Restriction map for
pF1KE0142
(Circular) (Six-base) MAPSORT of: pF1KE0142.seq Check: 7038 from: 1 to: 3650
pF1KE0142 3650 bp
With 182 enzymes: SgfI *
April 24, 2009 13:11 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 513 513
Size: 3650
AccI GT'mk_AC
Cuts at: 688 688
Size: 3650
Acc65I G'GTAC_C
Cuts at: 666 666
Size: 3650
AclI AA'CG_TT
Cuts at: 3490 3490
Size: 3650
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1475 1907 2654 2922 1475
Size: 432 747 268 2203
Fragments arranged by size:
2203 747 432 268
AfeI AGC'GCT
Cuts at: 512 512
Size: 3650
AflIII A'CryG_T
Cuts at: 1002 2106 3083 1002
Size: 1104 977 1569
Fragments arranged by size:
1569 1104 977
AgeI A'CCGG_T
Cuts at: 2038 2038
Size: 3650
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 473 589 473
Size: 116 3534
AlwNI CAG_nnn'CTG
Cuts at: 631 955 2522 631
Size: 324 1567 1759
Fragments arranged by size:
1759 1567 324
ApaLI G'TGCA_C
Cuts at: 2420 2420
Size: 3650
ApoI r'AATT_y
Cuts at: 654 3003 3259 654
Size: 2349 256 1045
Fragments arranged by size:
2349 1045 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 3650
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 3650
AvaI C'yCGr_G
Cuts at: 670 670
Size: 3650
BamHI G'GATC_C
Cuts at: 675 675
Size: 3650
BanI G'GyrC_C
Cuts at: 413 534 547 666 1080 1328 1363 413
Size: 121 13 119 414 248 35 2700
Fragments arranged by size:
2700 414 248 121 119 35 13
BanII G_rGCy'C
Cuts at: 664 1694 664
Size: 1030 2620
BbeI G_GCGC'C
Cuts at: 417 538 551 1084 1332 417
Size: 121 13 533 248 2735
Fragments arranged by size:
2735 533 248 121 13
BciVI GTATCCnnnnn_n'
Cuts at: 1548 2309 3650 1548
Size: 761 1341 1548
Fragments arranged by size:
1548 1341 761
BclI T'GATC_A
Cuts at: 1170 1170
Size: 3650
BglII A'GATC_T
Cuts at: 1165 3202 1165
Size: 2037 1613
BlpI GC'TnA_GC
Cuts at: 762 762
Size: 3650
Bme1580I G_kGCm'C
Cuts at: 304 1275 1368 2424 304
Size: 971 93 1056 1530
Fragments arranged by size:
1530 1056 971 93
BmrI ACTGGGnnnn_n'
Cuts at: 267 862 943 1278 3422 267
Size: 595 81 335 2144 495
Fragments arranged by size:
2144 595 495 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2017 2017
Size: 3650
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 424 574 424
Size: 150 3500
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 826 1705 2197 2495 826
Size: 879 492 298 1981
Fragments arranged by size:
1981 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 3354 3354
Size: 3650
BsaAI yAC'GTr
Cuts at: 1003 1003
Size: 3650
BsaBI GATnn'nnATC
Cuts at: 6 283 1164 3201 6
Size: 277 881 2037 455
Fragments arranged by size:
2037 881 455 277
BsaHI Gr'CG_yC
Cuts at: 414 535 548 1081 1329 2031 3527 414
Size: 121 13 533 248 702 1496 537
Fragments arranged by size:
1496 702 537 533 248 121 13
BsaWI w'CCGG_w
Cuts at: 839 1360 2038 2312 2459 2870 839
Size: 521 678 274 147 411 1619
Fragments arranged by size:
1619 678 521 411 274 147
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 340 457 460 340
Size: 117 3 3530
Fragments arranged by size:
3530 117 3
BseYI C'CCAG_C
Cuts at: 342 357 590 1075 1833 2410 2906 342
Size: 15 233 485 758 577 496 1086
Fragments arranged by size:
1086 758 577 496 485 233 15
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 547 547
Size: 3650
BsiEI CG_ry'CG
Cuts at: 104 609 1192 1238 2446 104
Size: 505 583 46 1208 1308
Fragments arranged by size:
1308 1208 583 505 46
BsiHKAI G_wGCw'C
Cuts at: 664 1442 1632 2424 664
