Restriction map for
pF1KE0161
(Circular) (Six-base) MAPSORT of: pF1KE0161.seq Check: 2789 from: 1 to: 3908
pF1KE0161 3908 bp
With 182 enzymes: SgfI *
April 24, 2009 13:11 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 295 295
Size: 3908
AatII G_ACGT'C
Cuts at: 533 533
Size: 3908
AccI GT'mk_AC
Cuts at: 946 946
Size: 3908
Acc65I G'GTAC_C
Cuts at: 924 924
Size: 3908
AclI AA'CG_TT
Cuts at: 3748 3748
Size: 3908
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 323 1733 2165 2912 3180 323
Size: 1410 432 747 268 1051
Fragments arranged by size:
1410 1051 747 432 268
AflIII A'CryG_T
Cuts at: 1260 2364 3341 1260
Size: 1104 977 1827
Fragments arranged by size:
1827 1104 977
AgeI A'CCGG_T
Cuts at: 2296 2296
Size: 3908
AhdI GACnn_n'nnGTC
Cuts at: 333 333
Size: 3908
AleI CACnn'nnGTG
Cuts at: 643 643
Size: 3908
AlwNI CAG_nnn'CTG
Cuts at: 312 1213 2780 312
Size: 901 1567 1440
Fragments arranged by size:
1567 1440 901
ApaI G_GGCC'C
Cuts at: 448 448
Size: 3908
ApaLI G'TGCA_C
Cuts at: 2678 2678
Size: 3908
ApoI r'AATT_y
Cuts at: 912 3261 3517 912
Size: 2349 256 1303
Fragments arranged by size:
2349 1303 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 3908
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 3908
AvaI C'yCGr_G
Cuts at: 928 928
Size: 3908
BamHI G'GATC_C
Cuts at: 546 933 546
Size: 387 3521
BanI G'GyrC_C
Cuts at: 279 924 1338 1586 1621 279
Size: 645 414 248 35 2566
Fragments arranged by size:
2566 645 414 248 35
BanII G_rGCy'C
Cuts at: 448 922 1952 448
Size: 474 1030 2404
Fragments arranged by size:
2404 1030 474
BbeI G_GCGC'C
Cuts at: 1342 1590 1342
Size: 248 3660
BbsI GAAGACnn'nnnn_
Cuts at: 329 360 329
Size: 31 3877
BbvCI CC'TCA_GC
Cuts at: 223 223
Size: 3908
BciVI GTATCCnnnnn_n'
Cuts at: 1806 2567 3908 1806
Size: 761 1341 1806
Fragments arranged by size:
1806 1341 761
BclI T'GATC_A
Cuts at: 1428 1428
Size: 3908
BfrBI ATG'CAT
Cuts at: 602 602
Size: 3908
BglII A'GATC_T
Cuts at: 1423 3460 1423
Size: 2037 1871
BlpI GC'TnA_GC
Cuts at: 1020 1020
Size: 3908
Bme1580I G_kGCm'C
Cuts at: 284 448 1533 1626 2682 284
Size: 164 1085 93 1056 1510
Fragments arranged by size:
1510 1085 1056 164 93
BmrI ACTGGGnnnn_n'
Cuts at: 1120 1201 1536 3680 1120
Size: 81 335 2144 1348
Fragments arranged by size:
2144 1348 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 777 2275 777
Size: 1498 2410
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 194 747 194
Size: 553 3355
Bpu10I CC'TnA_GC
Cuts at: 223 223
Size: 3908
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1084 1963 2455 2753 1084
Size: 879 492 298 2239
Fragments arranged by size:
2239 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 700 3612 700
Size: 2912 996
BsaAI yAC'GTr
Cuts at: 1261 1261
Size: 3908
BsaBI GATnn'nnATC
Cuts at: 6 1422 3459 6
Size: 1416 2037 455
Fragments arranged by size:
2037 1416 455
BsaHI Gr'CG_yC
Cuts at: 530 1339 1587 2289 3785 530
Size: 809 248 702 1496 653
Fragments arranged by size:
1496 809 702 653 248
BsaWI w'CCGG_w
Cuts at: 1097 1618 2296 2570 2717 3128 1097
Size: 521 678 274 147 411 1877
Fragments arranged by size:
1877 678 521 411 274 147
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 321 321
Size: 3908
BseYI C'CCAG_C
Cuts at: 1333 2091 2668 3164 1333
Size: 758 577 496 2077
Fragments arranged by size:
2077 758 577 496
BsiEI CG_ry'CG
Cuts at: 104 1450 1496 2704 104
Size: 1346 46 1208 1308
Fragments arranged by size:
