Restriction map for
pF1KE0156
(Circular) (Six-base) MAPSORT of: pF1KE0156.seq Check: 9736 from: 1 to: 3896
pF1KE0156 3896 bp
With 182 enzymes: SgfI *
April 24, 2009 13:11 ..
AccI GT'mk_AC
Cuts at: 934 934
Size: 3896
Acc65I G'GTAC_C
Cuts at: 912 912
Size: 3896
AclI AA'CG_TT
Cuts at: 3736 3736
Size: 3896
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 854 1721 2153 2900 3168 854
Size: 867 432 747 268 1582
Fragments arranged by size:
1582 867 747 432 268
AflIII A'CryG_T
Cuts at: 332 449 1248 2352 3329 332
Size: 117 799 1104 977 899
Fragments arranged by size:
1104 977 899 799 117
AgeI A'CCGG_T
Cuts at: 2284 2284
Size: 3896
AlwNI CAG_nnn'CTG
Cuts at: 1201 2768 1201
Size: 1567 2329
ApaLI G'TGCA_C
Cuts at: 2666 2666
Size: 3896
ApoI r'AATT_y
Cuts at: 900 3249 3505 900
Size: 2349 256 1291
Fragments arranged by size:
2349 1291 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 3896
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 3896
AvaI C'yCGr_G
Cuts at: 302 659 916 302
Size: 357 257 3282
Fragments arranged by size:
3282 357 257
BamHI G'GATC_C
Cuts at: 680 921 680
Size: 241 3655
BanI G'GyrC_C
Cuts at: 356 496 912 1326 1574 1609 356
Size: 140 416 414 248 35 2643
Fragments arranged by size:
2643 416 414 248 140 35
BanII G_rGCy'C
Cuts at: 910 1940 910
Size: 1030 2866
BbeI G_GCGC'C
Cuts at: 1330 1578 1330
Size: 248 3648
BciVI GTATCCnnnnn_n'
Cuts at: 838 1794 2555 3896 838
Size: 956 761 1341 838
Fragments arranged by size:
1341 956 838 761
BclI T'GATC_A
Cuts at: 1416 1416
Size: 3896
BglI GCCn_nnn'nGGC
Cuts at: 325 325
Size: 3896
BglII A'GATC_T
Cuts at: 1411 3448 1411
Size: 2037 1859
BlpI GC'TnA_GC
Cuts at: 1008 1008
Size: 3896
Bme1580I G_kGCm'C
Cuts at: 499 1521 1614 2670 499
Size: 1022 93 1056 1725
Fragments arranged by size:
1725 1056 1022 93
BmgBI CAC'GTC
Cuts at: 333 333
Size: 3896
BmrI ACTGGGnnnn_n'
Cuts at: 474 1108 1189 1524 3668 474
Size: 634 81 335 2144 702
Fragments arranged by size:
2144 702 634 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2263 2263
Size: 3896
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 106 650 896 106
Size: 544 246 3106
Fragments arranged by size:
3106 544 246
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 475 1072 1951 2443 2741 475
Size: 597 879 492 298 1630
Fragments arranged by size:
1630 879 597 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 3600 3600
Size: 3896
BsaAI yAC'GTr
Cuts at: 1249 1249
Size: 3896
BsaBI GATnn'nnATC
Cuts at: 6 1410 3447 6
Size: 1404 2037 455
Fragments arranged by size:
2037 1404 455
BsaHI Gr'CG_yC
Cuts at: 112 1327 1575 2277 3773 112
Size: 1215 248 702 1496 235
Fragments arranged by size:
1496 1215 702 248 235
BsaWI w'CCGG_w
Cuts at: 1085 1606 2284 2558 2705 3116 1085
Size: 521 678 274 147 411 1865
Fragments arranged by size:
1865 678 521 411 274 147
BseYI C'CCAG_C
Cuts at: 270 1321 2079 2656 3152 270
Size: 1051 758 577 496 1014
Fragments arranged by size:
1051 1014 758 577 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 394 430 394
Size: 36 3860
BsiEI CG_ry'CG
Cuts at: 104 180 1438 1484 2692 104
Size: 76 1258 46 1208 1308
Fragments arranged by size:
1308 1258 1208 76 46
BsiHKAI G_wGCw'C
Cuts at: 910 1688 1878 2670 910
Size: 778 190 792 2136
Fragments arranged by size:
2136 792 778 190
BsmI GAATG_Cn'
