Restriction map for
pF1KE0140
(Circular) (Six-base) MAPSORT of: pF1KE0140.seq Check: 3936 from: 1 to: 3968
pF1KE0140 3968 bp
With 182 enzymes: SgfI *
April 24, 2009 13:11 ..
AccI GT'mk_AC
Cuts at: 581 1006 581
Size: 425 3543
Acc65I G'GTAC_C
Cuts at: 984 984
Size: 3968
AclI AA'CG_TT
Cuts at: 3808 3808
Size: 3968
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 920 1793 2225 2972 3240 920
Size: 873 432 747 268 1648
Fragments arranged by size:
1648 873 747 432 268
AflII C'TTAA_G
Cuts at: 600 600
Size: 3968
AflIII A'CryG_T
Cuts at: 1320 2424 3401 1320
Size: 1104 977 1887
Fragments arranged by size:
1887 1104 977
AgeI A'CCGG_T
Cuts at: 452 2356 452
Size: 1904 2064
AlwNI CAG_nnn'CTG
Cuts at: 625 1273 2840 625
Size: 648 1567 1753
Fragments arranged by size:
1753 1567 648
ApaLI G'TGCA_C
Cuts at: 2738 2738
Size: 3968
ApoI r'AATT_y
Cuts at: 314 440 972 3321 3577 314
Size: 126 532 2349 256 705
Fragments arranged by size:
2349 705 532 256 126
AseI AT'TA_AT
Cuts at: 20 20
Size: 3968
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 3968
AvaI C'yCGr_G
Cuts at: 167 988 167
Size: 821 3147
BamHI G'GATC_C
Cuts at: 993 993
Size: 3968
BanI G'GyrC_C
Cuts at: 114 126 984 1398 1646 1681 114
Size: 12 858 414 248 35 2401
Fragments arranged by size:
2401 858 414 248 35 12
BanII G_rGCy'C
Cuts at: 251 982 2012 251
Size: 731 1030 2207
Fragments arranged by size:
2207 1030 731
BbeI G_GCGC'C
Cuts at: 1402 1650 1402
Size: 248 3720
BciVI GTATCCnnnnn_n'
Cuts at: 1866 2627 3968 1866
Size: 761 1341 1866
Fragments arranged by size:
1866 1341 761
BclI T'GATC_A
Cuts at: 1488 1488
Size: 3968
BglI GCCn_nnn'nGGC
Cuts at: 665 665
Size: 3968
BglII A'GATC_T
Cuts at: 1483 3520 1483
Size: 2037 1931
BlpI GC'TnA_GC
Cuts at: 1080 1080
Size: 3968
Bme1580I G_kGCm'C
Cuts at: 706 1593 1686 2742 706
Size: 887 93 1056 1932
Fragments arranged by size:
1932 1056 887 93
BmrI ACTGGGnnnn_n'
Cuts at: 1180 1261 1596 3740 1180
Size: 81 335 2144 1408
Fragments arranged by size:
2144 1408 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2335 2335
Size: 3968
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 232 1144 2023 2515 2813 232
Size: 912 879 492 298 1387
Fragments arranged by size:
1387 912 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 3672 3672
Size: 3968
BsaAI yAC'GTr
Cuts at: 1321 1321
Size: 3968
BsaBI GATnn'nnATC
Cuts at: 6 1482 3519 6
Size: 1476 2037 455
Fragments arranged by size:
2037 1476 455
BsaHI Gr'CG_yC
Cuts at: 1399 1647 2349 3845 1399
Size: 248 702 1496 1522
Fragments arranged by size:
1522 1496 702 248
BsaWI w'CCGG_w
Cuts at: 452 1157 1678 2356 2630 2777 3188 452
Size: 705 521 678 274 147 411 1232
Fragments arranged by size:
1232 705 678 521 411 274 147
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 229 229
Size: 3968
BseYI C'CCAG_C
Cuts at: 371 518 699 1393 2151 2728 3224 371
Size: 147 181 694 758 577 496 1115
Fragments arranged by size:
1115 758 694 577 496 181 147
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 458 458
Size: 3968
BsiEI CG_ry'CG
Cuts at: 104 1510 1556 2764 104
Size: 1406 46 1208 1308
Fragments arranged by size:
1406 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 842 982 1760 1950 2742 842
Size: 140 778 190 792 2068
Fragments arranged by size:
2068 792 778 190 140
Bsp1286I G_dGCh'C
Cuts at: 251 706 842 982 1593 1686 1760 1950 2012
Size: 455 136 140 611 93 74 190 62
Cuts at: 2012 2742 251
