Restriction map for
pF1KE0139
(Circular) (Six-base) MAPSORT of: pF1KE0139.seq Check: 5843 from: 1 to: 3653
pF1KE0139 3653 bp
With 182 enzymes: SgfI *
April 24, 2009 13:11 ..
AccI GT'mk_AC
Cuts at: 542 691 542
Size: 149 3504
Acc65I G'GTAC_C
Cuts at: 669 669
Size: 3653
AclI AA'CG_TT
Cuts at: 3493 3493
Size: 3653
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 230 1478 1910 2657 2925 230
Size: 1248 432 747 268 958
Fragments arranged by size:
1248 958 747 432 268
AflIII A'CryG_T
Cuts at: 1005 2109 3086 1005
Size: 1104 977 1572
Fragments arranged by size:
1572 1104 977
AgeI A'CCGG_T
Cuts at: 2041 2041
Size: 3653
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 528 528
Size: 3653
AlwNI CAG_nnn'CTG
Cuts at: 958 2525 958
Size: 1567 2086
ApaLI G'TGCA_C
Cuts at: 2423 2423
Size: 3653
ApoI r'AATT_y
Cuts at: 247 657 3006 3262 247
Size: 410 2349 256 638
Fragments arranged by size:
2349 638 410 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 3653
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 3653
AvaI C'yCGr_G
Cuts at: 673 673
Size: 3653
BamHI G'GATC_C
Cuts at: 678 678
Size: 3653
BanI G'GyrC_C
Cuts at: 669 1083 1331 1366 669
Size: 414 248 35 2956
Fragments arranged by size:
2956 414 248 35
BanII G_rGCy'C
Cuts at: 667 1697 667
Size: 1030 2623
BbeI G_GCGC'C
Cuts at: 1087 1335 1087
Size: 248 3405
BciVI GTATCCnnnnn_n'
Cuts at: 1551 2312 3653 1551
Size: 761 1341 1551
Fragments arranged by size:
1551 1341 761
BclI T'GATC_A
Cuts at: 1173 1173
Size: 3653
BglII A'GATC_T
Cuts at: 1168 3205 1168
Size: 2037 1616
BlpI GC'TnA_GC
Cuts at: 765 765
Size: 3653
Bme1580I G_kGCm'C
Cuts at: 1278 1371 2427 1278
Size: 93 1056 2504
Fragments arranged by size:
2504 1056 93
BmrI ACTGGGnnnn_n'
Cuts at: 865 946 1281 3425 865
Size: 81 335 2144 1093
Fragments arranged by size:
2144 1093 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 585 2020 585
Size: 1435 2218
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 295 295
Size: 3653
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 829 1708 2200 2498 829
Size: 879 492 298 1984
Fragments arranged by size:
1984 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 3357 3357
Size: 3653
BsaAI yAC'GTr
Cuts at: 1006 1006
Size: 3653
BsaBI GATnn'nnATC
Cuts at: 6 1167 3204 6
Size: 1161 2037 455
Fragments arranged by size:
2037 1161 455
BsaHI Gr'CG_yC
Cuts at: 1084 1332 2034 3530 1084
Size: 248 702 1496 1207
Fragments arranged by size:
1496 1207 702 248
BsaWI w'CCGG_w
Cuts at: 257 842 1363 2041 2315 2462 2873 257
Size: 585 521 678 274 147 411 1037
Fragments arranged by size:
1037 678 585 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 268 298 268
Size: 30 3623
BseYI C'CCAG_C
Cuts at: 1078 1836 2413 2909 1078
Size: 758 577 496 1822
Fragments arranged by size:
1822 758 577 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 183 183
Size: 3653
BsiEI CG_ry'CG
Cuts at: 104 121 1195 1241 2449 104
Size: 17 1074 46 1208 1308
Fragments arranged by size:
1308 1208 1074 46 17
BsiHKAI G_wGCw'C
Cuts at: 667 1445 1635 2427 667
Size: 778 190 792 1893
Fragments arranged by size:
1893 792 778 190
Bsp1286I G_dGCh'C
Cuts at: 667 1278 1371 1445 1635 1697 2427 667
