ..
AccI GT'mk_AC
Cuts at: 919 919
Size: 3881
Acc65I G'GTAC_C
Cuts at: 897 897
Size: 3881
AclI AA'CG_TT
Cuts at: 3721 3721
Size: 3881
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1706 2138 2885 3153 1706
Size: 432 747 268 2434
Fragments arranged by size:
2434 747 432 268
AflIII A'CryG_T
Cuts at: 613 615 1233 2337 3314 613
Size: 2 618 1104 977 1180
Fragments arranged by size:
1180 1104 977 618 2
AgeI A'CCGG_T
Cuts at: 2269 2269
Size: 3881
AleI CACnn'nnGTG
Cuts at: 509 509
Size: 3881
AlwNI CAG_nnn'CTG
Cuts at: 1186 2753 1186
Size: 1567 2314
ApaLI G'TGCA_C
Cuts at: 2651 2651
Size: 3881
ApoI r'AATT_y
Cuts at: 394 595 640 730 885 3234 3490 394
Size: 201 45 90 155 2349 256 785
Fragments arranged by size:
2349 785 256 201 155 90 45
AseI AT'TA_AT
Cuts at: 20 20
Size: 3881
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 3881
AvaI C'yCGr_G
Cuts at: 901 901
Size: 3881
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 835 868 835
Size: 33 3848
BamHI G'GATC_C
Cuts at: 906 906
Size: 3881
BanI G'GyrC_C
Cuts at: 897 1311 1559 1594 897
Size: 414 248 35 3184
Fragments arranged by size:
3184 414 248 35
BanII G_rGCy'C
Cuts at: 895 1925 895
Size: 1030 2851
BbeI G_GCGC'C
Cuts at: 1315 1563 1315
Size: 248 3633
BciVI GTATCCnnnnn_n'
Cuts at: 1779 2540 3881 1779
Size: 761 1341 1779
Fragments arranged by size:
1779 1341 761
BclI T'GATC_A
Cuts at: 182 1401 182
Size: 1219 2662
BglII A'GATC_T
Cuts at: 1396 3433 1396
Size: 2037 1844
BlpI GC'TnA_GC
Cuts at: 789 993 789
Size: 204 3677
Bme1580I G_kGCm'C
Cuts at: 1506 1599 2655 1506
Size: 93 1056 2732
Fragments arranged by size:
2732 1056 93
BmrI ACTGGGnnnn_n'
Cuts at: 1093 1174 1509 3653 1093
Size: 81 335 2144 1321
Fragments arranged by size:
2144 1321 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2248 2248
Size: 3881
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 400 401 1057 1936 2428 2726 400
Size: 1 656 879 492 298 1555
Fragments arranged by size:
1555 879 656 492 298 1
BsaI GGTCTCn'nnnn_
Cuts at: 3585 3585
Size: 3881
BsaAI yAC'GTr
Cuts at: 616 1234 616
Size: 618 3263
BsaBI GATnn'nnATC
Cuts at: 6 143 1395 3432 6
Size: 137 1252 2037 455
Fragments arranged by size:
2037 1252 455 137
BsaHI Gr'CG_yC
Cuts at: 1312 1560 2262 3758 1312
Size: 248 702 1496 1435
Fragments arranged by size:
1496 1435 702 248
BsaWI w'CCGG_w
Cuts at: 1070 1591 2269 2543 2690 3101 1070
Size: 521 678 274 147 411 1850
Fragments arranged by size:
1850 678 521 411 274 147
BseYI C'CCAG_C
Cuts at: 1306 2064 2641 3137 1306
Size: 758 577 496 2050
Fragments arranged by size:
2050 758 577 496
BsiEI CG_ry'CG
Cuts at: 104 1423 1469 2677 104
Size: 1319 46 1208 1308
Fragments arranged by size:
1319 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 895 1673 1863 2655 895
Size: 778 190 792 2121
Fragments arranged by size:
2121 792 778 190
BsmI GAATG_Cn'
Cuts at: 211 211
Size: 3881
Bsp1286I G_dGCh'C
Cuts at: 895 1506 1599 1673 1863 1925 2655 895
Size: 611 93 74 190 62 730 2121
Fragments arranged by size:
2121 730 611 190 93 74 62
BspEI T'CCGG_A
Cuts at: 3101 3101
Size: 3881
BspHI T'CATG_A
Cuts at: 288 3057 288
Size: 2769 1112
