Restriction map for
pF1KE0169
(Circular) (Six-base) MAPSORT of: pF1KE0169.seq Check: 1864 from: 1 to: 3692
pF1KE0169 3692 bp
With 182 enzymes: SgfI *
April 24, 2009 13:11 ..
AccI GT'mk_AC
Cuts at: 730 730
Size: 3692
Acc65I G'GTAC_C
Cuts at: 708 708
Size: 3692
AclI AA'CG_TT
Cuts at: 3532 3532
Size: 3692
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 405 545 1517 1949 2696 2964 405
Size: 140 972 432 747 268 1133
Fragments arranged by size:
1133 972 747 432 268 140
AflIII A'CryG_T
Cuts at: 1044 2148 3125 1044
Size: 1104 977 1611
Fragments arranged by size:
1611 1104 977
AgeI A'CCGG_T
Cuts at: 2080 2080
Size: 3692
AlwNI CAG_nnn'CTG
Cuts at: 408 997 2564 408
Size: 589 1567 1536
Fragments arranged by size:
1567 1536 589
ApaLI G'TGCA_C
Cuts at: 2462 2462
Size: 3692
ApoI r'AATT_y
Cuts at: 295 358 696 3045 3301 295
Size: 63 338 2349 256 686
Fragments arranged by size:
2349 686 338 256 63
AseI AT'TA_AT
Cuts at: 20 20
Size: 3692
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 3692
AvaI C'yCGr_G
Cuts at: 666 712 666
Size: 46 3646
BamHI G'GATC_C
Cuts at: 717 717
Size: 3692
BanI G'GyrC_C
Cuts at: 708 1122 1370 1405 708
Size: 414 248 35 2995
Fragments arranged by size:
2995 414 248 35
BanII G_rGCy'C
Cuts at: 706 1736 706
Size: 1030 2662
BbeI G_GCGC'C
Cuts at: 1126 1374 1126
Size: 248 3444
BbsI GAAGACnn'nnnn_
Cuts at: 303 303
Size: 3692
BciVI GTATCCnnnnn_n'
Cuts at: 1590 2351 3692 1590
Size: 761 1341 1590
Fragments arranged by size:
1590 1341 761
BclI T'GATC_A
Cuts at: 1212 1212
Size: 3692
BglII A'GATC_T
Cuts at: 560 1207 3244 560
Size: 647 2037 1008
Fragments arranged by size:
2037 1008 647
BlpI GC'TnA_GC
Cuts at: 804 804
Size: 3692
Bme1580I G_kGCm'C
Cuts at: 1317 1410 2466 1317
Size: 93 1056 2543
Fragments arranged by size:
2543 1056 93
BmrI ACTGGGnnnn_n'
Cuts at: 218 904 985 1320 3464 218
Size: 686 81 335 2144 446
Fragments arranged by size:
2144 686 446 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2059 2059
Size: 3692
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 418 418
Size: 3692
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 231 393 868 1747 2239 2537 231
Size: 162 475 879 492 298 1386
Fragments arranged by size:
1386 879 492 475 298 162
BsaI GGTCTCn'nnnn_
Cuts at: 3396 3396
Size: 3692
BsaAI yAC'GTr
Cuts at: 1045 1045
Size: 3692
BsaBI GATnn'nnATC
Cuts at: 6 1206 3243 6
Size: 1200 2037 455
Fragments arranged by size:
2037 1200 455
BsaHI Gr'CG_yC
Cuts at: 1123 1371 2073 3569 1123
Size: 248 702 1496 1246
Fragments arranged by size:
1496 1246 702 248
BsaWI w'CCGG_w
Cuts at: 881 1402 2080 2354 2501 2912 881
Size: 521 678 274 147 411 1661
Fragments arranged by size:
1661 678 521 411 274 147
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 635 635
Size: 3692
BseYI C'CCAG_C
Cuts at: 1117 1875 2452 2948 1117
Size: 758 577 496 1861
Fragments arranged by size:
1861 758 577 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 502 502
Size: 3692
BsiEI CG_ry'CG
Cuts at: 104 1234 1280 2488 104
Size: 1130 46 1208 1308
Fragments arranged by size:
1308 1208 1130 46
BsiHKAI G_wGCw'C
Cuts at: 706 1484 1674 2466 706
Size: 778 190 792 1932
Fragments arranged by size:
1932 792 778 190
BsmBI CGTCTCn'nnnn_
Cuts at: 581 581
