Restriction map for
pF1KE0167
(Circular) (Six-base) MAPSORT of: pF1KE0167.seq Check: 9250 from: 1 to: 3605
pF1KE0167 3605 bp
With 182 enzymes: SgfI *
April 24, 2009 13:11 ..
AccI GT'mk_AC
Cuts at: 643 643
Size: 3605
Acc65I G'GTAC_C
Cuts at: 227 621 227
Size: 394 3211
AclI AA'CG_TT
Cuts at: 3445 3445
Size: 3605
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1430 1862 2609 2877 1430
Size: 432 747 268 2158
Fragments arranged by size:
2158 747 432 268
AflIII A'CryG_T
Cuts at: 389 957 2061 3038 389
Size: 568 1104 977 956
Fragments arranged by size:
1104 977 956 568
AgeI A'CCGG_T
Cuts at: 1993 1993
Size: 3605
AlwNI CAG_nnn'CTG
Cuts at: 910 2477 910
Size: 1567 2038
ApaLI G'TGCA_C
Cuts at: 511 2375 511
Size: 1864 1741
ApoI r'AATT_y
Cuts at: 609 2958 3214 609
Size: 2349 256 1000
Fragments arranged by size:
2349 1000 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 3605
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 3605
AvaI C'yCGr_G
Cuts at: 145 268 625 145
Size: 123 357 3125
Fragments arranged by size:
3125 357 123
BamHI G'GATC_C
Cuts at: 630 630
Size: 3605
BanI G'GyrC_C
Cuts at: 227 621 1035 1283 1318 227
Size: 394 414 248 35 2514
Fragments arranged by size:
2514 414 394 248 35
BanII G_rGCy'C
Cuts at: 269 619 1649 269
Size: 350 1030 2225
Fragments arranged by size:
2225 1030 350
BbeI G_GCGC'C
Cuts at: 1039 1287 1039
Size: 248 3357
BbsI GAAGACnn'nnnn_
Cuts at: 345 345
Size: 3605
BciVI GTATCCnnnnn_n'
Cuts at: 1503 2264 3605 1503
Size: 761 1341 1503
Fragments arranged by size:
1503 1341 761
BclI T'GATC_A
Cuts at: 1125 1125
Size: 3605
BglII A'GATC_T
Cuts at: 1120 3157 1120
Size: 2037 1568
BlpI GC'TnA_GC
Cuts at: 717 717
Size: 3605
Bme1580I G_kGCm'C
Cuts at: 515 1230 1323 2379 515
Size: 715 93 1056 1741
Fragments arranged by size:
1741 1056 715 93
BmrI ACTGGGnnnn_n'
Cuts at: 817 898 1233 3377 817
Size: 81 335 2144 1045
Fragments arranged by size:
2144 1045 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 1972 1972
Size: 3605
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 439 478 547 439
Size: 39 69 3497
Fragments arranged by size:
3497 69 39
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 781 1660 2152 2450 781
Size: 879 492 298 1936
Fragments arranged by size:
1936 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 3309 3309
Size: 3605
BsaAI yAC'GTr
Cuts at: 390 958 390
Size: 568 3037
BsaBI GATnn'nnATC
Cuts at: 6 593 1119 3156 6
Size: 587 526 2037 455
Fragments arranged by size:
2037 587 526 455
BsaHI Gr'CG_yC
Cuts at: 350 1036 1284 1986 3482 350
Size: 686 248 702 1496 473
Fragments arranged by size:
1496 702 686 473 248
BsaWI w'CCGG_w
Cuts at: 182 794 1315 1993 2267 2414 2825 182
Size: 612 521 678 274 147 411 962
Fragments arranged by size:
962 678 612 521 411 274 147
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 286 289 286
Size: 3 3602
BseYI C'CCAG_C
Cuts at: 540 1030 1788 2365 2861 540
Size: 490 758 577 496 1284
Fragments arranged by size:
1284 758 577 496 490
BsiEI CG_ry'CG
Cuts at: 104 1147 1193 2401 104
Size: 1043 46 1208 1308
Fragments arranged by size:
1308 1208 1043 46
BsiHKAI G_wGCw'C
Cuts at: 515 619 1397 1587 2379 515
