Restriction map for
pF1KE0173
(Circular) (Six-base) MAPSORT of: pF1KE0173.seq Check: 52 from: 1 to: 4007
pF1KE0173 4007 bp
With 182 enzymes: SgfI *
April 24, 2009 13:11 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 534 534
Size: 4007
AatII G_ACGT'C
Cuts at: 155 155
Size: 4007
AccI GT'mk_AC
Cuts at: 1045 1045
Size: 4007
Acc65I G'GTAC_C
Cuts at: 1023 1023
Size: 4007
AclI AA'CG_TT
Cuts at: 3847 3847
Size: 4007
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 888 1832 2264 3011 3279 888
Size: 944 432 747 268 1616
Fragments arranged by size:
1616 944 747 432 268
AflIII A'CryG_T
Cuts at: 233 1359 2463 3440 233
Size: 1126 1104 977 800
Fragments arranged by size:
1126 1104 977 800
AgeI A'CCGG_T
Cuts at: 2395 2395
Size: 4007
AlwNI CAG_nnn'CTG
Cuts at: 126 1312 2879 126
Size: 1186 1567 1254
Fragments arranged by size:
1567 1254 1186
ApaI G_GGCC'C
Cuts at: 811 811
Size: 4007
ApaLI G'TGCA_C
Cuts at: 2777 2777
Size: 4007
ApoI r'AATT_y
Cuts at: 1011 3360 3616 1011
Size: 2349 256 1402
Fragments arranged by size:
2349 1402 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 4007
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4007
AvaI C'yCGr_G
Cuts at: 1027 1027
Size: 4007
BamHI G'GATC_C
Cuts at: 512 1032 512
Size: 520 3487
BanI G'GyrC_C
Cuts at: 793 950 1023 1437 1685 1720 793
Size: 157 73 414 248 35 3080
Fragments arranged by size:
3080 414 248 157 73 35
BanII G_rGCy'C
Cuts at: 273 292 644 811 1021 2051 273
Size: 19 352 167 210 1030 2229
Fragments arranged by size:
2229 1030 352 210 167 19
BbeI G_GCGC'C
Cuts at: 797 1441 1689 797
Size: 644 248 3115
Fragments arranged by size:
3115 644 248
BbvCI CC'TCA_GC
Cuts at: 785 785
Size: 4007
BciVI GTATCCnnnnn_n'
Cuts at: 855 1905 2666 4007 855
Size: 1050 761 1341 855
Fragments arranged by size:
1341 1050 855 761
BclI T'GATC_A
Cuts at: 1527 1527
Size: 4007
BglI GCCn_nnn'nGGC
Cuts at: 815 983 815
Size: 168 3839
BglII A'GATC_T
Cuts at: 1522 3559 1522
Size: 2037 1970
BlpI GC'TnA_GC
Cuts at: 1119 1119
Size: 4007
Bme1580I G_kGCm'C
Cuts at: 811 1632 1725 2781 811
Size: 821 93 1056 2037
Fragments arranged by size:
2037 1056 821 93
BmgBI CAC'GTC
Cuts at: 669 669
Size: 4007
BmrI ACTGGGnnnn_n'
Cuts at: 495 877 1219 1300 1635 3779 495
Size: 382 342 81 335 2144 723
Fragments arranged by size:
2144 723 382 342 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 658 2374 658
Size: 1716 2291
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 575 896 575
Size: 321 3686
Bpu10I CC'TnA_GC
Cuts at: 785 785
Size: 4007
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 750 1183 2062 2554 2852 750
Size: 433 879 492 298 1905
Fragments arranged by size:
1905 879 492 433 298
BsaI GGTCTCn'nnnn_
Cuts at: 3711 3711
Size: 4007
BsaAI yAC'GTr
Cuts at: 1360 1360
Size: 4007
BsaBI GATnn'nnATC
Cuts at: 6 1521 3558 6
Size: 1515 2037 455
Fragments arranged by size:
2037 1515 455
BsaHI Gr'CG_yC
Cuts at: 152 794 800 1438 1686 2388 3884 152
Size: 642 6 638 248 702 1496 275
Fragments arranged by size:
1496 702 642 638 275 248 6
BsaWI w'CCGG_w
Cuts at: 1196 1717 2395 2669 2816 3227 1196
Size: 521 678 274 147 411 1976
Fragments arranged by size:
1976 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 184 214 656 686 184
Size: 30 442 30 3505
Fragments arranged by size:
3505 442 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 652 802 936 652
Size: 150 134 3723
Fragments arranged by size:
3723 150 134
BseYI C'CCAG_C
Cuts at: 1432 2190 2767 3263 1432
Size: 758 577 496 2176
Fragments arranged by size:
2176 758 577 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 231 949 231
Size: 718 3289
BsiEI CG_ry'CG
Cuts at: 104 1549 1595 2803 104
Size: 1445 46 1208 1308
Fragments arranged by size:
1445 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 644 1021 1799 1989 2781 644
