Restriction map for
pF1KE0110
(Circular) (Six-base) MAPSORT of: pF1KE0110.seq Check: 2015 from: 1 to: 3890
pF1KE0110 3890 bp
With 182 enzymes: SgfI *
April 24, 2009 13:10 ..
AccI GT'mk_AC
Cuts at: 928 928
Size: 3890
Acc65I G'GTAC_C
Cuts at: 906 906
Size: 3890
AclI AA'CG_TT
Cuts at: 3730 3730
Size: 3890
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 332 1715 2147 2894 3162 332
Size: 1383 432 747 268 1060
Fragments arranged by size:
1383 1060 747 432 268
AflIII A'CryG_T
Cuts at: 1242 2346 3323 1242
Size: 1104 977 1809
Fragments arranged by size:
1809 1104 977
AgeI A'CCGG_T
Cuts at: 2278 2278
Size: 3890
AleI CACnn'nnGTG
Cuts at: 634 634
Size: 3890
AlwNI CAG_nnn'CTG
Cuts at: 1195 2762 1195
Size: 1567 2323
ApaLI G'TGCA_C
Cuts at: 2660 2660
Size: 3890
ApoI r'AATT_y
Cuts at: 298 482 894 3243 3499 298
Size: 184 412 2349 256 689
Fragments arranged by size:
2349 689 412 256 184
AseI AT'TA_AT
Cuts at: 20 815 20
Size: 795 3095
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 3890
AvaI C'yCGr_G
Cuts at: 910 910
Size: 3890
BamHI G'GATC_C
Cuts at: 915 915
Size: 3890
BanI G'GyrC_C
Cuts at: 459 906 1320 1568 1603 459
Size: 447 414 248 35 2746
Fragments arranged by size:
2746 447 414 248 35
BanII G_rGCy'C
Cuts at: 747 904 1934 747
Size: 157 1030 2703
Fragments arranged by size:
2703 1030 157
BbeI G_GCGC'C
Cuts at: 1324 1572 1324
Size: 248 3642
BbsI GAAGACnn'nnnn_
Cuts at: 658 658
Size: 3890
BciVI GTATCCnnnnn_n'
Cuts at: 1788 2549 3890 1788
Size: 761 1341 1788
Fragments arranged by size:
1788 1341 761
BclI T'GATC_A
Cuts at: 1410 1410
Size: 3890
BglII A'GATC_T
Cuts at: 144 1405 3442 144
Size: 1261 2037 592
Fragments arranged by size:
2037 1261 592
BlpI GC'TnA_GC
Cuts at: 570 1002 570
Size: 432 3458
Bme1580I G_kGCm'C
Cuts at: 402 682 1515 1608 2664 402
Size: 280 833 93 1056 1628
Fragments arranged by size:
1628 1056 833 280 93
BmrI ACTGGGnnnn_n'
Cuts at: 791 1102 1183 1518 3662 791
Size: 311 81 335 2144 1019
Fragments arranged by size:
2144 1019 335 311 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 866 2257 866
Size: 1391 2499
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 731 1066 1945 2437 2735 731
Size: 335 879 492 298 1886
Fragments arranged by size:
1886 879 492 335 298
BsaI GGTCTCn'nnnn_
Cuts at: 3594 3594
Size: 3890
BsaAI yAC'GTr
Cuts at: 214 1243 214
Size: 1029 2861
BsaBI GATnn'nnATC
Cuts at: 6 532 1404 3441 6
Size: 526 872 2037 455
Fragments arranged by size:
2037 872 526 455
BsaHI Gr'CG_yC
Cuts at: 1321 1569 2271 3767 1321
Size: 248 702 1496 1444
Fragments arranged by size:
1496 1444 702 248
BsaWI w'CCGG_w
Cuts at: 500 1079 1600 2278 2552 2699 3110 500
Size: 579 521 678 274 147 411 1280
Fragments arranged by size:
1280 678 579 521 411 274 147
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 712 712
Size: 3890
BseYI C'CCAG_C
Cuts at: 762 1315 2073 2650 3146 762
Size: 553 758 577 496 1506
Fragments arranged by size:
1506 758 577 553 496
BsiEI CG_ry'CG
Cuts at: 104 1432 1478 2686 104
Size: 1328 46 1208 1308
Fragments arranged by size:
1328 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 522 904 1682 1872 2664 522
Size: 382 778 190 792 1748
Fragments arranged by size:
1748 792 778 382 190
BsmI GAATG_Cn'
Cuts at: 496 496
Size: 3890
Bsp1286I G_dGCh'C
Cuts at: 402 522 682 747 904 1515 1608 1682 1872
Size: 120 160 65 157 611 93 74 190
