Restriction map for
pF1KE0103
(Circular) (Six-base) MAPSORT of: pF1KE0103.seq Check: 8109 from: 1 to: 4313
pF1KE0103 4313 bp
With 182 enzymes: SgfI *
April 24, 2009 13:10 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 510 510
Size: 4313
AccI GT'mk_AC
Cuts at: 707 1351 707
Size: 644 3669
Acc65I G'GTAC_C
Cuts at: 1329 1329
Size: 4313
AclI AA'CG_TT
Cuts at: 4153 4153
Size: 4313
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 2138 2570 3317 3585 2138
Size: 432 747 268 2866
Fragments arranged by size:
2866 747 432 268
AflIII A'CryG_T
Cuts at: 147 1665 2769 3746 147
Size: 1518 1104 977 714
Fragments arranged by size:
1518 1104 977 714
AgeI A'CCGG_T
Cuts at: 2701 2701
Size: 4313
AleI CACnn'nnGTG
Cuts at: 1105 1105
Size: 4313
AlwNI CAG_nnn'CTG
Cuts at: 1618 3185 1618
Size: 1567 2746
ApaI G_GGCC'C
Cuts at: 877 1236 877
Size: 359 3954
ApaLI G'TGCA_C
Cuts at: 373 3083 373
Size: 2710 1603
ApoI r'AATT_y
Cuts at: 1317 3666 3922 1317
Size: 2349 256 1708
Fragments arranged by size:
2349 1708 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 4313
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4313
AvaI C'yCGr_G
Cuts at: 204 1333 204
Size: 1129 3184
BamHI G'GATC_C
Cuts at: 1091 1338 1091
Size: 247 4066
BanI G'GyrC_C
Cuts at: 385 1287 1329 1743 1991 2026 385
Size: 902 42 414 248 35 2672
Fragments arranged by size:
2672 902 414 248 42 35
BanII G_rGCy'C
Cuts at: 227 311 877 1236 1327 2357 227
Size: 84 566 359 91 1030 2183
Fragments arranged by size:
2183 1030 566 359 91 84
BbeI G_GCGC'C
Cuts at: 389 1747 1995 389
Size: 1358 248 2707
Fragments arranged by size:
2707 1358 248
BbsI GAAGACnn'nnnn_
Cuts at: 289 1033 289
Size: 744 3569
BbvCI CC'TCA_GC
Cuts at: 302 302
Size: 4313
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 570 604 570
Size: 34 4279
BciVI GTATCCnnnnn_n'
Cuts at: 815 942 2211 2972 4313 815
Size: 127 1269 761 1341 815
Fragments arranged by size:
1341 1269 815 761 127
BclI T'GATC_A
Cuts at: 1833 1833
Size: 4313
BglI GCCn_nnn'nGGC
Cuts at: 1252 1252
Size: 4313
BglII A'GATC_T
Cuts at: 1828 3865 1828
Size: 2037 2276
BlpI GC'TnA_GC
Cuts at: 193 1425 193
Size: 1232 3081
Bme1580I G_kGCm'C
Cuts at: 377 877 1236 1265 1938 2031 3087 377
Size: 500 359 29 673 93 1056 1603
Fragments arranged by size:
1603 1056 673 500 359 93 29
BmrI ACTGGGnnnn_n'
Cuts at: 611 1525 1606 1941 4085 611
Size: 914 81 335 2144 839
Fragments arranged by size:
2144 914 839 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 327 2680 327
Size: 2353 1960
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 760 760
Size: 4313
Bpu10I CC'TnA_GC
Cuts at: 302 724 302
Size: 422 3891
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1489 2368 2860 3158 1489
Size: 879 492 298 2644
Fragments arranged by size:
2644 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 193 1124 4017 193
Size: 931 2893 489
Fragments arranged by size:
2893 931 489
BsaAI yAC'GTr
Cuts at: 462 885 1666 462
Size: 423 781 3109
Fragments arranged by size:
3109 781 423