Size: 377 778 190 792 1870
Fragments arranged by size:
1870 792 778 377 190
BsmBI CGTCTCn'nnnn_
Cuts at: 659 659
Size: 4007
Bsp1286I G_dGCh'C
Cuts at: 273 292 644 811 1021 1632 1725 1799 1989
Size: 19 352 167 210 611 93 74 190
Cuts at: 1989 2051 2781 273
Size: 62 730 1499
Fragments arranged by size:
1499 730 611 352 210 190 167 93
74 62 19
BspEI T'CCGG_A
Cuts at: 3227 3227
Size: 4007
BspHI T'CATG_A
Cuts at: 3183 3183
Size: 4007
BspMI ACCTGCnnnn'nnnn_
Cuts at: 534 1057 1271 1573 1954 534
Size: 523 214 302 381 2587
Fragments arranged by size:
2587 523 381 302 214
BsrBI CCG'CTC
Cuts at: 50 2299 2353 3837 4002 50
Size: 2249 54 1484 165 55
Fragments arranged by size:
2249 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 1919 1919
Size: 4007
BsrFI r'CCGG_y
Cuts at: 961 2005 2186 2395 961
Size: 1044 181 209 2573
Fragments arranged by size:
2573 1044 209 181
BssSI C'ACGA_G
Cuts at: 632 2278 2636 632
Size: 1646 358 2003
Fragments arranged by size:
2003 1646 358
BstAPI GCAn_nnn'nTGC
Cuts at: 1384 1384
Size: 4007
BstBI TT'CG_AA
Cuts at: 2368 2368
Size: 4007
BstXI CCAn_nnnn'nTGG
Cuts at: 655 702 655
Size: 47 3960
BstYI r'GATC_y
Cuts at: 1 122 512 602 1032 1499 1522 1857 2103
Size: 121 390 90 430 467 23 335 246
Cuts at: 2103 3115 3201 3213 3559 1
Size: 1012 86 12 346 449
Fragments arranged by size:
1012 467 449 430 390 346 335 246
121 90 86 23 12
Bsu36I CC'TnA_GG
Cuts at: 179 179
Size: 4007
BtgI C'CryG_G
Cuts at: 107 398 614 107
Size: 291 216 3500
Fragments arranged by size:
3500 291 216
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 378 1957 2138 2301 378
Size: 1579 181 163 2084
Fragments arranged by size:
2084 1579 181 163
BtsI GCAGTG_nn'
Cuts at: 1250 1250
Size: 4007
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 954 989 954
Size: 35 3972
DraI TTT'AAA
Cuts at: 1006 1006
Size: 4007
DrdI GACnn_nn'nnGTC
Cuts at: 1713 2571 1713
Size: 858 3149
EaeI y'GGCC_r
Cuts at: 303 692 959 1592 1766 2157 2184 3529 303
Size: 389 267 633 174 391 27 1345 781
Fragments arranged by size:
1345 781 633 391 389 267 174 27
EagI C'GGCC_G
Cuts at: 1592 1592
Size: 4007
EarI CTCTTCn'nnn_
Cuts at: 107 649 2030 2240 107
Size: 542 1381 210 1874
Fragments arranged by size:
1874 1381 542 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 325 2215 2525 2671 3817 325
Size: 1890 310 146 1146 515
Fragments arranged by size:
1890 1146 515 310 146
EcoICRI GAG'CTC
Cuts at: 642 1019 642
Size: 377 3630
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 575 888 896 1832 2264 3011 3279 575
Size: 313 8 936 432 747 268 1303
Fragments arranged by size:
1303 936 747 432 313 268 8
EcoNI CCTnn'n_nnAGG
Cuts at: 913 913
Size: 4007
EcoO109I rG'GnC_Cy
Cuts at: 808 1146 808
Size: 338 3669
EcoRI G'AATT_C
Cuts at: 1011 1011
Size: 4007
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2026 2026
Size: 4007
FspI TGC'GCA
Cuts at: 1788 1788
Size: 4007
HaeII r_GCGC'y
Cuts at: 797 1441 1689 2711 3690 797
Size: 644 248 1022 979 1114
Fragments arranged by size:
1114 1022 979 644 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1849 1879 1881 1911 1849
Size: 30 2 30 3945
Fragments arranged by size:
3945 30 30 2
HincII GTy'rAC
Cuts at: 1046 1046
Size: 4007
KasI G'GCGC_C
Cuts at: 793 1437 1685 793
Size: 644 248 3115
Fragments arranged by size:
3115 644 248
KpnI G_GTAC'C
Cuts at: 1027 1027
Size: 4007
MluI A'CGCG_T
Cuts at: 233 3440 233
Size: 3207 800
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 263 2678 2862 263
Size: 2415 184 1408
Fragments arranged by size:
2415 1408 184
MscI TGG'CCA
Cuts at: 305 694 1768 305
Size: 389 1074 2544
Fragments arranged by size:
2544 1074 389
MslI CAynn'nnrTG
Cuts at: 833 1996 2123 833
Size: 1163 127 2717
Fragments arranged by size:
2717 1163 127
MspA1I CmG'CkG