Size: 611 93 74 190 62 730 1893
Fragments arranged by size:
1893 730 611 190 93 74 62
BspEI T'CCGG_A
Cuts at: 2873 2873
Size: 3653
BspHI T'CATG_A
Cuts at: 2829 2829
Size: 3653
BspMI ACCTGCnnnn'nnnn_
Cuts at: 155 703 917 1219 1600 155
Size: 548 214 302 381 2208
Fragments arranged by size:
2208 548 381 302 214
BsrBI CCG'CTC
Cuts at: 50 303 1945 1999 3483 3648 50
Size: 253 1642 54 1484 165 55
Fragments arranged by size:
1642 1484 253 165 55 54
BsrDI GCAATG_nn'
Cuts at: 1565 1565
Size: 3653
BsrFI r'CCGG_y
Cuts at: 195 1651 1832 2041 195
Size: 1456 181 209 1807
Fragments arranged by size:
1807 1456 209 181
BssSI C'ACGA_G
Cuts at: 428 1924 2282 428
Size: 1496 358 1799
Fragments arranged by size:
1799 1496 358
BstAPI GCAn_nnn'nTGC
Cuts at: 348 1030 348
Size: 682 2971
BstBI TT'CG_AA
Cuts at: 2014 2014
Size: 3653
BstXI CCAn_nnnn'nTGG
Cuts at: 471 557 471
Size: 86 3567
BstYI r'GATC_y
Cuts at: 1 678 1145 1168 1503 1749 2761 2847 2859
Size: 677 467 23 335 246 1012 86 12
Cuts at: 2859 3205 1
Size: 346 449
Fragments arranged by size:
1012 677 467 449 346 335 246 86
23 12
BtgI C'CryG_G
Cuts at: 107 556 107
Size: 449 3204
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 1603 1784 1947 1603
Size: 181 163 3309
Fragments arranged by size:
3309 181 163
BtsI GCAGTG_nn'
Cuts at: 896 896
Size: 3653
DraI TTT'AAA
Cuts at: 652 652
Size: 3653
DrdI GACnn_nn'nnGTC
Cuts at: 1359 2217 1359
Size: 858 2795
EaeI y'GGCC_r
Cuts at: 1238 1412 1803 1830 3175 1238
Size: 174 391 27 1345 1716
Fragments arranged by size:
1716 1345 391 174 27
EagI C'GGCC_G
Cuts at: 1238 1238
Size: 3653
EarI CTCTTCn'nnn_
Cuts at: 1676 1886 1676
Size: 210 3443
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 327 1861 2171 2317 3463 327
Size: 1534 310 146 1146 517
Fragments arranged by size:
1534 1146 517 310 146
EcoICRI GAG'CTC
Cuts at: 665 665
Size: 3653
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 230 295 1478 1910 2657 2925 230
Size: 65 1183 432 747 268 958
Fragments arranged by size:
1183 958 747 432 268 65
EcoO109I rG'GnC_Cy
Cuts at: 792 792
Size: 3653
EcoRI G'AATT_C
Cuts at: 247 657 247
Size: 410 3243
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 1672 1672
Size: 3653
FspI TGC'GCA
Cuts at: 1434 1434
Size: 3653
HaeII r_GCGC'y
Cuts at: 115 1087 1335 2357 3336 115
Size: 972 248 1022 979 432
Fragments arranged by size:
1022 979 972 432 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 268 300 354 386 1495 1525 1527 1557 268
Size: 32 54 32 1109 30 2 30 2364
Fragments arranged by size:
2364 1109 54 32 32 30 30 2
HincII GTy'rAC
Cuts at: 182 692 182
Size: 510 3143
KasI G'GCGC_C
Cuts at: 1083 1331 1083
Size: 248 3405
KpnI G_GTAC'C
Cuts at: 673 673
Size: 3653
MluI A'CGCG_T
Cuts at: 3086 3086
Size: 3653
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 2324 2508 2324
Size: 184 3469
MscI TGG'CCA
Cuts at: 1414 1414
Size: 3653
MslI CAynn'nnrTG
Cuts at: 398 510 1642 1769 398
Size: 112 1132 127 2282
Fragments arranged by size:
2282 1132 127 112
MspA1I CmG'CkG
Cuts at: 764 1078 1438 2451 2696 764
Size: 314 360 1013 245 1721
Fragments arranged by size:
1721 1013 360 314 245
NaeI GCC'GGC