Cuts at: 820 820
Size: 3896
BsmBI CGTCTCn'nnnn_
Cuts at: 119 250 119
Size: 131 3765
Bsp1286I G_dGCh'C
Cuts at: 499 910 1521 1614 1688 1878 1940 2670 499
Size: 411 611 93 74 190 62 730 1725
Fragments arranged by size:
1725 730 611 411 190 93 74 62
BspEI T'CCGG_A
Cuts at: 3116 3116
Size: 3896
BspHI T'CATG_A
Cuts at: 3072 3072
Size: 3896
BspMI ACCTGCnnnn'nnnn_
Cuts at: 946 1160 1462 1843 946
Size: 214 302 381 2999
Fragments arranged by size:
2999 381 302 214
BsrBI CCG'CTC
Cuts at: 50 137 170 184 2188 2242 3726 3891 50
Size: 87 33 14 2004 54 1484 165 55
Fragments arranged by size:
2004 1484 165 87 55 54 33 14
BsrDI GCAATG_nn'
Cuts at: 710 1808 710
Size: 1098 2798
BsrFI r'CCGG_y
Cuts at: 1894 2075 2284 1894
Size: 181 209 3506
Fragments arranged by size:
3506 209 181
BssSI C'ACGA_G
Cuts at: 2167 2525 2167
Size: 358 3538
BstAPI GCAn_nnn'nTGC
Cuts at: 1273 1273
Size: 3896
BstBI TT'CG_AA
Cuts at: 2257 2257
Size: 3896
BstYI r'GATC_y
Cuts at: 1 680 921 1388 1411 1746 1992 3004 3090
Size: 679 241 467 23 335 246 1012 86
Cuts at: 3090 3102 3448 1
Size: 12 346 449
Fragments arranged by size:
1012 679 467 449 346 335 246 241
86 23 12
Bsu36I CC'TnA_GG
Cuts at: 797 797
Size: 3896
BtgI C'CryG_G
Cuts at: 107 107
Size: 3896
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 1846 2027 2190 1846
Size: 181 163 3552
Fragments arranged by size:
3552 181 163
BtsI GCAGTG_nn'
Cuts at: 1139 1139
Size: 3896
DraI TTT'AAA
Cuts at: 895 895
Size: 3896
DrdI GACnn_nn'nnGTC
Cuts at: 1602 2460 1602
Size: 858 3038
EaeI y'GGCC_r
Cuts at: 177 200 1481 1655 2046 2073 3418 177
Size: 23 1281 174 391 27 1345 655
Fragments arranged by size:
1345 1281 655 391 174 27 23
EagI C'GGCC_G
Cuts at: 177 1481 177
Size: 1304 2592
EarI CTCTTCn'nnn_
Cuts at: 1919 2129 1919
Size: 210 3686
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2104 2414 2560 3706 2104
Size: 310 146 1146 2294
Fragments arranged by size:
2294 1146 310 146
EcoICRI GAG'CTC
Cuts at: 908 908
Size: 3896
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 106 650 854 896 1721 2153 2900 3168 106
Size: 544 204 42 825 432 747 268 834
Fragments arranged by size:
834 825 747 544 432 268 204 42
EcoO109I rG'GnC_Cy
Cuts at: 792 857 1035 792
Size: 65 178 3653
Fragments arranged by size:
3653 178 65
EcoRI G'AATT_C
Cuts at: 900 900
Size: 3896
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 516 1915 516
Size: 1399 2497
FspI TGC'GCA
Cuts at: 1677 1677
Size: 3896
HaeII r_GCGC'y
Cuts at: 1330 1578 2600 3579 1330
Size: 248 1022 979 1647
Fragments arranged by size:
1647 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 665 697 1738 1768 1770 1800 665
Size: 32 1041 30 2 30 2761
Fragments arranged by size:
2761 1041 32 30 30 2
HincII GTy'rAC
Cuts at: 486 935 486
Size: 449 3447
KasI G'GCGC_C
Cuts at: 1326 1574 1326
Size: 248 3648
KpnI G_GTAC'C
Cuts at: 916 916
Size: 3896
MluI A'CGCG_T
Cuts at: 3329 3329
Size: 3896
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 2567 2751 2567
Size: 184 3712
MscI TGG'CCA
Cuts at: 1657 1657
Size: 3896
MslI CAynn'nnrTG
Cuts at: 1885 2012 1885
Size: 127 3769
MspA1I CmG'CkG
Cuts at: 247 686 1007 1321 1681 2694 2939 247
Size: 439 321 314 360 1013 245 1204
Fragments arranged by size:
1204 1013 439 360 321 314 245
NaeI GCC'GGC
Cuts at: 2077 2077
Size: 3896
NarI GG'CG_CC