Cuts at: 1872 1934 2664 402
Size: 62 730 1628
Fragments arranged by size:
1628 730 611 190 160 157 120 93
74 65 62
BspEI T'CCGG_A
Cuts at: 3110 3110
Size: 3890
BspHI T'CATG_A
Cuts at: 707 3066 707
Size: 2359 1531
BspMI ACCTGCnnnn'nnnn_
Cuts at: 940 1154 1456 1837 940
Size: 214 302 381 2993
Fragments arranged by size:
2993 381 302 214
BsrBI CCG'CTC
Cuts at: 50 360 2182 2236 3720 3885 50
Size: 310 1822 54 1484 165 55
Fragments arranged by size:
1822 1484 310 165 55 54
BsrDI GCAATG_nn'
Cuts at: 670 1802 670
Size: 1132 2758
BsrFI r'CCGG_y
Cuts at: 1888 2069 2278 1888
Size: 181 209 3500
Fragments arranged by size:
3500 209 181
BssSI C'ACGA_G
Cuts at: 2161 2519 2161
Size: 358 3532
BstAPI GCAn_nnn'nTGC
Cuts at: 121 1267 121
Size: 1146 2744
BstBI TT'CG_AA
Cuts at: 2251 2251
Size: 3890
BstXI CCAn_nnnn'nTGG
Cuts at: 636 636
Size: 3890
BstYI r'GATC_y
Cuts at: 1 144 915 1382 1405 1740 1986 2998 3084
Size: 143 771 467 23 335 246 1012 86
Cuts at: 3084 3096 3442 1
Size: 12 346 449
Fragments arranged by size:
1012 771 467 449 346 335 246 143
86 23 12
Bsu36I CC'TnA_GG
Cuts at: 695 695
Size: 3890
BtgI C'CryG_G
Cuts at: 107 107
Size: 3890
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 1840 2021 2184 1840
Size: 181 163 3546
Fragments arranged by size:
3546 181 163
BtsI GCAGTG_nn'
Cuts at: 1133 1133
Size: 3890
DraI TTT'AAA
Cuts at: 889 889
Size: 3890
DrdI GACnn_nn'nnGTC
Cuts at: 1596 2454 1596
Size: 858 3032
EaeI y'GGCC_r
Cuts at: 1475 1649 2040 2067 3412 1475
Size: 174 391 27 1345 1953
Fragments arranged by size:
1953 1345 391 174 27
EagI C'GGCC_G
Cuts at: 1475 1475
Size: 3890
EarI CTCTTCn'nnn_
Cuts at: 1913 2123 1913
Size: 210 3680
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2098 2408 2554 3700 2098
Size: 310 146 1146 2288
Fragments arranged by size:
2288 1146 310 146
EcoICRI GAG'CTC
Cuts at: 902 902
Size: 3890
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 332 1715 2147 2894 3162 332
Size: 1383 432 747 268 1060
Fragments arranged by size:
1383 1060 747 432 268
EcoO109I rG'GnC_Cy
Cuts at: 580 1029 580
Size: 449 3441
EcoRI G'AATT_C
Cuts at: 894 894
Size: 3890
EcoRV GAT'ATC
Cuts at: 306 306
Size: 3890
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 675 1909 675
Size: 1234 2656
FspI TGC'GCA
Cuts at: 1671 1671
Size: 3890
HaeII r_GCGC'y
Cuts at: 1324 1572 2594 3573 1324
Size: 248 1022 979 1641
Fragments arranged by size:
1641 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1732 1762 1764 1794 1732
Size: 30 2 30 3828
Fragments arranged by size:
3828 30 30 2
HincII GTy'rAC
Cuts at: 929 929
Size: 3890
KasI G'GCGC_C
Cuts at: 1320 1568 1320
Size: 248 3642
KpnI G_GTAC'C
Cuts at: 910 910
Size: 3890
MluI A'CGCG_T
Cuts at: 3323 3323
Size: 3890
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 2561 2745 2561
Size: 184 3706
MscI TGG'CCA
Cuts at: 1651 1651
Size: 3890
MslI CAynn'nnrTG
Cuts at: 634 1879 2006 634
Size: 1245 127 2518
Fragments arranged by size:
2518 1245 127
MspA1I CmG'CkG
Cuts at: 178 1001 1315 1675 2688 2933 178
Size: 823 314 360 1013 245 1135
Fragments arranged by size:
1135 1013 823 360 314 245
NaeI GCC'GGC
Cuts at: 2071 2071
Size: 3890
NarI GG'CG_CC
Cuts at: 1321 1569 1321
Size: 248 3642
NcoI C'CATG_G
Cuts at: 107 107
Size: 3890
NgoMIV G'CCGG_C
Cuts at: 2069 2069
Size: 3890
NspI r_CATG'y
Cuts at: 119 762 943 1443 2350 119