Cuts at: 561 651 1118 1432 1792 2805 3050 561
Size: 90 467 314 360 1013 245 1518
Fragments arranged by size:
1518 1013 467 360 314 245 90
NaeI GCC'GGC
Cuts at: 963 2188 963
Size: 1225 2782
NarI GG'CG_CC
Cuts at: 794 1438 1686 794
Size: 644 248 3115
Fragments arranged by size:
3115 644 248
NcoI C'CATG_G
Cuts at: 107 107
Size: 4007
NgoMIV G'CCGG_C
Cuts at: 961 2186 961
Size: 1225 2782
NspI r_CATG'y
Cuts at: 1060 1560 2467 1060
Size: 500 907 2600
Fragments arranged by size:
2600 907 500
PasI CC'CwG_GG
Cuts at: 804 915 804
Size: 111 3896
PciI A'CATG_T
Cuts at: 2463 2463
Size: 4007
PfoI T'CCnGG_A
Cuts at: 323 323
Size: 4007
PmeI GTTT'AAAC
Cuts at: 1006 1006
Size: 4007
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3146 3178 3146
Size: 32 3975
PspOMI G'GGCC_C
Cuts at: 807 807
Size: 4007
PstI C_TGCA'G
Cuts at: 560 983 1054 1739 560
Size: 423 71 685 2828
Fragments arranged by size:
2828 685 423 71
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4007
PvuII CAG'CTG
Cuts at: 651 1432 1792 651
Size: 781 360 2866
Fragments arranged by size:
2866 781 360
RsrII CG'GwC_CG
Cuts at: 2202 2202
Size: 4007
SacI G_AGCT'C
Cuts at: 644 1021 644
Size: 377 3630
SalI G'TCGA_C
Cuts at: 1044 1044
Size: 4007
SapI GCTCTTCn'nnn_
Cuts at: 649 2030 2240 649
Size: 1381 210 2416
Fragments arranged by size:
2416 1381 210
SbfI CC_TGCA'GG
Cuts at: 983 1054 983
Size: 71 3936
ScaI AGT'ACT
Cuts at: 3524 3524
Size: 4007
SfcI C'TryA_G
Cuts at: 33 473 556 979 1050 1735 2728 2919 33
Size: 440 83 423 71 685 993 191 1121
Fragments arranged by size:
1121 993 685 440 423 191 83 71
SfoI GGC'GCC
Cuts at: 795 1439 1687 795
Size: 644 248 3115
Fragments arranged by size:
3115 644 248
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4007
SmaI CCC'GGG
Cuts at: 1029 1029
Size: 4007
SmlI C'TyrA_G
Cuts at: 729 1162 2077 2569 2831 729
Size: 433 915 492 262 1905
Fragments arranged by size:
1905 915 492 433 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4007
SphI G_CATG'C
Cuts at: 1060 1560 1060
Size: 500 3507
StyI C'CwwG_G
Cuts at: 107 1141 1495 107
Size: 1034 354 2619
Fragments arranged by size:
2619 1034 354
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1590 1723 2182 2391 1590
Size: 133 459 209 3206
Fragments arranged by size:
3206 459 209 133
TatI w'GTAC_w
Cuts at: 3522 3522
Size: 4007
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 184 216 656 688 3524 3556 184
Size: 32 440 32 2836 32 635
Fragments arranged by size:
2836 635 440 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1804 1804
Size: 4007
XbaI T'CTAG_A
Cuts at: 1038 1038
Size: 4007
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1502 1502
Size: 4007
XmaI C'CCGG_G
Cuts at: 1027 1027
Size: 4007
ZraI GAC'GTC
Cuts at: 153 153
Size: 4007
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflIII AgeI
AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI
BanI BanII BbeI BbvCI BciVI BclI BglI BglII
BlpI Bme1580I BmgBI BmrI BplI BpmI Bpu10I BpuEI
BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI
BsgI BsiEI BsiHKAI BsmBI Bsp1286I BspEI BspHI BspMI
BsrBI BsrDI BsrFI BssSI BstAPI BstBI BstXI BstYI
Bsu36I BtgI BtgZI BtsI CspCI DraI DrdI EaeI
EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI
FalI FspI HaeII Hin4I HincII KasI KpnI MluI
MmeI MscI MslI MspA1I NaeI NarI NcoI NgoMIV
NspI PasI PciI PfoI PmeI PpiI PspOMI PstI
PvuI PvuII RsrII SacI SalI SapI SbfI ScaI
SfcI SfoI SgfI SmaI SmlI SpeI SphI StyI
TaqII TatI TstI Tth111I XbaI XcmI XmaI ZraI
Enzymes that do not cut:
AfeI AflII AhdI AleI AlfI AloI AscI AvrII
BaeI BbsI BcgI BfrBI BmtI BsiWI BsmI BsrGI
BssHII BstEII BstZ17I ClaI DraIII EcoRV FseI FspAI
HindIII HpaI MfeI NdeI NheI NotI NruI NsiI
PacI PflMI PmlI PpuMI PshAI PsiI PspXI PsrI
SacII SanDI SexAI SfiI SgrAI SnaBI SrfI SspI
StuI SwaI XhoI XmnI