Cuts at: 197 1834 197
Size: 1637 2016
NarI GG'CG_CC
Cuts at: 1084 1332 1084
Size: 248 3405
NcoI C'CATG_G
Cuts at: 107 107
Size: 3653
NgoMIV G'CCGG_C
Cuts at: 195 1832 195
Size: 1637 2016
NspI r_CATG'y
Cuts at: 706 1206 2113 706
Size: 500 907 2246
Fragments arranged by size:
2246 907 500
PasI CC'CwG_GG
Cuts at: 157 157
Size: 3653
PciI A'CATG_T
Cuts at: 2109 2109
Size: 3653
PmeI GTTT'AAAC
Cuts at: 652 652
Size: 3653
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2792 2824 2792
Size: 32 3621
PsiI TTA'TAA
Cuts at: 358 358
Size: 3653
PstI C_TGCA'G
Cuts at: 700 1385 700
Size: 685 2968
PvuI CG_AT'CG
Cuts at: 104 104
Size: 3653
PvuII CAG'CTG
Cuts at: 1078 1438 1078
Size: 360 3293
RsrII CG'GwC_CG
Cuts at: 1848 1848
Size: 3653
SacI G_AGCT'C
Cuts at: 667 667
Size: 3653
SalI G'TCGA_C
Cuts at: 690 690
Size: 3653
SapI GCTCTTCn'nnn_
Cuts at: 1676 1886 1676
Size: 210 3443
SbfI CC_TGCA'GG
Cuts at: 700 700
Size: 3653
ScaI AGT'ACT
Cuts at: 3170 3170
Size: 3653
SfcI C'TryA_G
Cuts at: 33 287 696 1381 2374 2565 33
Size: 254 409 685 993 191 1121
Fragments arranged by size:
1121 993 685 409 254 191
SfoI GGC'GCC
Cuts at: 1085 1333 1085
Size: 248 3405
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 3653
SmaI CCC'GGG
Cuts at: 675 675
Size: 3653
SmlI C'TyrA_G
Cuts at: 808 1723 2215 2477 808
Size: 915 492 262 1984
Fragments arranged by size:
1984 915 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 3653
SphI G_CATG'C
Cuts at: 706 1206 706
Size: 500 3153
StyI C'CwwG_G
Cuts at: 107 787 1141 107
Size: 680 354 2619
Fragments arranged by size:
2619 680 354
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 107 149 1236 1369 1828 2037 107
Size: 42 1087 133 459 209 1723
Fragments arranged by size:
1723 1087 459 209 133 42
TatI w'GTAC_w
Cuts at: 3168 3168
Size: 3653
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3170 3202 3170
Size: 32 3621
Tth111I GACn'n_nGTC
Cuts at: 1450 1450
Size: 3653
XbaI T'CTAG_A
Cuts at: 684 684
Size: 3653
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 471 1148 471
Size: 677 2976
XmaI C'CCGG_G
Cuts at: 673 673
Size: 3653
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AgeI AlfI AlwNI
ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII
BbeI BciVI BclI BglII BlpI Bme1580I BmrI BplI
BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI
BseYI BsgI BsiEI BsiHKAI Bsp1286I BspEI BspHI BspMI
BsrBI BsrDI BsrFI BssSI BstAPI BstBI BstXI BstYI
BtgI BtgZI BtsI DraI DrdI EaeI EagI EarI
EciI EcoICRI Eco57MI EcoO109I EcoRI FalI FspI HaeII
Hin4I HincII KasI KpnI MluI MmeI MscI MslI
MspA1I NaeI NarI NcoI NgoMIV NspI PasI PciI
PmeI PpiI PsiI PstI PvuI PvuII RsrII SacI
SalI SapI SbfI ScaI SfcI SfoI SgfI SmaI
SmlI SpeI SphI StyI TaqII TatI TstI Tth111I
XbaI XcmI XmaI
Enzymes that do not cut:
AarI AatII AfeI AflII AhdI AleI AloI ApaI
AscI AvrII BaeI BbsI BbvCI BcgI BfrBI BglI
BmgBI BmtI Bpu10I BseRI BsiWI BsmI BsmBI BsrGI
BssHII BstEII BstZ17I Bsu36I ClaI CspCI DraIII EcoNI
EcoRV FseI FspAI HindIII HpaI MfeI NdeI NheI
NotI NruI NsiI PacI PflMI PfoI PmlI PpuMI
PshAI PspOMI PspXI PsrI SacII SanDI SexAI SfiI
SgrAI SnaBI SrfI SspI StuI SwaI XhoI XmnI
ZraI