BsaBI GATnn'nnATC
Cuts at: 6 1827 3864 6
Size: 1821 2037 455
Fragments arranged by size:
2037 1821 455
BsaHI Gr'CG_yC
Cuts at: 386 1744 1992 2694 4190 386
Size: 1358 248 702 1496 509
Fragments arranged by size:
1496 1358 702 509 248
BsaWI w'CCGG_w
Cuts at: 351 1502 2023 2701 2975 3122 3533 351
Size: 1151 521 678 274 147 411 1131
Fragments arranged by size:
1151 1131 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 523 553 523
Size: 30 4283
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 505 1003 1228 505
Size: 498 225 3590
Fragments arranged by size:
3590 498 225
BseYI C'CCAG_C
Cuts at: 170 876 1738 2496 3073 3569 170
Size: 706 862 758 577 496 914
Fragments arranged by size:
914 862 758 706 577 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 949 949
Size: 4313
BsiEI CG_ry'CG
Cuts at: 104 594 1855 1901 3109 104
Size: 490 1261 46 1208 1308
Fragments arranged by size:
1308 1261 1208 490 46
BsiHKAI G_wGCw'C
Cuts at: 377 1327 2105 2295 3087 377
Size: 950 778 190 792 1603
Fragments arranged by size:
1603 950 792 778 190
BsmBI CGTCTCn'nnnn_
Cuts at: 526 526
Size: 4313
Bsp1286I G_dGCh'C
Cuts at: 227 311 377 877 1236 1265 1327 1938 2031
Size: 84 66 500 359 29 62 611 93
Cuts at: 2031 2105 2295 2357 3087 227
Size: 74 190 62 730 1453
Fragments arranged by size:
1453 730 611 500 359 190 93 84
74 66 62 62 29
BspEI T'CCGG_A
Cuts at: 351 3533 351
Size: 3182 1131
BspHI T'CATG_A
Cuts at: 3489 3489
Size: 4313
BspMI ACCTGCnnnn'nnnn_
Cuts at: 510 662 1363 1577 1879 2260 510
Size: 152 701 214 302 381 2563
Fragments arranged by size:
2563 701 381 302 214 152
BsrBI CCG'CTC
Cuts at: 50 854 2605 2659 4143 4308 50
Size: 804 1751 54 1484 165 55
Fragments arranged by size:
1751 1484 804 165 55 54
BsrDI GCAATG_nn'
Cuts at: 1241 2225 1241
Size: 984 3329
BsrFI r'CCGG_y
Cuts at: 1246 2311 2492 2701 1246
Size: 1065 181 209 2858
Fragments arranged by size:
2858 1065 209 181
BssSI C'ACGA_G
Cuts at: 2584 2942 2584
Size: 358 3955
BstAPI GCAn_nnn'nTGC
Cuts at: 1690 1690
Size: 4313
BstBI TT'CG_AA
Cuts at: 2674 2674
Size: 4313
BstEII G'GTnAC_C
Cuts at: 913 913
Size: 4313
BstXI CCAn_nnnn'nTGG
Cuts at: 624 630 1068 1302 624
Size: 6 438 234 3635
Fragments arranged by size:
3635 438 234 6
BstYI r'GATC_y
Cuts at: 1 1091 1338 1805 1828 2163 2409 3421 3507
Size: 1090 247 467 23 335 246 1012 86
Cuts at: 3507 3519 3865 1
Size: 12 346 449
Fragments arranged by size:
1090 1012 467 449 346 335 247 246
86 23 12
BtgI C'CryG_G
Cuts at: 107 623 768 969 1196 107
Size: 516 145 201 227 3224
Fragments arranged by size:
3224 516 227 201 145
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 872 2263 2444 2607 872
Size: 1391 181 163 2578
Fragments arranged by size:
2578 1391 181 163
BtsI GCAGTG_nn'
Cuts at: 635 1556 635
Size: 921 3392
DraI TTT'AAA
Cuts at: 1312 1312
Size: 4313
DraIII CAC_nnn'GTG
Cuts at: 345 345
Size: 4313
DrdI GACnn_nn'nnGTC
Cuts at: 826 922 2019 2877 826
Size: 96 1097 858 2262
Fragments arranged by size:
2262 1097 858 96
EaeI y'GGCC_r
