Restriction map for
pF1KE0105
(Circular) (Six-base) MAPSORT of: pF1KE0105.seq Check: 4193 from: 1 to: 3656
pF1KE0105 3656 bp
With 182 enzymes: SgfI *
April 24, 2009 13:10 ..
AccI GT'mk_AC
Cuts at: 694 694
Size: 3656
Acc65I G'GTAC_C
Cuts at: 672 672
Size: 3656
AclI AA'CG_TT
Cuts at: 3496 3496
Size: 3656
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1481 1913 2660 2928 1481
Size: 432 747 268 2209
Fragments arranged by size:
2209 747 432 268
AflIII A'CryG_T
Cuts at: 1008 2112 3089 1008
Size: 1104 977 1575
Fragments arranged by size:
1575 1104 977
AgeI A'CCGG_T
Cuts at: 2044 2044
Size: 3656
AlwNI CAG_nnn'CTG
Cuts at: 961 2528 961
Size: 1567 2089
ApaLI G'TGCA_C
Cuts at: 2426 2426
Size: 3656
ApoI r'AATT_y
Cuts at: 539 660 3009 3265 539
Size: 121 2349 256 930
Fragments arranged by size:
2349 930 256 121
AseI AT'TA_AT
Cuts at: 20 20
Size: 3656
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 3656
AvaI C'yCGr_G
Cuts at: 676 676
Size: 3656
BamHI G'GATC_C
Cuts at: 681 681
Size: 3656
BanI G'GyrC_C
Cuts at: 604 672 1086 1334 1369 604
Size: 68 414 248 35 2891
Fragments arranged by size:
2891 414 248 68 35
BanII G_rGCy'C
Cuts at: 229 670 1700 229
Size: 441 1030 2185
Fragments arranged by size:
2185 1030 441
BbeI G_GCGC'C
Cuts at: 1090 1338 1090
Size: 248 3408
BbsI GAAGACnn'nnnn_
Cuts at: 647 647
Size: 3656
BciVI GTATCCnnnnn_n'
Cuts at: 1554 2315 3656 1554
Size: 761 1341 1554
Fragments arranged by size:
1554 1341 761
BclI T'GATC_A
Cuts at: 1176 1176
Size: 3656
BglII A'GATC_T
Cuts at: 1171 3208 1171
Size: 2037 1619
BlpI GC'TnA_GC
Cuts at: 223 325 768 223
Size: 102 443 3111
Fragments arranged by size:
3111 443 102
Bme1580I G_kGCm'C
Cuts at: 1281 1374 2430 1281
Size: 93 1056 2507
Fragments arranged by size:
2507 1056 93
BmrI ACTGGGnnnn_n'
Cuts at: 567 868 949 1284 3428 567
Size: 301 81 335 2144 795
Fragments arranged by size:
2144 795 335 301 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2023 2023
Size: 3656
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 832 1711 2203 2501 832
Size: 879 492 298 1987
Fragments arranged by size:
1987 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 3360 3360
Size: 3656
BsaAI yAC'GTr
Cuts at: 1009 1009
Size: 3656
BsaBI GATnn'nnATC
Cuts at: 6 412 1170 3207 6
Size: 406 758 2037 455
Fragments arranged by size:
2037 758 455 406
BsaHI Gr'CG_yC
Cuts at: 1087 1335 2037 3533 1087
Size: 248 702 1496 1210
Fragments arranged by size:
1496 1210 702 248
BsaWI w'CCGG_w
Cuts at: 845 1366 2044 2318 2465 2876 845
Size: 521 678 274 147 411 1625
Fragments arranged by size:
1625 678 521 411 274 147
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 148 148
Size: 3656
BseYI C'CCAG_C
Cuts at: 1081 1839 2416 2912 1081
Size: 758 577 496 1825
Fragments arranged by size:
1825 758 577 496
BsiEI CG_ry'CG
Cuts at: 104 1198 1244 2452 104
Size: 1094 46 1208 1308
Fragments arranged by size:
1308 1208 1094 46
BsiHKAI G_wGCw'C
Cuts at: 670 1448 1638 2430 670
Size: 778 190 792 1896
Fragments arranged by size:
1896 792 778 190
BsmI GAATG_Cn'
Cuts at: 391 391
Size: 3656
Bsp1286I G_dGCh'C
