Restriction map for
pF1KE0112
(Circular) (Six-base) MAPSORT of: pF1KE0112.seq Check: 8111 from: 1 to: 3698
pF1KE0112 3698 bp
With 182 enzymes: SgfI *
April 24, 2009 13:10 ..
AccI GT'mk_AC
Cuts at: 736 736
Size: 3698
Acc65I G'GTAC_C
Cuts at: 714 714
Size: 3698
AclI AA'CG_TT
Cuts at: 3538 3538
Size: 3698
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1523 1955 2702 2970 1523
Size: 432 747 268 2251
Fragments arranged by size:
2251 747 432 268
AfeI AGC'GCT
Cuts at: 678 678
Size: 3698
AflIII A'CryG_T
Cuts at: 1050 2154 3131 1050
Size: 1104 977 1617
Fragments arranged by size:
1617 1104 977
AgeI A'CCGG_T
Cuts at: 2086 2086
Size: 3698
AlwNI CAG_nnn'CTG
Cuts at: 1003 2570 1003
Size: 1567 2131
ApaI G_GGCC'C
Cuts at: 620 620
Size: 3698
ApaLI G'TGCA_C
Cuts at: 2468 2468
Size: 3698
ApoI r'AATT_y
Cuts at: 379 702 3051 3307 379
Size: 323 2349 256 770
Fragments arranged by size:
2349 770 323 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 3698
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 3698
AvaI C'yCGr_G
Cuts at: 134 645 718 134
Size: 511 73 3114
Fragments arranged by size:
3114 511 73
BamHI G'GATC_C
Cuts at: 723 723
Size: 3698
BanI G'GyrC_C
Cuts at: 502 714 1128 1376 1411 502
Size: 212 414 248 35 2789
Fragments arranged by size:
2789 414 248 212 35
BanII G_rGCy'C
Cuts at: 124 620 712 1742 124
Size: 496 92 1030 2080
Fragments arranged by size:
2080 1030 496 92
BbeI G_GCGC'C
Cuts at: 1132 1380 1132
Size: 248 3450
BbvCI CC'TCA_GC
Cuts at: 329 329
Size: 3698
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 505 539 505
Size: 34 3664
BciVI GTATCCnnnnn_n'
Cuts at: 1596 2357 3698 1596
Size: 761 1341 1596
Fragments arranged by size:
1596 1341 761
BclI T'GATC_A
Cuts at: 1218 1218
Size: 3698
BglI GCCn_nnn'nGGC
Cuts at: 157 580 157
Size: 423 3275
BglII A'GATC_T
Cuts at: 1213 3250 1213
Size: 2037 1661
BlpI GC'TnA_GC
Cuts at: 680 810 680
Size: 130 3568
Bme1580I G_kGCm'C
Cuts at: 523 620 1323 1416 2472 523
Size: 97 703 93 1056 1749
Fragments arranged by size:
1749 1056 703 97 93
BmgBI CAC'GTC
Cuts at: 144 144
Size: 3698
BmrI ACTGGGnnnn_n'
Cuts at: 910 991 1326 3470 910
Size: 81 335 2144 1138
Fragments arranged by size:
2144 1138 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2065 2065
Size: 3698
Bpu10I CC'TnA_GC
Cuts at: 329 527 329
Size: 198 3500
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 874 1753 2245 2543 874
Size: 879 492 298 2029
Fragments arranged by size:
2029 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 352 3402 352
Size: 3050 648
BsaAI yAC'GTr
Cuts at: 207 1051 207
Size: 844 2854
BsaBI GATnn'nnATC
Cuts at: 6 1212 3249 6
Size: 1206 2037 455
Fragments arranged by size:
2037 1206 455
BsaHI Gr'CG_yC
Cuts at: 1129 1377 2079 3575 1129
Size: 248 702 1496 1252
Fragments arranged by size:
1496 1252 702 248
BsaWI w'CCGG_w
Cuts at: 887 1408 2086 2360 2507 2918 887
Size: 521 678 274 147 411 1667
Fragments arranged by size:
1667 678 521 411 274 147
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 169 453 169
Size: 284 3414
BseYI C'CCAG_C
Cuts at: 1123 1881 2458 2954 1123
Size: 758 577 496 1867
Fragments arranged by size:
1867 758 577 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 614 614
Size: 3698
BsiEI CG_ry'CG
Cuts at: 104 1240 1286 2494 104
Size: 1136 46 1208 1308
Fragments arranged by size:
1308 1208 1136 46
BsiHKAI G_wGCw'C
Cuts at: 124 712 1490 1680 2472 124