Size: 3692
Bsp1286I G_dGCh'C
Cuts at: 706 1317 1410 1484 1674 1736 2466 706
Size: 611 93 74 190 62 730 1932
Fragments arranged by size:
1932 730 611 190 93 74 62
BspEI T'CCGG_A
Cuts at: 2912 2912
Size: 3692
BspHI T'CATG_A
Cuts at: 2868 2868
Size: 3692
BspMI ACCTGCnnnn'nnnn_
Cuts at: 742 956 1258 1639 742
Size: 214 302 381 2795
Fragments arranged by size:
2795 381 302 214
BsrBI CCG'CTC
Cuts at: 50 1984 2038 3522 3687 50
Size: 1934 54 1484 165 55
Fragments arranged by size:
1934 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 1604 1604
Size: 3692
BsrFI r'CCGG_y
Cuts at: 676 1690 1871 2080 676
Size: 1014 181 209 2288
Fragments arranged by size:
2288 1014 209 181
BssSI C'ACGA_G
Cuts at: 1963 2321 1963
Size: 358 3334
BstAPI GCAn_nnn'nTGC
Cuts at: 153 1069 153
Size: 916 2776
BstBI TT'CG_AA
Cuts at: 2053 2053
Size: 3692
BstYI r'GATC_y
Cuts at: 1 185 560 717 1184 1207 1542 1788 2800
Size: 184 375 157 467 23 335 246 1012
Cuts at: 2800 2886 2898 3244 1
Size: 86 12 346 449
Fragments arranged by size:
1012 467 449 375 346 335 246 184
157 86 23 12
BtgI C'CryG_G
Cuts at: 107 602 107
Size: 495 3197
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 351 1642 1823 1986 351
Size: 1291 181 163 2057
Fragments arranged by size:
2057 1291 181 163
BtsI GCAGTG_nn'
Cuts at: 664 678 935 664
Size: 14 257 3421
Fragments arranged by size:
3421 257 14
DraI TTT'AAA
Cuts at: 691 691
Size: 3692
DrdI GACnn_nn'nnGTC
Cuts at: 1398 2256 1398
Size: 858 2834
EaeI y'GGCC_r
Cuts at: 678 1277 1451 1842 1869 3214 678
Size: 599 174 391 27 1345 1156
Fragments arranged by size:
1345 1156 599 391 174 27
EagI C'GGCC_G
Cuts at: 1277 1277
Size: 3692
EarI CTCTTCn'nnn_
Cuts at: 239 602 1715 1925 239
Size: 363 1113 210 2006
Fragments arranged by size:
2006 1113 363 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 556 1900 2210 2356 3502 556
Size: 1344 310 146 1146 746
Fragments arranged by size:
1344 1146 746 310 146
EcoICRI GAG'CTC
Cuts at: 704 704
Size: 3692
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 405 418 545 1517 1949 2696 2964 405
Size: 13 127 972 432 747 268 1133
Fragments arranged by size:
1133 972 747 432 268 127 13
EcoO109I rG'GnC_Cy
Cuts at: 831 831
Size: 3692
EcoRI G'AATT_C
Cuts at: 358 696 358
Size: 338 3354
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 1711 1711
Size: 3692
FspI TGC'GCA
Cuts at: 1473 1473
Size: 3692
HaeII r_GCGC'y
Cuts at: 1126 1374 2396 3375 1126
Size: 248 1022 979 1443
Fragments arranged by size:
1443 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 347 379 1534 1564 1566 1596 347
Size: 32 1155 30 2 30 2443
Fragments arranged by size:
2443 1155 32 30 30 2
HincII GTy'rAC
Cuts at: 536 731 536
Size: 195 3497
KasI G'GCGC_C
Cuts at: 1122 1370 1122
Size: 248 3444
KpnI G_GTAC'C
Cuts at: 712 712
Size: 3692
MluI A'CGCG_T
Cuts at: 3125 3125
Size: 3692
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 2363 2547 2363
Size: 184 3508
MscI TGG'CCA
Cuts at: 1453 1453
Size: 3692
MslI CAynn'nnrTG
Cuts at: 1681 1808 1681
Size: 127 3565
MspA1I CmG'CkG
Cuts at: 803 1117 1477 2490 2735 803
Size: 314 360 1013 245 1760
Fragments arranged by size:
1760 1013 360 314 245
NaeI GCC'GGC
Cuts at: 678 1873 678
Size: 1195 2497
NarI GG'CG_CC
