Restriction map for
pF1KE0141
(Circular) (Six-base) MAPSORT of: pF1KE0141.seq Check: 560 from: 1 to: 3785
pF1KE0141 3785 bp
With 182 enzymes: SgfI *
April 24, 2009 13:11 ..
AccI GT'mk_AC
Cuts at: 823 823
Size: 3785
Acc65I G'GTAC_C
Cuts at: 801 801
Size: 3785
AclI AA'CG_TT
Cuts at: 3625 3625
Size: 3785
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 265 565 1610 2042 2789 3057 265
Size: 300 1045 432 747 268 993
Fragments arranged by size:
1045 993 747 432 300 268
AfeI AGC'GCT
Cuts at: 349 349
Size: 3785
AflIII A'CryG_T
Cuts at: 1137 2241 3218 1137
Size: 1104 977 1704
Fragments arranged by size:
1704 1104 977
AgeI A'CCGG_T
Cuts at: 699 2173 699
Size: 1474 2311
AlwNI CAG_nnn'CTG
Cuts at: 1090 2657 1090
Size: 1567 2218
ApaLI G'TGCA_C
Cuts at: 2555 2555
Size: 3785
ApoI r'AATT_y
Cuts at: 789 3138 3394 789
Size: 2349 256 1180
Fragments arranged by size:
2349 1180 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 3785
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 3785
AvaI C'yCGr_G
Cuts at: 268 364 422 805 268
Size: 96 58 383 3248
Fragments arranged by size:
3248 383 96 58
BamHI G'GATC_C
Cuts at: 810 810
Size: 3785
BanI G'GyrC_C
Cuts at: 801 1215 1463 1498 801
Size: 414 248 35 3088
Fragments arranged by size:
3088 414 248 35
BanII G_rGCy'C
Cuts at: 233 277 799 1829 233
Size: 44 522 1030 2189
Fragments arranged by size:
2189 1030 522 44
BbeI G_GCGC'C
Cuts at: 1219 1467 1219
Size: 248 3537
BciVI GTATCCnnnnn_n'
Cuts at: 1683 2444 3785 1683
Size: 761 1341 1683
Fragments arranged by size:
1683 1341 761
BclI T'GATC_A
Cuts at: 1305 1305
Size: 3785
BglII A'GATC_T
Cuts at: 1300 3337 1300
Size: 2037 1748
BlpI GC'TnA_GC
Cuts at: 897 897
Size: 3785
Bme1580I G_kGCm'C
Cuts at: 515 1410 1503 2559 515
Size: 895 93 1056 1741
Fragments arranged by size:
1741 1056 895 93
BmgBI CAC'GTC
Cuts at: 198 429 198
Size: 231 3554
BmrI ACTGGGnnnn_n'
Cuts at: 686 997 1078 1413 3557 686
Size: 311 81 335 2144 914
Fragments arranged by size:
2144 914 335 311 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 612 2152 612
Size: 1540 2245
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 343 343
Size: 3785
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 541 961 1840 2332 2630 541
Size: 420 879 492 298 1696
Fragments arranged by size:
1696 879 492 420 298
BsaI GGTCTCn'nnnn_
Cuts at: 3489 3489
Size: 3785
BsaAI yAC'GTr
Cuts at: 387 1138 387
Size: 751 3034
BsaBI GATnn'nnATC
Cuts at: 6 1299 3336 6
Size: 1293 2037 455
Fragments arranged by size:
2037 1293 455
BsaHI Gr'CG_yC
Cuts at: 487 682 1216 1464 2166 3662 487
Size: 195 534 248 702 1496 610
Fragments arranged by size:
1496 702 610 534 248 195
BsaWI w'CCGG_w
Cuts at: 699 974 1495 2173 2447 2594 3005 699
Size: 275 521 678 274 147 411 1479
Fragments arranged by size:
1479 678 521 411 275 274 147
BseYI C'CCAG_C
Cuts at: 327 1210 1968 2545 3041 327
Size: 883 758 577 496 1071
Fragments arranged by size:
1071 883 758 577 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 295 451 295
Size: 156 3629
BsiEI CG_ry'CG
Cuts at: 104 1327 1373 2581 104
Size: 1223 46 1208 1308
Fragments arranged by size:
1308 1223 1208 46
BsiHKAI G_wGCw'C
Cuts at: 799 1577 1767 2559 799
Size: 778 190 792 2025
Fragments arranged by size:
2025 792 778 190
Bsp1286I G_dGCh'C
Cuts at: 233 277 515 799 1410 1503 1577 1767 1829