Size: 643 181 500 907 1659
Fragments arranged by size:
1659 907 643 500 181
PasI CC'CwG_GG
Cuts at: 576 576
Size: 3890
PciI A'CATG_T
Cuts at: 2346 2346
Size: 3890
PfoI T'CCnGG_A
Cuts at: 509 509
Size: 3890
PmeI GTTT'AAAC
Cuts at: 889 889
Size: 3890
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 640 672 3029 3061 640
Size: 32 2357 32 1469
Fragments arranged by size:
2357 1469 32 32
PstI C_TGCA'G
Cuts at: 342 875 937 1622 342
Size: 533 62 685 2610
Fragments arranged by size:
2610 685 533 62
PvuI CG_AT'CG
Cuts at: 104 104
Size: 3890
PvuII CAG'CTG
Cuts at: 178 1315 1675 178
Size: 1137 360 2393
Fragments arranged by size:
2393 1137 360
RsrII CG'GwC_CG
Cuts at: 2085 2085
Size: 3890
SacI G_AGCT'C
Cuts at: 904 904
Size: 3890
SalI G'TCGA_C
Cuts at: 927 927
Size: 3890
SapI GCTCTTCn'nnn_
Cuts at: 1913 2123 1913
Size: 210 3680
SbfI CC_TGCA'GG
Cuts at: 937 937
Size: 3890
ScaI AGT'ACT
Cuts at: 3407 3407
Size: 3890
SfcI C'TryA_G
Cuts at: 33 338 871 933 1618 2611 2802 33
Size: 305 533 62 685 993 191 1121
Fragments arranged by size:
1121 993 685 533 305 191 62
SfoI GGC'GCC
Cuts at: 1322 1570 1322
Size: 248 3642
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 3890
SmaI CCC'GGG
Cuts at: 912 912
Size: 3890
SmlI C'TyrA_G
Cuts at: 746 1045 1960 2452 2714 746
Size: 299 915 492 262 1922
Fragments arranged by size:
1922 915 492 299 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 3890
SphI G_CATG'C
Cuts at: 119 762 943 1443 119
Size: 643 181 500 2566
Fragments arranged by size:
2566 643 500 181
SspI AAT'ATT
Cuts at: 323 323
Size: 3890
StyI C'CwwG_G
Cuts at: 107 1024 1378 107
Size: 917 354 2619
Fragments arranged by size:
2619 917 354
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1473 1606 2065 2274 1473
Size: 133 459 209 3089
Fragments arranged by size:
3089 459 209 133
TatI w'GTAC_w
Cuts at: 3405 3405
Size: 3890
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 578 610 3407 3439 578
Size: 32 2797 32 1029
Fragments arranged by size:
2797 1029 32 32
Tth111I GACn'n_nGTC
Cuts at: 1687 1687
Size: 3890
XbaI T'CTAG_A
Cuts at: 921 921
Size: 3890
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1385 1385
Size: 3890
XmaI C'CCGG_G
Cuts at: 910 910
Size: 3890
XmnI GAAnn'nnTTC
Cuts at: 470 655 470
Size: 185 3705
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AgeI AleI AlwNI
ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII
BbeI BbsI BciVI BclI BglII BlpI Bme1580I BmrI
BplI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BseRI
BseYI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI
BsrBI BsrDI BsrFI BssSI BstAPI BstBI BstXI BstYI
Bsu36I BtgI BtgZI BtsI DraI DrdI EaeI EagI
EarI EciI EcoICRI Eco57MI EcoO109I EcoRI EcoRV FalI
FspI HaeII Hin4I HincII KasI KpnI MluI MmeI
MscI MslI MspA1I NaeI NarI NcoI NgoMIV NspI
PasI PciI PfoI PmeI PpiI PstI PvuI PvuII
RsrII SacI SalI SapI SbfI ScaI SfcI SfoI
SgfI SmaI SmlI SpeI SphI SspI StyI TaqII
TatI TstI Tth111I XbaI XcmI XmaI XmnI
Enzymes that do not cut:
AarI AatII AfeI AflII AhdI AlfI AloI ApaI
AscI AvrII BaeI BbvCI BcgI BfrBI BglI BmgBI
BmtI BpmI Bpu10I BsaXI BsgI BsiWI BsmBI BsrGI
BssHII BstEII BstZ17I ClaI CspCI DraIII EcoNI FseI
FspAI HindIII HpaI MfeI NdeI NheI NotI NruI
NsiI PacI PflMI PmlI PpuMI PshAI PsiI PspOMI
PspXI PsrI SacII SanDI SexAI SfiI SgrAI SnaBI
SrfI StuI SwaI XhoI ZraI