Cuts at: 110 603 1058 1172 1898 2072 2463 2490 3835
Size: 493 455 114 726 174 391 27 1345
Cuts at: 3835 110
Size: 588
Fragments arranged by size:
1345 726 588 493 455 391 174 114
27
EagI C'GGCC_G
Cuts at: 1898 1898
Size: 4313
EarI CTCTTCn'nnn_
Cuts at: 1219 2336 2546 1219
Size: 1117 210 2986
Fragments arranged by size:
2986 1117 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2521 2831 2977 4123 2521
Size: 310 146 1146 2711
Fragments arranged by size:
2711 1146 310 146
EcoICRI GAG'CTC
Cuts at: 1325 1325
Size: 4313
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 760 2138 2570 3317 3585 760
Size: 1378 432 747 268 1488
Fragments arranged by size:
1488 1378 747 432 268
EcoO109I rG'GnC_Cy
Cuts at: 744 1035 1233 1452 744
Size: 291 198 219 3605
Fragments arranged by size:
3605 291 219 198
EcoRI G'AATT_C
Cuts at: 1317 1317
Size: 4313
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2332 2332
Size: 4313
FspI TGC'GCA
Cuts at: 2094 2094
Size: 4313
HaeII r_GCGC'y
Cuts at: 389 1747 1995 3017 3996 389
Size: 1358 248 1022 979 706
Fragments arranged by size:
1358 1022 979 706 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 134 166 1121 1153 2155 2185 2187 2217 134
Size: 32 955 32 1002 30 2 30 2230
Fragments arranged by size:
2230 1002 955 32 32 30 30 2
HincII GTy'rAC
Cuts at: 708 1140 1352 708
Size: 432 212 3669
Fragments arranged by size:
3669 432 212
HindIII A'AGCT_T
Cuts at: 403 403
Size: 4313
KasI G'GCGC_C
Cuts at: 385 1743 1991 385
Size: 1358 248 2707
Fragments arranged by size:
2707 1358 248
KpnI G_GTAC'C
Cuts at: 1333 1333
Size: 4313
MluI A'CGCG_T
Cuts at: 3746 3746
Size: 4313
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1283 2984 3168 1283
Size: 1701 184 2428
Fragments arranged by size:
2428 1701 184
MscI TGG'CCA
Cuts at: 605 1060 1174 2074 605
Size: 455 114 900 2844
Fragments arranged by size:
2844 900 455 114
MslI CAynn'nnrTG
Cuts at: 628 1066 1105 2302 2429 628
Size: 438 39 1197 127 2512
Fragments arranged by size:
2512 1197 438 127 39
MspA1I CmG'CkG
Cuts at: 909 1048 1424 1738 2098 3111 3356 909
Size: 139 376 314 360 1013 245 1866
Fragments arranged by size:
1866 1013 376 360 314 245 139
NaeI GCC'GGC
Cuts at: 1248 2494 1248
Size: 1246 3067
NarI GG'CG_CC
Cuts at: 386 1744 1992 386
Size: 1358 248 2707
Fragments arranged by size:
2707 1358 248
NcoI C'CATG_G
Cuts at: 107 623 107
Size: 516 3797
NgoMIV G'CCGG_C
Cuts at: 1246 2492 1246
Size: 1246 3067
NspI r_CATG'y
Cuts at: 151 1366 1866 2773 151
Size: 1215 500 907 1691
Fragments arranged by size:
1691 1215 907 500
PciI A'CATG_T
Cuts at: 147 2769 147
Size: 2622 1691
PflMI CCAn_nnn'nTGG
Cuts at: 345 424 345
Size: 79 4234
PfoI T'CCnGG_A
Cuts at: 182 182
Size: 4313
PmeI GTTT'AAAC
Cuts at: 1312 1312
Size: 4313
PmlI CAC'GTG
Cuts at: 462 885 462
Size: 423 3890
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3452 3484 3452
Size: 32 4281
PpuMI rG'GwC_Cy
Cuts at: 744 744
Size: 4313
PspOMI G'GGCC_C
Cuts at: 873 1232 873
Size: 359 3954
PstI C_TGCA'G
