Restriction map for
pF1KE0152
(Circular) (Six-base) MAPSORT of: pF1KE0152.seq Check: 6803 from: 1 to: 3506
pF1KE0152 3506 bp
With 182 enzymes: SgfI *
April 24, 2009 13:11 ..
AccI GT'mk_AC
Cuts at: 544 544
Size: 3506
Acc65I G'GTAC_C
Cuts at: 522 522
Size: 3506
AclI AA'CG_TT
Cuts at: 3346 3346
Size: 3506
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1331 1763 2510 2778 1331
Size: 432 747 268 2059
Fragments arranged by size:
2059 747 432 268
AflIII A'CryG_T
Cuts at: 858 1962 2939 858
Size: 1104 977 1425
Fragments arranged by size:
1425 1104 977
AgeI A'CCGG_T
Cuts at: 1894 1894
Size: 3506
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 443 443
Size: 3506
AlwNI CAG_nnn'CTG
Cuts at: 165 811 2378 165
Size: 646 1567 1293
Fragments arranged by size:
1567 1293 646
ApaLI G'TGCA_C
Cuts at: 2276 2276
Size: 3506
ApoI r'AATT_y
Cuts at: 510 2859 3115 510
Size: 2349 256 901
Fragments arranged by size:
2349 901 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 3506
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 3506
AvaI C'yCGr_G
Cuts at: 433 526 433
Size: 93 3413
BamHI G'GATC_C
Cuts at: 531 531
Size: 3506
BanI G'GyrC_C
Cuts at: 522 936 1184 1219 522
Size: 414 248 35 2809
Fragments arranged by size:
2809 414 248 35
BanII G_rGCy'C
Cuts at: 520 1550 520
Size: 1030 2476
BbeI G_GCGC'C
Cuts at: 940 1188 940
Size: 248 3258
BciVI GTATCCnnnnn_n'
Cuts at: 1404 2165 3506 1404
Size: 761 1341 1404
Fragments arranged by size:
1404 1341 761
BclI T'GATC_A
Cuts at: 167 1026 167
Size: 859 2647
BglII A'GATC_T
Cuts at: 201 1021 3058 201
Size: 820 2037 649
Fragments arranged by size:
2037 820 649
BlpI GC'TnA_GC
Cuts at: 618 618
Size: 3506
Bme1580I G_kGCm'C
Cuts at: 1131 1224 2280 1131
Size: 93 1056 2357
Fragments arranged by size:
2357 1056 93
BmrI ACTGGGnnnn_n'
Cuts at: 718 799 1134 3278 718
Size: 81 335 2144 946
Fragments arranged by size:
2144 946 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 1873 1873
Size: 3506
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 415 682 1561 2053 2351 415
Size: 267 879 492 298 1570
Fragments arranged by size:
1570 879 492 298 267
BsaI GGTCTCn'nnnn_
Cuts at: 287 3210 287
Size: 2923 583
BsaAI yAC'GTr
Cuts at: 859 859
Size: 3506
BsaBI GATnn'nnATC
Cuts at: 6 137 1020 3057 6
Size: 131 883 2037 455
Fragments arranged by size:
2037 883 455 131
BsaHI Gr'CG_yC
Cuts at: 937 1185 1887 3383 937
Size: 248 702 1496 1060
Fragments arranged by size:
1496 1060 702 248
BsaWI w'CCGG_w
Cuts at: 695 1216 1894 2168 2315 2726 695
Size: 521 678 274 147 411 1475
Fragments arranged by size:
1475 678 521 411 274 147
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 236 236
Size: 3506
BseYI C'CCAG_C
Cuts at: 931 1689 2266 2762 931
Size: 758 577 496 1675
Fragments arranged by size:
1675 758 577 496
BsiEI CG_ry'CG
Cuts at: 104 1048 1094 2302 104
Size: 944 46 1208 1308
Fragments arranged by size:
1308 1208 944 46
BsiHKAI G_wGCw'C
Cuts at: 520 1298 1488 2280 520
Size: 778 190 792 1746
Fragments arranged by size:
1746 792 778 190
Bsp1286I G_dGCh'C
Cuts at: 520 1131 1224 1298 1488 1550 2280 520
