Restriction map for
pF1KE0146
(Circular) (Six-base) MAPSORT of: pF1KE0146.seq Check: 5610 from: 1 to: 4001
pF1KE0146 4001 bp
With 182 enzymes: SgfI *
April 24, 2009 13:11 ..
AccI GT'mk_AC
Cuts at: 1039 1039
Size: 4001
Acc65I G'GTAC_C
Cuts at: 738 770 1017 738
Size: 32 247 3722
Fragments arranged by size:
3722 247 32
AclI AA'CG_TT
Cuts at: 3841 3841
Size: 4001
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 569 818 1826 2258 3005 3273 569
Size: 249 1008 432 747 268 1297
Fragments arranged by size:
1297 1008 747 432 268 249
AflII C'TTAA_G
Cuts at: 979 979
Size: 4001
AflIII A'CryG_T
Cuts at: 1353 2457 3434 1353
Size: 1104 977 1920
Fragments arranged by size:
1920 1104 977
AgeI A'CCGG_T
Cuts at: 2389 2389
Size: 4001
AlwNI CAG_nnn'CTG
Cuts at: 1306 2873 1306
Size: 1567 2434
ApaI G_GGCC'C
Cuts at: 138 144 138
Size: 6 3995
ApaLI G'TGCA_C
Cuts at: 2771 2771
Size: 4001
ApoI r'AATT_y
Cuts at: 790 1005 3354 3610 790
Size: 215 2349 256 1181
Fragments arranged by size:
2349 1181 256 215
AseI AT'TA_AT
Cuts at: 20 20
Size: 4001
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4001
AvaI C'yCGr_G
Cuts at: 137 1021 137
Size: 884 3117
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 397 430 397
Size: 33 3968
BamHI G'GATC_C
Cuts at: 780 888 1026 780
Size: 108 138 3755
Fragments arranged by size:
3755 138 108
BanI G'GyrC_C
Cuts at: 738 770 1017 1431 1679 1714 738
Size: 32 247 414 248 35 3025
Fragments arranged by size:
3025 414 248 247 35 32
BanII G_rGCy'C
Cuts at: 138 144 1015 2045 138
Size: 6 871 1030 2094
Fragments arranged by size:
2094 1030 871 6
BbeI G_GCGC'C
Cuts at: 1435 1683 1435
Size: 248 3753
BbsI GAAGACnn'nnnn_
Cuts at: 228 228
Size: 4001
BciVI GTATCCnnnnn_n'
Cuts at: 1899 2660 4001 1899
Size: 761 1341 1899
Fragments arranged by size:
1899 1341 761
BclI T'GATC_A
Cuts at: 1521 1521
Size: 4001
BglII A'GATC_T
Cuts at: 1516 3553 1516
Size: 2037 1964
BlpI GC'TnA_GC
Cuts at: 1113 1113
Size: 4001
Bme1580I G_kGCm'C
Cuts at: 138 144 1626 1719 2775 138
Size: 6 1482 93 1056 1364
Fragments arranged by size:
1482 1364 1056 93 6
BmrI ACTGGGnnnn_n'
Cuts at: 1213 1294 1629 3773 1213
Size: 81 335 2144 1441
Fragments arranged by size:
2144 1441 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2368 2368
Size: 4001
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 902 902
Size: 4001
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1177 2056 2548 2846 1177
Size: 879 492 298 2332
Fragments arranged by size:
2332 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 3705 3705
Size: 4001
BsaAI yAC'GTr
Cuts at: 1354 1354
Size: 4001
BsaBI GATnn'nnATC
Cuts at: 6 971 1515 3552 6
Size: 965 544 2037 455
Fragments arranged by size:
2037 965 544 455
BsaHI Gr'CG_yC
Cuts at: 378 1432 1680 2382 3878 378
Size: 1054 248 702 1496 501
Fragments arranged by size:
1496 1054 702 501 248
BsaWI w'CCGG_w
Cuts at: 213 1190 1711 2389 2663 2810 3221 213
Size: 977 521 678 274 147 411 993
Fragments arranged by size:
993 977 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 228 258 228
Size: 30 3971
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 547 547
Size: 4001
BseYI C'CCAG_C
Cuts at: 464 1426 2184 2761 3257 464
Size: 962 758 577 496 1208
Fragments arranged by size:
1208 962 758 577 496
BsiEI CG_ry'CG
Cuts at: 104 1543 1589 2797 104
Size: 1439 46 1208 1308
Fragments arranged by size:
1439 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 1015 1793 1983 2775 1015
Size: 778 190 792 2241
Fragments arranged by size:
2241 792 778 190
BsmI GAATG_Cn'
Cuts at: 722 722