Cuts at: 1360 2045 1360
Size: 685 3628
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4313
PvuII CAG'CTG
Cuts at: 1048 1738 2098 1048
Size: 690 360 3263
Fragments arranged by size:
3263 690 360
RsrII CG'GwC_CG
Cuts at: 2508 2508
Size: 4313
SacI G_AGCT'C
Cuts at: 1327 1327
Size: 4313
SalI G'TCGA_C
Cuts at: 706 1350 706
Size: 644 3669
SapI GCTCTTCn'nnn_
Cuts at: 1219 2336 2546 1219
Size: 1117 210 2986
Fragments arranged by size:
2986 1117 210
SbfI CC_TGCA'GG
Cuts at: 1360 1360
Size: 4313
ScaI AGT'ACT
Cuts at: 3830 3830
Size: 4313
SexAI A'CCwGG_T
Cuts at: 1133 1133
Size: 4313
SfcI C'TryA_G
Cuts at: 33 1356 2041 3034 3225 33
Size: 1323 685 993 191 1121
Fragments arranged by size:
1323 1121 993 685 191
SfoI GGC'GCC
Cuts at: 387 1745 1993 387
Size: 1358 248 2707
Fragments arranged by size:
2707 1358 248
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4313
SmaI CCC'GGG
Cuts at: 1335 1335
Size: 4313
SmlI C'TyrA_G
Cuts at: 1468 2383 2875 3137 1468
Size: 915 492 262 2644
Fragments arranged by size:
2644 915 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4313
SphI G_CATG'C
Cuts at: 1366 1866 1366
Size: 500 3813
StuI AGG'CCT
Cuts at: 692 692
Size: 4313
StyI C'CwwG_G
Cuts at: 107 623 1103 1447 1801 107
Size: 516 480 344 354 2619
Fragments arranged by size:
2619 516 480 354 344
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 608 1896 2029 2488 2697 608
Size: 1288 133 459 209 2224
Fragments arranged by size:
2224 1288 459 209 133
TatI w'GTAC_w
Cuts at: 503 3828 503
Size: 3325 988
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 356 388 3830 3862 356
Size: 32 3442 32 807
Fragments arranged by size:
3442 807 32 32
Tth111I GACn'n_nGTC
Cuts at: 703 1135 2110 703
Size: 432 975 2906
Fragments arranged by size:
2906 975 432
XbaI T'CTAG_A
Cuts at: 1344 1344
Size: 4313
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1808 1808
Size: 4313
XmaI C'CCGG_G
Cuts at: 1333 1333
Size: 4313
Enzymes that do cut:
AarI AccI Acc65I AclI AcuI AflIII AgeI AleI
AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI
BanI BanII BbeI BbsI BbvCI BcgI BciVI BclI
BglI BglII BlpI Bme1580I BmrI BplI BpmI Bpu10I
BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI
BseYI BsgI BsiEI BsiHKAI BsmBI Bsp1286I BspEI BspHI
BspMI BsrBI BsrDI BsrFI BssSI BstAPI BstBI BstEII
BstXI BstYI BtgI BtgZI BtsI DraI DraIII DrdI
EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI
FalI FspI HaeII Hin4I HincII HindIII KasI KpnI
MluI MmeI MscI MslI MspA1I NaeI NarI NcoI
NgoMIV NspI PciI PflMI PfoI PmeI PmlI PpiI
PpuMI PspOMI PstI PvuI PvuII RsrII SacI SalI
SapI SbfI ScaI SexAI SfcI SfoI SgfI SmaI
SmlI SpeI SphI StuI StyI TaqII TatI TstI
Tth111I XbaI XcmI XmaI
Enzymes that do not cut:
AatII AfeI AflII AhdI AlfI AloI AscI AvrII
BaeI BfrBI BmgBI BmtI BsiWI BsmI BsrGI BssHII
BstZ17I Bsu36I ClaI CspCI EcoNI EcoRV FseI FspAI
HpaI MfeI NdeI NheI NotI NruI NsiI PacI
PasI PshAI PsiI PspXI PsrI SacII SanDI SfiI
SgrAI SnaBI SrfI SspI SwaI XhoI XmnI ZraI