Cuts at: 1123 1371 1123
Size: 248 3444
NcoI C'CATG_G
Cuts at: 107 602 107
Size: 495 3197
NgoMIV G'CCGG_C
Cuts at: 676 1871 676
Size: 1195 2497
NspI r_CATG'y
Cuts at: 310 429 745 1245 2152 310
Size: 119 316 500 907 1850
Fragments arranged by size:
1850 907 500 316 119
PciI A'CATG_T
Cuts at: 2148 2148
Size: 3692
PfoI T'CCnGG_A
Cuts at: 188 188
Size: 3692
PmeI GTTT'AAAC
Cuts at: 691 691
Size: 3692
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2831 2863 2831
Size: 32 3660
PshAI GACnn'nnGTC
Cuts at: 234 234
Size: 3692
PstI C_TGCA'G
Cuts at: 659 739 1424 659
Size: 80 685 2927
Fragments arranged by size:
2927 685 80
PvuI CG_AT'CG
Cuts at: 104 104
Size: 3692
PvuII CAG'CTG
Cuts at: 1117 1477 1117
Size: 360 3332
RsrII CG'GwC_CG
Cuts at: 1887 1887
Size: 3692
SacI G_AGCT'C
Cuts at: 706 706
Size: 3692
SalI G'TCGA_C
Cuts at: 729 729
Size: 3692
SapI GCTCTTCn'nnn_
Cuts at: 1715 1925 1715
Size: 210 3482
SbfI CC_TGCA'GG
Cuts at: 739 739
Size: 3692
ScaI AGT'ACT
Cuts at: 3209 3209
Size: 3692
SfcI C'TryA_G
Cuts at: 33 655 735 1420 2413 2604 33
Size: 622 80 685 993 191 1121
Fragments arranged by size:
1121 993 685 622 191 80
SfoI GGC'GCC
Cuts at: 1124 1372 1124
Size: 248 3444
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 3692
SmaI CCC'GGG
Cuts at: 714 714
Size: 3692
SmlI C'TyrA_G
Cuts at: 210 372 847 1762 2254 2516 210
Size: 162 475 915 492 262 1386
Fragments arranged by size:
1386 915 492 475 262 162
SpeI A'CTAG_T
Cuts at: 67 67
Size: 3692
SphI G_CATG'C
Cuts at: 745 1245 745
Size: 500 3192
StyI C'CwwG_G
Cuts at: 107 602 826 1180 107
Size: 495 224 354 2619
Fragments arranged by size:
2619 495 354 224
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1275 1408 1867 2076 1275
Size: 133 459 209 2891
Fragments arranged by size:
2891 459 209 133
TatI w'GTAC_w
Cuts at: 3207 3207
Size: 3692
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3209 3241 3209
Size: 32 3660
Tth111I GACn'n_nGTC
Cuts at: 1489 1489
Size: 3692
XbaI T'CTAG_A
Cuts at: 723 723
Size: 3692
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1187 1187
Size: 3692
XmaI C'CCGG_G
Cuts at: 712 712
Size: 3692
XmnI GAAnn'nnTTC
Cuts at: 271 271
Size: 3692
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AgeI AlwNI ApaLI
ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI
BbsI BciVI BclI BglII BlpI Bme1580I BmrI BplI
BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BseRI
BseYI BsgI BsiEI BsiHKAI BsmBI Bsp1286I BspEI BspHI
BspMI BsrBI BsrDI BsrFI BssSI BstAPI BstBI BstYI
BtgI BtgZI BtsI DraI DrdI EaeI EagI EarI
EciI EcoICRI Eco57MI EcoO109I EcoRI FalI FspI HaeII
Hin4I HincII KasI KpnI MluI MmeI MscI MslI
MspA1I NaeI NarI NcoI NgoMIV NspI PciI PfoI
PmeI PpiI PshAI PstI PvuI PvuII RsrII SacI
SalI SapI SbfI ScaI SfcI SfoI SgfI SmaI
SmlI SpeI SphI StyI TaqII TatI TstI Tth111I
XbaI XcmI XmaI XmnI
Enzymes that do not cut:
AarI AatII AfeI AflII AhdI AleI AlfI AloI
ApaI AscI AvrII BaeI BbvCI BcgI BfrBI BglI
BmgBI BmtI Bpu10I BsaXI BsiWI BsmI BsrGI BssHII
BstEII BstXI BstZ17I Bsu36I ClaI CspCI DraIII EcoNI
EcoRV FseI FspAI HindIII HpaI MfeI NdeI NheI
NotI NruI NsiI PacI PasI PflMI PmlI PpuMI
PsiI PspOMI PspXI PsrI SacII SanDI SexAI SfiI
SgrAI SnaBI SrfI SspI StuI SwaI XhoI ZraI