1346 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 922 1700 1890 2682 922
Size: 778 190 792 2148
Fragments arranged by size:
2148 792 778 190
BsmI GAATG_Cn'
Cuts at: 717 717
Size: 3908
Bsp1286I G_dGCh'C
Cuts at: 284 448 922 1533 1626 1700 1890 1952 2682
Size: 164 474 611 93 74 190 62 730
Cuts at: 2682 284
Size: 1510
Fragments arranged by size:
1510 730 611 474 190 164 93 74
62
BspEI T'CCGG_A
Cuts at: 3128 3128
Size: 3908
BspHI T'CATG_A
Cuts at: 3084 3084
Size: 3908
BspMI ACCTGCnnnn'nnnn_
Cuts at: 295 958 1172 1474 1855 295
Size: 663 214 302 381 2348
Fragments arranged by size:
2348 663 381 302 214
BsrBI CCG'CTC
Cuts at: 50 2200 2254 3738 3903 50
Size: 2150 54 1484 165 55
Fragments arranged by size:
2150 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 1820 1820
Size: 3908
BsrFI r'CCGG_y
Cuts at: 709 1906 2087 2296 709
Size: 1197 181 209 2321
Fragments arranged by size:
2321 1197 209 181
BssSI C'ACGA_G
Cuts at: 2179 2537 2179
Size: 358 3550
BstAPI GCAn_nnn'nTGC
Cuts at: 145 1285 145
Size: 1140 2768
BstBI TT'CG_AA
Cuts at: 2269 2269
Size: 3908
BstXI CCAn_nnnn'nTGG
Cuts at: 114 114
Size: 3908
BstYI r'GATC_y
Cuts at: 1 546 744 933 1400 1423 1758 2004 3016
Size: 545 198 189 467 23 335 246 1012
Cuts at: 3016 3102 3114 3460 1
Size: 86 12 346 449
Fragments arranged by size:
1012 545 467 449 346 335 246 198
189 86 23 12
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 1858 2039 2202 1858
Size: 181 163 3564
Fragments arranged by size:
3564 181 163
BtsI GCAGTG_nn'
Cuts at: 819 1151 819
Size: 332 3576
DraI TTT'AAA
Cuts at: 907 907
Size: 3908
DraIII CAC_nnn'GTG
Cuts at: 267 267
Size: 3908
DrdI GACnn_nn'nnGTC
Cuts at: 463 1614 2472 463
Size: 1151 858 1899
Fragments arranged by size:
1899 1151 858
EaeI y'GGCC_r
Cuts at: 389 861 1493 1667 2058 2085 3430 389
Size: 472 632 174 391 27 1345 867
Fragments arranged by size:
1345 867 632 472 391 174 27
EagI C'GGCC_G
Cuts at: 1493 1493
Size: 3908
EarI CTCTTCn'nnn_
Cuts at: 1931 2141 1931
Size: 210 3698
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2116 2426 2572 3718 2116
Size: 310 146 1146 2306
Fragments arranged by size:
2306 1146 310 146
EcoICRI GAG'CTC
Cuts at: 920 920
Size: 3908
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 194 323 747 1733 2165 2912 3180 194
Size: 129 424 986 432 747 268 922
Fragments arranged by size:
986 922 747 432 424 268 129
EcoO109I rG'GnC_Cy
Cuts at: 444 1047 444
Size: 603 3305
EcoRI G'AATT_C
Cuts at: 912 912
Size: 3908
EcoRV GAT'ATC
Cuts at: 736 736
Size: 3908
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 1927 1927
Size: 3908
FspI TGC'GCA
Cuts at: 1689 1689
Size: 3908
HaeII r_GCGC'y
Cuts at: 1342 1590 2612 3591 1342
Size: 248 1022 979 1659
Fragments arranged by size:
1659 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 111 143 319 351 1750 1780 1782 1812 111
Size: 32 176 32 1399 30 2 30 2207
Fragments arranged by size:
2207 1399 176 32 32 30 30 2
HincII GTy'rAC
Cuts at: 204 947 204
Size: 743 3165
HindIII A'AGCT_T
Cuts at: 630 630
Size: 3908
KasI G'GCGC_C
Cuts at: 1338 1586 1338
Size: 248 3660
KpnI G_GTAC'C
Cuts at: 928 928
Size: 3908
MluI A'CGCG_T
Cuts at: 3341 3341
Size: 3908
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 617 2579 2763 617
Size: 1962 184 1762
Fragments arranged by size:
1962 1762 184
MscI TGG'CCA
Cuts at: 863 1669 863
Size: 806 3102
MslI CAynn'nnrTG
Cuts at: 112 643 1897 2024 112
Size: 531 1254 127 1996
Fragments arranged by size:
1996 1254 531 127
MspA1I CmG'CkG
