Restriction map for
pF1KE0129
(Circular) (Six-base) MAPSORT of: pF1KE0129.seq Check: 9781 from: 1 to: 3857
pF1KE0129 3857 bp
With 182 enzymes: SgfI *
April 24, 2009 13:10 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 208 377 753 208
Size: 169 376 3312
Fragments arranged by size:
3312 376 169
AccI GT'mk_AC
Cuts at: 713 895 713
Size: 182 3675
Acc65I G'GTAC_C
Cuts at: 873 873
Size: 3857
AclI AA'CG_TT
Cuts at: 3697 3697
Size: 3857
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1682 2114 2861 3129 1682
Size: 432 747 268 2410
Fragments arranged by size:
2410 747 432 268
AflIII A'CryG_T
Cuts at: 1209 2313 3290 1209
Size: 1104 977 1776
Fragments arranged by size:
1776 1104 977
AgeI A'CCGG_T
Cuts at: 2245 2245
Size: 3857
AlwNI CAG_nnn'CTG
Cuts at: 369 1162 2729 369
Size: 793 1567 1497
Fragments arranged by size:
1567 1497 793
ApaI G_GGCC'C
Cuts at: 154 624 154
Size: 470 3387
ApaLI G'TGCA_C
Cuts at: 184 2627 184
Size: 2443 1414
ApoI r'AATT_y
Cuts at: 861 3210 3466 861
Size: 2349 256 1252
Fragments arranged by size:
2349 1252 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 3857
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 3857
AvaI C'yCGr_G
Cuts at: 877 877
Size: 3857
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 520 553 520
Size: 33 3824
BamHI G'GATC_C
Cuts at: 882 882
Size: 3857
BanI G'GyrC_C
Cuts at: 168 726 873 1287 1535 1570 168
Size: 558 147 414 248 35 2455
Fragments arranged by size:
2455 558 414 248 147 35
BanII G_rGCy'C
Cuts at: 154 428 624 871 1901 154
Size: 274 196 247 1030 2110
Fragments arranged by size:
2110 1030 274 247 196
BbeI G_GCGC'C
Cuts at: 172 1291 1539 172
Size: 1119 248 2490
Fragments arranged by size:
2490 1119 248
BbsI GAAGACnn'nnnn_
Cuts at: 323 323
Size: 3857
BciVI GTATCCnnnnn_n'
Cuts at: 1755 2516 3857 1755
Size: 761 1341 1755
Fragments arranged by size:
1755 1341 761
BclI T'GATC_A
Cuts at: 1377 1377
Size: 3857
BglI GCCn_nnn'nGGC
Cuts at: 759 759
Size: 3857
BglII A'GATC_T
Cuts at: 1372 3409 1372
Size: 2037 1820
BlpI GC'TnA_GC
Cuts at: 969 969
Size: 3857
Bme1580I G_kGCm'C
Cuts at: 154 188 624 731 1482 1575 2631 154
Size: 34 436 107 751 93 1056 1380
Fragments arranged by size:
1380 1056 751 436 107 93 34
BmrI ACTGGGnnnn_n'
Cuts at: 1069 1150 1485 3629 1069
Size: 81 335 2144 1297
Fragments arranged by size:
2144 1297 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2224 2224
Size: 3857
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 547 547
Size: 3857
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1033 1912 2404 2702 1033
Size: 879 492 298 2188
Fragments arranged by size:
2188 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 283 519 3561 283
Size: 236 3042 579
Fragments arranged by size:
3042 579 236
BsaAI yAC'GTr
Cuts at: 1210 1210
Size: 3857
BsaBI GATnn'nnATC
Cuts at: 6 1371 3408 6
Size: 1365 2037 455
Fragments arranged by size:
2037 1365 455
BsaHI Gr'CG_yC
Cuts at: 169 302 1288 1536 2238 3734 169
Size: 133 986 248 702 1496 292
Fragments arranged by size:
1496 986 702 292 248 133
BsaWI w'CCGG_w
Cuts at: 1046 1567 2245 2519 2666 3077 1046
Size: 521 678 274 147 411 1826
Fragments arranged by size:
1826 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 710 740 710
Size: 30 3827
BseYI C'CCAG_C
Cuts at: 671 1282 2040 2617 3113 671
Size: 611 758 577 496 1415
Fragments arranged by size:
1415 758 611 577 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 493 493
Size: 3857
BsiEI CG_ry'CG
Cuts at: 104 1399 1445 2653 104
Size: 1295 46 1208 1308
Fragments arranged by size:
1308 1295 1208 46
BsiHKAI G_wGCw'C
Cuts at: 188 695 871 1649 1839 2631 188
Size: 507 176 778 190 792 1414
Fragments arranged by size:
1414 792 778 507 190 176
Bsp1286I G_dGCh'C