Cuts at: 229 670 1281 1374 1448 1638 1700 2430 229
Size: 441 611 93 74 190 62 730 1455
Fragments arranged by size:
1455 730 611 441 190 93 74 62
BspEI T'CCGG_A
Cuts at: 2876 2876
Size: 3656
BspHI T'CATG_A
Cuts at: 2832 2832
Size: 3656
BspMI ACCTGCnnnn'nnnn_
Cuts at: 706 920 1222 1603 706
Size: 214 302 381 2759
Fragments arranged by size:
2759 381 302 214
BsrBI CCG'CTC
Cuts at: 50 1948 2002 3486 3651 50
Size: 1898 54 1484 165 55
Fragments arranged by size:
1898 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 386 1568 386
Size: 1182 2474
BsrFI r'CCGG_y
Cuts at: 1654 1835 2044 1654
Size: 181 209 3266
Fragments arranged by size:
3266 209 181
BssSI C'ACGA_G
Cuts at: 1927 2285 1927
Size: 358 3298
BstAPI GCAn_nnn'nTGC
Cuts at: 1033 1033
Size: 3656
BstBI TT'CG_AA
Cuts at: 2017 2017
Size: 3656
BstYI r'GATC_y
Cuts at: 1 424 681 1148 1171 1506 1752 2764 2850
Size: 423 257 467 23 335 246 1012 86
Cuts at: 2850 2862 3208 1
Size: 12 346 449
Fragments arranged by size:
1012 467 449 423 346 335 257 246
86 23 12
BtgI C'CryG_G
Cuts at: 107 107
Size: 3656
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 1606 1787 1950 1606
Size: 181 163 3312
Fragments arranged by size:
3312 181 163
BtsI GCAGTG_nn'
Cuts at: 309 899 309
Size: 590 3066
DraI TTT'AAA
Cuts at: 655 655
Size: 3656
DrdI GACnn_nn'nnGTC
Cuts at: 1362 2220 1362
Size: 858 2798
EaeI y'GGCC_r
Cuts at: 1241 1415 1806 1833 3178 1241
Size: 174 391 27 1345 1719
Fragments arranged by size:
1719 1345 391 174 27
EagI C'GGCC_G
Cuts at: 1241 1241
Size: 3656
EarI CTCTTCn'nnn_
Cuts at: 1679 1889 1679
Size: 210 3446
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 1864 2174 2320 3466 1864
Size: 310 146 1146 2054
Fragments arranged by size:
2054 1146 310 146
EcoICRI GAG'CTC
Cuts at: 668 668
Size: 3656
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1481 1913 2660 2928 1481
Size: 432 747 268 2209
Fragments arranged by size:
2209 747 432 268
EcoO109I rG'GnC_Cy
Cuts at: 795 795
Size: 3656
EcoRI G'AATT_C
Cuts at: 660 660
Size: 3656
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 1675 1675
Size: 3656
FspI TGC'GCA
Cuts at: 1437 1437
Size: 3656
HaeII r_GCGC'y
Cuts at: 199 374 1090 1338 2360 3339 199
Size: 175 716 248 1022 979 516
Fragments arranged by size:
1022 979 716 516 248 175
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 333 365 1498 1528 1530 1560 333
Size: 32 1133 30 2 30 2429
Fragments arranged by size:
2429 1133 32 30 30 2
HincII GTy'rAC
Cuts at: 695 695
Size: 3656
KasI G'GCGC_C
Cuts at: 1086 1334 1086
Size: 248 3408
KpnI G_GTAC'C
Cuts at: 676 676
Size: 3656
MluI A'CGCG_T
Cuts at: 3089 3089
Size: 3656
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 2327 2511 2327
Size: 184 3472
MscI TGG'CCA
Cuts at: 1417 1417
Size: 3656
MslI CAynn'nnrTG
Cuts at: 1645 1772 1645
Size: 127 3529
MspA1I CmG'CkG
Cuts at: 125 244 767 1081 1441 2454 2699 125
Size: 119 523 314 360 1013 245 1082
Fragments arranged by size:
1082 1013 523 360 314 245 119
NaeI GCC'GGC
Cuts at: 1837 1837
Size: 3656
NarI GG'CG_CC
Cuts at: 1087 1335 1087
Size: 248 3408