Size: 104 778 190 792 1741
Fragments arranged by size:
1741 792 778 190 104
BsiWI C'GTAC_G
Cuts at: 386 386
Size: 3605
Bsp1286I G_dGCh'C
Cuts at: 269 515 619 1230 1323 1397 1587 1649 2379
Size: 246 104 611 93 74 190 62 730
Cuts at: 2379 269
Size: 1495
Fragments arranged by size:
1495 730 611 246 190 104 93 74
62
BspEI T'CCGG_A
Cuts at: 182 2825 182
Size: 2643 962
BspHI T'CATG_A
Cuts at: 2781 2781
Size: 3605
BspMI ACCTGCnnnn'nnnn_
Cuts at: 655 869 1171 1552 655
Size: 214 302 381 2708
Fragments arranged by size:
2708 381 302 214
BsrBI CCG'CTC
Cuts at: 50 1897 1951 3435 3600 50
Size: 1847 54 1484 165 55
Fragments arranged by size:
1847 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 1517 1517
Size: 3605
BsrFI r'CCGG_y
Cuts at: 224 338 1603 1784 1993 224
Size: 114 1265 181 209 1836
Fragments arranged by size:
1836 1265 209 181 114
BssSI C'ACGA_G
Cuts at: 1876 2234 1876
Size: 358 3247
BstAPI GCAn_nnn'nTGC
Cuts at: 982 982
Size: 3605
BstBI TT'CG_AA
Cuts at: 356 1966 356
Size: 1610 1995
BstYI r'GATC_y
Cuts at: 1 630 1097 1120 1455 1701 2713 2799 2811
Size: 629 467 23 335 246 1012 86 12
Cuts at: 2811 3157 1
Size: 346 449
Fragments arranged by size:
1012 629 467 449 346 335 246 86
23 12
BtgI C'CryG_G
Cuts at: 569 569
Size: 3605
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 1555 1736 1899 1555
Size: 181 163 3261
Fragments arranged by size:
3261 181 163
BtsI GCAGTG_nn'
Cuts at: 848 848
Size: 3605
DraI TTT'AAA
Cuts at: 604 604
Size: 3605
DrdI GACnn_nn'nnGTC
Cuts at: 1311 2169 1311
Size: 858 2747
EaeI y'GGCC_r
Cuts at: 1190 1364 1755 1782 3127 1190
Size: 174 391 27 1345 1668
Fragments arranged by size:
1668 1345 391 174 27
EagI C'GGCC_G
Cuts at: 1190 1190
Size: 3605
EarI CTCTTCn'nnn_
Cuts at: 1628 1838 1628
Size: 210 3395
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 1813 2123 2269 3415 1813
Size: 310 146 1146 2003
Fragments arranged by size:
2003 1146 310 146
EcoICRI GAG'CTC
Cuts at: 617 617
Size: 3605
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 439 478 547 1430 1862 2609 2877 439
Size: 39 69 883 432 747 268 1167
Fragments arranged by size:
1167 883 747 432 268 69 39
EcoO109I rG'GnC_Cy
Cuts at: 204 744 204
Size: 540 3065
EcoRI G'AATT_C
Cuts at: 609 609
Size: 3605
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 1624 1624
Size: 3605
FspI TGC'GCA
Cuts at: 1386 1386
Size: 3605
HaeII r_GCGC'y
Cuts at: 1039 1287 2309 3288 1039
Size: 248 1022 979 1356
Fragments arranged by size:
1356 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1447 1477 1479 1509 1447
Size: 30 2 30 3543
Fragments arranged by size:
3543 30 30 2
HincII GTy'rAC
Cuts at: 644 644
Size: 3605
KasI G'GCGC_C
Cuts at: 1035 1283 1035
Size: 248 3357
KpnI G_GTAC'C
Cuts at: 231 625 231
Size: 394 3211
MluI A'CGCG_T
Cuts at: 3038 3038
Size: 3605
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 2276 2460 2276
Size: 184 3421
MscI TGG'CCA
Cuts at: 1366 1366
Size: 3605
MslI CAynn'nnrTG
Cuts at: 1594 1721 1594
Size: 127 3478
MspA1I CmG'CkG
Cuts at: 239 716 1030 1390 2403 2648 239
Size: 477 314 360 1013 245 1196
Fragments arranged by size:
1196 1013 477 360 314 245
NaeI GCC'GGC
Cuts at: 340 1786 340