Size: 4001
Bsp1286I G_dGCh'C
Cuts at: 138 144 1015 1626 1719 1793 1983 2045 2775
Size: 6 871 611 93 74 190 62 730
Cuts at: 2775 138
Size: 1364
Fragments arranged by size:
1364 871 730 611 190 93 74 62
6
BspEI T'CCGG_A
Cuts at: 3221 3221
Size: 4001
BspHI T'CATG_A
Cuts at: 3177 3177
Size: 4001
BspMI ACCTGCnnnn'nnnn_
Cuts at: 1051 1265 1567 1948 1051
Size: 214 302 381 3104
Fragments arranged by size:
3104 381 302 214
BsrBI CCG'CTC
Cuts at: 50 2293 2347 3831 3996 50
Size: 2243 54 1484 165 55
Fragments arranged by size:
2243 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 1913 1913
Size: 4001
BsrFI r'CCGG_y
Cuts at: 112 1999 2180 2389 112
Size: 1887 181 209 1724
Fragments arranged by size:
1887 1724 209 181
BssSI C'ACGA_G
Cuts at: 2272 2630 2272
Size: 358 3643
BstAPI GCAn_nnn'nTGC
Cuts at: 1378 1378
Size: 4001
BstBI TT'CG_AA
Cuts at: 2362 2362
Size: 4001
BstYI r'GATC_y
Cuts at: 1 780 888 1026 1493 1516 1851 2097 3109
Size: 779 108 138 467 23 335 246 1012
Cuts at: 3109 3195 3207 3553 1
Size: 86 12 346 449
Fragments arranged by size:
1012 779 467 449 346 335 246 138
108 86 23 12
BtgI C'CryG_G
Cuts at: 107 765 107
Size: 658 3343
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 1951 2132 2295 1951
Size: 181 163 3657
Fragments arranged by size:
3657 181 163
BtsI GCAGTG_nn'
Cuts at: 1244 1244
Size: 4001
DraI TTT'AAA
Cuts at: 1000 1000
Size: 4001
DrdI GACnn_nn'nnGTC
Cuts at: 230 1707 2565 230
Size: 1477 858 1666
Fragments arranged by size:
1666 1477 858
EaeI y'GGCC_r
Cuts at: 110 179 938 1586 1760 2151 2178 3523 110
Size: 69 759 648 174 391 27 1345 588
Fragments arranged by size:
1345 759 648 588 391 174 69 27
EagI C'GGCC_G
Cuts at: 1586 1586
Size: 4001
EarI CTCTTCn'nnn_
Cuts at: 234 868 2024 2234 234
Size: 634 1156 210 2001
Fragments arranged by size:
2001 1156 634 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 793 2209 2519 2665 3811 793
Size: 1416 310 146 1146 983
Fragments arranged by size:
1416 1146 983 310 146
EcoICRI GAG'CTC
Cuts at: 1013 1013
Size: 4001
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 569 818 902 1826 2258 3005 3273 569
Size: 249 84 924 432 747 268 1297
Fragments arranged by size:
1297 924 747 432 268 249 84
EcoO109I rG'GnC_Cy
Cuts at: 134 141 159 1140 134
Size: 7 18 981 2995
Fragments arranged by size:
2995 981 18 7
EcoRI G'AATT_C
Cuts at: 1005 1005
Size: 4001
EcoRV GAT'ATC
Cuts at: 846 846
Size: 4001
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 823 2020 823
Size: 1197 2804
FspI TGC'GCA
Cuts at: 1782 1782
Size: 4001
HaeII r_GCGC'y
Cuts at: 1435 1683 2705 3684 1435
Size: 248 1022 979 1752
Fragments arranged by size:
1752 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 797 829 1843 1873 1875 1905 797
Size: 32 1014 30 2 30 2893
Fragments arranged by size:
2893 1014 32 30 30 2
HincII GTy'rAC
Cuts at: 448 1040 448
Size: 592 3409
KasI G'GCGC_C
Cuts at: 1431 1679 1431
Size: 248 3753
KpnI G_GTAC'C
Cuts at: 742 774 1021 742
Size: 32 247 3722
Fragments arranged by size:
3722 247 32
MluI A'CGCG_T
Cuts at: 3434 3434
Size: 4001
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 214 2672 2856 214
Size: 2458 184 1359
Fragments arranged by size:
2458 1359 184
MscI TGG'CCA
Cuts at: 181 1762 181
Size: 1581 2420
MslI CAynn'nnrTG
Cuts at: 1990 2117 1990
Size: 127 3874
MspA1I CmG'CkG
Cuts at: 525 1112 1426 1786 2799 3044 525
Size: 587 314 360 1013 245 1482
Fragments arranged by size:
1482 1013 587 360 314 245
NaeI GCC'GGC
Cuts at: 2182 2182
Size: 4001
NarI GG'CG_CC
Cuts at: 1432 1680 1432
Size: 248 3753