Cuts at: 154 188 428 624 695 731 871 1482 1575
Size: 34 240 196 71 36 140 611 93
Cuts at: 1575 1649 1839 1901 2631 154
Size: 74 190 62 730 1380
Fragments arranged by size:
1380 730 611 240 196 190 140 93
74 71 62 36 34
BspEI T'CCGG_A
Cuts at: 3077 3077
Size: 3857
BspHI T'CATG_A
Cuts at: 3033 3033
Size: 3857
BspMI ACCTGCnnnn'nnnn_
Cuts at: 208 377 548 699 753 907 1121 1423 1804
Size: 169 171 151 54 154 214 302 381
Cuts at: 1804 208
Size: 2261
Fragments arranged by size:
2261 381 302 214 171 169 154 151
54
BsrBI CCG'CTC
Cuts at: 50 2149 2203 3687 3852 50
Size: 2099 54 1484 165 55
Fragments arranged by size:
2099 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 248 1769 248
Size: 1521 2336
BsrFI r'CCGG_y
Cuts at: 165 346 1855 2036 2245 165
Size: 181 1509 181 209 1777
Fragments arranged by size:
1777 1509 209 181 181
BssSI C'ACGA_G
Cuts at: 377 2128 2486 377
Size: 1751 358 1748
Fragments arranged by size:
1751 1748 358
BstAPI GCAn_nnn'nTGC
Cuts at: 369 1234 369
Size: 865 2992
BstBI TT'CG_AA
Cuts at: 2218 2218
Size: 3857
BstYI r'GATC_y
Cuts at: 1 845 882 1349 1372 1707 1953 2965 3051
Size: 844 37 467 23 335 246 1012 86
Cuts at: 3051 3063 3409 1
Size: 12 346 449
Fragments arranged by size:
1012 844 467 449 346 335 246 86
37 23 12
BtgI C'CryG_G
Cuts at: 107 642 683 754 833 107
Size: 535 41 71 79 3131
Fragments arranged by size:
3131 535 79 71 41
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 663 1807 1988 2151 663
Size: 1144 181 163 2369
Fragments arranged by size:
2369 1144 181 163
BtsI GCAGTG_nn'
Cuts at: 254 481 1100 254
Size: 227 619 3011
Fragments arranged by size:
3011 619 227
DraI TTT'AAA
Cuts at: 856 856
Size: 3857
DrdI GACnn_nn'nnGTC
Cuts at: 1563 2421 1563
Size: 858 2999
EaeI y'GGCC_r
Cuts at: 344 1442 1616 2007 2034 3379 344
Size: 1098 174 391 27 1345 822
Fragments arranged by size:
1345 1098 822 391 174 27
EagI C'GGCC_G
Cuts at: 1442 1442
Size: 3857
EarI CTCTTCn'nnn_
Cuts at: 353 1880 2090 353
Size: 1527 210 2120
Fragments arranged by size:
2120 1527 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 143 2065 2375 2521 3667 143
Size: 1922 310 146 1146 333
Fragments arranged by size:
1922 1146 333 310 146
EcoICRI GAG'CTC
Cuts at: 869 869
Size: 3857
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 547 1682 2114 2861 3129 547
Size: 1135 432 747 268 1275
Fragments arranged by size:
1275 1135 747 432 268
EcoO109I rG'GnC_Cy
Cuts at: 136 150 621 996 136
Size: 14 471 375 2997
Fragments arranged by size:
2997 471 375 14
EcoRI G'AATT_C
Cuts at: 861 861
Size: 3857
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 1876 1876
Size: 3857
FspI TGC'GCA
Cuts at: 1638 1638
Size: 3857
HaeII r_GCGC'y
Cuts at: 172 652 1291 1539 2561 3540 172
Size: 480 639 248 1022 979 489
Fragments arranged by size:
1022 979 639 489 480 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1699 1729 1731 1761 1699
Size: 30 2 30 3795
Fragments arranged by size:
3795 30 30 2
HincII GTy'rAC
Cuts at: 896 896
Size: 3857
KasI G'GCGC_C
Cuts at: 168 1287 1535 168
Size: 1119 248 2490
Fragments arranged by size:
2490 1119 248
KpnI G_GTAC'C
Cuts at: 877 877
Size: 3857
MluI A'CGCG_T
Cuts at: 3290 3290
Size: 3857
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 422 2528 2712 422
Size: 2106 184 1567
Fragments arranged by size:
2106 1567 184
MscI TGG'CCA
Cuts at: 1618 1618
Size: 3857
MslI CAynn'nnrTG
Cuts at: 391 682 1846 1973 391
Size: 291 1164 127 2275
Fragments arranged by size:
2275 1164 291 127
MspA1I CmG'CkG
Cuts at: 587 594 968 1282 1642 2655 2900 587
Size: 7 374 314 360 1013 245 1544
Fragments arranged by size:
1544 1013 374 360 314 245 7
NaeI GCC'GGC
Cuts at: 167 348 2038 167
Size: 181 1690 1986
Fragments arranged by size:
1986 1690 181
NarI GG'CG_CC
Cuts at: 169 1288 1536 169
Size: 1119 248 2490
Fragments arranged by size:
2490 1119 248
NcoI C'CATG_G
Cuts at: 107 642 833 107
Size: 535 191 3131
Fragments arranged by size:
3131 535 191
NgoMIV G'CCGG_C
Cuts at: 165 346 2036 165
Size: 181 1690 1986
Fragments arranged by size:
1986 1690 181
NspI r_CATG'y
Cuts at: 910 1410 2317 910
Size: 500 907 2450
Fragments arranged by size:
2450 907 500
PciI A'CATG_T
Cuts at: 2313 2313
Size: 3857
PflMI CCAn_nnn'nTGG
Cuts at: 552 552
Size: 3857
PfoI T'CCnGG_A
Cuts at: 458 458
Size: 3857
PmeI GTTT'AAAC
Cuts at: 856 856
Size: 3857
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2996 3028 2996
Size: 32 3825
PspOMI G'GGCC_C
Cuts at: 150 620 150
Size: 470 3387
PstI C_TGCA'G
Cuts at: 144 710 904 1589 144
Size: 566 194 685 2412
Fragments arranged by size:
2412 685 566 194
PvuI CG_AT'CG
Cuts at: 104 104
Size: 3857
PvuII CAG'CTG
Cuts at: 587 1282 1642 587
Size: 695 360 2802
Fragments arranged by size:
2802 695 360
RsrII CG'GwC_CG
Cuts at: 2052 2052
Size: 3857
SacI G_AGCT'C
Cuts at: 871 871
Size: 3857
SalI G'TCGA_C
Cuts at: 894 894
Size: 3857
SapI GCTCTTCn'nnn_
Cuts at: 353 1880 2090 353
Size: 1527 210 2120
Fragments arranged by size:
2120 1527 210
SbfI CC_TGCA'GG
Cuts at: 710 904 710
Size: 194 3663
ScaI AGT'ACT
Cuts at: 3374 3374
Size: 3857
SexAI A'CCwGG_T
Cuts at: 551 551
Size: 3857
SfcI C'TryA_G
Cuts at: 33 140 706 900 1585 2578 2769 33
Size: 107 566 194 685 993 191 1121
Fragments arranged by size:
1121 993 685 566 194 191 107
SfoI GGC'GCC
Cuts at: 170 1289 1537 170
Size: 1119 248 2490
Fragments arranged by size:
2490 1119 248
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 3857
SmaI CCC'GGG
Cuts at: 879 879
Size: 3857
SmlI C'TyrA_G
Cuts at: 1012 1927 2419 2681 1012
Size: 915 492 262 2188
Fragments arranged by size:
2188 915 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 3857
SphI G_CATG'C
Cuts at: 910 1410 910
Size: 500 3357
SspI AAT'ATT
Cuts at: 804 804
Size: 3857
StyI C'CwwG_G
Cuts at: 107 642 833 991 1345 107
Size: 535 191 158 354 2619
Fragments arranged by size:
2619 535 354 191 158
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1440 1573 2032 2241 1440
Size: 133 459 209 3056
Fragments arranged by size:
3056 459 209 133
TatI w'GTAC_w
Cuts at: 3372 3372
Size: 3857
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 710 742 3374 3406 710
Size: 32 2632 32 1161
Fragments arranged by size:
2632 1161 32 32
Tth111I GACn'n_nGTC
Cuts at: 1654 1654
Size: 3857
XbaI T'CTAG_A
Cuts at: 888 888
Size: 3857
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1352 1352
Size: 3857
XmaI C'CCGG_G
Cuts at: 877 877
Size: 3857
Enzymes that do cut:
AarI AccI Acc65I AclI AcuI AflIII AgeI AlwNI
ApaI ApaLI ApoI AseI AsiSI AvaI BaeI BamHI
BanI BanII BbeI BbsI BciVI BclI BglI BglII
BlpI Bme1580I BmrI BplI BpmI BpuEI BsaI BsaAI
BsaBI BsaHI BsaWI BsaXI BseYI BsgI BsiEI BsiHKAI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BssSI
BstAPI BstBI BstYI BtgI BtgZI BtsI DraI DrdI
EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI
FalI FspI HaeII Hin4I HincII KasI KpnI MluI
MmeI MscI MslI MspA1I NaeI NarI NcoI NgoMIV
NspI PciI PflMI PfoI PmeI PpiI PspOMI PstI
PvuI PvuII RsrII SacI SalI SapI SbfI ScaI
SexAI SfcI SfoI SgfI SmaI SmlI SpeI SphI
SspI StyI TaqII TatI TstI Tth111I XbaI XcmI
XmaI
Enzymes that do not cut:
AatII AfeI AflII AhdI AleI AlfI AloI AscI
AvrII BbvCI BcgI BfrBI BmgBI BmtI Bpu10I BseRI
BsiWI BsmI BsmBI BsrGI BssHII BstEII BstXI BstZ17I
Bsu36I ClaI CspCI DraIII EcoNI EcoRV FseI FspAI
HindIII HpaI MfeI NdeI NheI NotI NruI NsiI
PacI PasI PmlI PpuMI PshAI PsiI PspXI PsrI
SacII SanDI SfiI SgrAI SnaBI SrfI StuI SwaI
XhoI XmnI ZraI