Size: 730 1477
Fragments arranged by size:
1477 730 611 455 190 140 136 93
74 62
BspEI T'CCGG_A
Cuts at: 3188 3188
Size: 3968
BspHI T'CATG_A
Cuts at: 3144 3144
Size: 3968
BspMI ACCTGCnnnn'nnnn_
Cuts at: 1018 1232 1534 1915 1018
Size: 214 302 381 3071
Fragments arranged by size:
3071 381 302 214
BsrBI CCG'CTC
Cuts at: 50 2260 2314 3798 3963 50
Size: 2210 54 1484 165 55
Fragments arranged by size:
2210 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 1880 1880
Size: 3968
BsrFI r'CCGG_y
Cuts at: 123 452 1966 2147 2356 123
Size: 329 1514 181 209 1735
Fragments arranged by size:
1735 1514 329 209 181
BssSI C'ACGA_G
Cuts at: 491 2239 2597 491
Size: 1748 358 1862
Fragments arranged by size:
1862 1748 358
BstAPI GCAn_nnn'nTGC
Cuts at: 481 1345 481
Size: 864 3104
BstBI TT'CG_AA
Cuts at: 2329 2329
Size: 3968
BstYI r'GATC_y
Cuts at: 1 993 1460 1483 1818 2064 3076 3162 3174
Size: 992 467 23 335 246 1012 86 12
Cuts at: 3174 3520 1
Size: 346 449
Fragments arranged by size:
1012 992 467 449 346 335 246 86
23 12
BstZ17I GTA'TAC
Cuts at: 582 582
Size: 3968
BtgI C'CryG_G
Cuts at: 107 130 107
Size: 23 3945
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 1918 2099 2262 1918
Size: 181 163 3624
Fragments arranged by size:
3624 181 163
BtsI GCAGTG_nn'
Cuts at: 542 910 1211 542
Size: 368 301 3299
Fragments arranged by size:
3299 368 301
DraI TTT'AAA
Cuts at: 967 967
Size: 3968
DraIII CAC_nnn'GTG
Cuts at: 481 481
Size: 3968
DrdI GACnn_nn'nnGTC
Cuts at: 1674 2532 1674
Size: 858 3110
EaeI y'GGCC_r
Cuts at: 1553 1727 2118 2145 3490 1553
Size: 174 391 27 1345 2031
Fragments arranged by size:
2031 1345 391 174 27
EagI C'GGCC_G
Cuts at: 1553 1553
Size: 3968
EarI CTCTTCn'nnn_
Cuts at: 803 1991 2201 803
Size: 1188 210 2570
Fragments arranged by size:
2570 1188 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2176 2486 2632 3778 2176
Size: 310 146 1146 2366
Fragments arranged by size:
2366 1146 310 146
EcoICRI GAG'CTC
Cuts at: 980 980
Size: 3968
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 920 1793 2225 2972 3240 920
Size: 873 432 747 268 1648
Fragments arranged by size:
1648 873 747 432 268
EcoNI CCTnn'n_nnAGG
Cuts at: 713 713
Size: 3968
EcoO109I rG'GnC_Cy
Cuts at: 324 1107 324
Size: 783 3185
EcoRI G'AATT_C
Cuts at: 972 972
Size: 3968
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 1987 1987
Size: 3968
FspI TGC'GCA
Cuts at: 1749 1749
Size: 3968
HaeII r_GCGC'y
Cuts at: 519 1402 1650 2672 3651 519
Size: 883 248 1022 979 836
Fragments arranged by size:
1022 979 883 836 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1810 1840 1842 1872 1810
Size: 30 2 30 3906
Fragments arranged by size:
3906 30 30 2
HincII GTy'rAC
Cuts at: 1007 1007
Size: 3968
KasI G'GCGC_C
Cuts at: 1398 1646 1398
Size: 248 3720
KpnI G_GTAC'C
Cuts at: 988 988
Size: 3968
MluI A'CGCG_T
Cuts at: 3401 3401
Size: 3968
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 505 2639 2823 505
Size: 2134 184 1650
Fragments arranged by size:
2134 1650 184
MscI TGG'CCA
Cuts at: 1729 1729
Size: 3968
MslI CAynn'nnrTG
Cuts at: 112 1957 2084 112
Size: 1845 127 1996
Fragments arranged by size:
1996 1845 127
MspA1I CmG'CkG
Cuts at: 132 1079 1393 1753 2766 3011 132
Size: 947 314 360 1013 245 1089
Fragments arranged by size:
1089 1013 947 360 314 245
NaeI GCC'GGC
Cuts at: 2149 2149
Size: 3968
NarI GG'CG_CC
Cuts at: 1399 1647 1399
Size: 248 3720