BspMI ACCTGCnnnn'nnnn_
Cuts at: 166 931 1145 1447 1828 166
Size: 765 214 302 381 2219
Fragments arranged by size:
2219 765 381 302 214
BsrBI CCG'CTC
Cuts at: 50 2173 2227 3711 3876 50
Size: 2123 54 1484 165 55
Fragments arranged by size:
2123 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 264 702 1793 264
Size: 438 1091 2352
Fragments arranged by size:
2352 1091 438
BsrFI r'CCGG_y
Cuts at: 1879 2060 2269 1879
Size: 181 209 3491
Fragments arranged by size:
3491 209 181
BssSI C'ACGA_G
Cuts at: 2152 2510 2152
Size: 358 3523
BstAPI GCAn_nnn'nTGC
Cuts at: 263 1258 263
Size: 995 2886
BstBI TT'CG_AA
Cuts at: 2242 2242
Size: 3881
BstYI r'GATC_y
Cuts at: 1 906 1373 1396 1731 1977 2989 3075 3087
Size: 905 467 23 335 246 1012 86 12
Cuts at: 3087 3433 1
Size: 346 449
Fragments arranged by size:
1012 905 467 449 346 335 246 86
23 12
BtgI C'CryG_G
Cuts at: 107 107
Size: 3881
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 1831 2012 2175 1831
Size: 181 163 3537
Fragments arranged by size:
3537 181 163
BtsI GCAGTG_nn'
Cuts at: 1124 1124
Size: 3881
DraI TTT'AAA
Cuts at: 653 880 653
Size: 227 3654
DraIII CAC_nnn'GTG
Cuts at: 132 132
Size: 3881
DrdI GACnn_nn'nnGTC
Cuts at: 1587 2445 1587
Size: 858 3023
EaeI y'GGCC_r
Cuts at: 1466 1640 2031 2058 3403 1466
Size: 174 391 27 1345 1944
Fragments arranged by size:
1944 1345 391 174 27
EagI C'GGCC_G
Cuts at: 1466 1466
Size: 3881
EarI CTCTTCn'nnn_
Cuts at: 1904 2114 1904
Size: 210 3671
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2089 2399 2545 3691 2089
Size: 310 146 1146 2279
Fragments arranged by size:
2279 1146 310 146
EcoICRI GAG'CTC
Cuts at: 893 893
Size: 3881
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1706 2138 2885 3153 1706
Size: 432 747 268 2434
Fragments arranged by size:
2434 747 432 268
EcoO109I rG'GnC_Cy
Cuts at: 1020 1020
Size: 3881
EcoRI G'AATT_C
Cuts at: 730 885 730
Size: 155 3726
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 1900 1900
Size: 3881
FspI TGC'GCA
Cuts at: 1662 1662
Size: 3881
HaeII r_GCGC'y
Cuts at: 1315 1563 2585 3564 1315
Size: 248 1022 979 1632
Fragments arranged by size:
1632 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 373 405 1723 1753 1755 1785 373
Size: 32 1318 30 2 30 2469
Fragments arranged by size:
2469 1318 32 30 30 2
HincII GTy'rAC
Cuts at: 920 920
Size: 3881
KasI G'GCGC_C
Cuts at: 1311 1559 1311
Size: 248 3633
KpnI G_GTAC'C
Cuts at: 901 901
Size: 3881
MluI A'CGCG_T
Cuts at: 3314 3314
Size: 3881
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 299 662 2552 2736 299
Size: 363 1890 184 1444
Fragments arranged by size:
1890 1444 363 184
MscI TGG'CCA
Cuts at: 1642 1642
Size: 3881
MslI CAynn'nnrTG
Cuts at: 509 739 1870 1997 509
Size: 230 1131 127 2393
Fragments arranged by size:
2393 1131 230 127
MspA1I CmG'CkG
Cuts at: 992 1306 1666 2679 2924 992
Size: 314 360 1013 245 1949
Fragments arranged by size:
1949 1013 360 314 245
NaeI GCC'GGC
Cuts at: 2062 2062
Size: 3881
NarI GG'CG_CC
Cuts at: 1312 1560 1312
Size: 248 3633
NcoI C'CATG_G
Cuts at: 107 107
Size: 3881
NgoMIV G'CCGG_C
Cuts at: 2060 2060
Size: 3881
NspI r_CATG'y
