Restriction map for
pF1KE0086
(Circular) (Six-base) MAPSORT of: pF1KE0086.seq Check: 7187 from: 1 to: 4688
pF1KE0086 4688 bp
With 182 enzymes: SgfI *
April 24, 2009 13:10 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 996 1233 996
Size: 237 4451
AccI GT'mk_AC
Cuts at: 1726 1726
Size: 4688
Acc65I G'GTAC_C
Cuts at: 1704 1704
Size: 4688
AclI AA'CG_TT
Cuts at: 4528 4528
Size: 4688
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 766 1410 2513 2945 3692 3960 766
Size: 644 1103 432 747 268 1494
Fragments arranged by size:
1494 1103 747 644 432 268
AfeI AGC'GCT
Cuts at: 424 719 424
Size: 295 4393
AflIII A'CryG_T
Cuts at: 2040 3144 4121 2040
Size: 1104 977 2607
Fragments arranged by size:
2607 1104 977
AgeI A'CCGG_T
Cuts at: 3076 3076
Size: 4688
AleI CACnn'nnGTG
Cuts at: 904 1642 904
Size: 738 3950
AlwNI CAG_nnn'CTG
Cuts at: 1002 1993 3560 1002
Size: 991 1567 2130
Fragments arranged by size:
2130 1567 991
ApaI G_GGCC'C
Cuts at: 191 301 355 1352 1528 191
Size: 110 54 997 176 3351
Fragments arranged by size:
3351 997 176 110 54
ApaLI G'TGCA_C
Cuts at: 463 3458 463
Size: 2995 1693
ApoI r'AATT_y
Cuts at: 1692 4041 4297 1692
Size: 2349 256 2083
Fragments arranged by size:
2349 2083 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 4688
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4688
AvaI C'yCGr_G
Cuts at: 165 184 301 354 721 1663 1708 165
Size: 19 117 53 367 942 45 3145
Fragments arranged by size:
3145 942 367 117 53 45 19
BamHI G'GATC_C
Cuts at: 1336 1713 1336
Size: 377 4311
BanI G'GyrC_C
Cuts at: 711 1543 1588 1704 2118 2366 2401 711
Size: 832 45 116 414 248 35 2998
Fragments arranged by size:
2998 832 414 248 116 45 35
BanII G_rGCy'C
Cuts at: 191 301 355 488 1352 1528 1702 2732 191
Size: 110 54 133 864 176 174 1030 2147
Fragments arranged by size:
2147 1030 864 176 174 133 110 54
BbeI G_GCGC'C
Cuts at: 1592 2122 2370 1592
Size: 530 248 3910
Fragments arranged by size:
3910 530 248
BciVI GTATCCnnnnn_n'
Cuts at: 2586 3347 4688 2586
Size: 761 1341 2586
Fragments arranged by size:
2586 1341 761
BclI T'GATC_A
Cuts at: 2208 2208
Size: 4688
BglI GCCn_nnn'nGGC
Cuts at: 112 214 112
Size: 102 4586
BglII A'GATC_T
Cuts at: 2203 4240 2203
Size: 2037 2651
BlpI GC'TnA_GC
Cuts at: 487 1440 1800 487
Size: 953 360 3375
Fragments arranged by size:
3375 953 360
Bme1580I G_kGCm'C
Cuts at: 145 172 191 301 355 467 1352 1528 2313
Size: 27 19 110 54 112 885 176 785
Cuts at: 2313 2406 3462 145
Size: 93 1056 1371
Fragments arranged by size:
1371 1056 885 785 176 112 110 93
54 27 19
BmgBI CAC'GTC
Cuts at: 462 603 462
Size: 141 4547
BmrI ACTGGGnnnn_n'
Cuts at: 940 1900 1981 2316 4460 940
Size: 960 81 335 2144 1168
Fragments arranged by size:
2144 1168 960 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3055 3055
Size: 4688
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 853 937 853
Size: 84 4604
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 616 1504 1864 2743 3235 3533 616
Size: 888 360 879 492 298 1771
Fragments arranged by size:
1771 888 879 492 360 298
BsaI GGTCTCn'nnnn_
Cuts at: 4392 4392
Size: 4688
BsaAI yAC'GTr
Cuts at: 2041 2041
Size: 4688
BsaBI GATnn'nnATC
Cuts at: 6 2202 4239 6
Size: 2196 2037 455
Fragments arranged by size:
2196 2037 455
BsaHI Gr'CG_yC
Cuts at: 280 860 1012 1589 2119 2367 3069 4565 280