Cuts at: 1327 1575 1327
Size: 248 3648
NcoI C'CATG_G
Cuts at: 107 107
Size: 3896
NgoMIV G'CCGG_C
Cuts at: 2075 2075
Size: 3896
NspI r_CATG'y
Cuts at: 453 949 1449 2356 453
Size: 496 500 907 1993
Fragments arranged by size:
1993 907 500 496
PasI CC'CwG_GG
Cuts at: 703 703
Size: 3896
PciI A'CATG_T
Cuts at: 449 2352 449
Size: 1903 1993
PmeI GTTT'AAAC
Cuts at: 895 895
Size: 3896
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3035 3067 3035
Size: 32 3864
PpuMI rG'GwC_Cy
Cuts at: 792 857 792
Size: 65 3831
PstI C_TGCA'G
Cuts at: 522 943 1628 522
Size: 421 685 2790
Fragments arranged by size:
2790 685 421
PvuI CG_AT'CG
Cuts at: 104 104
Size: 3896
PvuII CAG'CTG
Cuts at: 1321 1681 1321
Size: 360 3536
RsrII CG'GwC_CG
Cuts at: 2091 2091
Size: 3896
SacI G_AGCT'C
Cuts at: 910 910
Size: 3896
SalI G'TCGA_C
Cuts at: 933 933
Size: 3896
SanDI GG'GwC_CC
Cuts at: 792 792
Size: 3896
SapI GCTCTTCn'nnn_
Cuts at: 1919 2129 1919
Size: 210 3686
SbfI CC_TGCA'GG
Cuts at: 943 943
Size: 3896
ScaI AGT'ACT
Cuts at: 3413 3413
Size: 3896
SfcI C'TryA_G
Cuts at: 33 518 939 1624 2617 2808 33
Size: 485 421 685 993 191 1121
Fragments arranged by size:
1121 993 685 485 421 191
SfoI GGC'GCC
Cuts at: 1328 1576 1328
Size: 248 3648
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 3896
SmaI CCC'GGG
Cuts at: 661 918 661
Size: 257 3639
SmlI C'TyrA_G
Cuts at: 490 1051 1966 2458 2720 490
Size: 561 915 492 262 1666
Fragments arranged by size:
1666 915 561 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 3896
SphI G_CATG'C
Cuts at: 949 1449 949
Size: 500 3396
StuI AGG'CCT
Cuts at: 379 379
Size: 3896
StyI C'CwwG_G
Cuts at: 107 149 623 1030 1384 107
Size: 42 474 407 354 2619
Fragments arranged by size:
2619 474 407 354 42
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 442 735 1479 1612 2071 2280 442
Size: 293 744 133 459 209 2058
Fragments arranged by size:
2058 744 459 293 209 133
TatI w'GTAC_w
Cuts at: 3411 3411
Size: 3896
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3413 3445 3413
Size: 32 3864
Tth111I GACn'n_nGTC
Cuts at: 472 1693 472
Size: 1221 2675
XbaI T'CTAG_A
Cuts at: 927 927
Size: 3896
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 360 1391 360
Size: 1031 2865
XmaI C'CCGG_G
Cuts at: 659 916 659
Size: 257 3639
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AgeI AlwNI ApaLI
ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI
BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI
BplI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI
BspHI BspMI BsrBI BsrDI BsrFI BssSI BstAPI BstBI
BstYI Bsu36I BtgI BtgZI BtsI DraI DrdI EaeI
EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI FalI
FspI HaeII Hin4I HincII KasI KpnI MluI MmeI
MscI MslI MspA1I NaeI NarI NcoI NgoMIV NspI
PasI PciI PmeI PpiI PpuMI PstI PvuI PvuII
RsrII SacI SalI SanDI SapI SbfI ScaI SfcI
SfoI SgfI SmaI SmlI SpeI SphI StuI StyI
TaqII TatI TstI Tth111I XbaI XcmI XmaI
Enzymes that do not cut:
AarI AatII AfeI AflII AhdI AleI AlfI AloI
ApaI AscI AvrII BaeI BbsI BbvCI BcgI BfrBI
BmtI Bpu10I BsaXI BseRI BsiWI BsrGI BssHII BstEII
BstXI BstZ17I ClaI CspCI DraIII EcoNI EcoRV FseI
FspAI HindIII HpaI MfeI NdeI NheI NotI NruI
NsiI PacI PflMI PfoI PmlI PshAI PsiI PspOMI
PspXI PsrI SacII SexAI SfiI SgrAI SnaBI SrfI
SspI SwaI XhoI XmnI ZraI