Cuts at: 934 1434 2341 934
Size: 500 907 2474
Fragments arranged by size:
2474 907 500
PciI A'CATG_T
Cuts at: 2337 2337
Size: 3881
PflMI CCAn_nnn'nTGG
Cuts at: 274 274
Size: 3881
PmeI GTTT'AAAC
Cuts at: 880 880
Size: 3881
PmlI CAC'GTG
Cuts at: 616 616
Size: 3881
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3020 3052 3020
Size: 32 3849
PstI C_TGCA'G
Cuts at: 851 928 1613 851
Size: 77 685 3119
Fragments arranged by size:
3119 685 77
PvuI CG_AT'CG
Cuts at: 104 104
Size: 3881
PvuII CAG'CTG
Cuts at: 1306 1666 1306
Size: 360 3521
RsrII CG'GwC_CG
Cuts at: 2076 2076
Size: 3881
SacI G_AGCT'C
Cuts at: 895 895
Size: 3881
SalI G'TCGA_C
Cuts at: 918 918
Size: 3881
SapI GCTCTTCn'nnn_
Cuts at: 1904 2114 1904
Size: 210 3671
SbfI CC_TGCA'GG
Cuts at: 928 928
Size: 3881
ScaI AGT'ACT
Cuts at: 3398 3398
Size: 3881
SfcI C'TryA_G
Cuts at: 33 847 924 1609 2602 2793 33
Size: 814 77 685 993 191 1121
Fragments arranged by size:
1121 993 814 685 191 77
SfoI GGC'GCC
Cuts at: 1313 1561 1313
Size: 248 3633
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 3881
SmaI CCC'GGG
Cuts at: 903 903
Size: 3881
SmlI C'TyrA_G
Cuts at: 379 416 1036 1951 2443 2705 379
Size: 37 620 915 492 262 1555
Fragments arranged by size:
1555 915 620 492 262 37
SpeI A'CTAG_T
Cuts at: 67 67
Size: 3881
SphI G_CATG'C
Cuts at: 934 1434 934
Size: 500 3381
StyI C'CwwG_G
Cuts at: 107 268 1015 1369 107
Size: 161 747 354 2619
Fragments arranged by size:
2619 747 354 161
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1464 1597 2056 2265 1464
Size: 133 459 209 3080
Fragments arranged by size:
3080 459 209 133
TatI w'GTAC_w
Cuts at: 3396 3396
Size: 3881
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 676 708 3398 3430 676
Size: 32 2690 32 1127
Fragments arranged by size:
2690 1127 32 32
Tth111I GACn'n_nGTC
Cuts at: 1678 1678
Size: 3881
XbaI T'CTAG_A
Cuts at: 534 912 534
Size: 378 3503
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1376 1376
Size: 3881
XmaI C'CCGG_G
Cuts at: 901 901
Size: 3881
XmnI GAAnn'nnTTC
Cuts at: 595 770 595
Size: 175 3706
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AgeI AleI AlwNI
ApaLI ApoI AseI AsiSI AvaI BaeI BamHI BanI
BanII BbeI BciVI BclI BglII BlpI Bme1580I BmrI
BplI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BseYI
BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI
BsrDI BsrFI BssSI BstAPI BstBI BstYI BtgI BtgZI
BtsI DraI DraIII DrdI EaeI EagI EarI EciI
EcoICRI Eco57MI EcoO109I EcoRI FalI FspI HaeII Hin4I
HincII KasI KpnI MluI MmeI MscI MslI MspA1I
NaeI NarI NcoI NgoMIV NspI PciI PflMI PmeI
PmlI PpiI PstI PvuI PvuII RsrII SacI SalI
SapI SbfI ScaI SfcI SfoI SgfI SmaI SmlI
SpeI SphI StyI TaqII TatI TstI Tth111I XbaI
XcmI XmaI XmnI
Enzymes that do not cut:
AarI AatII AfeI AflII AhdI AlfI AloI ApaI
AscI AvrII BbsI BbvCI BcgI BfrBI BglI BmgBI
BmtI BpmI Bpu10I BsaXI BseRI BsgI BsiWI BsmBI
BsrGI BssHII BstEII BstXI BstZ17I Bsu36I ClaI CspCI
EcoNI EcoRV FseI FspAI HindIII HpaI MfeI NdeI
NheI NotI NruI NsiI PacI PasI PfoI PpuMI
PshAI PsiI PspOMI PspXI PsrI SacII SanDI SexAI
SfiI SgrAI SnaBI SrfI SspI StuI SwaI XhoI
ZraI