Restriction map for
pF1KE0136
(Circular) (Six-base) MAPSORT of: pF1KE0136.seq Check: 3466 from: 1 to: 4019
pF1KE0136 4019 bp
With 182 enzymes: SgfI *
April 24, 2009 13:10 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 210 210
Size: 4019
AatII G_ACGT'C
Cuts at: 473 473
Size: 4019
AccI GT'mk_AC
Cuts at: 1057 1057
Size: 4019
Acc65I G'GTAC_C
Cuts at: 1035 1035
Size: 4019
AclI AA'CG_TT
Cuts at: 3859 3859
Size: 4019
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 943 1844 2276 3023 3291 943
Size: 901 432 747 268 1671
Fragments arranged by size:
1671 901 747 432 268
AfeI AGC'GCT
Cuts at: 811 811
Size: 4019
AflIII A'CryG_T
Cuts at: 449 1371 2475 3452 449
Size: 922 1104 977 1016
Fragments arranged by size:
1104 1016 977 922
AgeI A'CCGG_T
Cuts at: 2407 2407
Size: 4019
AleI CACnn'nnGTG
Cuts at: 757 757
Size: 4019
AlwNI CAG_nnn'CTG
Cuts at: 192 438 1324 2891 192
Size: 246 886 1567 1320
Fragments arranged by size:
1567 1320 886 246
ApaI G_GGCC'C
Cuts at: 156 350 156
Size: 194 3825
ApaLI G'TGCA_C
Cuts at: 2789 2789
Size: 4019
ApoI r'AATT_y
Cuts at: 1023 3372 3628 1023
Size: 2349 256 1414
Fragments arranged by size:
2349 1414 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 4019
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4019
AvaI C'yCGr_G
Cuts at: 479 1039 479
Size: 560 3459
BamHI G'GATC_C
Cuts at: 1044 1044
Size: 4019
BanI G'GyrC_C
Cuts at: 1035 1449 1697 1732 1035
Size: 414 248 35 3322
Fragments arranged by size:
3322 414 248 35
BanII G_rGCy'C
Cuts at: 156 350 1033 2063 156
Size: 194 683 1030 2112
Fragments arranged by size:
2112 1030 683 194
BbeI G_GCGC'C
Cuts at: 1453 1701 1453
Size: 248 3771
BbvCI CC'TCA_GC
Cuts at: 995 995
Size: 4019
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 90 124 90
Size: 34 3985
BciVI GTATCCnnnnn_n'
Cuts at: 236 1917 2678 4019 236
Size: 1681 761 1341 236
Fragments arranged by size:
1681 1341 761 236
BclI T'GATC_A
Cuts at: 1539 1539
Size: 4019
BglI GCCn_nnn'nGGC
Cuts at: 151 208 151
Size: 57 3962
BglII A'GATC_T
Cuts at: 1534 3571 1534
Size: 2037 1982
BlpI GC'TnA_GC
Cuts at: 928 1131 928
Size: 203 3816
Bme1580I G_kGCm'C
Cuts at: 156 350 1006 1644 1737 2793 156
Size: 194 656 638 93 1056 1382
Fragments arranged by size:
1382 1056 656 638 194 93
BmgBI CAC'GTC
Cuts at: 636 636
Size: 4019
BmrI ACTGGGnnnn_n'
Cuts at: 492 738 1231 1312 1647 3791 492
Size: 246 493 81 335 2144 720
Fragments arranged by size:
2144 720 493 335 246 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2386 2386
Size: 4019
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 625 721 625
Size: 96 3923
Bpu10I CC'TnA_GC
Cuts at: 361 995 361
Size: 634 3385
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1195 2074 2566 2864 1195
Size: 879 492 298 2350
Fragments arranged by size:
2350 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 615 3723 615
Size: 3108 911
BsaAI yAC'GTr