Size: 44 238 284 611 93 74 190 62
Cuts at: 1829 2559 233
Size: 730 1459
Fragments arranged by size:
1459 730 611 284 238 190 93 74
62 44
BspEI T'CCGG_A
Cuts at: 3005 3005
Size: 3785
BspHI T'CATG_A
Cuts at: 2961 2961
Size: 3785
BspMI ACCTGCnnnn'nnnn_
Cuts at: 317 835 1049 1351 1732 317
Size: 518 214 302 381 2370
Fragments arranged by size:
2370 518 381 302 214
BsrBI CCG'CTC
Cuts at: 50 2077 2131 3615 3780 50
Size: 2027 54 1484 165 55
Fragments arranged by size:
2027 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 1697 1697
Size: 3785
BsrFI r'CCGG_y
Cuts at: 699 1783 1964 2173 699
Size: 1084 181 209 2311
Fragments arranged by size:
2311 1084 209 181
BssSI C'ACGA_G
Cuts at: 316 2056 2414 316
Size: 1740 358 1687
Fragments arranged by size:
1740 1687 358
BstAPI GCAn_nnn'nTGC
Cuts at: 350 1162 350
Size: 812 2973
BstBI TT'CG_AA
Cuts at: 2146 2146
Size: 3785
BstYI r'GATC_y
Cuts at: 1 524 810 1277 1300 1635 1881 2893 2979
Size: 523 286 467 23 335 246 1012 86
Cuts at: 2979 2991 3337 1
Size: 12 346 449
Fragments arranged by size:
1012 523 467 449 346 335 286 246
86 23 12
BtgI C'CryG_G
Cuts at: 107 254 377 107
Size: 147 123 3515
Fragments arranged by size:
3515 147 123
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 1735 1916 2079 1735
Size: 181 163 3441
Fragments arranged by size:
3441 181 163
BtsI GCAGTG_nn'
Cuts at: 1028 1028
Size: 3785
DraI TTT'AAA
Cuts at: 759 784 759
Size: 25 3760
DrdI GACnn_nn'nnGTC
Cuts at: 1491 2349 1491
Size: 858 2927
EaeI y'GGCC_r
Cuts at: 374 1370 1544 1935 1962 3307 374
Size: 996 174 391 27 1345 852
Fragments arranged by size:
1345 996 852 391 174 27
EagI C'GGCC_G
Cuts at: 1370 1370
Size: 3785
EarI CTCTTCn'nnn_
Cuts at: 469 1808 2018 469
Size: 1339 210 2236
Fragments arranged by size:
2236 1339 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 1993 2303 2449 3595 1993
Size: 310 146 1146 2183
Fragments arranged by size:
2183 1146 310 146
EcoICRI GAG'CTC
Cuts at: 797 797
Size: 3785
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 265 343 565 1610 2042 2789 3057 265
Size: 78 222 1045 432 747 268 993
Fragments arranged by size:
1045 993 747 432 268 222 78
EcoO109I rG'GnC_Cy
Cuts at: 924 924
Size: 3785
EcoRI G'AATT_C
Cuts at: 789 789
Size: 3785
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 1804 1804
Size: 3785
FspI TGC'GCA
Cuts at: 211 1566 211
Size: 1355 2430
HaeII r_GCGC'y
Cuts at: 351 1219 1467 2489 3468 351
Size: 868 248 1022 979 668
Fragments arranged by size:
1022 979 868 668 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 653 685 1627 1657 1659 1689 653
Size: 32 942 30 2 30 2749
Fragments arranged by size:
2749 942 32 30 30 2
HincII GTy'rAC
Cuts at: 564 824 564
Size: 260 3525
KasI G'GCGC_C
Cuts at: 1215 1463 1215
Size: 248 3537
KpnI G_GTAC'C
Cuts at: 805 805
Size: 3785
MluI A'CGCG_T
Cuts at: 3218 3218
Size: 3785
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 675 2456 2640 675
Size: 1781 184 1820
Fragments arranged by size:
1820 1781 184
MscI TGG'CCA
Cuts at: 1546 1546
Size: 3785
MslI CAynn'nnrTG
Cuts at: 286 1774 1901 286
Size: 1488 127 2170
Fragments arranged by size:
2170 1488 127
MspA1I CmG'CkG
Cuts at: 896 1210 1570 2583 2828 896
Size: 314 360 1013 245 1853
Fragments arranged by size:
1853 1013 360 314 245
NaeI GCC'GGC