Size: 588 778 190 792 1350
Fragments arranged by size:
1350 792 778 588 190
Bsp1286I G_dGCh'C
Cuts at: 124 523 620 712 1323 1416 1490 1680 1742
Size: 399 97 92 611 93 74 190 62
Cuts at: 1742 2472 124
Size: 730 1350
Fragments arranged by size:
1350 730 611 399 190 97 93 92
74 62
BspEI T'CCGG_A
Cuts at: 2918 2918
Size: 3698
BspHI T'CATG_A
Cuts at: 2874 2874
Size: 3698
BspMI ACCTGCnnnn'nnnn_
Cuts at: 748 962 1264 1645 748
Size: 214 302 381 2801
Fragments arranged by size:
2801 381 302 214
BsrBI CCG'CTC
Cuts at: 50 410 1990 2044 3528 3693 50
Size: 360 1580 54 1484 165 55
Fragments arranged by size:
1580 1484 360 165 55 54
BsrDI GCAATG_nn'
Cuts at: 222 1610 222
Size: 1388 2310
BsrFI r'CCGG_y
Cuts at: 1696 1877 2086 1696
Size: 181 209 3308
Fragments arranged by size:
3308 209 181
BssSI C'ACGA_G
Cuts at: 1969 2327 1969
Size: 358 3340
BstAPI GCAn_nnn'nTGC
Cuts at: 1075 1075
Size: 3698
BstBI TT'CG_AA
Cuts at: 2059 2059
Size: 3698
BstXI CCAn_nnnn'nTGG
Cuts at: 199 199
Size: 3698
BstYI r'GATC_y
Cuts at: 1 723 1190 1213 1548 1794 2806 2892 2904
Size: 722 467 23 335 246 1012 86 12
Cuts at: 2904 3250 1
Size: 346 449
Fragments arranged by size:
1012 722 467 449 346 335 246 86
23 12
BtgI C'CryG_G
Cuts at: 107 107
Size: 3698
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 308 1648 1829 1992 308
Size: 1340 181 163 2014
Fragments arranged by size:
2014 1340 181 163
BtsI GCAGTG_nn'
Cuts at: 521 941 521
Size: 420 3278
DraI TTT'AAA
Cuts at: 697 697
Size: 3698
DrdI GACnn_nn'nnGTC
Cuts at: 1404 2262 1404
Size: 858 2840
EaeI y'GGCC_r
Cuts at: 189 201 1283 1457 1848 1875 3220 189
Size: 12 1082 174 391 27 1345 667
Fragments arranged by size:
1345 1082 667 391 174 27 12
EagI C'GGCC_G
Cuts at: 1283 1283
Size: 3698
EarI CTCTTCn'nnn_
Cuts at: 473 602 1721 1931 473
Size: 129 1119 210 2240
Fragments arranged by size:
2240 1119 210 129
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 1906 2216 2362 3508 1906
Size: 310 146 1146 2096
Fragments arranged by size:
2096 1146 310 146
EcoICRI GAG'CTC
Cuts at: 122 710 122
Size: 588 3110
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1523 1955 2702 2970 1523
Size: 432 747 268 2251
Fragments arranged by size:
2251 747 432 268
EcoO109I rG'GnC_Cy
Cuts at: 345 616 617 837 345
Size: 271 1 220 3206
Fragments arranged by size:
3206 271 220 1
EcoRI G'AATT_C
Cuts at: 702 702
Size: 3698
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 1717 1717
Size: 3698
FspI TGC'GCA
Cuts at: 1479 1479
Size: 3698
HaeII r_GCGC'y
Cuts at: 170 680 1132 1380 2402 3381 170
Size: 510 452 248 1022 979 487
Fragments arranged by size:
1022 979 510 487 452 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1540 1570 1572 1602 1540
Size: 30 2 30 3636
Fragments arranged by size:
3636 30 30 2
HincII GTy'rAC
Cuts at: 737 737
Size: 3698
KasI G'GCGC_C
Cuts at: 1128 1376 1128
Size: 248 3450
KpnI G_GTAC'C
Cuts at: 718 718
Size: 3698
MluI A'CGCG_T
Cuts at: 3131 3131
Size: 3698
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 2369 2553 2369
Size: 184 3514
MscI TGG'CCA
Cuts at: 203 1459 203
Size: 1256 2442
MslI CAynn'nnrTG
Cuts at: 1687 1814 1687
Size: 127 3571
MspA1I CmG'CkG
Cuts at: 809 1123 1483 2496 2741 809
Size: 314 360 1013 245 1766
Fragments arranged by size:
1766 1013 360 314 245
NaeI GCC'GGC
Cuts at: 1879 1879
Size: 3698
NarI GG'CG_CC
Cuts at: 1129 1377 1129
Size: 248 3450