Cuts at: 1019 1333 1693 2706 2951 1019
Size: 314 360 1013 245 1976
Fragments arranged by size:
1976 1013 360 314 245
NaeI GCC'GGC
Cuts at: 2089 2089
Size: 3908
NarI GG'CG_CC
Cuts at: 1339 1587 1339
Size: 248 3660
NdeI CA'TA_TG
Cuts at: 688 688
Size: 3908
NgoMIV G'CCGG_C
Cuts at: 2087 2087
Size: 3908
NsiI A_TGCA'T
Cuts at: 604 604
Size: 3908
NspI r_CATG'y
Cuts at: 161 961 1461 2368 161
Size: 800 500 907 1701
Fragments arranged by size:
1701 907 800 500
PciI A'CATG_T
Cuts at: 2364 2364
Size: 3908
PmeI GTTT'AAAC
Cuts at: 907 907
Size: 3908
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3047 3079 3047
Size: 32 3876
PspOMI G'GGCC_C
Cuts at: 444 444
Size: 3908
PstI C_TGCA'G
Cuts at: 955 1640 955
Size: 685 3223
PvuI CG_AT'CG
Cuts at: 104 104
Size: 3908
PvuII CAG'CTG
Cuts at: 1333 1693 1333
Size: 360 3548
RsrII CG'GwC_CG
Cuts at: 2103 2103
Size: 3908
SacI G_AGCT'C
Cuts at: 922 922
Size: 3908
SalI G'TCGA_C
Cuts at: 945 945
Size: 3908
SapI GCTCTTCn'nnn_
Cuts at: 1931 2141 1931
Size: 210 3698
SbfI CC_TGCA'GG
Cuts at: 955 955
Size: 3908
ScaI AGT'ACT
Cuts at: 3425 3425
Size: 3908
SexAI A'CCwGG_T
Cuts at: 263 263
Size: 3908
SfcI C'TryA_G
Cuts at: 33 470 951 1636 2629 2820 33
Size: 437 481 685 993 191 1121
Fragments arranged by size:
1121 993 685 481 437 191
SfoI GGC'GCC
Cuts at: 1340 1588 1340
Size: 248 3660
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 3908
SmaI CCC'GGG
Cuts at: 930 930
Size: 3908
SmlI C'TyrA_G
Cuts at: 1063 1978 2470 2732 1063
Size: 915 492 262 2239
Fragments arranged by size:
2239 915 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 3908
SphI G_CATG'C
Cuts at: 961 1461 961
Size: 500 3408
StuI AGG'CCT
Cuts at: 179 179
Size: 3908
StyI C'CwwG_G
Cuts at: 551 1042 1396 551
Size: 491 354 3063
Fragments arranged by size:
3063 491 354
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1491 1624 2083 2292 1491
Size: 133 459 209 3107
Fragments arranged by size:
3107 459 209 133
TatI w'GTAC_w
Cuts at: 3423 3423
Size: 3908
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3425 3457 3425
Size: 32 3876
Tth111I GACn'n_nGTC
Cuts at: 199 1705 199
Size: 1506 2402
XbaI T'CTAG_A
Cuts at: 939 939
Size: 3908
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 600 799 871 1403 600
Size: 199 72 532 3105
Fragments arranged by size:
3105 532 199 72
XmaI C'CCGG_G
Cuts at: 928 928
Size: 3908
ZraI GAC'GTC
Cuts at: 531 531
Size: 3908
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflIII AgeI
AhdI AleI AlwNI ApaI ApaLI ApoI AseI AsiSI
AvaI BamHI BanI BanII BbeI BbsI BbvCI BciVI
BclI BfrBI BglII BlpI Bme1580I BmrI BplI BpmI
Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BseRI
BseYI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI
BsrBI BsrDI BsrFI BssSI BstAPI BstBI BstXI BstYI
BtgZI BtsI DraI DraIII DrdI EaeI EagI EarI
EciI EcoICRI Eco57MI EcoO109I EcoRI EcoRV FalI FspI
HaeII Hin4I HincII HindIII KasI KpnI MluI MmeI
MscI MslI MspA1I NaeI NarI NdeI NgoMIV NsiI
NspI PciI PmeI PpiI PspOMI PstI PvuI PvuII
RsrII SacI SalI SapI SbfI ScaI SexAI SfcI
SfoI SgfI SmaI SmlI SpeI SphI StuI StyI
TaqII TatI TstI Tth111I XbaI XcmI XmaI ZraI
Enzymes that do not cut:
AfeI AflII AlfI AloI AscI AvrII BaeI BcgI
BglI BmgBI BmtI BsaXI BsgI BsiWI BsmBI BsrGI
BssHII BstEII BstZ17I Bsu36I BtgI ClaI CspCI EcoNI
FseI FspAI HpaI MfeI NcoI NheI NotI NruI
PacI PasI PflMI PfoI PmlI PpuMI PshAI PsiI
PspXI PsrI SacII SanDI SfiI SgrAI SnaBI SrfI
SspI SwaI XhoI XmnI