NcoI C'CATG_G
Cuts at: 107 107
Size: 4001
NgoMIV G'CCGG_C
Cuts at: 2180 2180
Size: 4001
NspI r_CATG'y
Cuts at: 1054 1554 2461 1054
Size: 500 907 2594
Fragments arranged by size:
2594 907 500
PciI A'CATG_T
Cuts at: 2457 2457
Size: 4001
PfoI T'CCnGG_A
Cuts at: 918 918
Size: 4001
PmeI GTTT'AAAC
Cuts at: 1000 1000
Size: 4001
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3140 3172 3140
Size: 32 3969
PspOMI G'GGCC_C
Cuts at: 134 140 134
Size: 6 3995
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 754 786 754
Size: 32 3969
PstI C_TGCA'G
Cuts at: 413 1048 1733 413
Size: 635 685 2681
Fragments arranged by size:
2681 685 635
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4001
PvuII CAG'CTG
Cuts at: 525 1426 1786 525
Size: 901 360 2740
Fragments arranged by size:
2740 901 360
RsrII CG'GwC_CG
Cuts at: 2196 2196
Size: 4001
SacI G_AGCT'C
Cuts at: 1015 1015
Size: 4001
SalI G'TCGA_C
Cuts at: 1038 1038
Size: 4001
SapI GCTCTTCn'nnn_
Cuts at: 2024 2234 2024
Size: 210 3791
SbfI CC_TGCA'GG
Cuts at: 1048 1048
Size: 4001
ScaI AGT'ACT
Cuts at: 3518 3518
Size: 4001
SfcI C'TryA_G
Cuts at: 33 409 632 962 1044 1729 2722 2913 33
Size: 376 223 330 82 685 993 191 1121
Fragments arranged by size:
1121 993 685 376 330 223 191 82
SfoI GGC'GCC
Cuts at: 1433 1681 1433
Size: 248 3753
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4001
SmaI CCC'GGG
Cuts at: 139 1023 139
Size: 884 3117
SmlI C'TyrA_G
Cuts at: 979 1156 2071 2563 2825 979
Size: 177 915 492 262 2155
Fragments arranged by size:
2155 915 492 262 177
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4001
SphI G_CATG'C
Cuts at: 1054 1554 1054
Size: 500 3501
SrfI GCCC'GGGC
Cuts at: 139 139
Size: 4001
SspI AAT'ATT
Cuts at: 567 567
Size: 4001
StyI C'CwwG_G
Cuts at: 107 892 1135 1489 107
Size: 785 243 354 2619
Fragments arranged by size:
2619 785 354 243
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 915 1584 1717 2176 2385 915
Size: 669 133 459 209 2531
Fragments arranged by size:
2531 669 459 209 133
TatI w'GTAC_w
Cuts at: 573 877 3516 573
Size: 304 2639 1058
Fragments arranged by size:
2639 1058 304
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 914 946 3518 3550 914
Size: 32 2572 32 1365
Fragments arranged by size:
2572 1365 32 32
Tth111I GACn'n_nGTC
Cuts at: 1798 1798
Size: 4001
XbaI T'CTAG_A
Cuts at: 1032 1032
Size: 4001
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 472 1496 472
Size: 1024 2977
XmaI C'CCGG_G
Cuts at: 137 1021 137
Size: 884 3117
Enzymes that do cut:
AccI Acc65I AclI AcuI AflII AflIII AgeI AlwNI
ApaI ApaLI ApoI AseI AsiSI AvaI BaeI BamHI
BanI BanII BbeI BbsI BciVI BclI BglII BlpI
Bme1580I BmrI BplI BpmI BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseRI BseYI BsiEI BsiHKAI BsmI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BssSI
BstAPI BstBI BstYI BtgI BtgZI BtsI DraI DrdI
EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI
EcoRV FalI FspI HaeII Hin4I HincII KasI KpnI
MluI MmeI MscI MslI MspA1I NaeI NarI NcoI
NgoMIV NspI PciI PfoI PmeI PpiI PspOMI PsrI
PstI PvuI PvuII RsrII SacI SalI SapI SbfI
ScaI SfcI SfoI SgfI SmaI SmlI SpeI SphI
SrfI SspI StyI TaqII TatI TstI Tth111I XbaI
XcmI XmaI
Enzymes that do not cut:
AarI AatII AfeI AhdI AleI AlfI AloI AscI
AvrII BbvCI BcgI BfrBI BglI BmgBI BmtI Bpu10I
BsgI BsiWI BsmBI BsrGI BssHII BstEII BstXI BstZ17I
Bsu36I ClaI CspCI DraIII EcoNI FseI FspAI HindIII
HpaI MfeI NdeI NheI NotI NruI NsiI PacI
PasI PflMI PmlI PpuMI PshAI PsiI PspXI SacII
SanDI SexAI SfiI SgrAI SnaBI StuI SwaI XhoI
XmnI ZraI