NcoI C'CATG_G
Cuts at: 107 107
Size: 3968
NgoMIV G'CCGG_C
Cuts at: 2147 2147
Size: 3968
NspI r_CATG'y
Cuts at: 1021 1521 2428 1021
Size: 500 907 2561
Fragments arranged by size:
2561 907 500
PciI A'CATG_T
Cuts at: 2424 2424
Size: 3968
PfoI T'CCnGG_A
Cuts at: 281 281
Size: 3968
PmeI GTTT'AAAC
Cuts at: 967 967
Size: 3968
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3107 3139 3107
Size: 32 3936
PpuMI rG'GwC_Cy
Cuts at: 324 324
Size: 3968
PstI C_TGCA'G
Cuts at: 537 1015 1700 537
Size: 478 685 2805
Fragments arranged by size:
2805 685 478
PvuI CG_AT'CG
Cuts at: 104 104
Size: 3968
PvuII CAG'CTG
Cuts at: 1393 1753 1393
Size: 360 3608
RsrII CG'GwC_CG
Cuts at: 2163 2163
Size: 3968
SacI G_AGCT'C
Cuts at: 982 982
Size: 3968
SacII CC_GC'GG
Cuts at: 133 133
Size: 3968
SalI G'TCGA_C
Cuts at: 1005 1005
Size: 3968
SapI GCTCTTCn'nnn_
Cuts at: 1991 2201 1991
Size: 210 3758
SbfI CC_TGCA'GG
Cuts at: 1015 1015
Size: 3968
ScaI AGT'ACT
Cuts at: 742 3485 742
Size: 2743 1225
SfcI C'TryA_G
Cuts at: 33 144 353 533 710 1011 1696 2689 2880
Size: 111 209 180 177 301 685 993 191
Cuts at: 2880 33
Size: 1121
Fragments arranged by size:
1121 993 685 301 209 191 180 177
111
SfiI GGCCn_nnn'nGGCC
Cuts at: 665 665
Size: 3968
SfoI GGC'GCC
Cuts at: 1400 1648 1400
Size: 248 3720
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 3968
SmaI CCC'GGG
Cuts at: 990 990
Size: 3968
SmlI C'TyrA_G
Cuts at: 211 600 1123 2038 2530 2792 211
Size: 389 523 915 492 262 1387
Fragments arranged by size:
1387 915 523 492 389 262
SpeI A'CTAG_T
Cuts at: 67 568 67
Size: 501 3467
SphI G_CATG'C
Cuts at: 1021 1521 1021
Size: 500 3468
StuI AGG'CCT
Cuts at: 659 719 659
Size: 60 3908
StyI C'CwwG_G
Cuts at: 107 1102 1456 107
Size: 995 354 2619
Fragments arranged by size:
2619 995 354
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 363 1551 1684 2143 2352 363
Size: 1188 133 459 209 1979
Fragments arranged by size:
1979 1188 459 209 133
TatI w'GTAC_w
Cuts at: 740 3483 740
Size: 2743 1225
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3485 3517 3485
Size: 32 3936
Tth111I GACn'n_nGTC
Cuts at: 1765 1765
Size: 3968
XbaI T'CTAG_A
Cuts at: 999 999
Size: 3968
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1463 1463
Size: 3968
XmaI C'CCGG_G
Cuts at: 988 988
Size: 3968
XmnI GAAnn'nnTTC
Cuts at: 933 933
Size: 3968
Enzymes that do cut:
AccI Acc65I AclI AcuI AflII AflIII AgeI AlwNI
ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII
BbeI BciVI BclI BglI BglII BlpI Bme1580I BmrI
BplI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BseRI
BseYI BsgI BsiEI BsiHKAI Bsp1286I BspEI BspHI BspMI
BsrBI BsrDI BsrFI BssSI BstAPI BstBI BstYI BstZ17I
BtgI BtgZI BtsI DraI DraIII DrdI EaeI EagI
EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI
FspI HaeII Hin4I HincII KasI KpnI MluI MmeI
MscI MslI MspA1I NaeI NarI NcoI NgoMIV NspI
PciI PfoI PmeI PpiI PpuMI PstI PvuI PvuII
RsrII SacI SacII SalI SapI SbfI ScaI SfcI
SfiI SfoI SgfI SmaI SmlI SpeI SphI StuI
StyI TaqII TatI TstI Tth111I XbaI XcmI XmaI
XmnI
Enzymes that do not cut:
AarI AatII AfeI AhdI AleI AlfI AloI ApaI
AscI AvrII BaeI BbsI BbvCI BcgI BfrBI BmgBI
BmtI BpmI Bpu10I BsaXI BsiWI BsmI BsmBI BsrGI
BssHII BstEII BstXI Bsu36I ClaI CspCI EcoRV FseI
FspAI HindIII HpaI MfeI NdeI NheI NotI NruI
NsiI PacI PasI PflMI PmlI PshAI PsiI PspOMI
PspXI PsrI SanDI SexAI SgrAI SnaBI SrfI SspI
SwaI XhoI ZraI