Size: 580 152 577 530 248 702 1496 403
Fragments arranged by size:
1496 702 580 577 530 403 248 152
BsaWI w'CCGG_w
Cuts at: 1877 2398 3076 3350 3497 3908 1877
Size: 521 678 274 147 411 2657
Fragments arranged by size:
2657 678 521 411 274 147
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 355 1096 355
Size: 741 3947
BseYI C'CCAG_C
Cuts at: 190 1256 2113 2871 3448 3944 190
Size: 1066 857 758 577 496 934
Fragments arranged by size:
1066 934 857 758 577 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 436 676 1132 1242 436
Size: 240 456 110 3882
Fragments arranged by size:
3882 456 240 110
BsiEI CG_ry'CG
Cuts at: 104 233 1622 2230 2276 3484 104
Size: 129 1389 608 46 1208 1308
Fragments arranged by size:
1389 1308 1208 608 129 46
BsiHKAI G_wGCw'C
Cuts at: 467 1702 2480 2670 3462 467
Size: 1235 778 190 792 1693
Fragments arranged by size:
1693 1235 792 778 190
BsmBI CGTCTCn'nnnn_
Cuts at: 664 664
Size: 4688
Bsp1286I G_dGCh'C
Cuts at: 145 172 191 301 355 467 488 1352 1528
Size: 27 19 110 54 112 21 864 176
Cuts at: 1528 1702 2313 2406 2480 2670 2732 3462 145
Size: 174 611 93 74 190 62 730 1371
Fragments arranged by size:
1371 864 730 611 190 176 174 112
110 93 74 62 54 27 21 19
BspEI T'CCGG_A
Cuts at: 3908 3908
Size: 4688
BspHI T'CATG_A
Cuts at: 1070 3864 1070
Size: 2794 1894
BspMI ACCTGCnnnn'nnnn_
Cuts at: 764 996 1084 1233 1606 1738 1952 2254 2635
Size: 232 88 149 373 132 214 302 381
Cuts at: 2635 764
Size: 2817
Fragments arranged by size:
2817 381 373 302 232 214 149 132
88
BsrBI CCG'CTC
Cuts at: 50 125 333 1068 2980 3034 4518 4683 50
Size: 75 208 735 1912 54 1484 165 55
Fragments arranged by size:
1912 1484 735 208 165 75 55 54
BsrDI GCAATG_nn'
Cuts at: 729 2600 729
Size: 1871 2817
BsrFI r'CCGG_y
Cuts at: 1190 1473 2686 2867 3076 1190
Size: 283 1213 181 209 2802
Fragments arranged by size:
2802 1213 283 209 181
BssSI C'ACGA_G
Cuts at: 1172 1570 2959 3317 1172
Size: 398 1389 358 2543
Fragments arranged by size:
2543 1389 398 358
BstAPI GCAn_nnn'nTGC
Cuts at: 1002 2065 1002
Size: 1063 3625
BstBI TT'CG_AA
Cuts at: 3049 3049
Size: 4688
BstYI r'GATC_y
Cuts at: 1 1336 1658 1713 2180 2203 2538 2784 3796
Size: 1335 322 55 467 23 335 246 1012
Cuts at: 3796 3882 3894 4240 1
Size: 86 12 346 449
Fragments arranged by size:
1335 1012 467 449 346 335 322 246
86 55 23 12
BtgI C'CryG_G
Cuts at: 107 320 527 581 869 1344 1583 1643 107
Size: 213 207 54 288 475 239 60 3152
Fragments arranged by size:
3152 475 288 239 213 207 60 54
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 2638 2819 2982 2638
Size: 181 163 4344
Fragments arranged by size:
4344 181 163
BtsI GCAGTG_nn'
Cuts at: 625 851 1931 625
Size: 226 1080 3382
Fragments arranged by size:
3382 1080 226
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 570 605 570
Size: 35 4653
DraI TTT'AAA
Cuts at: 1687 1687
Size: 4688
DrdI GACnn_nn'nnGTC
Cuts at: 1572 2394 3252 1572
Size: 822 858 3008
Fragments arranged by size:
3008 858 822
EaeI y'GGCC_r
Cuts at: 230 524 569 818 1619 1653 2273 2447 2838
Size: 294 45 249 801 34 620 174 391
Cuts at: 2838 2865 4210 230
Size: 27 1345 708
Fragments arranged by size:
1345 801 708 620 391 294 249 174
45 34 27
EagI C'GGCC_G
Cuts at: 230 1619 2273 230
Size: 1389 654 2645
Fragments arranged by size:
2645 1389 654
EarI CTCTTCn'nnn_
Cuts at: 442 805 1147 2711 2921 442
Size: 363 342 1564 210 2209
Fragments arranged by size:
2209 1564 363 342 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 352 1041 2896 3206 3352 4498 352
Size: 689 1855 310 146 1146 542
Fragments arranged by size:
1855 1146 689 542 310 146
EcoICRI GAG'CTC
Cuts at: 1700 1700
Size: 4688
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 766 853 937 1410 2513 2945 3692 3960 766
Size: 87 84 473 1103 432 747 268 1494
Fragments arranged by size:
1494 1103 747 473 432 268 87 84
EcoNI CCTnn'n_nnAGG
Cuts at: 1558 1558
Size: 4688
EcoO109I rG'GnC_Cy
Cuts at: 298 1044 1096 1524 1827 298
Size: 746 52 428 303 3159
Fragments arranged by size:
3159 746 428 303 52
EcoRI G'AATT_C
Cuts at: 1692 1692
Size: 4688
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 1326 1617 2707 1326
Size: 291 1090 3307
Fragments arranged by size:
3307 1090 291
FspI TGC'GCA
Cuts at: 1558 2469 1558
Size: 911 3777
HaeII r_GCGC'y
Cuts at: 197 370 426 539 721 1592 2122 2370 3392
Size: 173 56 113 182 871 530 248 1022
Cuts at: 3392 4371 197
Size: 979 514
Fragments arranged by size:
1022 979 871 530 514 248 182 173
113 56
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 2530 2560 2562 2592 2530
Size: 30 2 30 4626
Fragments arranged by size:
4626 30 30 2
HincII GTy'rAC
Cuts at: 1161 1299 1497 1727 1161
Size: 138 198 230 4122
Fragments arranged by size:
4122 230 198 138
KasI G'GCGC_C
Cuts at: 1588 2118 2366 1588
Size: 530 248 3910
Fragments arranged by size:
3910 530 248
KpnI G_GTAC'C
Cuts at: 1708 1708
Size: 4688
MluI A'CGCG_T
Cuts at: 4121 4121
Size: 4688
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 3359 3543 3359
Size: 184 4504
MscI TGG'CCA
Cuts at: 571 820 2449 571
Size: 249 1629 2810
Fragments arranged by size:
2810 1629 249
MslI CAynn'nnrTG
Cuts at: 904 1117 1312 1642 2677 2804 904
Size: 213 195 330 1035 127 2788
Fragments arranged by size:
2788 1035 330 213 195 127
MspA1I CmG'CkG
Cuts at: 322 613 816 871 971 993 1002 1199 1268
Size: 291 203 55 100 22 9 197 69
Cuts at: 1268 1430 1799 2113 2473 3486 3731 322
Size: 162 369 314 360 1013 245 1279
Fragments arranged by size:
1279 1013 369 360 314 291 245 203
197 162 100 69 55 22 9
NaeI GCC'GGC
Cuts at: 2869 2869
Size: 4688
NarI GG'CG_CC
Cuts at: 1589 2119 2367 1589
Size: 530 248 3910
Fragments arranged by size:
3910 530 248
NcoI C'CATG_G
Cuts at: 107 107
Size: 4688
NgoMIV G'CCGG_C
Cuts at: 2867 2867
Size: 4688
NotI GC'GGCC_GC
Cuts at: 230 230
Size: 4688
NspI r_CATG'y
Cuts at: 1741 2241 3148 1741
Size: 500 907 3281
Fragments arranged by size:
3281 907 500
PasI CC'CwG_GG
Cuts at: 271 1047 271
Size: 776 3912
PciI A'CATG_T
Cuts at: 3144 3144
Size: 4688
PflMI CCAn_nnn'nTGG
Cuts at: 735 1245 735
Size: 510 4178
PfoI T'CCnGG_A
Cuts at: 914 914
Size: 4688
PmeI GTTT'AAAC
Cuts at: 1687 1687
Size: 4688
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3827 3859 3827
Size: 32 4656
PpuMI rG'GwC_Cy
Cuts at: 1096 1096
Size: 4688
PspOMI G'GGCC_C
Cuts at: 187 297 351 1348 1524 187
Size: 110 54 997 176 3351
Fragments arranged by size:
3351 997 176 110 54
PspXI vC'TCGA_Gb
Cuts at: 721 721
Size: 4688
PstI C_TGCA'G
Cuts at: 374 858 1007 1095 1735 2420 374
Size: 484 149 88 640 685 2642
Fragments arranged by size:
2642 685 640 484 149 88
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4688
PvuII CAG'CTG
Cuts at: 816 1002 1430 2113 2473 816
Size: 186 428 683 360 3031
Fragments arranged by size:
3031 683 428 360 186
RsrII CG'GwC_CG
Cuts at: 2883 2883
Size: 4688
SacI G_AGCT'C
Cuts at: 1702 1702
Size: 4688
SacII CC_GC'GG
Cuts at: 323 872 323
Size: 549 4139
SalI G'TCGA_C
Cuts at: 1725 1725
Size: 4688
SapI GCTCTTCn'nnn_
Cuts at: 2711 2921 2711
Size: 210 4478
SbfI CC_TGCA'GG
Cuts at: 1735 1735
Size: 4688
ScaI AGT'ACT
Cuts at: 4205 4205
Size: 4688
SfcI C'TryA_G
Cuts at: 33 370 854 1003 1091 1263 1731 2416 3409
Size: 337 484 149 88 172 468 685 993
Cuts at: 3409 3600 33
Size: 191 1121
Fragments arranged by size:
1121 993 685 484 468 337 191 172
149 88
SfoI GGC'GCC
Cuts at: 1590 2120 2368 1590
Size: 530 248 3910
Fragments arranged by size:
3910 530 248
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4688
SmaI CCC'GGG
Cuts at: 167 186 303 356 1710 167
Size: 19 117 53 1354 3145
Fragments arranged by size:
3145 1354 117 53 19
SmlI C'TyrA_G
Cuts at: 631 721 1519 1843 2758 3250 3512 631
Size: 90 798 324 915 492 262 1807
Fragments arranged by size:
1807 915 798 492 324 262 90
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4688
SphI G_CATG'C
Cuts at: 1741 2241 1741
Size: 500 4188
SrfI GCCC'GGGC
Cuts at: 167 167
Size: 4688
StuI AGG'CCT
Cuts at: 1553 1553
Size: 4688
StyI C'CwwG_G
Cuts at: 107 1822 2176 107
Size: 1715 354 2619
Fragments arranged by size:
2619 1715 354
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 2271 2404 2863 3072 2271
Size: 133 459 209 3887
Fragments arranged by size:
3887 459 209 133
TatI w'GTAC_w
Cuts at: 4203 4203
Size: 4688
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 920 952 4205 4237 920
Size: 32 3253 32 1371
Fragments arranged by size:
3253 1371 32 32
Tth111I GACn'n_nGTC
Cuts at: 2485 2485
Size: 4688
XbaI T'CTAG_A
Cuts at: 1719 1719
Size: 4688
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 828 1173 2183 828
Size: 345 1010 3333
Fragments arranged by size:
3333 1010 345
XhoI C'TCGA_G
Cuts at: 721 721
Size: 4688
XmaI C'CCGG_G
Cuts at: 165 184 301 354 1708 165
Size: 19 117 53 1354 3145
Fragments arranged by size:
3145 1354 117 53 19
Enzymes that do cut:
AarI AccI Acc65I AclI AcuI AfeI AflIII AgeI
AleI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI
BamHI BanI BanII BbeI BciVI BclI BglI BglII
BlpI Bme1580I BmgBI BmrI BplI BpmI BpuEI BsaI
BsaAI BsaBI BsaHI BsaWI BseRI BseYI BsgI BsiEI
BsiHKAI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI
BsrFI BssSI BstAPI BstBI BstYI BtgI BtgZI BtsI
CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI
Eco57MI EcoNI EcoO109I EcoRI FalI FspI HaeII Hin4I
HincII KasI KpnI MluI MmeI MscI MslI MspA1I
NaeI NarI NcoI NgoMIV NotI NspI PasI PciI
PflMI PfoI PmeI PpiI PpuMI PspOMI PspXI PstI
PvuI PvuII RsrII SacI SacII SalI SapI SbfI
ScaI SfcI SfoI SgfI SmaI SmlI SpeI SphI
SrfI StuI StyI TaqII TatI TstI Tth111I XbaI
XcmI XhoI XmaI
Enzymes that do not cut:
AatII AflII AhdI AlfI AloI AscI AvrII BaeI
BbsI BbvCI BcgI BfrBI BmtI Bpu10I BsaXI BsiWI
BsmI BsrGI BssHII BstEII BstXI BstZ17I Bsu36I ClaI
DraIII EcoRV FseI FspAI HindIII HpaI MfeI NdeI
NheI NruI NsiI PacI PmlI PshAI PsiI PsrI
SanDI SexAI SfiI SgrAI SnaBI SspI SwaI XmnI
ZraI