Size: 778 190 792 1890
Fragments arranged by size:
1890 792 778 190
Bsp1286I G_dGCh'C
Cuts at: 304 664 1275 1368 1442 1632 1694 2424 304
Size: 360 611 93 74 190 62 730 1530
Fragments arranged by size:
1530 730 611 360 190 93 74 62
BspEI T'CCGG_A
Cuts at: 2870 2870
Size: 3650
BspHI T'CATG_A
Cuts at: 2826 2826
Size: 3650
BspMI ACCTGCnnnn'nnnn_
Cuts at: 150 513 700 914 1216 1597 150
Size: 363 187 214 302 381 2203
Fragments arranged by size:
2203 381 363 302 214 187
BsrBI CCG'CTC
Cuts at: 50 1942 1996 3480 3645 50
Size: 1892 54 1484 165 55
Fragments arranged by size:
1892 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 1562 1562
Size: 3650
BsrFI r'CCGG_y
Cuts at: 240 608 1648 1829 2038 240
Size: 368 1040 181 209 1852
Fragments arranged by size:
1852 1040 368 209 181
BssHII G'CGCG_C
Cuts at: 585 585
Size: 3650
BssSI C'ACGA_G
Cuts at: 1921 2279 1921
Size: 358 3292
BstAPI GCAn_nnn'nTGC
Cuts at: 582 631 1027 582
Size: 49 396 3205
Fragments arranged by size:
3205 396 49
BstBI TT'CG_AA
Cuts at: 2011 2011
Size: 3650
BstXI CCAn_nnnn'nTGG
Cuts at: 114 114
Size: 3650
BstYI r'GATC_y
Cuts at: 1 675 1142 1165 1500 1746 2758 2844 2856
Size: 674 467 23 335 246 1012 86 12
Cuts at: 2856 3202 1
Size: 346 449
Fragments arranged by size:
1012 674 467 449 346 335 246 86
23 12
BtgI C'CryG_G
Cuts at: 107 107
Size: 3650
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 1600 1781 1944 1600
Size: 181 163 3306
Fragments arranged by size:
3306 181 163
BtsI GCAGTG_nn'
Cuts at: 632 893 632
Size: 261 3389
DraI TTT'AAA
Cuts at: 649 649
Size: 3650
DraIII CAC_nnn'GTG
Cuts at: 261 627 261
Size: 366 3284
DrdI GACnn_nn'nnGTC
Cuts at: 1356 2214 1356
Size: 858 2792
EaeI y'GGCC_r
Cuts at: 606 1235 1409 1800 1827 3172 606
Size: 629 174 391 27 1345 1084
Fragments arranged by size:
1345 1084 629 391 174 27
EagI C'GGCC_G
Cuts at: 606 1235 606
Size: 629 3021
EarI CTCTTCn'nnn_
Cuts at: 1673 1883 1673
Size: 210 3440
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 1858 2168 2314 3460 1858
Size: 310 146 1146 2048
Fragments arranged by size:
2048 1146 310 146
EcoICRI GAG'CTC
Cuts at: 662 662
Size: 3650
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 424 574 1475 1907 2654 2922 424
Size: 150 901 432 747 268 1152
Fragments arranged by size:
1152 901 747 432 268 150
EcoO109I rG'GnC_Cy
Cuts at: 789 789
Size: 3650
EcoRI G'AATT_C
Cuts at: 654 654
Size: 3650
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 1669 1669
Size: 3650
FspI TGC'GCA
Cuts at: 283 565 1431 283
Size: 282 866 2502
Fragments arranged by size:
2502 866 282
FspAI rTGC'GCAy
Cuts at: 283 283
Size: 3650
HaeII r_GCGC'y
Cuts at: 417 435 499 505 514 538 551 1084 1332
Size: 18 64 6 9 24 13 533 248
Cuts at: 1332 2354 3333 417
Size: 1022 979 734
Fragments arranged by size:
1022 979 734 533 248 64 24 18
13 9 6
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1492 1522 1524 1554 1492
Size: 30 2 30 3588
Fragments arranged by size:
3588 30 30 2
HincII GTy'rAC
Cuts at: 689 689
Size: 3650
KasI G'GCGC_C
Cuts at: 413 534 547 1080 1328 413
Size: 121 13 533 248 2735
Fragments arranged by size:
2735 533 248 121 13
KpnI G_GTAC'C
Cuts at: 670 670
Size: 3650
MluI A'CGCG_T
Cuts at: 3083 3083
Size: 3650
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 2321 2505 2321
Size: 184 3466
MscI TGG'CCA
Cuts at: 1411 1411
Size: 3650
MslI CAynn'nnrTG
Cuts at: 112 1639 1766 112
Size: 1527 127 1996
Fragments arranged by size:
1996 1527 127