Size: 611 93 74 190 62 730 1746
Fragments arranged by size:
1746 730 611 190 93 74 62
BspEI T'CCGG_A
Cuts at: 2726 2726
Size: 3506
BspHI T'CATG_A
Cuts at: 2682 2682
Size: 3506
BspMI ACCTGCnnnn'nnnn_
Cuts at: 556 770 1072 1453 556
Size: 214 302 381 2609
Fragments arranged by size:
2609 381 302 214
BsrBI CCG'CTC
Cuts at: 50 1798 1852 3336 3501 50
Size: 1748 54 1484 165 55
Fragments arranged by size:
1748 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 1418 1418
Size: 3506
BsrFI r'CCGG_y
Cuts at: 1504 1685 1894 1504
Size: 181 209 3116
Fragments arranged by size:
3116 209 181
BssSI C'ACGA_G
Cuts at: 1777 2135 1777
Size: 358 3148
BstAPI GCAn_nnn'nTGC
Cuts at: 408 883 408
Size: 475 3031
BstBI TT'CG_AA
Cuts at: 1867 1867
Size: 3506
BstYI r'GATC_y
Cuts at: 1 201 531 998 1021 1356 1602 2614 2700
Size: 200 330 467 23 335 246 1012 86
Cuts at: 2700 2712 3058 1
Size: 12 346 449
Fragments arranged by size:
1012 467 449 346 335 330 246 200
86 23 12
BtgI C'CryG_G
Cuts at: 107 107
Size: 3506
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 1456 1637 1800 1456
Size: 181 163 3162
Fragments arranged by size:
3162 181 163
BtsI GCAGTG_nn'
Cuts at: 749 749
Size: 3506
DraI TTT'AAA
Cuts at: 505 505
Size: 3506
DrdI GACnn_nn'nnGTC
Cuts at: 1212 2070 1212
Size: 858 2648
EaeI y'GGCC_r
Cuts at: 110 1091 1265 1656 1683 3028 110
Size: 981 174 391 27 1345 588
Fragments arranged by size:
1345 981 588 391 174 27
EagI C'GGCC_G
Cuts at: 1091 1091
Size: 3506
EarI CTCTTCn'nnn_
Cuts at: 410 1529 1739 410
Size: 1119 210 2177
Fragments arranged by size:
2177 1119 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 1714 2024 2170 3316 1714
Size: 310 146 1146 1904
Fragments arranged by size:
1904 1146 310 146
EcoICRI GAG'CTC
Cuts at: 518 518
Size: 3506
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1331 1763 2510 2778 1331
Size: 432 747 268 2059
Fragments arranged by size:
2059 747 432 268
EcoO109I rG'GnC_Cy
Cuts at: 645 645
Size: 3506
EcoRI G'AATT_C
Cuts at: 510 510
Size: 3506
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 1525 1525
Size: 3506
FspI TGC'GCA
Cuts at: 1287 1287
Size: 3506
HaeII r_GCGC'y
Cuts at: 940 1188 2210 3189 940
Size: 248 1022 979 1257
Fragments arranged by size:
1257 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1348 1378 1380 1410 1348
Size: 30 2 30 3444
Fragments arranged by size:
3444 30 30 2
HincII GTy'rAC
Cuts at: 545 545
Size: 3506
KasI G'GCGC_C
Cuts at: 936 1184 936
Size: 248 3258
KpnI G_GTAC'C
Cuts at: 526 526
Size: 3506
MluI A'CGCG_T
Cuts at: 2939 2939
Size: 3506
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 359 2177 2361 359
Size: 1818 184 1504
Fragments arranged by size:
1818 1504 184
MscI TGG'CCA
Cuts at: 1267 1267
Size: 3506
MslI CAynn'nnrTG
Cuts at: 1495 1622 1495
Size: 127 3379
MspA1I CmG'CkG
Cuts at: 617 931 1291 2304 2549 617
Size: 314 360 1013 245 1574
Fragments arranged by size:
1574 1013 360 314 245
NaeI GCC'GGC
Cuts at: 1687 1687
Size: 3506
NarI GG'CG_CC
Cuts at: 937 1185 937
Size: 248 3258
NcoI C'CATG_G
Cuts at: 107 107