Cuts at: 1966 1966
Size: 3785
NarI GG'CG_CC
Cuts at: 1216 1464 1216
Size: 248 3537
NcoI C'CATG_G
Cuts at: 107 377 107
Size: 270 3515
NgoMIV G'CCGG_C
Cuts at: 1964 1964
Size: 3785
NspI r_CATG'y
Cuts at: 838 1338 2245 838
Size: 500 907 2378
Fragments arranged by size:
2378 907 500
PciI A'CATG_T
Cuts at: 2241 2241
Size: 3785
PflMI CCAn_nnn'nTGG
Cuts at: 321 321
Size: 3785
PmeI GTTT'AAAC
Cuts at: 784 784
Size: 3785
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2924 2956 2924
Size: 32 3753
PstI C_TGCA'G
Cuts at: 832 1517 832
Size: 685 3100
PvuI CG_AT'CG
Cuts at: 104 104
Size: 3785
PvuII CAG'CTG
Cuts at: 1210 1570 1210
Size: 360 3425
RsrII CG'GwC_CG
Cuts at: 1980 1980
Size: 3785
SacI G_AGCT'C
Cuts at: 799 799
Size: 3785
SalI G'TCGA_C
Cuts at: 822 822
Size: 3785
SapI GCTCTTCn'nnn_
Cuts at: 469 1808 2018 469
Size: 1339 210 2236
Fragments arranged by size:
2236 1339 210
SbfI CC_TGCA'GG
Cuts at: 832 832
Size: 3785
ScaI AGT'ACT
Cuts at: 3302 3302
Size: 3785
SfcI C'TryA_G
Cuts at: 33 828 1513 2506 2697 33
Size: 795 685 993 191 1121
Fragments arranged by size:
1121 993 795 685 191
SfoI GGC'GCC
Cuts at: 1217 1465 1217
Size: 248 3537
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 3785
SmaI CCC'GGG
Cuts at: 424 807 424
Size: 383 3402
SmlI C'TyrA_G
Cuts at: 556 940 1855 2347 2609 556
Size: 384 915 492 262 1732
Fragments arranged by size:
1732 915 492 384 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 3785
SphI G_CATG'C
Cuts at: 838 1338 838
Size: 500 3285
StyI C'CwwG_G
Cuts at: 107 377 515 650 919 1273 107
Size: 270 138 135 269 354 2619
Fragments arranged by size:
2619 354 270 269 138 135
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1368 1501 1960 2169 1368
Size: 133 459 209 2984
Fragments arranged by size:
2984 459 209 133
TatI w'GTAC_w
Cuts at: 3300 3300
Size: 3785
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3302 3334 3302
Size: 32 3753
Tth111I GACn'n_nGTC
Cuts at: 196 1582 196
Size: 1386 2399
XbaI T'CTAG_A
Cuts at: 816 816
Size: 3785
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 114 384 1280 114
Size: 270 896 2619
Fragments arranged by size:
2619 896 270
XmaI C'CCGG_G
Cuts at: 422 805 422
Size: 383 3402
XmnI GAAnn'nnTTC
Cuts at: 478 478
Size: 3785
Enzymes that do cut:
AccI Acc65I AclI AcuI AfeI AflIII AgeI AlwNI
ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII
BbeI BciVI BclI BglII BlpI Bme1580I BmgBI BmrI
BplI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BseYI BsgI BsiEI BsiHKAI Bsp1286I BspEI BspHI BspMI
BsrBI BsrDI BsrFI BssSI BstAPI BstBI BstYI BtgI
BtgZI BtsI DraI DrdI EaeI EagI EarI EciI
EcoICRI Eco57MI EcoO109I EcoRI FalI FspI HaeII Hin4I
HincII KasI KpnI MluI MmeI MscI MslI MspA1I
NaeI NarI NcoI NgoMIV NspI PciI PflMI PmeI
PpiI PstI PvuI PvuII RsrII SacI SalI SapI
SbfI ScaI SfcI SfoI SgfI SmaI SmlI SpeI
SphI StyI TaqII TatI TstI Tth111I XbaI XcmI
XmaI XmnI
Enzymes that do not cut:
AarI AatII AflII AhdI AleI AlfI AloI ApaI
AscI AvrII BaeI BbsI BbvCI BcgI BfrBI BglI
BmtI Bpu10I BsaXI BseRI BsiWI BsmI BsmBI BsrGI
BssHII BstEII BstXI BstZ17I Bsu36I ClaI CspCI DraIII
EcoNI EcoRV FseI FspAI HindIII HpaI MfeI NdeI
NheI NotI NruI NsiI PacI PasI PfoI PmlI
PpuMI PshAI PsiI PspOMI PspXI PsrI SacII SanDI
SexAI SfiI SgrAI SnaBI SrfI SspI StuI SwaI
XhoI ZraI