MspA1I CmG'CkG
Cuts at: 318 761 1075 1435 2448 2693 318
Size: 443 314 360 1013 245 1275
Fragments arranged by size:
1275 1013 443 360 314 245
NaeI GCC'GGC
Cuts at: 242 1831 242
Size: 1589 2061
NarI GG'CG_CC
Cuts at: 414 535 548 1081 1329 414
Size: 121 13 533 248 2735
Fragments arranged by size:
2735 533 248 121 13
NcoI C'CATG_G
Cuts at: 107 107
Size: 3650
NgoMIV G'CCGG_C
Cuts at: 240 1829 240
Size: 1589 2061
NspI r_CATG'y
Cuts at: 703 1203 2110 703
Size: 500 907 2243
Fragments arranged by size:
2243 907 500
PciI A'CATG_T
Cuts at: 2106 2106
Size: 3650
PmeI GTTT'AAAC
Cuts at: 649 649
Size: 3650
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2789 2821 2789
Size: 32 3618
PstI C_TGCA'G
Cuts at: 518 697 1382 518
Size: 179 685 2786
Fragments arranged by size:
2786 685 179
PvuI CG_AT'CG
Cuts at: 104 104
Size: 3650
PvuII CAG'CTG
Cuts at: 1075 1435 1075
Size: 360 3290
RsrII CG'GwC_CG
Cuts at: 611 1845 611
Size: 1234 2416
SacI G_AGCT'C
Cuts at: 664 664
Size: 3650
SalI G'TCGA_C
Cuts at: 687 687
Size: 3650
SapI GCTCTTCn'nnn_
Cuts at: 1673 1883 1673
Size: 210 3440
SbfI CC_TGCA'GG
Cuts at: 697 697
Size: 3650
ScaI AGT'ACT
Cuts at: 409 3167 409
Size: 2758 892
SfcI C'TryA_G
Cuts at: 33 514 693 1378 2371 2562 33
Size: 481 179 685 993 191 1121
Fragments arranged by size:
1121 993 685 481 191 179
SfoI GGC'GCC
Cuts at: 415 536 549 1082 1330 415
Size: 121 13 533 248 2735
Fragments arranged by size:
2735 533 248 121 13
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 3650
SmaI CCC'GGG
Cuts at: 672 672
Size: 3650
SmlI C'TyrA_G
Cuts at: 805 1720 2212 2474 805
Size: 915 492 262 1981
Fragments arranged by size:
1981 915 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 3650
SphI G_CATG'C
Cuts at: 703 1203 703
Size: 500 3150
StyI C'CwwG_G
Cuts at: 107 784 1138 107
Size: 677 354 2619
Fragments arranged by size:
2619 677 354
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 249 1233 1366 1825 2034 249
Size: 984 133 459 209 1865
Fragments arranged by size:
1865 984 459 209 133
TatI w'GTAC_w
Cuts at: 407 3165 407
Size: 2758 892
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3167 3199 3167
Size: 32 3618
Tth111I GACn'n_nGTC
Cuts at: 1447 1447
Size: 3650
XbaI T'CTAG_A
Cuts at: 681 681
Size: 3650
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 378 1145 378
Size: 767 2883
XmaI C'CCGG_G
Cuts at: 670 670
Size: 3650
Enzymes that do cut:
AarI AccI Acc65I AclI AcuI AfeI AflIII AgeI
AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI
BanI BanII BbeI BciVI BclI BglII BlpI Bme1580I
BmrI BplI BpmI BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BseRI BseYI BsgI BsiEI BsiHKAI Bsp1286I BspEI
BspHI BspMI BsrBI BsrDI BsrFI BssHII BssSI BstAPI
BstBI BstXI BstYI BtgI BtgZI BtsI DraI DraIII
DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I
EcoRI FalI FspI FspAI HaeII Hin4I HincII KasI
KpnI MluI MmeI MscI MslI MspA1I NaeI NarI
NcoI NgoMIV NspI PciI PmeI PpiI PstI PvuI
PvuII RsrII SacI SalI SapI SbfI ScaI SfcI
SfoI SgfI SmaI SmlI SpeI SphI StyI TaqII
TatI TstI Tth111I XbaI XcmI XmaI
Enzymes that do not cut:
AatII AflII AhdI AleI AloI ApaI AscI AvrII
BaeI BbsI BbvCI BcgI BfrBI BglI BmgBI BmtI
Bpu10I BsaXI BsiWI BsmI BsmBI BsrGI BstEII BstZ17I
Bsu36I ClaI CspCI EcoNI EcoRV FseI HindIII HpaI
MfeI NdeI NheI NotI NruI NsiI PacI PasI
PflMI PfoI PmlI PpuMI PshAI PsiI PspOMI PspXI
PsrI SacII SanDI SexAI SfiI SgrAI SnaBI SrfI
SspI StuI SwaI XhoI XmnI ZraI