Cuts at: 450 1372 450
Size: 922 3097
BsaBI GATnn'nnATC
Cuts at: 6 1533 3570 6
Size: 1527 2037 455
Fragments arranged by size:
2037 1527 455
BsaHI Gr'CG_yC
Cuts at: 470 1450 1698 2400 3896 470
Size: 980 248 702 1496 593
Fragments arranged by size:
1496 980 702 593 248
BsaWI w'CCGG_w
Cuts at: 1208 1729 2407 2681 2828 3239 1208
Size: 521 678 274 147 411 1988
Fragments arranged by size:
1988 678 521 411 274 147
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 118 889 892 118
Size: 771 3 3245
Fragments arranged by size:
3245 771 3
BseYI C'CCAG_C
Cuts at: 185 371 375 511 526 614 1444 2202 2779
Size: 186 4 136 15 88 830 758 577
Cuts at: 2779 3275 185
Size: 496 929
Fragments arranged by size:
929 830 758 577 496 186 136 88
15 4
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 107 532 762 784 811 107
Size: 425 230 22 27 3315
Fragments arranged by size:
3315 425 230 27 22
BsiEI CG_ry'CG
Cuts at: 104 1561 1607 2815 104
Size: 1457 46 1208 1308
Fragments arranged by size:
1457 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 1033 1811 2001 2793 1033
Size: 778 190 792 2259
Fragments arranged by size:
2259 792 778 190
BsmBI CGTCTCn'nnnn_
Cuts at: 391 391
Size: 4019
Bsp1286I G_dGCh'C
Cuts at: 156 350 1006 1033 1644 1737 1811 2001 2063
Size: 194 656 27 611 93 74 190 62
Cuts at: 2063 2793 156
Size: 730 1382
Fragments arranged by size:
1382 730 656 611 194 190 93 74
62 27
BspEI T'CCGG_A
Cuts at: 3239 3239
Size: 4019
BspHI T'CATG_A
Cuts at: 3195 3195
Size: 4019
BspMI ACCTGCnnnn'nnnn_
Cuts at: 210 1069 1283 1585 1966 210
Size: 859 214 302 381 2263
Fragments arranged by size:
2263 859 381 302 214
BsrBI CCG'CTC
Cuts at: 50 2311 2365 3849 4014 50
Size: 2261 54 1484 165 55
Fragments arranged by size:
2261 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 522 1931 522
Size: 1409 2610
BsrFI r'CCGG_y
Cuts at: 2017 2198 2407 2017
Size: 181 209 3629
Fragments arranged by size:
3629 209 181
BsrGI T'GTAC_A
Cuts at: 452 452
Size: 4019
BssSI C'ACGA_G
Cuts at: 855 2290 2648 855
Size: 1435 358 2226
Fragments arranged by size:
2226 1435 358
BstAPI GCAn_nnn'nTGC
Cuts at: 776 1396 776
Size: 620 3399
BstBI TT'CG_AA
Cuts at: 2380 2380
Size: 4019
BstYI r'GATC_y
Cuts at: 1 1044 1511 1534 1869 2115 3127 3213 3225
Size: 1043 467 23 335 246 1012 86 12
Cuts at: 3225 3571 1
Size: 346 449
Fragments arranged by size:
1043 1012 467 449 346 335 246 86
23 12
Bsu36I CC'TnA_GG
Cuts at: 560 560
Size: 4019
BtgI C'CryG_G
Cuts at: 908 908
Size: 4019
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 1969 2150 2313 1969
Size: 181 163 3675
Fragments arranged by size:
3675 181 163
BtsI GCAGTG_nn'
Cuts at: 774 830 1262 774
Size: 56 432 3531
Fragments arranged by size:
3531 432 56
ClaI AT'CG_AT
Cuts at: 330 330
Size: 4019
DraI TTT'AAA
Cuts at: 1018 1018
Size: 4019
DraIII CAC_nnn'GTG
Cuts at: 732 732
Size: 4019
DrdI GACnn_nn'nnGTC
Cuts at: 1725 2583 1725