Size: 1446 2159
NarI GG'CG_CC
Cuts at: 1036 1284 1036
Size: 248 3357
NcoI C'CATG_G
Cuts at: 569 569
Size: 3605
NgoMIV G'CCGG_C
Cuts at: 338 1784 338
Size: 1446 2159
NspI r_CATG'y
Cuts at: 658 1158 2065 658
Size: 500 907 2198
Fragments arranged by size:
2198 907 500
PciI A'CATG_T
Cuts at: 2061 2061
Size: 3605
PflMI CCAn_nnn'nTGG
Cuts at: 559 559
Size: 3605
PmeI GTTT'AAAC
Cuts at: 604 604
Size: 3605
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2744 2776 2744
Size: 32 3573
PpuMI rG'GwC_Cy
Cuts at: 204 204
Size: 3605
PstI C_TGCA'G
Cuts at: 652 1337 652
Size: 685 2920
PvuI CG_AT'CG
Cuts at: 104 104
Size: 3605
PvuII CAG'CTG
Cuts at: 1030 1390 1030
Size: 360 3245
RsrII CG'GwC_CG
Cuts at: 234 294 1800 234
Size: 60 1506 2039
Fragments arranged by size:
2039 1506 60
SacI G_AGCT'C
Cuts at: 619 619
Size: 3605
SalI G'TCGA_C
Cuts at: 642 642
Size: 3605
SapI GCTCTTCn'nnn_
Cuts at: 1628 1838 1628
Size: 210 3395
SbfI CC_TGCA'GG
Cuts at: 652 652
Size: 3605
ScaI AGT'ACT
Cuts at: 3122 3122
Size: 3605
SfcI C'TryA_G
Cuts at: 33 648 1333 2326 2517 33
Size: 615 685 993 191 1121
Fragments arranged by size:
1121 993 685 615 191
SfoI GGC'GCC
Cuts at: 1037 1285 1037
Size: 248 3357
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 3605
SmaI CCC'GGG
Cuts at: 627 627
Size: 3605
SmlI C'TyrA_G
Cuts at: 760 1675 2167 2429 760
Size: 915 492 262 1936
Fragments arranged by size:
1936 915 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 3605
SphI G_CATG'C
Cuts at: 658 1158 658
Size: 500 3105
StyI C'CwwG_G
Cuts at: 569 739 1093 569
Size: 170 354 3081
Fragments arranged by size:
3081 354 170
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1188 1321 1780 1989 1188
Size: 133 459 209 2804
Fragments arranged by size:
2804 459 209 133
TatI w'GTAC_w
Cuts at: 396 3120 396
Size: 2724 881
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3122 3154 3122
Size: 32 3573
Tth111I GACn'n_nGTC
Cuts at: 202 1402 202
Size: 1200 2405
XbaI T'CTAG_A
Cuts at: 636 636
Size: 3605
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1100 1100
Size: 3605
XmaI C'CCGG_G
Cuts at: 625 625
Size: 3605
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AgeI AlwNI ApaLI
ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI
BbsI BciVI BclI BglII BlpI Bme1580I BmrI BplI
BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BseRI
BseYI BsiEI BsiHKAI BsiWI Bsp1286I BspEI BspHI BspMI
BsrBI BsrDI BsrFI BssSI BstAPI BstBI BstYI BtgI
BtgZI BtsI DraI DrdI EaeI EagI EarI EciI
EcoICRI Eco57MI EcoO109I EcoRI FalI FspI HaeII Hin4I
HincII KasI KpnI MluI MmeI MscI MslI MspA1I
NaeI NarI NcoI NgoMIV NspI PciI PflMI PmeI
PpiI PpuMI PstI PvuI PvuII RsrII SacI SalI
SapI SbfI ScaI SfcI SfoI SgfI SmaI SmlI
SpeI SphI StyI TaqII TatI TstI Tth111I XbaI
XcmI XmaI
Enzymes that do not cut:
AarI AatII AfeI AflII AhdI AleI AlfI AloI
ApaI AscI AvrII BaeI BbvCI BcgI BfrBI BglI
BmgBI BmtI Bpu10I BsaXI BsgI BsmI BsmBI BsrGI
BssHII BstEII BstXI BstZ17I Bsu36I ClaI CspCI DraIII
EcoNI EcoRV FseI FspAI HindIII HpaI MfeI NdeI
NheI NotI NruI NsiI PacI PasI PfoI PmlI
PshAI PsiI PspOMI PspXI PsrI SacII SanDI SexAI
SfiI SgrAI SnaBI SrfI SspI StuI SwaI XhoI
XmnI ZraI