NcoI C'CATG_G
Cuts at: 107 107
Size: 3698
NgoMIV G'CCGG_C
Cuts at: 1877 1877
Size: 3698
NspI r_CATG'y
Cuts at: 751 1251 2158 751
Size: 500 907 2291
Fragments arranged by size:
2291 907 500
PciI A'CATG_T
Cuts at: 2154 2154
Size: 3698
PmeI GTTT'AAAC
Cuts at: 697 697
Size: 3698
PmlI CAC'GTG
Cuts at: 207 207
Size: 3698
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2837 2869 2837
Size: 32 3666
PpuMI rG'GwC_Cy
Cuts at: 345 345
Size: 3698
PspOMI G'GGCC_C
Cuts at: 616 616
Size: 3698
PstI C_TGCA'G
Cuts at: 511 745 1430 511
Size: 234 685 2779
Fragments arranged by size:
2779 685 234
PvuI CG_AT'CG
Cuts at: 104 104
Size: 3698
PvuII CAG'CTG
Cuts at: 1123 1483 1123
Size: 360 3338
RsrII CG'GwC_CG
Cuts at: 1893 1893
Size: 3698
SacI G_AGCT'C
Cuts at: 124 712 124
Size: 588 3110
SalI G'TCGA_C
Cuts at: 735 735
Size: 3698
SapI GCTCTTCn'nnn_
Cuts at: 473 1721 1931 473
Size: 1248 210 2240
Fragments arranged by size:
2240 1248 210
SbfI CC_TGCA'GG
Cuts at: 745 745
Size: 3698
ScaI AGT'ACT
Cuts at: 3215 3215
Size: 3698
SfcI C'TryA_G
Cuts at: 33 507 626 741 1426 2419 2610 33
Size: 474 119 115 685 993 191 1121
Fragments arranged by size:
1121 993 685 474 191 119 115
SfoI GGC'GCC
Cuts at: 1130 1378 1130
Size: 248 3450
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 3698
SmaI CCC'GGG
Cuts at: 136 720 136
Size: 584 3114
SmlI C'TyrA_G
Cuts at: 853 1768 2260 2522 853
Size: 915 492 262 2029
Fragments arranged by size:
2029 915 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 3698
SphI G_CATG'C
Cuts at: 751 1251 751
Size: 500 3198
StyI C'CwwG_G
Cuts at: 107 125 233 335 353 575 832 1186 107
Size: 18 108 102 18 222 257 354 2619
Fragments arranged by size:
2619 354 257 222 108 102 18 18
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 483 1281 1414 1873 2082 483
Size: 798 133 459 209 2099
Fragments arranged by size:
2099 798 459 209 133
TatI w'GTAC_w
Cuts at: 3213 3213
Size: 3698
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 426 458 3215 3247 426
Size: 32 2757 32 877
Fragments arranged by size:
2757 877 32 32
Tth111I GACn'n_nGTC
Cuts at: 1495 1495
Size: 3698
XbaI T'CTAG_A
Cuts at: 729 729
Size: 3698
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1193 1193
Size: 3698
XmaI C'CCGG_G
Cuts at: 134 718 134
Size: 584 3114
XmnI GAAnn'nnTTC
Cuts at: 379 397 379
Size: 18 3680
Enzymes that do cut:
AccI Acc65I AclI AcuI AfeI AflIII AgeI AlwNI
ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI
BanII BbeI BbvCI BcgI BciVI BclI BglI BglII
BlpI Bme1580I BmgBI BmrI BplI Bpu10I BpuEI BsaI
BsaAI BsaBI BsaHI BsaWI BseRI BseYI BsgI BsiEI
BsiHKAI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BssSI BstAPI BstBI BstXI BstYI BtgI BtgZI BtsI
DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI
EcoO109I EcoRI FalI FspI HaeII Hin4I HincII KasI
KpnI MluI MmeI MscI MslI MspA1I NaeI NarI
NcoI NgoMIV NspI PciI PmeI PmlI PpiI PpuMI
PspOMI PstI PvuI PvuII RsrII SacI SalI SapI
SbfI ScaI SfcI SfoI SgfI SmaI SmlI SpeI
SphI StyI TaqII TatI TstI Tth111I XbaI XcmI
XmaI XmnI
Enzymes that do not cut:
AarI AatII AflII AhdI AleI AlfI AloI AscI
AvrII BaeI BbsI BfrBI BmtI BpmI BsaXI BsiWI
BsmI BsmBI BsrGI BssHII BstEII BstZ17I Bsu36I ClaI
CspCI DraIII EcoNI EcoRV FseI FspAI HindIII HpaI
MfeI NdeI NheI NotI NruI NsiI PacI PasI
PflMI PfoI PshAI PsiI PspXI PsrI SacII SanDI
SexAI SfiI SgrAI SnaBI SrfI SspI StuI SwaI
XhoI ZraI