Size: 3506
NgoMIV G'CCGG_C
Cuts at: 1685 1685
Size: 3506
NspI r_CATG'y
Cuts at: 559 1059 1966 559
Size: 500 907 2099
Fragments arranged by size:
2099 907 500
PciI A'CATG_T
Cuts at: 1962 1962
Size: 3506
PfoI T'CCnGG_A
Cuts at: 187 187
Size: 3506
PmeI GTTT'AAAC
Cuts at: 505 505
Size: 3506
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2645 2677 2645
Size: 32 3474
PstI C_TGCA'G
Cuts at: 553 1238 553
Size: 685 2821
PvuI CG_AT'CG
Cuts at: 104 104
Size: 3506
PvuII CAG'CTG
Cuts at: 931 1291 931
Size: 360 3146
RsrII CG'GwC_CG
Cuts at: 1701 1701
Size: 3506
SacI G_AGCT'C
Cuts at: 520 520
Size: 3506
SalI G'TCGA_C
Cuts at: 543 543
Size: 3506
SapI GCTCTTCn'nnn_
Cuts at: 410 1529 1739 410
Size: 1119 210 2177
Fragments arranged by size:
2177 1119 210
SbfI CC_TGCA'GG
Cuts at: 553 553
Size: 3506
ScaI AGT'ACT
Cuts at: 3023 3023
Size: 3506
SfcI C'TryA_G
Cuts at: 33 549 1234 2227 2418 33
Size: 516 685 993 191 1121
Fragments arranged by size:
1121 993 685 516 191
SfoI GGC'GCC
Cuts at: 938 1186 938
Size: 248 3258
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 3506
SmaI CCC'GGG
Cuts at: 528 528
Size: 3506
SmlI C'TyrA_G
Cuts at: 394 661 1576 2068 2330 394
Size: 267 915 492 262 1570
Fragments arranged by size:
1570 915 492 267 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 3506
SphI G_CATG'C
Cuts at: 559 1059 559
Size: 500 3006
StuI AGG'CCT
Cuts at: 156 156
Size: 3506
StyI C'CwwG_G
Cuts at: 107 640 994 107
Size: 533 354 2619
Fragments arranged by size:
2619 533 354
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1089 1222 1681 1890 1089
Size: 133 459 209 2705
Fragments arranged by size:
2705 459 209 133
TatI w'GTAC_w
Cuts at: 3021 3021
Size: 3506
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3023 3055 3023
Size: 32 3474
Tth111I GACn'n_nGTC
Cuts at: 1303 1303
Size: 3506
XbaI T'CTAG_A
Cuts at: 537 537
Size: 3506
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1001 1001
Size: 3506
XmaI C'CCGG_G
Cuts at: 526 526
Size: 3506
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AgeI AlfI AlwNI
ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII
BbeI BciVI BclI BglII BlpI Bme1580I BmrI BplI
BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BseRI BseYI
BsiEI BsiHKAI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI
BsrFI BssSI BstAPI BstBI BstYI BtgI BtgZI BtsI
DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI
EcoO109I EcoRI FalI FspI HaeII Hin4I HincII KasI
KpnI MluI MmeI MscI MslI MspA1I NaeI NarI
NcoI NgoMIV NspI PciI PfoI PmeI PpiI PstI
PvuI PvuII RsrII SacI SalI SapI SbfI ScaI
SfcI SfoI SgfI SmaI SmlI SpeI SphI StuI
StyI TaqII TatI TstI Tth111I XbaI XcmI XmaI
Enzymes that do not cut:
AarI AatII AfeI AflII AhdI AleI AloI ApaI
AscI AvrII BaeI BbsI BbvCI BcgI BfrBI BglI
BmgBI BmtI BpmI Bpu10I BsaXI BsgI BsiWI BsmI
BsmBI BsrGI BssHII BstEII BstXI BstZ17I Bsu36I ClaI
CspCI DraIII EcoNI EcoRV FseI FspAI HindIII HpaI
MfeI NdeI NheI NotI NruI NsiI PacI PasI
PflMI PmlI PpuMI PshAI PsiI PspOMI PspXI PsrI
SacII SanDI SexAI SfiI SgrAI SnaBI SrfI SspI
SwaI XhoI XmnI ZraI