NcoI C'CATG_G
Cuts at: 107 107
Size: 3656
NgoMIV G'CCGG_C
Cuts at: 1835 1835
Size: 3656
NspI r_CATG'y
Cuts at: 709 1209 2116 709
Size: 500 907 2249
Fragments arranged by size:
2249 907 500
PciI A'CATG_T
Cuts at: 2112 2112
Size: 3656
PmeI GTTT'AAAC
Cuts at: 655 655
Size: 3656
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2795 2827 2795
Size: 32 3624
PstI C_TGCA'G
Cuts at: 703 1388 703
Size: 685 2971
PvuI CG_AT'CG
Cuts at: 104 104
Size: 3656
PvuII CAG'CTG
Cuts at: 244 1081 1441 244
Size: 837 360 2459
Fragments arranged by size:
2459 837 360
RsrII CG'GwC_CG
Cuts at: 1851 1851
Size: 3656
SacI G_AGCT'C
Cuts at: 670 670
Size: 3656
SalI G'TCGA_C
Cuts at: 693 693
Size: 3656
SapI GCTCTTCn'nnn_
Cuts at: 1679 1889 1679
Size: 210 3446
SbfI CC_TGCA'GG
Cuts at: 703 703
Size: 3656
ScaI AGT'ACT
Cuts at: 256 3173 256
Size: 2917 739
SfcI C'TryA_G
Cuts at: 33 441 699 1384 2377 2568 33
Size: 408 258 685 993 191 1121
Fragments arranged by size:
1121 993 685 408 258 191
SfoI GGC'GCC
Cuts at: 1088 1336 1088
Size: 248 3408
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 3656
SmaI CCC'GGG
Cuts at: 678 678
Size: 3656
SmlI C'TyrA_G
Cuts at: 811 1726 2218 2480 811
Size: 915 492 262 1987
Fragments arranged by size:
1987 915 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 3656
SphI G_CATG'C
Cuts at: 709 1209 709
Size: 500 3156
StyI C'CwwG_G
Cuts at: 107 790 1144 107
Size: 683 354 2619
Fragments arranged by size:
2619 683 354
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1239 1372 1831 2040 1239
Size: 133 459 209 2855
Fragments arranged by size:
2855 459 209 133
TatI w'GTAC_w
Cuts at: 254 3171 254
Size: 2917 739
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 301 333 3173 3205 301
Size: 32 2840 32 752
Fragments arranged by size:
2840 752 32 32
Tth111I GACn'n_nGTC
Cuts at: 1453 1453
Size: 3656
XbaI T'CTAG_A
Cuts at: 687 687
Size: 3656
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1151 1151
Size: 3656
XmaI C'CCGG_G
Cuts at: 676 676
Size: 3656
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AgeI AlwNI ApaLI
ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI
BbsI BciVI BclI BglII BlpI Bme1580I BmrI BplI
BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BseRI BseYI
BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI
BsrDI BsrFI BssSI BstAPI BstBI BstYI BtgI BtgZI
BtsI DraI DrdI EaeI EagI EarI EciI EcoICRI
Eco57MI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII
KasI KpnI MluI MmeI MscI MslI MspA1I NaeI
NarI NcoI NgoMIV NspI PciI PmeI PpiI PstI
PvuI PvuII RsrII SacI SalI SapI SbfI ScaI
SfcI SfoI SgfI SmaI SmlI SpeI SphI StyI
TaqII TatI TstI Tth111I XbaI XcmI XmaI
Enzymes that do not cut:
AarI AatII AfeI AflII AhdI AleI AlfI AloI
ApaI AscI AvrII BaeI BbvCI BcgI BfrBI BglI
BmgBI BmtI BpmI Bpu10I BsaXI BsgI BsiWI BsmBI
BsrGI BssHII BstEII BstXI BstZ17I Bsu36I ClaI CspCI
DraIII EcoNI EcoRV FseI FspAI HindIII HpaI MfeI
NdeI NheI NotI NruI NsiI PacI PasI PflMI
PfoI PmlI PpuMI PshAI PsiI PspOMI PspXI PsrI
SacII SanDI SexAI SfiI SgrAI SnaBI SrfI SspI
StuI SwaI XhoI XmnI ZraI