Size: 858 3161
EaeI y'GGCC_r
Cuts at: 143 200 209 1604 1778 2169 2196 3541 143
Size: 57 9 1395 174 391 27 1345 621
Fragments arranged by size:
1395 1345 621 391 174 57 27 9
EagI C'GGCC_G
Cuts at: 1604 1604
Size: 4019
EarI CTCTTCn'nnn_
Cuts at: 136 294 2042 2252 136
Size: 158 1748 210 1903
Fragments arranged by size:
1903 1748 210 158
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2227 2537 2683 3829 2227
Size: 310 146 1146 2417
Fragments arranged by size:
2417 1146 310 146
EcoICRI GAG'CTC
Cuts at: 1031 1031
Size: 4019
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 625 721 943 1844 2276 3023 3291 625
Size: 96 222 901 432 747 268 1353
Fragments arranged by size:
1353 901 747 432 268 222 96
EcoO109I rG'GnC_Cy
Cuts at: 152 347 435 882 1158 152
Size: 195 88 447 276 3013
Fragments arranged by size:
3013 447 276 195 88
EcoRI G'AATT_C
Cuts at: 1023 1023
Size: 4019
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2038 2038
Size: 4019
FspI TGC'GCA
Cuts at: 1800 1800
Size: 4019
HaeII r_GCGC'y
Cuts at: 302 690 813 1453 1701 2723 3702 302
Size: 388 123 640 248 1022 979 619
Fragments arranged by size:
1022 979 640 619 388 248 123
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 234 266 1861 1891 1893 1923 234
Size: 32 1595 30 2 30 2330
Fragments arranged by size:
2330 1595 32 30 30 2
HincII GTy'rAC
Cuts at: 1058 1058
Size: 4019
KasI G'GCGC_C
Cuts at: 1449 1697 1449
Size: 248 3771
KpnI G_GTAC'C
Cuts at: 1039 1039
Size: 4019
MluI A'CGCG_T
Cuts at: 3452 3452
Size: 4019
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 2690 2874 2690
Size: 184 3835
MscI TGG'CCA
Cuts at: 145 202 211 1780 145
Size: 57 9 1569 2384
Fragments arranged by size:
2384 1569 57 9
MslI CAynn'nnrTG
Cuts at: 265 391 757 2008 2135 265
Size: 126 366 1251 127 2149
Fragments arranged by size:
2149 1251 366 127 126
MspA1I CmG'CkG
Cuts at: 189 375 546 999 1130 1444 1804 2817 3062
Size: 186 171 453 131 314 360 1013 245
Cuts at: 3062 189
Size: 1146
Fragments arranged by size:
1146 1013 453 360 314 245 186 171
131
NaeI GCC'GGC
Cuts at: 2200 2200
Size: 4019
NarI GG'CG_CC
Cuts at: 1450 1698 1450
Size: 248 3771
NgoMIV G'CCGG_C
Cuts at: 2198 2198
Size: 4019
NspI r_CATG'y
Cuts at: 1072 1572 2479 1072
Size: 500 907 2612
Fragments arranged by size:
2612 907 500
PciI A'CATG_T
Cuts at: 2475 2475
Size: 4019
PmeI GTTT'AAAC
Cuts at: 1018 1018
Size: 4019
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3158 3190 3158
Size: 32 3987
PpuMI rG'GwC_Cy
Cuts at: 435 435
Size: 4019
PspOMI G'GGCC_C
Cuts at: 152 346 152
Size: 194 3825
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 574 606 574
Size: 32 3987
PstI C_TGCA'G
Cuts at: 275 1066 1751 275
Size: 791 685 2543
Fragments arranged by size:
2543 791 685
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4019
PvuII CAG'CTG
Cuts at: 375 999 1444 1804 375
Size: 624 445 360 2590
Fragments arranged by size:
2590 624 445 360
RsrII CG'GwC_CG
Cuts at: 2214 2214
Size: 4019
SacI G_AGCT'C
Cuts at: 1033 1033
Size: 4019
SalI G'TCGA_C
Cuts at: 1056 1056
Size: 4019
SapI GCTCTTCn'nnn_
Cuts at: 2042 2252 2042
Size: 210 3809
SbfI CC_TGCA'GG
Cuts at: 1066 1066
Size: 4019
ScaI AGT'ACT
Cuts at: 3536 3536
Size: 4019
SfcI C'TryA_G
Cuts at: 33 271 351 1062 1747 2740 2931 33
Size: 238 80 711 685 993 191 1121
Fragments arranged by size:
1121 993 711 685 238 191 80
SfiI GGCCn_nnn'nGGCC
Cuts at: 151 208 151
Size: 57 3962
SfoI GGC'GCC
Cuts at: 1451 1699 1451
Size: 248 3771
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4019
SmaI CCC'GGG
Cuts at: 481 1041 481
Size: 560 3459
SmlI C'TyrA_G
Cuts at: 1174 2089 2581 2843 1174
Size: 915 492 262 2350
Fragments arranged by size:
2350 915 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4019
SphI G_CATG'C
Cuts at: 1072 1572 1072
Size: 500 3519
StuI AGG'CCT
Cuts at: 160 160
Size: 4019
StyI C'CwwG_G
Cuts at: 203 1153 1507 203
Size: 950 354 2715
Fragments arranged by size:
2715 950 354
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 407 720 787 1602 1735 2194 2403 407
Size: 313 67 815 133 459 209 2023
Fragments arranged by size:
2023 815 459 313 209 133 67
TatI w'GTAC_w
Cuts at: 407 452 3534 407
Size: 45 3082 892
Fragments arranged by size:
3082 892 45
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 116 148 929 961 3536 3568 116
Size: 32 781 32 2575 32 567
Fragments arranged by size:
2575 781 567 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 790 1816 790
Size: 1026 2993
XbaI T'CTAG_A
Cuts at: 1050 1050
Size: 4019
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 534 687 725 1514 534
Size: 153 38 789 3039
Fragments arranged by size:
3039 789 153 38
XmaI C'CCGG_G
Cuts at: 479 1039 479
Size: 560 3459
ZraI GAC'GTC
Cuts at: 471 471
Size: 4019
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AfeI AflIII
AgeI AleI AlwNI ApaI ApaLI ApoI AseI AsiSI
AvaI BamHI BanI BanII BbeI BbvCI BcgI BciVI
BclI BglI BglII BlpI Bme1580I BmgBI BmrI BplI
BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BseRI BseYI BsgI BsiEI BsiHKAI BsmBI Bsp1286I BspEI
BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssSI BstAPI
BstBI BstYI Bsu36I BtgI BtgZI BtsI ClaI DraI
DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI
EcoO109I EcoRI FalI FspI HaeII Hin4I HincII KasI
KpnI MluI MmeI MscI MslI MspA1I NaeI NarI
NgoMIV NspI PciI PmeI PpiI PpuMI PspOMI PsrI
PstI PvuI PvuII RsrII SacI SalI SapI SbfI
ScaI SfcI SfiI SfoI SgfI SmaI SmlI SpeI
SphI StuI StyI TaqII TatI TstI Tth111I XbaI
XcmI XmaI ZraI
Enzymes that do not cut:
AflII AhdI AlfI AloI AscI AvrII BaeI BbsI
BfrBI BmtI BsaXI BsiWI BsmI BssHII BstEII BstXI
BstZ17I CspCI EcoNI EcoRV FseI FspAI HindIII HpaI
MfeI NcoI NdeI NheI NotI NruI NsiI PacI
PasI PflMI PfoI PmlI PshAI PsiI PspXI SacII
SanDI SexAI SgrAI SnaBI SrfI SspI SwaI XhoI
XmnI